Current avian influenza reports in Australia
Oceania is the only continent free from clade 2.3.4.4.b HPAI H5N1 (April 2024). Wild migratory birds will be returning to Australia between August – November, and therefore this constitutes the highest risk period for a viral incursion.
If you see any sick or dead wild birds, or poultry including backyard chooks, it is imperative that you call the Emergency Animal Disease Watch Hotline – regardless of which state you are located in. 1800 675 888. A summary of all cases is available on Outbreak Portal
We have performed a comprehensive risk assessment of HPAI incursion and evaluation of the current surveillance system, commissioned by the Department of Agriculture, Fisheries and Forestry. An abridged version has been provided by Wildlife Health Australia.
The National Avian Influenza in Wild Birds Program continues to do surveillance across Australia for avian influenza. Enhanced surveillance by Michelle Wille and Marcel Klaassen in 2022 and 2023 demonstrated no evidence for HPAI H5N1 2.3.4.4b incursion into Australia. We have received funding from Wildlife Health Australia to again undertake surveillance in spring 2024.
Popular science article outlining the global situation and our response in Australia: Bird flu, human cases, and the risk to Australia and Chickens, ducks, seals and cows: a dangerous bird flu strain is knocking on Australia’s door
See more about our work on low pathogenic avian influenza, revealing the ecology of low pathogenic avian influenza (Strong host phylogenetic and ecological effects on host competency for avian influenza in Australian wild birds), and evolutionary genetics (Australia as a global sink for the genetic diversity of avian influenza A virus), and demonstrating how viruses enter and spread (Contrasting dynamics of two incursions of low pathogenicity avian influenza virus into Australia) in Australia
HPAI H7 outbreaks in 2024
Updated: 6 December 2024
New Zealand has reported its first (EVER!) HPAI H7N6 outbreak in a free range egg farm near Otago Peninsula on 2 Dec 2024. Thus far, 2 sheds on the same property are affected. No spread to further properties. This virus is unrelated to the HPAI H7 outbreaks in Australia earlier in 2024, so likely constitutes a novel evolution event. The genome sequence is available on GISAID (4 December). More information from MPI NZ
All Australian HPAI H7 outbreaks have been resolved.
HPAI H7N3 reported in 7 poultry farms near Meredith. The IPs are located in the Restricted and Control Areas in the Golden Plains Shire where movement restrictions were already in place. A property near Terang is also positive for HPAI H7N9, and is linked to the first IP in Meredith. Both a housing order and movement restrictions are in place. Updates from Agriculture Victoria.
HPAI H7N8 reported in two poultry farms in the Greater Sydney Basin. Updates from NSW DPI. H7N8 also detected in a farm in the ACT, directly linked to an outbreak in NSW, followed by a backyard flock located in the quarantine area. Updates from ACT EPSDD.
These outbreaks are the result of a low pathogenic domestic strain from Australian wild birds entering poultry production, where high pathogenicity evolved. All previous HPAI outbreaks in Australia have been due to domestic HPAI H7 viruses, including the HPAI H7N7 outbreak in Victoria in 2020. In response, the USA and Japan have imposed trade restrictions; in general trade restrictions are imposed in countries/regions with HPAI in poultry, or which use HPAI vaccines. To support the outbreak response, our teams are out catching ducks in Victoria, and will contribute samples and testing results previously collected to understand the viral burden and diversity of LPAI H7 in Victorian wild birds.
LPAI H9N2 reported in Western Australia. This outbreak is the result of a domestic strain from Australian wild birds entering poultry production. To manage the infection, the farmer culled the cohort thought to be infected and risk-based surveillance was conducted on the rest of the property for 4 weeks post the discovery of the infection, with no further virus was detected. To date, only H5 and H7 viruses have evolved to HPAI. LPAI H9N2 is found in Australia wild birds, and is endemic in Asian poultry.
A returned traveller tested positive for HPAI H5N1 clade 2.3.2.1a. Information is available in the Department of Health press release and a Promed post. Two previous human infections have been caused by this clade (previously in Nepal and India). Importantly, this is not the same clade of virus as is causing the global panzootic (clade 2.3.4.4b). Australia remains free from HPAI H5N1 clade 2.3.4.4.b.
What to do if you see sick or dead birds in Australia?
Video from the Northern Australia Biosecurity Strategy (NABS) [link]
What does a sick bird look like?
– Neurological signs such as loss of coordination and balance, trembling head and body, or twisting of the neck
– Lethargy and depression, unresponsiveness, lying down, drooping wings, dragging legs
– Closed and excessively watery eyes, possibly with opaque cornea or darkened iris (new sign associated with Gannets in current outbreak)
– Respiratory distress such as gaping (mouth breathing), nasal snicking (coughing sound), sneezing, gurgling, or rattling
Video of a dying Sandwich Tern, from Rijks et al. 2022. EID
Video of seabirds birds with HPAI at rehabilitation centres, from SANCOB
Official guidelines (Australia):
High Pathogenicity Avian Influenza clade 2.3.4.4b incursion risk assessment for Australia
Wildlife Health Australia: Highly Pathogenic Avian Influenza (HPAI) and Wild Animals in Australia: A RISK MITIGATION TOOLBOX FOR WILDLIFE MANAGERS (2024 update)
Wildlife Health Australia: Technical Issue Update – Global High Pathogenicity Avian Influenza Events (updated Sept 2023)
Wildlife Health Australia: Risk management advice for bird banders, wildlife rangers and researchers (updated May 2024)
Wildlife Health Australia: Advice for veterinarians and animal health professionals (updated May 2024)
Wildlife Health Australia: Advice for people who encounter sick or dead wild birds (updated May 2024)
Wildlife Health Australia: Communicates guide for managers of wildlife populations (version Dec 2023)
World Organisation for Animal Health: Risk management for people working with wild birds
World Organisation for Animal Health: Practical guide for authorised field responders to HPAI outbreaks in marine mammals, with a focus on biosecurity, sample collection for virus detection and carcass disposal.
World Health Organisation: Public health resource pack for countries experiencing outbreaks of influenza in animals: revised guidance
World Health Organisation: Public health resource pack for countries experiencing outbreaks of influenza in animals
The Risk of Avian Influenza in the Southern Ocean: a practical guide
Agreement on the Conservation of Albatrosses and Petrels – Guidelines for working with albatrosses and petrels during the high pathogenicity avian influenza (HPAI) H5N1 panzootic
Pacific Seabird Group: Highly Pathogenic Avian Influenza (HPAI) is a devastating wildlife disease that impacts seabird populations worldwide and warrants our attention and response
AUSVETPLAN response strategy avian influenza
AUSVETPLAN Guidance document: Risk based assessment of disease control options for rare and valuable animals
AUSVETPLAN Operational Procedures Manual Wild Animal Response Strategy
Australian Government, Department of Agriculture, Fisheries and Forestry: Information on Avian Influenza (bird flu)
Australian Government, Department of Agriculture, Fisheries and Forestry: Information for bird owners
Wildlife Health Australia: National Wildlife Biosecurity Guidelines
Farm Biosecurity: National Farm Biosecurity Manual for Poultry Production
Zoo and Aquarium Association Australia: Zoo and Aquarium Biosecurity
Australian Veterinary Association: Guidelines for veterinary personal biosecurity
Australian Department of Health and Aged Care information on Avian influenza in humans
Overview of Excersize Volare
If avian influenza emerges in Australia, outbreak details will be hosted on the Australian Government outbreaks portal (no information here currently)
More about what we are doing in Antarctica:
Comprehensive risk mapping was prepared, attempting to identify regions with the highest incursion risk and species that may be involved in viral introduction. The first detection of HPAI in the region occurred on South Georgia (British Antarctic) in October 2023 and the first detection on the Antarctic Peninsula occurred on March 2024. All data from the region are collated on our SCAR AWHN mortality database.
We have brought together all testing and observation data generated in the austral summer of 2022/23, prior to the first cases, now available as a preprint on bioRxiv
We will had members of the SCAR Antarctic Wildlife Health Network visiting various locations across the Antarctic during the austral summer 2023/24, facilitated by Intrepid. A second expedition, Australis, comprised of an international team and was supported by IAATO, was led by Meagan Dewar, to collect samples and learn more about the impact of HPAI on Antarctic wild birds. Our teams will be out again in summer 2024/25 to undertake mortality surveys and collect samples.
Global situation:
FAO situation report (global)
WOAH Situation reports (global)
EFSA Avian influenza overview March–June 2024 (European)
European Union Reference Laboratories dashboard (European)
RSPB: Avian influenza: a major thread to our struggling seabirds (UK)
APHIS 2022-2023 Detections of Highly Pathogenic Avian Influenza (USA)
Wildlife Health Information Sharing Partnership (USA)
Canadian Wildlife Health Cooperative dashboard (Canada)
Chilean Scernapesca dashboard (Chile)
Chilean SAG dashboard (Chile)
Brazilian Dashboard (Brazil)
Peruvian dashboard (Peru)
H5Nx Nexstrain portal with all relevant sequence data
Response plans and recommendations:
DEFRA: Mitigation strategy for avian influenza in wild birds in England and Wales.
Recommendations from the Invasive Species Council: High pathogenicity avian influenza in wildlife: Is Australia prepared?
Scientific Task Force on Avian Influenza and Wild Birds statement on: H5N1 High pathogenicity avian influenza in wild birds – Unprecedented conservation impacts and urgent needs
Mitigation strategy for avian influenza in wild birds in England and Wales
Scottish wild bird highly pathogenic avian influenza response plan
WOAH: Avian influenza: why strong public policies are vital
OFFLU: Southward expansion of high pathogenicity avian influenza H5 in wildlife in South America: estimated impact on wildlife populations, and risk of incursion into Antarctica
Summary of the FAO Global Consultations on Highly Pathogenic Avian Influenza
RSPB. Avian Influenza: a major threat to our struggling seabirds
EFSA: Guidance for reporting 2023 laboratory data on avian influenza
HAIRS risk statement: Avian influenza A(H5N1) in livestock
Human health risk:
A quadripartite risk assessment (14 Aug 2024) determined that the risk of infection with HPAI for the general population was low, and for occupationally exposed people (e.g. poultry or dairy workers) the risk was low to medium (but with high uncertainty). Prior to 2023, all human infections with HPAI have been in people interacting with birds, particularly poultry (chickens, turkeys and ducks), however in 2024, one human case was associated with dairy production. Human infections have occurred in Asia, Europe, Africa, North and South America. Reassuringly, no onward transmission between humans has been detected. Details on human cases are listed below (last updated ~ July 2023). Please verify information below with the latest information from WHO.
CDC: Global reported A(H5N1) human cases, January 2022 through April 25, 2024
Cases of gs/gd H5Nx in humans since 2020
– 85 human cases of 2.3.4.4. H5N6 in China 2014-2023. Most cases have confirmed link to poultry [aggregation]
– 7 poultry workers containing an outbreak of 2.3.4.4. H5N8 in poultry in Russia, 2020 [WHO notification, scientific article]
– 1 human case of 2.3.4.4.h H5N6 in Laos in 2021 [scientific article]
– 3 poultry workers infected with H5Nx in Nigeria in 2021. Likely to comprise environmental carriage rather than bona fide infection [news story]
– 1 human case of 2.3.2.1a H5N1 in India in 2022 [scientific article]
– 1 human case of 2.3.4.4b H5N1 in Viet Nam in 2022 [Vietnamese ministry of health, english language news story]
– 1 human case of 2.3.4.4b H5N1 in UK in 2022. Kept flock of ducks in the home. Likely to comprise environmental carriage rather than bona fide infection [scientific article]
– 2 poultry workers infected of 2.3.4.4b H5N1 in Spain in 2022. Likely to comprise environmental carriage rather than bona fide infection [scientific article]
– 1 poultry worker infected with 2.3.4.4b H5N1 in USA in 2022. Likely to comprise environmental carriage rather than bona fide infection [WHO notification]
– 2 human cases of H5N1 in China in 2022-23 [news story]
– 2 human cases of 2.3.2.1c H5N1 in Cambodia in 2023 [WHO notification]
– 2 poultry workers infected of 2.3.4.4b H5N1 in UK in 2023. One case likely comprises environmental carriage rather than bona fide infection [news story]
– 1 human case of 2.3.4.4b H5N1 in Ecuador in 2023 [WHO notification]
– 1 human case of 2.3.4.4b H5N1 in Chile in 2023 [WHO notification]
– 2 human cases of 2.3.4.4b H5N1 in the UK in 2023 [WHO information]
– 8 human cases of 2.3.2.1c H5N1 in Cambodia in 2024 [CDC information]
– 1 human case of 2.3.4.4b (unconfirmed) H5N1 in Vietnam in 2024 [CIDRAP information]
– >55 human cases of 2.3.4.4b H5N1 in the USA in dairy and poultry workers in 2024 [CDC website]
– 1 human case of 2.3.2.1a H5N1 in Australia in a return traveller from India in 2024 [Dept Health notification]
– 1 human case of H5N2 (pathotype undisclosed) in Mexico in 2024 [WHO notification][preprint]
– 1 human case of 2.3.4.4b H5N1 in Canada in 2024 [CBC]
Intersting paper outlining the challenges of interpreting qPCR detections in humans – infection or environmental contamination? https://www.sciencedirect.com/science/article/pii/S1201971223007063
In addition to human cases with H5Nx, there have been human cases with H3, H7, H9, H10.
HPAI in Dairy Cattle:
Information updated here 6 Dec 2024
>718 farms, 15 states. Human cases. Alpacas. Mice. Barn cats. From cows back into poultry.
https://www.aphis.usda.gov/livestock-poultry-disease/avian/avian-influenza/hpai-detections/livestock
> This dashboard contains not only information on detections, but is loaded with lots of resources and recommendations.
32 human cases, most linked to dairy cattle. People w/ animal exposures should take precautions, including wearing eye & respiratory protection. CDC human infection dashboard
Genomic Data are available in the North America H5Nx public NextStrain build: https://nextstrain.org/groups/moncla-lab/h5nx/north-america/ha?c=species_group
https://nextstrain.org/avian-flu/h5n1-cattle-outbreak/genome?c=division&m=div
Sequence data from HPAI H5N1 outbreak in cattle more easily available on GenBank.
https://ncbiinsights.ncbi.nlm.nih.gov/2024/05/23/avian-influenza-a-h5n1-virus-sequences/
Resources
FAO: Recommendations for the surveillance of influenza A(H5N1) in cattle
https://openknowledge.fao.org/items/4c29fcb1-67e2-4a37-a780-cb4fe0c9f253
CDC launched a new H5 Influenza Wastewater dashboard, combining WastewaterSCAN + state/local health dept data. Features interactive map & 6-week H5 detection table. Data is updated every Friday.
https://t.co/PLGqHMyVKR
CDC Wastewater dashboard
https://www.cdc.gov/flu/avianflu/h5-monitoring.html#waste
For interpretation, it is important to note that this dashboard shows results from influenza A testing. Influenza A virus is found in humans, horses, pigs, birds, cattle. This dashboard does not show results of only avian influenza, so interpretation should be done with care. i.e. just because there are high levels in some state, doesn’t mean there are high levels of HPAI in cattle in that state.
WastewaterSCAN
https://t.co/mCqjKmsLBy
Status Update: Traces of H5N1 Detected in Austin-Travis County detected in wastewater surveillance. Risk to public remains low
https://www.austintexas.gov/news/status-update-traces-h5n1-detected-austin-travis-county-detected-wastewater-surveillance-risk-public-remains-low
Joint FAO/WHO/WOAH preliminary assessment of recent influenza A(H5N1) viruses
https://www.who.int/publications/m/item/joint-fao-who-woah-preliminary-assessment-of-recent-influenza-a(h5n1)-viruses
Joint WHO/FAO/WOAH risk assessment. Risk to humans still considered low,
US H5N1 Outbreak Tracker by Students for Health Security – new dashboard
https://public.tableau.com/app/profile/students.for.health.security.2024/viz/USH5N1OutbreakTracker/Dashboard1
Highly Pathogenic Avian Influenza A(H5N1) Virus in Animals: Interim Recommendations for Prevention, Monitoring, and Public Health Investigations
https://www.cdc.gov/bird-flu/prevention/hpai-interim-recommendations.html
Technical Report: Highly Pathogenic Avian Influenza A(H5N1) Viruses
https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/h5n1-technical-report-06052024.htm
Antiviral Susceptibility Testing of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated From Dairy Cattle in the United States, 2024
https://www.ceirr-network.org/news/antiviral-susceptibility-testing-of-highly-pathogenic-avian-influenza-a-h5-n1-viruses-isolated-from-dairy-cattle-in-the-united-states-2024?utm_source=twitter&utm_medium=hootsuite&utm_term=ceirrnetwork&utm_content=6ca4015a-bdd6-486a-b3eb-8b663608613a
Rapid Qualitative Risk Assessment: The Risk to Dairy Cattle in Canada from Avian Influenza A(H5N1) in Dairy Cattle in the US 2024 08 14
https://cezd.ca/reports/rapidqualitativeriskassessmenttherisktodairycattleincanadafromavianinfluenzaAH5N1indairycattleintheus20240814?l=en-us
Rapid Qualitative Risk Assessment: The Risk to Dairy Cattle in Canada from Avian Influenza A(H5N1) in Dairy Cattle in the US 2024 08 14
https://cezd.ca/reports/rapidqualitativeriskassessmenttherisktodairycattleincanadafromavianinfluenzaAH5N1indairycattleintheus20240814?l=en-us
USDA’s control strategy:
https://www.agriculture.com/usda-aims-to-isolate-exhaust-h5n1-virus-in-dairy-herds-8657880
WOAH GF-TAD Americas teleconference
GF-TADs Meeting: Detection of HPAI in Ruminants and Humans in the USA – Americas (woah.org)
DEFRA (UK) have done an outbreak assessment for HPAI of avian origin in domestic livestock
https://www.gov.uk/government/publications/influenza-a-h5n1-of-avian-origin-in-domestic-livestock-in-the-usa
A good review of the stuation in the US, and implications for Great Britiain.
“The most likely routes of entry of this American H5N1 virus into Great Britain are via trade in bovine products from affected farms in the USA, or by migratory wild birds. There is no trade in live cattle.”
“Therefore, there could be a route of entry to Great Britain of virus through unpasteurised dairy products imported from affected farms in the USA, although the vast majority of dairy products from the USA are pasteurised, such as cheese and whey, along with smaller amounts of yogurt, condensed milk and dairy spreads”
USDA Actions to Protect Livestock Health From Highly Pathogenic H5N1 Avian Influenza
https://www.usda.gov/media/press-releases/2024/04/24/usda-actions-protect-livestock-health-highly-pathogenic-h5n1-avian
FDA Updates on Highly Pathogenic Avian Influenza (HPAI)
https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
APHIS has put in further guidelines around cattle movement to increase traceability
https://www.aphis.usda.gov/news/agency-announcements/aphis-bolsters-animal-disease-traceability-united-states
Avian Influenza A(H5N1) U.S. Situation Update and CDC Activities
Current Situation Highlights Importance of Preventive Measures for People with Exposures
https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/one-health-situation-update.htm
includes:
– Recommendations for Worker Protection and Use of Personal Protective Equipment (PPE) to Reduce Exposure to Novel Influenza A Viruses Associated with Severe Disease in Humans
– Results of antiviral susceptibility tests showing that H5N1 is susceptible to neuraminidase inhibitors.
USDA Confirms Cow-to-Cow Transmission a Factor in Avian Flu Spread
https://www.agweb.com/news/livestock/dairy/new-usda-confirms-cow-cow-transmission-factor-avian-flu-spread
Avian influenza A(H5N1) in dairy farms: An update on public health and food safety concerns
https://ncceh.ca/resources/blog/avian-influenza-ah5n1-dairy-farms-update-public-health-and-food-safety-concerns
Updates on Highly Pathogenic Avian Influenza (HPAI)
https://www.fda.gov/food/alerts-advisories-safety-information/updates-highly-pathogenic-avian-influenza-hpai
APHIS Recommendations for Highly Pathogenic Avian Influenza (HPAI) H5N1 Virus in Livestock For State Animal Health Officials, Accredited Veterinarians and Producers: https://www.aphis.usda.gov/sites/default/files/recommendations-hpai-livestock.pdf
“Producers should practice enhanced biosecurity, minimize animal movements, test animals before movement, and isolate animals moved on or off premises”
Detection of Highly Pathogenic Avian Influenza (H5N1) in Dairy Herds: Frequently Asked Questions
https://www.aphis.usda.gov/sites/default/files/hpai-dairy-faqs.pdf
WOAH Statement
https://www.woah.org/en/high-pathogenicity-avian-influenza-in-cattle/
USDA’s Testing recommendations for cattle
https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/h5n1-analysis-texas.htm
WHO webinar on public health risk of avian influenza in dairy cattle with lots of good stuff in there (06/05/2024)
2024_05_06_H5N1_epiwin.pptx
Scientific studies addressing influenza in (dairy cattle)
Download a copy of my Endnote Library (updated 5/12/2024) HPAI_Dec2024 Copy.enlp
Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows
https://onlinelibrary.wiley.com/doi/10.1111/irv.70048
Neutralization assay using luminescent virus-like particles. Detection of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses with the cow-derived H5N1 virus = potential efficacy of vaccines using current CVVs
Susceptibility of calf lung slice cultures to H5N1 influenza virus
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2432368
ex vivo lung slice cultures from calves provide a useful method to rapidly screen host susceptibility to a range of influenza A viruses
A single mutation in bovine influenza H5N1 hemagglutinin switches specificity to human receptors
https://www.science.org/doi/10.1126/science.adt0180
HPAI in cattle still preferentially binds to avian (vs mammalian) receptors. The switch to mammalian receptors can occur in 1 mutation: Gln226Leu.
POLYGENIC DETERMINANTS OF H5N1 ADAPTATION TO BOVINE CELLS
https://www.biorxiv.org/content/10.1101/2024.11.29.626120v1
Contribution of viral “internal” genomic segments of H5N1 B3.13 to bovine cells adaptation. Generated recombinants with dif genotypes: recombinant B3.13 viruses displayed faster replication kinetics in bovine cells compared to other IAV
The Thermal Stability of Influenza Viruses in Milk
https://www.mdpi.com/1999-4915/16/11/1766
Pasteurisation inactivates HPAI in milk. Also works for influenza D viruses.
Intramammary infection of bovine H5N1 influenza virus in ferrets leads to transmission and mortality in suckling neonates
https://www.biorxiv.org/content/10.1101/2024.11.15.623885v2
Its not just cows – lactating ferrets transmit HPAI H5N1 to suckling kits. Viral RNA titers high in milk over time and remained high in mammary gland tissue. Viral titres in dam nasal swabs delayed, and minimally present in oral swabs.
Enhanced encephalitic tropism of bovine H5N1 compared to the Vietnam H5N1 isolate in mice
https://www.biorxiv.org/content/10.1101/2024.11.19.624162v2
Comparing human case H5N1 from cows and from birds (Vietnam/2004) uniformly lethal in mice. Cow: resp tract + brain, CNS infection, Bird: only resp tract >> diff tissue tropism.
Avian influenza A (H5N1) virus in dairy cattle: origin, evolution, and cross-species transmission
https://journals.asm.org/doi/10.1128/mbio.02542-24
Review of origin, evolution and cross species transmission of HPAI H5N1 in dairy cattle.
Environmental stability of HPAIV H5N1 in raw milk, wastewater and on surfaces
https://www.biorxiv.org/content/10.1101/2024.10.22.619662v1.abstract
Half-life of H5N1 in raw milk (2.1 days), on polypropylene (1.4 days) and stainless-steel surfaces (1.2 days), wastewater (0.48 days). Detectable quantities of infectious virus could theoretically persist in refrigerated raw milk for 45 days.
Transmission of a human isolate of clade 2.3.4.4b A(H5N1) virus in ferrets
https://www.nature.com/articles/s41586-024-08246-7
A/Texas/37/2024 (TX/37) A(H5N1) virus isolated from dairy farm worker in Texas
- maintaining an avian-like receptor binding specificity
- robust systemic infection in ferrets, w high levels of virus shedding
- severe and fatal infection, characterised by viremia and extrapulmonary spread
- efficient transmission in a direct contact setting
- capable of indirect transmission via fomites
A human isolate of bovine H5N1 is transmissible and lethal in animal models
https://www.nature.com/articles/s41586-024-08254-7
A/Texas/37/2024 (TX/37) A(H5N1) virus isolated from dairy farm worker in Texas
- Effective replication in primary human alveolar epithelial cells, less efficiently in corneal epithelial cells.
- Lethal in mice & ferrets, spread systemically with high titres in respiratory & non-respiratory organs.
- Effectively transmitted in ferrets via respiratory droplets in 17%–33% of transmission pairs. 5/6 infected ferrets died.
- PB2-631L (encoded by bovine isolates), promoted influenza polymerase activity in human cells, suggesting a role in mammalian adaptation like PB2-627K (encoded by huTX37-H5N1).
- Bovine HPAI H5N1 viruses susceptible to polymerase inhibitors both in vitro & in mice.
Replication Kinetics, Pathogenicity and Virus-induced Cellular Responses of Cattle-origin Influenza A(H5N1) Isolates from Texas, United States
https://www.biorxiv.org/content/10.1101/2024.10.29.620905v1?ct=
A/Texas/37/2024 replicated more efficiently than A/bovine/Texas/24-029328-02/2024 in mammalian and avian cells. The high path, (and modfieid low path) human virus exhibited higher pathogenicity and efficient replication in infected C57BL/6J mice compared to the bovine strain.
Orogastric Exposure of Cynomolgus Macaques to Bovine HPAI H5N1 Virus Results in Subclinical Infection
https://www.researchsquare.com/article/rs-5182487/v1
Intranasal/intratracheal inoculation of H5N1 in macaques = systemic infection w mild and severe respiratory disease. Infection via orogastric route = subclinical, limited infection and seroconversion
Survivability of H5N1 Avian Influenza Virus in Homemade Yogurt, Cheese and Whey
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2420731
Preprint now available in press.. No viable H5N1 in spiked dairy used to make whey, cheese & yoghurt
Efficacy of oseltamivir and baloxavir against A(H5N1)-contaminated bovine milk in mice
https://www.researchsquare.com/article/rs-5119512/v1
Baloxavir treatment in mice has better disease outcomes compared to oseltamivir after being infected with milk containing H5N1. Better mouse survival, lower organ titres.
Dairy cows inoculated with highly pathogenic avian influenza virus H5N1
https://www.nature.com/articles/s41586-024-08166-6
Experimental infection of cattle with HPAI by the American team:
– Heifers inocculated by aerosol respiraotry route, mild clinical disease, but lesions+seroconvertion
– Cattle innoculated via intramammary route. Clinical disease, decreased rumen motility, changes to milk appearance, production losses, high levels of viral RNA detected in milk, virus isolation, lesions in mammary tissue, and seroconversion
Infectivity and persistence of influenza viruses in raw milk
https://www.medrxiv.org/content/10.1101/2024.10.10.24315269v1
An interesting take – they tested PR8 (lab strain of human influenza) in milk. infectious in raw milk for up to 5 days, viral RNA remained detectable and stable for at least 57 days, with no significant degradation. Pasteurization significantly reduced detectable viral RNA
Influenza A(H5N1) Virus Resilience in Milk after Thermal Inactivation
https://wwwnc.cdc.gov/eid/article/30/11/24-0772_article
PR8 and a diversity of H5N1 viruses spiked into in retail and unpasteurized milk revealed virus resilience under certain conditions, but are inactivated under pasteurisation conditions.
The mammary glands of cows abundantly display receptors for circulating avian H5 viruses
https://journals.asm.org/doi/10.1128/jvi.01052-24
Mammary glands of cows display receptors for 2.3.4.4b H5 viruses, but respiratory tract does not = may explain tropism for mammary glands
Hot topic: Epidemiological and clinical aspects of highly pathogenic avian influenza H5N1 in dairy cattle
https://www.sciencedirect.com/science/article/pii/S2666910224001534
Review of epidemiological & clinical aspects of HPAI H5N1 in cattle. Includes discussion on surveillance & control measures to implement within herds. Identifies areas where further research is needed.
Hot topic: Influenza A H5N1 virus exhibits a broad host range, including dairy cows
https://www.sciencedirect.com/science/article/pii/S2666910224001546
Review of host range of avian influenza, and infection dynamics in cattle.
Hot topic: Avian influenza subtype H5N1 in US dairy—A preliminary dairy foods perspective
https://www.sciencedirect.com/science/article/pii/S2666910224001522
Review of HPAI in milk and dairy, and impact of pasteurisation.
Rapid evolution leads to extensive genetic diversification of cattle flu Influenza D virus
https://www.nature.com/articles/s42003-024-06954-4
Up to 80% cattle seropositive for influenza D virus. Five genetic lineages. Reassortment of IDV in US, transboundary circulation in Europe. Higher rate of evolution and uncontrolled circulation, could facilitate its adaptation to humans.
H5N1 clade 2.3.4.4b dynamics in experimentally infected calves and cows
https://www.nature.com/articles/s41586-024-08063-y
Results from the FLI cow experimental infections and it is a tour-de-force.
- Calves: moderate nasal replication + shedding with no severe clinical signs. No transmission to sentinel calves
- Dairy cows: no nasal shedding, high fever, severe acute mammary gland infection with necrotizing mastitis. No systematic infection
- Milk production was rapidly and drastically reduced and the physical condition of the cows was severely compromised. Virus titers in milk rapidly peaked at 108 TCID50/mL
- both H5N1 euDG + B3.13 successfully replicated in cattle udders without respiratory spread
Thermal inactivation spectrum of influenza A H5N1 virus in raw milk
https://www.biorxiv.org/content/10.1101/2024.09.21.614205v1.abstract
Decay and thermal stability spectrum of HPAI H5N1 virus in raw milk: long term stability in raw 91 milk at 4oC but is rapidly inactivated by pasteurization
Wastewater Surveillance for Influenza A Virus and H5 Subtype Concurrent with the Highly Pathogenic Avian Influenza A(H5N1) Virus Outbreak in Cattle and Poultry and Associated Human Cases — United States,
https://www.cdc.gov/mmwr/volumes/73/wr/mm7337a1.htm
Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases — United States, 2024
https://www.annemergmed.com/article/S0196-0644(24)00437-2/abstract
Abridged version of an MMWR Morb Mortal Wkly Rep shared earlier.
Bovine Highly Pathogenic Avian Influenza Virus Stability and Inactivation in the Milk Byproduct Lactose
https://www.mdpi.com/1999-4915/16/9/1451
H5N1 virus stable for 14 days in a concentrated lactose solution under refrigerated conditions. Heat or citric acid treatments successfully inactivated the virus in lactose.
Personal Protective Equipment Guidance for Highly Pathogenic Avian Influenza H5N1 Should Be Adapted to Meet the Needs of Dairy Farm Workers
https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiae380/7758743?login=true
Michigan targeting dairy workers with simplified PPE guidance for improved likelihood of adherence. Recommend specifically addressing high-risk practices observed on dairy farms.
Detection and Monitoring of Highly Pathogenic Influenza A Virus 2.3.4.4b Outbreak in Dairy Cattle in the United States
https://www.mdpi.com/1999-4915/16/9/1376
Evaluation of HPAI antibodies in serum and milk and viral RNA in milk on dairy farms in Texas, Kansas, and Michigan. Positive correlation between paired serum and milk sample results. high diagnostic performance during the convalescent phase.
Avian and Human Influenza A Virus Receptors in Bovine Mammary Gland
https://wwwnc.cdc.gov/eid/article/30/9/24-0696_article
Publication of a preprint previously included. Detected IAV sialic acid -α2,3/α2,6-galactose host receptors in bovine mammary glands by lectin histochemistry. Results provide a rationale for high levels of H5N1 virus in milk from infected cows.
The highly pathogenic H5N1 virus found in U.S. dairy cattle has some characteristics that could enhance infection and transmission among mammals
https://www.nature.com/articles/s41684-024-01425-z
News and Views paper about the Ensfield nature paper.
Recent Bovine HPAI H5N1 Isolate is Highly Virulent for Mice, Rapidly Causing Acute Pulmonary and Neurologic Disease
https://www.biorxiv.org/content/10.1101/2024.08.19.608652v1
Inoculation of C57BL/6J and BALB/c mice with HPAI resulted in virus replication in the lung inducing severe respiratory disease, C57BL/6J mice infected with the bovine isolate also developed high virus titers in the brain. Bovine isolate possesses enhanced respiratory and neuroinvasive/neurovirulent properties
Inactivation of highly pathogenic avian influenza virus with high temperature short time continuous flow pasteurization and virus detection in bulk milk tanks
https://www.sciencedirect.com/science/article/pii/S0362028X24001339?via%3Dihub
Publication of a report previously included.
Very high virus titre in bulk tank milk from affected regions. Heating from 40°C to 72.5°C in 9.9 seconds reduced virus to undetectable levels. = Pasteurisation effective..
Notes from the Field: Health Monitoring, Testing, and Case Identification Among Persons Exposed to Influenza A(H5N1) — Michigan, 2024
https://www.cdc.gov/mmwr/volumes/73/wr/mm7329a4.htm?s_cid=mm7329a4_w
As of May 23, 2024, Michigan had largest number of affected dairy & poultry facilities linked to HPAI A(H5N1) outbreak. Active symptom monitoring and testing of exposed workers led to detection of 2nd and 3rd known dairy-associated HPAI A(H5N1) cases in 2024.
Enhanced replication of contemporary human highly pathogenic avian influenza H5N1 virus isolate in human lung organoids compared to bovine isolate
https://www.biorxiv.org/content/10.1101/2024.08.02.606417v1
Compared virus replication & host responses in human alveolar epithelium infected with HPAI H5N1 viruses. A/Vietnam/1203/2004 replicated most efficiently, followed by A/Texas/37/2024, then A/bovine/Ohio/B24OSU-342/2024 → cattle viruses just not that efficient at infecting humans compared to human viruses.
Bovine H5N1 influenza virus binds poorly to human-type sialic acid receptors
https://www.biorxiv.org/content/10.1101/2024.08.01.606177v1
Despite large number of birds and cattle affected, few human cases of 2344b. Here, evidence that avian and bovine H5N1 influenza virus binds poorly to human-type sialic acid receptors.
Influenza A(H5N1) Virus Infection in Two Dairy Farm Workers in Michigan
https://www.nejm.org/doi/full/10.1056/NEJMc2407264
“Case report on the human infections.
Highly pathogenic H5N1 avian influenza virus outbreak in cattle: the knowns and unknowns
https://www.nature.com/articles/s41579-024-01087-1
By Neumann and Kawaoka, so definitely the paper to read. Short and to the point summary of the current situation.
Strain-dependent variations in replication of European clade 2.3.4.4b influenza A(H5N1) viruses in bovine cells and thermal inactivation in semi-skimmed or whole milk
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.30.2400436
Strain-dependent differences of H5N1 in thermal inactivation, particularly in whole milk. All H5N1 viruses and H10 (with trypsin) replicated efficiently in bovine cells, but pigeon and red knot viruses exhibited lower titres,.
Fucosylated and non-fucosylated alpha2,3 sialosides were detected on the bovine mammary gland tissues
https://www.biorxiv.org/content/10.1101/2024.07.29.605565v1
Analyzed of the detailed receptor distributions of bovine mammary gland using recombinant HA’s rather than plant-derived lectins which have been used in other studies and may not be that appropriate.. Detection of fucosylated and nonfucosylated α 2,3 sialosides.
A One Health Investigation into H5N1 Avian Influenza Virus Epizootics on Two Dairy Farms
https://www.medrxiv.org/content/10.1101/2024.07.27.24310982v1
Sampled cattle, milk and workers. Found HPAI in cattle swabs, milk, but none in workers. Found antibodies against HPAI in some workers (small sample size). But.. they found SARS-CoV-2 in a nasal swab of a cow. What a mess.
Pop Sci: https://www.npr.org/sections/shots-health-news/2024/07/31/nx-s1-5059071/bird-flu-human-cases-farm-workers-testing
>>> ~15% farm workers tested (n=14) seropositive for HPAI in Texas. None in Michagan.
Repsonse from Florian Krammer: “This was measured by MN assay, not HAI. Titers of positives were low. Could as well be crossreactive anti-stalk or anti-N1 antibodies from seasonal H1N1 infections.: https://x.com/florian_krammer/status/1818732155733692690
Colorado orders weekly bulk tank avian flu testing for dairy farms
The state has now reported 49 outbreaks in dairy cows, nearly half of its licensed dairy farms
https://www.cidrap.umn.edu/avian-influenza-bird-flu/colorado-orders-weekly-bulk-tank-avian-flu-testing-dairy-farms
Notes from the Field: Health Monitoring, Testing, and Case Identification Among Persons Exposed to Influenza A(H5N1) — Michigan, 2024
https://www.cdc.gov/mmwr/volumes/73/wr/mm7329a4.htm
Evaluation of the Humoral Immune Response and Milk Antibody Transfer in Cattle vaccinated with inactivated H5 Avian Influenza vaccine
https://www.researchsquare.com/article/rs-4627508/v1
Calves inoculated with different vaccine doses, while lactating cows received the vaccine four weeks later. Dose-dependent immune response. Higher doses = stronger & more sustained antibody levels against HPAI 2.3.4.4b. Milk antibody transfer observed. Strong positive responses in milk samples by second week post-vaccination.
Spillover of highly pathogenic avian influenza H5N1 virus to dairy cattle
https://www.nature.com/articles/s41586-024-07849-4
Press release: https://www.eurekalert.org/news-releases/1052083
Paper includes a few preprints into 1 now and is probably the one to cite moving forward. Includes spillover, clincial signs, milk detection, tropism of mammary glands, multidirectional interspecies transmissions. This paper has gotten loads of media coverage, with “mammal-to-mammal transmission” in the title of almost every story.
Effectiveness of Pasteurization for the Inactivation of H5N1 Influenza Virus in Raw Whole Milk
https://www.medrxiv.org/content/10.1101/2024.07.23.24310825v1
By the Canaadian team – similar to other studies: complete inactivation of H5N1 spiked raw milk at 63C for 30 minutes. Complete viral inactivation observed in 7/8 replicates of raw milk samples treated at 72C for 15 seconds. Pasteurization effective.
CDC Birdflu Response
https://www.cdc.gov/bird-flu/spotlights/h5n1-response-07192024.html
>> Total of 10 human cases of 2.3.4.4b in USA – some from dairy cows, some from poultry
Experimental reproduction of viral replication and disease in dairy calves and lactating cows inoculated with highly pathogenic avian influenza H5N1 clade 2.3.4.4b
https://www.biorxiv.org/content/10.1101/2024.07.12.603337v1
Experimentally Holstein yearling heifers & lactating cows with cow HPAI. Heifers via aerosol respiratory route & cows by intramammary route. Mild clinical disease in heifers. Clinical disease in lactating cows = decreased rumen motility, changes to milk appearance, production losses consistent with field reports of viral mastitis.
Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm
https://link.springer.com/article/10.1007/s11262-024-02085-4
HPAIV H5N1 from environmental swab samples collected from a dairy farm in the state of Kansas, USA. PB2 E249G, NS1 R21Q pres, 1.7% reads w PB2 (E627K).
Genomic Characterization of Highly Pathogenic Avian Influenza A H5N1 Virus Newly Emerged in Dairy Cattle
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2380421
HPAI viruses from dairy cattle w abrupt milk drop, two cats, six wild birds, and one skunk = early identical genome sequences B3.13.
CDC A(H5N1) Bird Flu Response Update, July 5, 2024
https://www.cdc.gov/bird-flu/spotlights/h5n1-response-07052024.html
Stop H5N1 influenza in US cattle now
https://www.science.org/doi/10.1126/science.adr5866
Great piece by Nicola Lewis & Martin Beer: “Even difficult viral adaptations can occur if there are enough opportunities for contact and replication in new host species. Adaptation of the virus to cattle must be prevented.”
Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases — United States, 2024
https://www.cdc.gov/mmwr/volumes/73/wr/mm7321e1.htm?ACSTrackingID=USCDC_921-DM129096&ACSTrackingLabel=Early%20Release%20%E2%80%93%20Vol.%2073%2C%20May%2024%2C%202024&deliveryName=USCDC_921-DM129096
350 exposed farm workers being monitored; one of the two cases was identified via daily, active monitoring. Surveillance identified no unusual IAV activity trends in U.S.. A(H5) candidate vaccine viruses available. Lab analyses indicate A(H5N1) viruses circulating in cows & other animals susceptible to FDA-approved antivirals.
Surveillance of H5 HPAI in Michigan using retail milk
https://www.biorxiv.org/content/10.1101/2024.07.04.602115v2
qPCR detection of IAV in milk from local markets across Michigan. 2/13 samples +ve for IAV nucleic acid. Milk-based surveillance potentially useful if applied systematically at dairy processors or point of sale.
Inactivation of Avian Influenza Virus Inoculated into Ground Beef Patties Cooked on a Commercial Open-Flame Gas Grill
https://www.sciencedirect.com/science/article/pii/S0362028X24001091?via%3Dihub
USDA FSIS recommended minimum internal temperature of 71.1°C for ground beef reduced AIV levels to below detection.
Pathogenicity and transmissibility of bovine H5N1 influenza virus
https://www.nature.com/articles/s41586-024-07766-6
This article has garnered lots of attention – the results of mice and ferret experiments of H5N1. Viruses found in mammary glands of both mice and ferrets + resp tract of ferrets. Inefficient transmission in ferrets
Sci comm: Animal experiments shed more light on behaviour of H5N1 from dairy cows
https://www.cidrap.umn.edu/avian-influenza-bird-flu/animal-experiments-shed-more-light-behavior-h5n1-dairy-cows
Sci comm: https://www.nih.gov/news-events/news-releases/features-h5n1-influenza-viruses-dairy-cows-may-facilitate-infection-transmission-mammals
Health officials pitch anonymous bird flu testing
https://www.axios.com/2024/07/10/bird-flu-virus-cows-anonymous-testing
Farmers refusing to test herds fearing economic consequences. Anonymous testing could mean results from individual farms anonymized & sent to U.S. Department of Agriculture. Challenge of zeroing in & addressing transmission at source would still remain.
H5N1 avian influenza in USA: A call for vigilance in one health surveillance
https://www.sciencedirect.com/science/article/pii/S2590170224000293
Although immediate public health risk posed by H5N1 currently low, human infection in Texas reinforces importance of One Health approach.
Pasteurization Inactivates Highly Infectious Avian Flu in Milk
https://asm.org/Press-Releases/2024/July/Pasteurization-Inactivates-Highly-Infectious-Avian
Characterization of highly pathogenic avian influenza virus in retail dairy products in the US
https://journals.asm.org/doi/10.1128/jvi.00881-24
Publication of pre-print already reviewed
Inactivation of highly pathogenic avian influenza virus with high temperature short time 1 continuous flow pasteurization and virus detection in bulk milk tanks
https://www.fda.gov/media/179708/download?attachment
Evaluated HPAIV inactivation in artificially contaminated raw milk using most common legal conditions in US: 72°C for 15s. No viable virus detected.
Bird flu in wastewater
https://www.nature.com/articles/s41587-024-02297-x
Data from May 5–18 from 281 sampling locations show ^ levels of IAV virus at 3 sites in 2 states, & further 2 states where levels were ^ average compared with historic baseline samples.
H5N1 clade 2.3.4.4b avian influenza viruses replicate in differentiated bovine airway epithelial cells cultured at air-liquid interface
https://doi.org/10.1099/jgv.0.002007
Bovine cells + European HPAI H5N1: ????viral genome loads + infectious virus in 1st 24 h post-inoculation w/out cytopathogenic effects. infected cells still detectable by immunofluorescent staining @ 3dpi. =multiple lineages can infect resp tract of cattle
A single mutation in dairy cow-associated H5N1 viruses increases receptor binding breadth
https://www.biorxiv.org/content/10.1101/2024.06.22.600211v1
HPAI human case ^ binding to α2,3 sialic acids (avian receptor) compared to historical & recent 2.3.4.4b H5N1 viruses. No binding to α2,6 sialic acids yet (mammalian recept). Single mutation outside receptor binding site T199I responsible for increased binding breadth
CDC: H5N1 Bird Flu Confirmed in Person Exposed to Cattle
https://jamanetwork.com/journals/jama/article-abstract/2818256
Detection of A(H5N1) influenza virus nucleic acid in retail pasteurized milk
https://www.researchsquare.com/article/rs-4572362/v1
Testing of pasteurized milk from retail in USA: HPAI in 36.3%. No evidence of viable virus was found following inoculation of MDCK cells, embryonated chicken eggs, or mice.
H5N1 clade 2.3.4.4b avian influenza viruses replicate in differentiated bovine airway epithelial cells cultured at air-liquid interface
https://doi.org/10.1099/jgv.0.002007
Experimental infection of cow epithelial cells. 1st 24 hrs: ???? viral genome loads + infectious virus + no cytopathogenic effects. 3dpi: infected cells by immunoflourescnece. == can infect cattle resp tract.
Technical Report: June 2024 Highly Pathogenic Avian Influenza A(H5N1) Viruses
https://www.cdc.gov/bird-flu/php/technical-report/h5n1-06052024.html?CDC_AAref_Val=https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/h5n1-technical-report-06052024.htm
> Summary by Flu Trackers: https://flutrackers.com/forum/forum/national-international-government-ngo-preparation-response/cdc/h5n1-information/992090-cdc-a-h5n1-bird-flu-response-update-june-14-2024
> CDC analysed sera (blood) collected from people of all ages in all 10 HHS regions. Blood samples were collected during the 2022-2023 and 2021-2022 flu seasons. These samples were challenged with H5N1 virus to see whether there was an antibody reaction. Data from this study suggest that there is extremely low to no population immunity to clade 2.3.4.4b A(H5N1) viruses in the United States. Antibody levels remained low regardless of whether or not the participants had gotten a seasonal flu vaccination, meaning that seasonal flu vaccination did not produce antibodies to A(H5N1) viruses. This means that there is little to no pre-existing immunity to this virus and most of the population would be susceptible to infection from this virus if it were to start infecting people easily and spreading from person-to-person. This finding is not unexpected because A(H5N1) viruses have not spread widely in people and are very different from current and recently circulating human seasonal influenza A viruses.
Inactivation rate of highly pathogenic avian influenza H5N1 virus (clade 2.3.4.4b) in raw milk at 63 and 72 degrees Celsius
https://www.nejm.org/doi/full/10.1056/NEJMc2405488#
Press release summary: https://www.nih.gov/news-events/news-releases/infectious-h5n1-influenza-virus-raw-milk-rapidly-declines-heat-treatment
>heat treatment at 63°C would yield a decrease in infectious viral titer by a factor of 10^10 within 2.5 minutes = standard bulk pasteurization of 30 minutes at 63°C has a large safety buffer
> small but detectable quantity of HPAI A(H5N1) virus to remain infectious in milk after 15 seconds at 72°C if the initial titer is sufficiently high
Sialic Acid Receptor Specificity in Mammary Gland of Dairy Cattle Infected with Highly Pathogenic Avian Influenza A(H5N1) Virus
https://wwwnc.cdc.gov/eid/article/30/7/24-0689_article
The respiratory and mammary glands ofdairy cattle are rich in sialic acids, particularly avian α2,3-gal. Mammary gland tissues co-stained with sialic acids and influenza A virus nucleoprotein showed predominant co-localization with the virus and SA α2,3-gal.
Bird Flu Outbreak in Dairy Cows Is Widespread, Raising Public Health Concerns
https://jamanetwork.com/journals/jama/article-abstract/2818724
Summary of events in cattle, touching on all the key points.
Editorial: Concerns as Highly Pathogenic Avian Influenza (HPAI) Virus of the H5N1 Subtype is Identified in Dairy Cows and Other Mammals
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11155697/
Summary of epidemiology, transmission, and surveillance of HPAI H5N1 subtype in birds, mammals, and dairy cows, and why there are concerns regarding transmission to humans.
Avian Influenza A(H5N1) Virus among Dairy Cattle, Texas, USA
https://wwwnc.cdc.gov/eid/article/30/7/24-0717_article
Case report of early outbreak in cattle. Lots of interesting insights into how they worked it all out.
CDC Reports A(H5N1) Ferret Study Results
https://www.cdc.gov/flu/avianflu/spotlights/2023-2024/ferret-study-results.htm
- The A(H5N1) virus from the human case in Texas spread efficiently between ferrets in direct contact but did not spread efficiently between ferrets via respiratory droplets
- reinforce the need for people who have exposure to infected animals to take precautions
- No changes to risk assessment
Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases – United States, 2024
https://pubmed.ncbi.nlm.nih.gov/38814843/
2024 Highly pathogenic avian influenza (H5N1) – Michigan Dairy Herd and Poultry Flock Summary
https://www.aphis.usda.gov/sites/default/files/hpai-h5n1-dairy-cattle-mi-epi-invest.pdf
“Based on the epidemiological findings, the majority of links between affected dairy premises, and between dairy and poultry premises, are indirect from shared people, vehicles, and equipment.”
Highly Pathogenic Avian Influenza H5N1 Genotype B3.13 in Dairy Cattle: National Epidemiologic Brief https://www.aphis.usda.gov/sites/default/files/hpai-dairy-national-epi-brief.pdf
Canadians have updated (4 June) their milking pre-print. I expect there will be rolling updates.
Longitudinal Influenza A Virus Screening of Retail Milk from Canadian Provinces (Rolling Updates)
https://www.medrxiv.org/content/10.1101/2024.05.28.24308052v2
Does pasteurization inactivate bird flu virus in milk?
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2364732
Evaluation of thermal stability of HPAI H5N1, human H3N2 virus, H1, H3, H7, H9, H10. avian H3 virus = highest , HPAI = moderate thermal stability. standard pasteurization methods = effective.
Pasteurisation temperatures effectively inactivate influenza A viruses in milk
https://www.medrxiv.org/content/10.1101/2024.05.30.24308212v1
Pasteurisation effective for inactivation of human + avian influenza, influenza D, and recombinant IAVs carrying contemporary avian or bovine H5N1 glycoproteins. infectivity rapidly lost + undetectable before recommended pasteurisation time.
Cross-Species Transmission of Highly Pathogenic Avian Influenza (HPAI) H5N1 Virus in the U.S. Dairy Cattle: A Comprehensive Review
https://www.preprints.org/manuscript/202405.2137/v1
Yet another review of current epidemiological landscape of HPAI H5N1 in U.S. dairy cows and the recent interspecies transmission events of HPAI H5N1 in other mammals reported in other countries.
Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Viruses in U.S. Dairy Cattle and Detection of Two Human Cases — United States, 2024
https://www.cdc.gov/mmwr/volumes/73/wr/mm7321e1.htm
Cow’s Milk Containing Avian Influenza A(H5N1) Virus — Heat Inactivation and Infectivity in Mice
https://www.nejm.org/doi/full/10.1056/NEJMc2405495
Milk samples from HPAI H5 affected herd in New Mexico, viruses isolated. Heat treatment at 63°C reduced virus titers below the detection limit. HPAI remain infectious for several weeks in raw milk kept at 4°C. Infected mice had disease at 1dpi.
The mammary glands of cows abundantly display receptors for circulating avian H5 viruses
https://www.biorxiv.org/content/10.1101/2024.05.24.595667v3
2344b HPAI H5 from cattle bind significantly in the mammary gland, whereas classical H5 proteins failed to do so. 9-O-acetyl modification prominent in all tissues , 5-N-glycolyl modification is not. receptors are available in the lungs, and lower respiratory tract infections are often not efficiently transmitted and cause severe disease.
Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm
https://www.researchsquare.com/article/rs-4422494/v1
Isolation of HPAI H5N1 from environmental swab samples from a dairy farm in Kansas. Two distinctive mutations in the PB2 (E249G) and NS1 (R21Q) genes, 1.7% of reads w PB2 E627K. PB2 and NS most closely related to human case.
Longitudinal Influenza A Virus Screening of Retail Milk from Canadian Provinces (Rolling Updates)
https://www.medrxiv.org/content/10.1101/2024.05.28.24308052v1
Pan-Canadian Milk (PCM) Network: 8 retail milk samples from Canada (NL, NB, QC, MB, and AB) and all have tested negative for influenza A virus RNA. Routine surveillance of retail milk = cost-effective, standardized, scalable and easily accessible manner.
From birds to mammals: spillover of highly pathogenic avian influenza H5N1 virus to dairy cattle led to efficient intra- and interspecies transmission
https://www.biorxiv.org/content/10.1101/2024.05.22.595317v1
Brilliant paper addressing key questions in cattle HPAI. 3-20% cattle affected, disease 5-14 days. More shedding in clinical cattle. Staining of tissues = tropism for the epithelial cells lining the alveoli of the mammary gland. Genomic analysis reveals multidirectional interspecies transmissions. And they have mapped how virus spread between farms. This paper is worth the time.
Avian Influenza Virus (H5N1) Was Not Detected Among Dairy Cattle and Farm Workers in Pakistan
https://onlinelibrary.wiley.com/doi/full/10.1111/irv.13317
HPAI not detected in dairy cattle and farm workers in Pakistan. Samples originally collected for influenza D work. Some previous testing also in Canada and Germany, negative. Seems that infection in dairy cows localised to US (for now)
Detection of Hemagglutinin H5 Influenza A Virus Sequence in Municipal Wastewater Solids at Wastewater Treatment Plants with Increases in Influenza A in Spring, 2024
https://pubs.acs.org/doi/10.1021/acs.estlett.4c00331
Monitoring of 190 wastewater treatment plants across US ???? IAV RNA concentrations at 59 in spring 2024. Specific H5 testing showed increase due to H5 in 4 tested, all which catch discarded animal waste.
Characterization of highly pathogenic avian influenza virus in retail dairy products in the US
https://www.medrxiv.org/content/10.1101/2024.05.21.24307706v1
297 samples of Grade A pasteurised retail milk products (23 types) collected from 17 US states over 132 processors in 38 states. 20.2% positive by qPCR, all neg by egg innocs.
Influenza H5N1 and H1N1 viruses remain infectious in unpasteurized milk on milking machinery surfaces
https://www.medrxiv.org/content/10.1101/2024.05.22.24307745v1
HPAI H5N1 and H1N1 spiked into milk and put onto surfaces. HPAI remained infectious on surfaces for more than an hour = unpasteurized milk containing the H5N1 virus will remain infectious on milking equipment.
Virome Sequencing Identifies H5N1 Avian Influenza in Wastewater from Nine Cities.
https://www.medrxiv.org/content/10.1101/2024.05.10.24307179v1
19 of 23 monitored sites had at least one detection event, and the H5N1 serotype became dominant over seasonal influenza over time.
Preliminary report on genomic epidemiology of the 2024 H5N1 influenza A virus outbreak in U.S. cattle
(Part 1 of 2)
https://virological.org/t/preliminary-report-on-genomic-epidemiology-of-the-2024-h5n1-influenza-a-virus-outbreak-in-u-s-cattle-part-1-of-2/970?s=03
(Part 2 of 2)
https://virological.org/t/preliminary-report-on-genomic-epidemiology-of-the-2024-h5n1-influenza-a-virus-outbreak-in-u-s-cattle-part-2-of-2/971
This comprises the summary of genetic analysis of the sequences generated by USDA undertaken by evolutionary biologists. Highlights features such as reassortment prior to entering cattle, a single point of introduction into cattle, estimated date of virus introduction into the cattle population, mostly likely to have originated in Texas, and the presence of potentially adaptive mutations in cattle.
The avian and human influenza A virus receptors sialic acid (SA)-α2,3 and SA-α2,6 are widely expressed in the bovine mammary gland
https://www.biorxiv.org/content/10.1101/2024.05.03.592326v1
Turns out that cattle have avian type receptors in their mammary glands, which may explain why this is the “preferred” site of replication in cattle, and why there havent been to many occurences of the PB2 mutation in the cattle sequences.
Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in a Dairy Farm Worker
https://www.nejm.org/doi/full/10.1056/NEJMc2405371
Description of human case
Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus Infection in Domestic Dairy Cattle and Cats, United States, 2024
https://wwwnc.cdc.gov/eid/article/30/7/24-0508_article
Infected cattle experienced nonspecific illness, reduced feed intake and rumination, and an abrupt drop in milk production, but fatal systemic influenza infection developed in domestic cats fed raw (unpasteurized) colostrum and milk from affected cows.
Emergence and interstate spread of highly pathogenic avian influenza A(H5N1) in dairy cattle
https://www.biorxiv.org/content/10.1101/2024.05.01.591751v1
Great to see the analysis by the USDA and folks involved in the cattle outbreaks. Genomic analysis and epidemiological investigation showed a reassortment event in wild bird populations preceded a single wild bird-to-cattle transmission episode..
Potential Pathways of Spread of Highly Pathogenic Avian Influenza A/H5N1 Clade 2.3.4.4b Across Dairy Farms in the United States
https://www.medrxiv.org/content/10.1101/2024.05.02.24306785v1
Led by folks at the Kirby using spatial models to understand how spread may have occurred. Assumes that spread is occurring in real time.. Rather than identification of cases as more testing has been done.
Experimental Infection of Cattle with Highly Pathogenic Avian Influenza Virus (H5N1)
https://wwwnc.cdc.gov/eid/article/14/7/07-1468_article
4 calves experimentally infected with HPAI H5 (2006 strain). All remained healthy with no clinical signs. Limited shedding in nasal swabs, but shed low amounts of virus at 1dpi, 2dpi. All seroconverted.
Further Experiments Relating to the Propagation of Virus in the Bovine Mammary Gland
https://pubmed.ncbi.nlm.nih.gov/17648631/
From 1953: Human influenza type persisted
in the mammary gland for ~ 2 weeks, titres rose in milk and persisted for several days, neutralizing antibodies
detected soon after the virus had disappeared.
Studies relating to the formation of neutralizing antibody following the propagation of influenza and Newcastle disease virus in the bovine mammary gland
https://pubmed.ncbi.nlm.nih.gov/13316626/
A follow up, in 1955, w PR8 (these days a laboratory strain) (in goats as a sub for cattle). Infection in the mammary glad. Removal of mammary glad = decrease in antibody content in blood = neutralising antibodies being produced in mammary glad.
Significant rising antibody titres to influenza A are associated with an acute reduction in milk yield in cattle
https://sciencedirect.com/science/article/pii/S1090023307002419
Sporadic cases of “milk drop” investigated in a Holstein Friesian dairy herd in Devon, with increased antibody titres against human H3N2 and H1N1 associated with an acute fall in milk production
Influenza A in Bovine Species: A Narrative Literature Review
https://mdpi.com/1999-4915/11/6/561
There is a long history of influenza A in cattle. First case is 1949 with 160,000 cattle affected in Japan. Generally mammalian H1 and H3 subtypes implicated.
Influenza D virus
https://doi.org/10.1016/j.coviro.2020.08.004
Cattle are, of course, the central reservoir for influenza D viruses (a different virus species). Lots of scope for interesting work still to be done on influenza viruses in cattle.
Research articles, since ~ Nov 2022 (ongoing):
Follow me on Twitter/X for real-time publication sharing @DuckSwabber
Download a copy of my Endnote Library (updated 4/12/2024) HPAI_Dec2024 Copy.enlp
Incidence of highly pathogenic avian influenza H5N1 in pinnipeds in Uruguay
https://www.int-res.com/abstracts/dao/v160/p65-74/
In Uruguay, Sept-Dec 2023: 2713 stranded pinnipeds (S. American SeaLion, S. American Fur Seals); 92.4% were dead, including 80 aborted fetuses. Live animals w clinical signs: tremors, convulsions, extreme weakness.
Expansion of the early warning system for avian influenza in the EU to evaluate the risk of spillover from wild birds to poultry
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/sp.efsa.2024.EN-9114
– Review to identify the risk factors for virus introduction from wild birds into poultry farms and the availability of associated data in Europe.
– A theoretical modelling framework developed to assess, on a grid of 50 x 50 km cells, the relative weekly probability of HPAI introduction in at least one domestic poultry flock because of infectious wild birds.
Updated Biological Risk Assessment and Recommendations for Highly Pathogenic Avian Influenza in Antarctica
https://scar.org/~documents/route%3A/download/6304
Updated risk assessment and lessons learned from HPAI in Antarctica in 2023/24.
A 2022 avian H5N1 influenza A virus from clade 2.3.4.4b attaches to and replicates better in human respiratory epithelium than a 2005 H5N1 virus from clade 2.3.2.1
https://www.biorxiv.org/content/10.1101/2024.11.27.625596v1
2.3.4.4b viruses attach + replicated more efficiently in human respiratory epithelium than 2.1.3.2 (2005) virus. = increased risk of human infections with 2.3.4.4b = facilitate opportunities for human adaptation.
Influenza A(H5N1) shedding in air corresponds to transmissibility in mammals
https://www.nature.com/articles/s41564-024-01885-6
Based on air sampling in ferret trials: “kinetics data similar to ferret-to-ferret transmission studies. Absence of transmission observed for earlier A(H5N1) viruses due to a lack of infectious virus shedding in the air, not lack of mutations.
> 2005 zoonotic and 2024 bovine A(H5N1) viruses were not detected in the air.
> 2022 European polecat A(H5N1) virus and a 2024 A(H5N1) virus isolated from a dairy farm worker : shedding of infectious virus was observed for 1 out of 4 ferrets
Influenza A(H5N1) Virus Clade 2.3.2.1a in Traveler Returning to Australia from India, 2024
https://wwwnc.cdc.gov/eid/article/31/1/24-1210_article
HPAI H5N1 2.3.2.1a in traveller returning to Australia from India in 2024. Virus a reassortant including 2321a/2344b/LPAI. Demonstrates lack of sequence data from India, so hard to contextualise genomic data.
Pandemic preparedness of effective vaccines for the outbreak of newly H5N1 highly pathogenic avian influenza virus
https://www.sciencedirect.com/science/article/pii/S1995820X24001779
Huamn focus, and outlines current vaccines licensed for use in humans (n=14), adjuvants, and those in trials. Does mention need for better poultry vaccines and highlights China’s bivalent vaccine, but not much more.
Controlling minor outbreaks is necessary to prepare for major pandemics
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002945
Opinion – using H5N1 as a flagship example for the importance of controlling outbreaks.
TRPM2 deficiency ameliorated H9N2 influenza virus-induced acute lung injury in mice
https://www.sciencedirect.com/science/article/abs/pii/S0882401024006508
Mice with TRPM2 knocked out, infected w H9N2: lower levels of pulmonary oedema, lung permeability, Ca2+ concentration, redox imbalance, apoptosis, and levels of inflammatory factors. = therapeutic strategy for lung injury?
Chicken ANP32A-independent replication of highly pathogenic avian influenza viruses potentially leads to mammalian adaptation-related amino acid substitutions in viral PB2 and PA proteins
https://journals.asm.org/doi/full/10.1128/jvi.01840-24
HPAI viruses able to replicate in independently of ANP32A develop amino acid mutations that we associate with mammalian adaptation. Potential for ANP32A gene-edited chickens for HPAI control?
Age-Dependent Pathogenesis of Influenza A Virus H7N9 Mediated Through PB1-F2-Induced Mitochondrial DNA Release and Activation of cGAS-STING-NF-κB Signaling
https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.70062
In humans, why did H7N9 impact elderly? In mice, H7N9 PB1-F2 is a pathogenic factor in 15–18-month-old but not in 6–8-week-old. Triggers interferon β, chemokine gene expression, activation of cGAS-STING-NF-κB signaling
Origin, pathogenicity, and transmissibility of a human isolated influenza A(H10N3) virus from China
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2432364
H10N3 human case: virus generated in early 2019 in domestic poultry, binds to salic acid α2, 3 receptors, pathogenicity in BALB/c and C57BL/6 mice, respiratory droplet transmissibility in ferrets.
Reassortment of newly emergent clade 2.3.4.4b A(H5N1) highly pathogenic avian influenza A viruses in Bangladesh.
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2432351
In 2022-2023, H9N2 and 2321a H5N1 predominatnt in Bangladesh LBMs. Starting in 2023, emergence of 2344b HPAI – reassortants w 2344b and wild bird viruses. Will 2344b displace 2321a?
Highly Pathogenic H5N1 Influenza A Virus (IAV) in Blue-Winged Teal in the Mississippi Flyway Is Following the Historic Seasonal Pattern of Low-Pathogenicity IAV in Ducks
https://www.mdpi.com/2076-0817/13/11/1017
As ducks tend not to have mortality events like other species, monitoring for HPAI is best via serology. In teals, population level seroconversion in 2022 and against end of 2023 – two sweeps of HPAI. This paper also demonstrates that virus neutralisation is much better than HI, so I will aim to get this set up.
Emergence of HPAI H5N6 Clade 2.3.4.4b in Wild Birds: A Case Study From South Korea, 2023
https://onlinelibrary.wiley.com/doi/10.1155/tbed/4141478
In Dec 2023, a reassortant 2344b H5N6 detected in Korea, with N6 likely from 2.3.4.4h H5N6 prevalent in poultry in China (and associated human cases).
Genotypic Clustering of H5N1 Avian Influenza Viruses in North America Evaluated by Ordination Analysis
https://www.mdpi.com/1999-4915/16/12/1818
Since incursion of HPAI in Dec 2021, over 100 genotypes described in N. America. Need a way to analyse genotypes, not just HA phylogenies. Here ordination/cluster analysis reveals groupings and relationships
H5N1 Avian Influenza Outbreak Caused Massive Mortality of Chicks of Sandwich Tern Thalasseus sandvicensis in the Lagoon of Venice
https://doi.org/10.5253/arde.2024.a5
In 2022, HPAI decimated Sandwich Terns, particularly NW Europe. In 2023, outbreak of HPAI in Lagoon of Venice, killing all chicks. Adult mortality negligible.
Improved Influenza A Whole-Genome Sequencing Protocol
https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1497278/abstract
Great summary of influenza whole sequencing sequencing, and suggestions for improved workflows.
Update and narrative review of avian influenza (H5N1) infection in adult patients
https://accpjournals.onlinelibrary.wiley.com/doi/abs/10.1002/phar.4621
Thorough review of avian influenza in human patients: transmission, clinical presentation & complications, diagnosis, treatment, investigational therapies, infection control vaccination.
Single-dose avian influenza A(H5N1) Clade 2.3.4.4b hemagglutinin–Matrix-M nanoparticle vaccine induces neutralizing responses in nonhuman primates
https://www.biorxiv.org/content/10.1101/2024.11.21.624712v2.abstract
A recombinant 2344b vaccine: in mice induced robust antibody- and cell-mediated immune responses, in non-human primates induced pseudovirus neutralising titres.
Influenza A Virus Antibodies in Ducks and Introduction of Highly Pathogenic Influenza A(H5N1) Virus, Tennessee, USA
https://wwwnc.cdc.gov/eid/article/30/12/24-1126_article
One hypothesis about why ducks survive HPAI H5N1 without disease is the presence of LPAI H5 antibodies. Serology study shows high AIV antibodies, but low LPAI H5 antibodies (25%) in ducks in Tennessee prior to arrival of HPAI.
We are underestimating, again, the true burden of H5N1 in humans
https://doi.org/10.1136/bmjgh-2023-013146
Paper from last year: Are we underestimating the burden the H5N1 in humans? In Egypt, 4.5% of market workers are seropositive, but no reported infections to WHO. Prev of 2344b in workers, much higher than previous estimates of 2.2 viruses.
A call to innovate Antarctic avian influenza surveillance
https://www.sciencedirect.com/science/article/pii/S0169534724002787
Surveillance is hard, laborious, expensive. Even more so in the Antarctic. We highlight how to enhance our understanding of HPAI using innovative surveillance approaches.
Susceptibility of Mammals to Highly Pathogenic Avian Influenza: A Qualitative Risk Assessment From the Belgian Perspective
https://onlinelibrary.wiley.com/doi/10.1111/zph.13194
Comprehensive review of HPAI in mammals, with mutations, phenotypes, clinical consequences, spill over assessments. Super useful
A 2022 avian H5N1 influenza A virus from clade 2.3.4.4b attaches to and replicates better in human respiratory epithelium than a 2005 H5N1 virus from clade 2.3.2.1
https://www.biorxiv.org/content/10.1101/2024.11.27.625596v1.abstract
Being prepared for an avian influenza epidemic with a One Health approach: a cartographic study to identify animal carcasses burial sites in central Italy
https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/3475/1845
Development of a GIS-based approach for site identification for large-scale carcass disposal in Italy integrating data from geospatial sources, environmental restrictions + regulations, groundwater contamination, soil stability.
Avian raptors are indicator species and victims of high pathogenicity avian influenza virus HPAIV H5N1 (clade 2.3.4.4b) in Germany
https://www.nature.com/articles/s41598-024-79930-x
Raptors are important indicators of HPAIV + genetic diversity, but HPAI has substantial impacts on them with potential for serious negative impact on reproduction rates: Case study of white-tailed Eagles in Germany.
Genetic and pathological analysis of hooded cranes (Grus monacha) naturally infected with clade 2.3.4.4b highly pathogenic avian influenza H5N1 virus in South Korea in the winter of 2022
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1499440/full
In S. Korea 2022: 221 dead hooded cranes, 194 +ve for 2.3.4.4b H5N1 HPAI, 15 genomes. Similar to genomes from Japanese Cranes. Almost all Kor22-23C genotype. Histop+ immunohistochemical findings similar to other waterbirds.
Validation of a reduction in time for avian influenza virus isolation using specific pathogen-free embryonated chicken eggs
https://bvajournals.onlinelibrary.wiley.com/doi/10.1002/vetr.4842
If using virus isolation for diagnostics, could reduce incubation time of second passage.
Digitally immune optimised haemagglutinin with nanocage plug-and-display elicits broadly neutralising pan-H5 influenza subtype vaccine responses
https://www.biorxiv.org/content/10.1101/2024.11.14.623359v1
Need: broadly protective, future-proof vaccine against multiple clades of H5 influenza. Here, combined 2 novel vaxx tech. Vaccinated mice @ low doses: potent, cross-clade neutralising antibody and T cell responses against diverse H5 strains.
In Silico Genomic Analysis of Avian Influenza Viruses Isolated From Marine Seal Colonies
https://www.mdpi.com/2076-0817/13/11/1009
Review of avian influenzas found in seals, subtype diversity, avian-to-mammal-to-mammal transmission, reassortment before and in seals.
Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection
https://www.sciencedirect.com/science/article/pii/S0032579124011027
Expression profiles of long coding RNAs and targets in chickens infected with HPAI. ???? diff expressed long coding RNAs + mRNAs @ 1 +3 dpi, w resistant chickens ay stronger immune response than susceptibles @ 3 dpi
Epidemiological and Genetic Characterization of Human Infection with Avian Influenza AI H5N6 Virus in Guangxi, China, 2021
https://www.sciencedirect.com/science/article/pii/S120197122400359X
Human cases of H5N6 ticking along in China. In 2021, 11 cases, of which 10 2.3.4.4b. All viruses closely related to poultry-origin AIVs – entrechment of 2344b in Chinese poultry markets.
Dominant HPAIV H5N1 genotypes of Germany 2021/2022 are linked to high virulence in Pekin ducklings
https://www.nature.com/articles/s44298-024-00062-0
Epic study. In 2021/22, >15 genotypes in Germany. Experimental infections shows common genotypes had high virulence, rare genotypes = intermediate virulence. PB1 important in shaping virulence. More efficient replication for high virulence strain. Observations consistent with ‘virulence-transmission tradeoff’ model.
Epidemiological data of an influenza A/H5N1 outbreak in elephant seals in Argentina indicates mammal-to-mammal transmission
https://www.nature.com/articles/s41467-024-53766-5
Massive outbreak in S. Elephant Seals in Argentina, peak mortality 25 Sept – 10 Oct 2023. B3.2 genotype of 2.3.4.4b in a multinational marine mammal w D701N mutation. Slower evolutionary rate in mammals. Happy to finally see this one in print, reviewed previously as a preprint..
Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002916
Pseudovirus deep mutational scanning to reveal how mutations to 2.3.4.4b H5 HA affect phenotype, e.g. those which are important for binding to α2-6-linked sialic acids, HA stabilisation, neutralization by sera from mice and ferrets. Incredble!
Long-Term Dynamics of Different Avian Influenza Viruses in Mallard (Anas platyrhynchos) Population in Moscow City and Moscow Oblast: Dependence on the Migration Activity
https://link.springer.com/article/10.1134/S1062359024609418
Mallard faces, 2008–2023 from Moscow + Moscow oblast waterbodies. 2008–13 = European strains, 2014–19 = Asian strains. Frequency + diversity of viruses decreased sharply after 2014. >> changes in ratio of ducks hunted during spring & autumn, increased mallard numbers at winter grounds within the breeding range & reduced numbers of black-headed gulls
Reassortants of the Highly Pathogenic Influenza Virus A/H5N1 Causing Mass Swan Mortality in Kazakhstan from 2023 to 2024
https://www.mdpi.com/2076-2615/14/22/3211
1 Dec 2023 – 25 Jan 2024, 1132 (Whooper + Mute) swan corpses found in Lake Karakol, Khasakstan. More than one genotype present. Substitutions in PB2, PB1, NP, NS1 for enhanced virulence or adaptation in mammals
Prevalence and risk factors for avian influenza in backyard pigeons, ducks, and chickens in District Toba Tek Singh Pakistan
https://www.biorxiv.org/content/10.1101/2024.11.08.622629v1.abstract
2018-2019, Pakistan: 13.0% in backyard chickens, 7.7% in pigeons, 11.3% in domestic ducks. H7, H9, and HA/Untyped detected.
Are we serologically prepared against an avian influenza pandemic and could seasonal flu vaccines help us?
https://www.biorxiv.org/content/10.1101/2024.11.06.622244v1.abstract
Subtype-specific protection against H5N1 + H7N9 in human Spanish population limited or nonexistent. Seasonal vaccination able to ????antibody titers to protective levels in moderate percentage of people, probably due to cross-reactive responses.
Detection and characterization of highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b virus circulating in Argentina in 2023
https://www.sciencedirect.com/science/article/pii/S0325754124001159
14 Feb 2023: 1st HPAI in Argentina wild birds. B/w Feb – Aug: 113 (23.6%) notifications. 21 human cases suspected, but all negative. Argentinian viruses clustered together with those isolated in other countries of the region, multiple introductions.
Searching for high pathogenicity avian influenza virus in Antarctica
https://www.nature.com/articles/s41564-024-01868-7
Great to see the first results from the Australis expedition to survey Antarctica for HPAI. Ten sites visited, HPAI detected in Skuas at 4, including at a skua breeding colony. One sheathbill also positive.
Transmission dynamics of highly pathogenic avian influenza virus at the wildlife-poultry-environmental interface: A case study
https://www.sciencedirect.com/science/article/pii/S2352771424002581
Sampling for HPAI from sentinel Mallards, sediment samples, and samples within a 100 km radius IPs. HPAI found in wild birds before and after outbreaks in IPs. Serology showed H5 exposure in the sentinels with no disease signs.
The highly pathogenic avian influenza H5N8 sublineage 2.3.4.4b virus: A critical analysis of genetic steadiness based on full HA gene sequencing in Egypt
https://gmpc-akademie.de/articles/1731398217_gjvr-4-4-88.pdf
425 samples from wild + domestic birds in 13 provinces in Egypt, 2019-21. Prev 9.88% (n=32) in wild, 36.37% (n=64) in domestic, via egg iso. 8 HPAI H5N8 (coinfected w NDV).
Global risk mapping of highly pathogenic avian influenza H5N1 and H5Nx in the light of epidemic episodes occurring from 2020 onward
https://www.biorxiv.org/content/10.1101/2024.11.15.623755v1.abstract
Ecological niche modelling to elucidate environmental factors associated with ???? HPAI H5 since 2020: intensive chicken population density + cultivated vegetation, Spatial distribution influenced by urban areas + open water regions.
Molecular and In Vivo Characterization of the High Pathogenicity H7N6 Avian Influenza Virus That Emerged in South African Poultry in 2023
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/8878789
HPAI H7N6 virus emerged in S. African poultry in 2023, spread to Mozambique. 1st first documented emergence of H7 HPAI in Africa. 6.82 million birds culled, 20% of egg + 30% broiler flocks in RSA.
Dual receptor-binding, infectivity, and transmissibility of an emerging H2N2 low pathogenicity avian influenza virus
https://www.nature.com/articles/s41467-024-54374-z
H2N2 not seen in humans for decades, but is present in wild birds. H2 in domestic poultry in China different compared to A(H2N2)pdm1957. H2N2 rapidly adapts to mice + acquires mammalian-adapted mutations that facilitated transmission.
Novel reassortant H2N2 low pathogenic avian influenza virus in live bird markets in the Northeastern United States, 2019-2023
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2420712
H2N2 in the Northeast USA LBMs since 2014, with 3 subgroups, featuring intro from wild birds and reassortment. No molecular evidence of mammalian adaptation.
Highly pathogenic avian influenza H5N1 virus infections in pinnipeds and seabirds in Uruguay: Implications for bird–mammal transmission in South America
https://academic.oup.com/ve/article/10/1/veae031/7645834
HPAI genomes from marine mammals and seabirds in Uruguay, Sept 2023: pinnipeds most likely as the ancestral host, closely related to Peru (sea lions) and Chile (sea lions + human case), mammalian adaptative residues 591K and 701N
Immune history shapes human antibody responses to H5N1 influenza viruses
https://www.medrxiv.org/content/10.1101/2024.10.31.24316514v1
In humans, antibody titers to H5N1 highest in older individuals + correlate w year of birth = immune imprinting. Vaxx to A/Vietnam/1203/2004 = H5 reactive antibodies to 2344b + seroconversion in kids = younger individuals might benefit more from vaccination than older individuals in the event of a H5N1 pandemic.
Genotypic Clustering of H5N1 Avian Influenza Viruses Detected in North America
https://www.preprints.org/manuscript/202410.2401/v1
In North America: over 100 genotypes characterized via reassortment between HPAI and LPAI. ordination and cluster-based approaches can complement traditional phylogenetic analyses
Emergence of highly pathogenic avian influenza viruses H5N1 and H5N5 in white-tailed eagles, 2021–2023
https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.002035
HPAI H5N1 + H5N5 viruses detected in 31 white-tailed eagles in Norway, 2021-23. myocardial and splenic necroses, lesions in brain, multisystemic infection. 2 distinct incursions of HPAI Eurasian (EA) genotype C in eagles. Infected via predation.
Protective effects of an mRNA vaccine candidate encoding H5HA clade 2.3.4.4b against the newly emerged dairy cattle H5N1 virus
https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(24)00444-4/fulltext
BALB/c mice vaccinated with H5HA LNP-mRNA vaccine candidate containing HA from A/chicken/Ghana/AVL-76321VIR7050-39/2021. Vaxxed animals protected from cattle H5N1 virus without symptoms. Barely detectable serum neutralizing antibody titres suggest that T-cell responses induced by DS8390 are important.
A case study of the 2023 highly pathogenic avian influenza (HPAI) outbreak in tern (Sternidae) colonies on the east coast of the Republic of Ireland
https://www.tandfonline.com/doi/abs/10.1080/00063657.2024.2409196
Outbreak of HPAI in Ireland, 2023: Common Terns had highest mortality. Smaller numbers of Roseate Terns & Arctic Terns, few Sandwich Terns. Carcass removal was carried out at all affected colonies and most carcasses were incinerated
Low detection of H5N1 virus in commercial chickens with a low-level of vaccination coverage against H5N1 virus infection in Bangladesh
https://link.springer.com/article/10.1186/s42522-024-00119-3
Bangladesh: > 560 H5N1 outbreaks in poultry, 8 human cases since 2007. Across 5092 poultry farms, 25% vaccinated. 5% of tested farms had positive 2321c HPAI in chicken fecal samples. Protective effect of vaccination reported
Vaccine Quality Challenges in Secretly Traded H5 and H9 Avian Influenza Vaccines in Nigeria
https://www.ajol.info/index.php/nvj/article/view/281576
In Nigeria, lots of informal vaccination. Secretly imported vaccines lack of detectable HA antigen titer = imported H5 and H9 vaccines lack essential antigens that could stimulate antibodies = false sense of security for their flocks.
Characterization of H5N1 avian influenza virus isolated from bird in Russia with the E627K mutation in the PB2 protein
https://www.nature.com/articles/s41598-024-78175-y
In 2023 in Russia, isolated 2 HPAI viruses from emus, 1 contained PB2 E627K mutation. = increased virulence in mice, limited airborne transmission of the virus between ferrets, dominant binding to avian-type sialoside receptors
Seabird and sea duck mortalities were lower during the second breeding season in eastern Canada following the introduction of highly pathogenic avian influenza A H5Nx viruses
https://www.tandfonline.com/doi/full/10.1080/00063657.2024.2415161
Previously reviewed as a preprint, but now in print.
In eastern Canada: mortalities in 2023 93% lower than in 2022, but wider diversity of species incl waterfowl and seabirds. 3 notable mortality events 1646 Greater Snow Geese, 232 Canada Geese, 212 Northern Gannets
Emergence of a Novel Reassortant Clade 2.3.2.1c Avian Influenza A/H5N1 Virus Associated with Human Cases in Cambodia
https://www.medrxiv.org/content/10.1101/2024.11.04.24313747v1
Feb 2023-Aug 2024, Cambodia: 16 human cases of 2.3.2.1c H5N1. 14 reassortant H5N1 w gene segments from both clade 2.3.2.1c & clade 2.3.4.4b viruses. Persistent & ongoing threat of avian influenza in Southeast Asia.
Highly pathogenic avian influenza in wild mammals: critical appraisal of spill-over events and of strategies for prevention, surveillance and preparedness
https://zenodo.org/records/14002535
Overview of HPAI in mammals: HPAI transmission from birds > mammals and between mammals complex. Evaluation of wild mammals in different settings, including sylvatic environments, farms, and zoos/rescue centres and key factors influencing spillover.
Improved resolution of avian influenza virus using Oxford Nanopore R10 sequencing chemistry
https://journals.asm.org/doi/10.1128/spectrum.01880-24
Sequenced human and avian influenza genomes with R9 and new R10 chemistry: R10: increased data output, higher quality, improved performance in low sequence complexity regions, 90% resolution of HA cleavage site.
Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in Cats, South Korea, 2023
https://wwwnc.cdc.gov/eid/article/30/12/24-0154_article
In July 2023, HPAI reported at 2 shelters for stray cats in South Korea. Infection suspected from improperly sterilized raw food made from domestic duck meat. Viruses similar to South korean birds. E627K/D701N present. Systemic pathologic lesions
Intraductal infection with H5N1 clade 2.3.4.4b influenza virus
https://www.biorxiv.org/content/10.1101/2024.11.01.621606v1
Intraductal (ie, via udder) inoculation of H5N1 but not H1N1 influenza virus results in infection in mice
Incursion of Novel Eurasian Low Pathogenicity Avian Influenza H5 Virus, Australia, 2023
https://wwwnc.cdc.gov/eid/article/30/12/24-0919_article
Incursion of a LPAI H5 into Australia in ~2022. Early data suggest competitive exclusion, whereby no detections of previously circulating lineage in 2023. Useful to understand how HPAI may arrive.
Influenza A Virus H7 nanobody recognizes a conserved immunodominant epitope on hemagglutinin head and confers heterosubtypic protection
https://www.biorxiv.org/content/10.1101/2024.10.31.621368v1.abstract
Isolation and purification of a HA specific nanobody that recognizes H7 > broad-spectrum binding, cross-group neutralization and in vivo protection across various influenza A subtypes, targets an epitope on HA head
Comparative analysis of innate immune responses in Sonali and broiler chickens infected with tribasic H9N2 low pathogenic avian influenza virus
https://link.springer.com/article/10.1186/s12917-024-04346-8
A chicken is not a chicken is not a chicken
Sonali chickens exhibited higher proinflammatory and antiviral cytokine expressions in the trachea at 3–7 dpi against H9. broiler chickens showed lower immune responses, stronger early IFN-β responses and a delayed IFN-γ responses
Active Surveillance of Avian Influenza in the Southwestern Poyang Lake Area, China: Analyzing Changes in Wholesale and Frozen Fresh Retail Markets Post-Policy Implementation
https://www.sciencedirect.com/science/article/pii/S0032579124010642
From Oct 2020 – June 2024, 59.2% sammples in live poultry market in Poyang Lake Area, China positive for AIV. H9 most common, but H5 increasing trend. Implementing a frozen fresh poultry products policy effectively reduced AIV in first 2 years.
Dissecting the role of the HA1-226 leucine residue in the fitness and airborne transmission of an A(H9N2) avian influenza virus
https://journals.asm.org/doi/10.1128/jvi.00928-24
Limited capacity for airborne transmission observed in a human A(H9N2) virus isolate AL/39 which has a leucine at position HA1-226. Change L to Q, and loss of airbourne transmission, and ferrets had low susceptibility.
Molecular evolution of human infection with H9N2 subtype avian influenza virus in Anhui province from 2013 to 2022
https://rs.yiigle.com/cmaid/1518781
Genetic characterization of low-pathogenic avian influenza subtypes H10N6 and H10N7 from free-grazing ducks in Thailand
https://www.veterinaryworld.org/Vol.17/September-2024/25.php
640 swabs from 29 free ranging duck flocks in 6 provinces of Thailand: 6.41% positive for IAV: H10N6 and H10N7 detected.
A comprehensive epidemiological approach documenting an outbreak of H5N1 highly pathogenic avian influenza virus clade 2.3.4.4b among gulls, terns, and harbor seals in the Northeastern Pacific
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1483922/abstract
July 2023: ~1101 dead Caspian tern adults & 520 dead chicks in Pacific NW = 10-14% of Pacific Flyway population lost. 5 Habour Seals and 3% Glacous-winged Gulls affected.
Genetic and pathological analysis of hooded cranes (Grus monacha) naturally infected with clade 2.3.4.4b highly pathogenic avian influenza H5N1 virus in South Korea during 2022-2023
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1499440/abstract
In Nov/Dec 2022, 221 hooded cranes died in S. Korea. All viruses Kor22-23C genotype, similar to viruses from hooded cranes in Japan. Need for a cross-border cooperation effort.
Mass Mortality in Terns and Gulls Associated with Highly Pathogenic Avian Influenza Viruses in Caspian Sea, Kazakhstan
https://www.mdpi.com/1999-4915/16/11/1661
In 2022: 3200 dead Caspian terns, 850 Caspian gulls + 1200 Pallas’s gulls in Kazakhstan. Detected mutations that enhance virulence of H5N1 viruses in mammals
Phylogenetic Characterization of Novel Reassortant 2.3.4.4b H5N8 Highly Pathogenic Avian Influenza Viruses Isolated from Domestic Ducks in Egypt During the Winter Season 2021–2022
https://www.mdpi.com/1999-4915/16/11/1655
7 HPAI H5N8 viruses isolated from outbreaks of ducks in backyard & farm settings in Egypt. distinctive mutations in 5 genotypes within 1 genome constellation – potential enhancements in virulence, antiviral drug resistance, transmission in mammals..
A Rapid Virus‐Free Method for Producing Influenza HA Immunogen Needed for Preparation of Influenza Vaccine Potency Antisera Reagents
https://pmc.ncbi.nlm.nih.gov/articles/PMC11497102/
Production of recombinant influenza hemagglutinin from simple mammalian cell transfection to generate HA antibody (from two 2.3.4.4) suitable for use in influenza vaccine SRID potency assay.
The Novel 2.3.4.4b H5N6 Highly Pathogenic Avian Influenza Viruses Isolated From Wild Birds in 2023 Posing a Potential Risk to Human Health
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/4900097
H5N6 human infections in china, but rarely found in wild birds. In Dec 2023, reassortant 2.3.4.4b+H5N6 + H9N2 resurgent in wild birds and domestic ducks in Eastern Asia.
Out of the blue: Detection of a unique highly pathogenic avian influenza virus of subtype H7N5 in Germany.
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2420723
In June 2024, outbreak of HPAI H7N5 in 90,000 chicken layers in Germany. Cleavage site: PEIPKRKKRGLF. Sequences of all genome segments revealed similarities to avian influenza viruses from Asia and Europe
Optimized workflow for high-throughput whole genome surveillance of Influenza A virus
https://www.researchsquare.com/article/rs-5216720/v1
Improved AIV full genome sequencing: adjusting RT and PCR cycling conditions, = 1000-fold increase in sensitivity. Dual-barcoding for Nanopore = multiplexing w/o compromising sensitivity = scalable, high-throughput workflow for IAV surveillance.
Transmission of a human isolate of clade 2.3.4.4b A(H5N1) virus in ferrets
https://www.nature.com/articles/s41586-024-08246-7
A/Texas/37/2024 (TX/37) A(H5N1) virus isolated from dairy farm worker in Texas
- maintaining an avian-like receptor binding specificity
- robust systemic infection in ferrets, w high levels of virus shedding
- severe and fatal infection, characterised by viremia and extrapulmonary spread
- efficient transmission in a direct contact setting
- capable of indirect transmission via fomites
A human isolate of bovine H5N1 is transmissible and lethal in animal models
https://www.nature.com/articles/s41586-024-08254-7
A/Texas/37/2024 (TX/37) A(H5N1) virus isolated from dairy farm worker in Texas
- Effective replication in primary human alveolar epithelial cells, less efficiently in corneal epithelial cells.
- Lethal in mice & ferrets, spread systemically with high titres in respiratory & non-respiratory organs.
- Effectively transmitted in ferrets via respiratory droplets in 17%–33% of transmission pairs. 5/6 infected ferrets died.
- PB2-631L (encoded by bovine isolates), promoted influenza polymerase activity in human cells, suggesting a role in mammalian adaptation like PB2-627K (encoded by huTX37-H5N1).
- Bovine HPAI H5N1 viruses susceptible to polymerase inhibitors both in vitro & in mice.
Replication Kinetics, Pathogenicity and Virus-induced Cellular Responses of Cattle-origin Influenza A(H5N1) Isolates from Texas, United States
https://www.biorxiv.org/content/10.1101/2024.10.29.620905v1?ct=
A/Texas/37/2024 replicated more efficiently than A/bovine/Texas/24-029328-02/2024 in mammalian and avian cells. The high path, (and modfieid low path) human virus exhibited higher pathogenicity and efficient replication in infected C57BL/6J mice compared to the bovine strain. Whomever picked the strain name abbreviations should get a talking too as its impossible!
Seroprevalence of Avian Influenza in Guinea Fowls in Some Districts in the Upper East Region of Ghana
https://pubmed.ncbi.nlm.nih.gov/39474767/
Guinea fowl, important for agriculture in Ghana. 24.7% seropositive for AIV, higher seroprev in adults. Seroprev highest in birds from slaughter points.
Innate immune control of influenza virus interspecies adaptation via IFITM3
https://www.nature.com/articles/s41467-024-53792-3
IFITM3-deficient mice and human cells can be infected with low doses of avian influenza viruses that fail to infect wild type animals = IFITM3, a host antiviral factor may be controlling the minimum infectious virus dose threshold
From emergence to endemicity of highly pathogenic H5 avian influenza viruses in Taiwan
https://www.nature.com/articles/s41467-024-53816-y
Clade 2.3.4.4c arrived in Taiwan 2015/16 w localised outbreaks had multiple origins > persistance + re-emerging outbreaks in Yunlin county > endemicity.
First detection of a pathogenic avian influenza A/H5N1 clade 2.3.4.4b virus from laying hens in Northwestern Gabon
https://www.tandfonline.com/doi/pdf/10.1080/23311932.2024.2408850
In 2022, death of 17,498 laying hens in Gabon. 2.3.4.4b, 100% mortality rate. w 99% nucleotide similarity with the nigerian, benin and lesotho strains
Preparedness is key in the face of avian influenza uncertainty
https://www.sciencedirect.com/science/article/pii/S2052297524002890?via%3Dihub
Editorial: Recent case of human H5N2 infection in Mexico, reported on May 23, 2024, emphasizes the urgent need for robust surveillance and response strategies
Highly pathogenic avian influenza (HPAI) H5 virus exposure in domestic cats and rural stray cats, the Netherlands, October 2020 to June 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.44.2400326
Across 701 stray cats, 11.8% seropositive for HPAI, but only 0.46% domestic cats seropositive. Stray cats living in nature reserves + older cats more likely to be HPAI H5 seropositive
Genetic and pathogenic potential of highly pathogenic avian influenza H5N8 viruses from live bird markets in Egypt in avian and mammalian models
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0312134
2344bH5N8 HPAI isolated in 2021–22 in Egypt: mutations associated w increased virulence and polymerase activity in mammals, capable of replicating efficiently in mammalian cells lines, & in hamsters +mice without preadaptation causing systemic infection
A systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild avifauna of North America
https://royalsocietypublishing.org/doi/10.1098/rspb.2024.1845
Already reviewed as a repreprint. Brilliant resource: systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild birds. Resource available here: https://t.co/6xJHCrDhss
Are we prepared for the next pandemic: Monitor on increasing human and animal H5N1 avian influenza infection
https://journals.lww.com/cmj/fulltext/9900/are_we_prepared_for_the_next_pandemic__monitor_on.1290.aspx
Efficacy of the H7N9 vaccine as a candidate for the Korean avian influenza antigen bank
https://www.sciencedirect.com/science/article/abs/pii/S0165242724001375?via%3Dihub
Developed recombinant H7N9 vaccine (rgAPQAH7N9). Demonstrated robust immunogenicity in SPF chickens & complete protection, preventing clinical signs & viral shedding. Boosting the rgAPQAH7N9 vaccine yielded long-lasting immunity.
Phylogenetic Insights into H7Nx Influenza Viruses: Uncovering Reassortment Patterns and Geographic Variability
https://www.mdpi.com/1999-4915/16/11/1656
Analysed all available complete genomes of H7Nx viruses. Previously undescribed reassortment events identified in all subtypes. Reassortment between subtypes evident. In alignment with what we know about LPAI, so a bit meh.
Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024
https://www.mdpi.com/1999-4915/16/11/1668
Lombary, Italy 2022-24: 21 different subtypes, HPAI most common in 2022–23 winter (31.8%). H5 LPAI strains most prevalent (28.6%) in 2023–2024. 51% gulls positive for H5N1 to genotype BB in 2023.
Avian influenza overview June–September 2024
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/j.efsa.2024.9057
Latest EFSA summary article dropped. Certainly fewer notification in wild birds in 2024 compared to 2023/22, but colony breeders disproportionately affected. Detailed table on mammalian infections.
Comparative Inactivation of Three Different Subtypes of Avian Influenza Virus by Ozonized Water
https://doi.org/10.1637/aviandiseases-D-23-00058
Inactivation of avian influenza (H4N8, H4N6, and H9N9) by ozonated water (O3W) on seven different fomite = highly effective in 3 minutes on most fomites
Unexpected Delayed Incursion of Highly Pathogenic Avian Influenza H5N1 (Clade 2.3.4.4b) Into the Antarctic Region
https://onlinelibrary.wiley.com/doi/10.1111/irv.70010
Summary of HPAI surveillance in Antarctica in 2022/23 and 2023/24. Huge effort in coordination by @S_Lisovski to get this one across the line.
Risk Distribution of Human Infections with Avian Influenza A (H5N1, H5N6, H9N2 and H7N9) viruses in China
https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2024.1448974/abstract
Since 2021, 1,123 human infections w H5Nx, H9N2, H7N9 in China. Most cases have history of poultry exposure. Strong spatial distribution. Numerous risk factors identified – H5N6 associated with # live poultry markets
Genetic features of avian influenza (A/H5N8) clade 2.3.4.4b isolated from quail in Egypt
https://pubmed.ncbi.nlm.nih.gov/39396573/
HPAI H5N8 from commercial quail farm in Egypt. Simiar to 2.3.4.4b & recent H5N8 in Egypt. Presence of S123P, S133A, T156A & A263T = affinity for human-like receptors & increased virulence in mammals.
Effect of IgY Treatment on The Histopathological Finding in Tissue Sections of Ducks Naturally Infected with AI-H5N1
https://ejvs.journals.ekb.eg/article_386537.html
Outbreak of HPAI in Muscovy Ducks in Egypt, treated with purified egg yolk IgY. = stopped mortality and disease signs, improvement in general health conditions in birds. Birds injected with IgY showed milder lesions..
Survivability of low pathogenic avian influenza virus in aqueous poultry manure fertilizer
https://www.sciencedirect.com/science/article/pii/S1056617124000941
Given manure from poultry often used as fertiliser.. what about AIV survival after outbreaks? Processing poultry manure by aqueous extraction at temperatures ≥ 55°C for one hour completely destroyed LPAI.
Highly pathogenic avian influenza management policy in domestic poultry: from reacting to preventing
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.42.2400266?crawler=true
Challenges and opportunities in HPAI management policy in domestic poultry – Integrating regional biosecurity & poultry vaccination as prevention, need for structural reforms. Features France’s nationwide campaign targeting domestic ducks.
Multivalent interactions between fully glycosylated influenza virus hemagglutinins mediated by glycans at distinct N-glycosylation sites
https://www.nature.com/articles/s44298-024-00059-9
Cryo-EM structures of fully glycosylated HAs from H5N1 and H5N8 with detailed explanation of interactions, binding, and structure
How Do Flemish Laying Hen Farmers and Private Bird Keepers Comply with and Think about Measures to Control Avian Influenza?
https://www.mdpi.com/2306-7381/11/10/475
Perceived effectiveness of HPAI control in Belgium: self-reported compliance was high for professionals, but not private keerpers == need for information campaigns targetting private bird keepers.
Endangered Galápagos sea lions and fur seals under the siege of lethal avian flu: a cautionary note on emerging infectious viruses in endemic pinnipeds of the Galápagos Islands
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1457035/full
HPAI could have devastating impacts on places like the Galapagos. To date, HPAI not yet arrived, but potential epidemiological risks & health implications to endemic pinniped populations. Need for OneHealth measures in limiting exposure & spread.
Pre-existing H1N1 immunity reduces severe disease with bovine H5N1 influenza virus
https://www.biorxiv.org/content/10.1101/2024.10.23.619881v1
Pre-existing immunity from 2009 H1N1 pandemic could protect ferrets from mortality and severe clinical disease when infected with bovine H5N1 via differential tissue tropism. Antibodies from H1N1 infected ferrets are cross reactive with H5N1
Immune History Modifies Disease Severity to HPAI H5N1 Clade 2.3.4.4b Viral Challenge
https://www.biorxiv.org/content/10.1101/2024.10.23.619695v1
Mice and ferrets infected with 2009 H1N1/vaccinated with a live-attenuated influenza vaccine were moderately-to-highly protected against a H5N1 virus challenge. Need to explore both HA-inhibition or neutralizing antibodies and memory T cell responses.
Epidemiological characterization of human infection with H5N6 avian influenza
https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2024.1398365/full
While human cases of H5N1 are widely reported, there are cases of H5N6 in China each year. Of 24 cases addressed, 22 had history of contact with poultry. 12 fatalities = CFR 52.2%. Detailed case counts of H5N6 can be found here: https://bnonews.com/index.php/2021/10/tracking-human-cases-of-h5n6-bird-flu/
The H5N1-NS1 protein affects the host cell cycle and apoptosis through interaction with the host lncRNA PIK3CD-AS2
https://link.springer.com/article/10.1007/s11262-024-02118-y
NS1 is immune antagonist, but mechanism poorly understood. Identified host lncRNAs that interact with NS1 in vitro, PIK3CD-AS2 can directly interact and can inhibit transcription of NS1.
Genetic evolution, phylodynamic and phylogeographic of H5Ny AIVs in mammals
https://www.sciencedirect.com/science/article/pii/S2950248924000099
From 1997 = 2021, number of H5Ny mammalian infections low, small peak in 2006, sharp rise after 2022. Infection in mammals hampered by host body temperature, receptor distribution, pH value, and other factors, (eg ANP32)
The current situation with H5N1 avian influenza and the risk to humans
https://onlinelibrary.wiley.com/doi/10.1111/imj.16550
Review, with focus on human cases of avian influenza
Epidemiology and Biological Characteristics of Influenza A (H4N6) Viruses from Wild Birds
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2418909
H4N6 from wild birds in China – phylogeography showed Mongolia as transmission center for Eurasian lineage H4 viruses. All 9 viruses highly reassorted. Intersting combination of mutations e.g. mammalian virulence, dual receptor binding
Field production efficiency investigation of broilers immunized with a turkey herpesvirus vector vaccine expressing hemagglutinin from H9N2 subtype avian influenza virus
https://doi.org/10.1016/j.vaccine.2024.126436
When challenged with H9N2, immunisation with new Herpes Virus Vectored vaccine with expressing H9 blocked H9N2 AIV infection and reduced the mortality rate..
The host tropism of current zoonotic H7N9 viruses depends mainly on an acid-labile hemagglutinin with a single amino acid mutation in the stalk region
https://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1012427&s=03
HA2-116D of H7N9 confers zoonotic properties & important in viral infectivity & replication in human airway epithelial cells – increases pH sensitivity, cell membrane fusion in host cells & subsequent infection. HA2-116D not usually present in HA protein of H7 viruses with low zoonotic potential.
Genetic Characterization and Receptor Binding analysis of a Novel H5N1 HPAI Virus with a H6Nx-Derived PA Gene in Guangdong, China
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2417857
Detection of HPAI reassortant in asymptomatic geese in Foshan city, China, during LBM surveillance in 2023. Similar to H5N1 HPAIVs previously detected in wild birds in South Korea and Japan since 2022, PA similar to H6
Wastewater monitoring of human and avian influenza A viruses in Northern Ireland: a genomic surveillance study
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(24)00175-7/fulltext
Influenza A in wastewater in Ireland: detection of human and avian influenzas, including H13s and a 2344b-like NS segment. Analysis and interpretation of the avian viruses is not ideal. Key question is how is the detection of these avian viruses actionable?
NO EVIDENCE FOR HIGH PATHOGENICITY AVIAN INFLUENZA IN WAVED ALBATROSS PHOEBASTRIA IRRORATA
http://marineornithology.org/PDF/52_2/52_2_349-353.pdf
One critically endangered Waved Albatross found in Peru, positive for HPAI (May 2023). In response, surveillance of critically endangered Waved Albatross (n=51) on the Galapagos in 2023 for HPAI – all negative.
Highly Pathogenic Avian Influenza A(H5N1) Virus infection in dairy cattle:threat of bird flu has expanded to open-air farmed livestock
https://www.journalofinfection.com/article/S0163-4453(24)00245-7/fulltext
Substantial numbers of cattle in China, with farms spread across the country and in known migratory bird pathways. Good to consider whether cattle may be infected elsewhere and their future role.
Earliest Detection of Highly Pathogenic Avian Influenza (H5) in Mississippi Flyway Wild Waterfowl, 2022
https://meridian.allenpress.com/avian-diseases/article-abstract/doi/10.1637/aviandiseases-D-23-00033/503258/Earliest-Detection-of-Highly-Pathogenic-Avian?redirectedFrom=fulltext
Throwback to 2022, and potentially the first HPAI detections in Mississippi as early as January 2022, shortly after arrival of the virus to North America. 29% prevalence in ducks, and genotype A1 (genotype of first N. American detection)
New human H5N1 case: Should we worry? A genetic perspective
https://www.sciencedirect.com/science/article/pii/S2052297524002944?via%3Dihub
Detection of high pathogenicity avian influenza virus in Antarctica during the International HPAI Australis Expedition 2024 [Dataset]
https://digital.csic.es/handle/10261/369329
Dataset from international expedition undertaken from 13th March – 3rd April 2024 looking for signs of H5N1 clade 2.3.4.4b in seabirds & sea mammals throughout several locations in the South Shetland Islands, Trinity Peninsula & Northern Weddell Sea. If the dataset is available I expect the publication should be out soon.
A Machine Vision System for Monitoring Wild Birds on Poultry Farms to Prevent Avian Influenza
https://www.mdpi.com/2624-7402/6/4/211
Development of an AI model for automatic & real-time detection of wild birds on poultry farms. Potentially useful for identifying increased risk of HPAI???
Serological analysis in humans in Malaysian Borneo suggests prior exposure to H5 avian influenza near migratory shorebird habitats
https://www.nature.com/articles/s41467-024-53058-y#peer-review
Despite no human cases of HPAI in Malaysian Borneo, virus is in domestic + wild birds. Serology demonstrated H5 neutralisation in humans. 50% of the 178 samples were poultry owners (n = 89) and 33% (n = 58) lived within 10 km of migratory shorebird.
High pathogenicity avian influenza virus emergence: Blame it on chickens or on humans raising chickens?
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012608
A human monoclonal antibody targeting the monomeric N6 neuraminidase confers protection against avian H5N6 influenza virus infection
https://www.nature.com/articles/s41467-024-53301-6
Isolation of monoclonal against N6 (H5N6 is causing human infections in China). Confers protection in female mice. Elucidated a distinctive epitope on the NA, = illuminate dark side of NA in influenza immunity
An mRNA vaccine candidate encoding H5HA clade 2.3.4.4b protects mice from clade 2.3.2.1a virus infection
https://www.nature.com/articles/s41541-024-00988-9
Given inability to predict which H5 clade has pandemic potential, cross-clade protective HPAI H5 vaccines critical. Here, lipid nanoparticle mRNA vaccine can induce cross-protective immunity against HPAI
Fluorescent and bioluminescent bovine H5N1 influenza viruses for evaluation of antiviral interventions
https://journals.asm.org/doi/10.1128/jvi.01385-24
To facilitate rapid testing, production of fluorescent and bioluminescent variant strains using RG system. These strains useful in high-throughput evaluation of antiviral interventions
Human vaccination for highly pathogenic avian influenza
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736%2824%2902147-0/fulltext
SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection
https://www.sciencedirect.com/science/article/pii/S0925400524009262/pdf
An centrifugal microfluidic chip for RT/LAMP-CRISPR/Cas12a was designed for multiplex detection. Through smartphones & developed apps, the assay enabled portable, POC detection of 5 distinct influenza virus subtypes simultaneously (H1N1, H3N2, H5N1, H7N9 & influenza B virus).
Enhancement of protective efficacy of recombinant attenuated Salmonella typhimurium delivering H9N2 avian influenza virus hemagglutinins(HA) antigen vaccine candidate strains by C-C motif chemokine ligand 5 in chickens(chCCL5)
https://doi.org/10.1016/j.vetmic.2024.110264
Chicken C-C motif chemokine ligand 5 (chCCL5) significantly enhanced the protective effect of Salmonella delivering H9N2 AIV HA protein vaccine against H9N2 AIV infection.
Pathology and molecular detection of influenza A subtype H9N2 virus in commercial poultry in Nigeria, 2024
https://www.ejmanager.com/mnstemps/100/100-1717253573.pdf?t=1729209714
First reported H9N2 outbreak in commercial poultry in Southern Nigeria. Despite LPAI, virus has high virulence in chickens w hemorrhages, sinusitis, pneumonia, denudation of tracheal epithelia, parabronchial epithelial necrosis, airsac edema, emphysema
Could H5N1 bird flu virus be the cause of the next human pandemic?
https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1477738/abstract
Review focusing on H5N1 in cows. Authors consider H5N1 is unlikely to become a pandemic in the near future because the virus is yet to demonstrate efficient airborne transmission and still needs to acquire genetic traits known to facilitate widespread transmission to human populations.
First detection of a pathogenic avian influenza A/H5N1 clade 2.3.4.4b virus from laying hens in Northwestern Gabon
https://www.tandfonline.com/doi/full/10.1080/23311932.2024.2408850
Outbreak of HPAI in Gabon in 2022. 100% mortality rate observed in 17,498 laying hens. Sequences similar to other sequences from Africa, including those from Nigeria, Benin & Lesotho strains.
Intelligent prediction and biological validation of the high reassortment potential of avian H5N1 and human H3N2 influenza viruses
https://www.researchsquare.com/article/rs-4989707/v1
Deep learning framework to predict high-risk reassortment between avian and human IAVs, based adaptive codon contexts. Results of reassortment risk bw bovine HPAI H5N1 + human H3N2 validated with polymerase reporter assay
Updates to the wild bird abundance and movement models for the early warning system for avian influenza in the EU
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/sp.efsa.2024.EN-9000
Updates to “BirdFluRadar”, which is based on wild bird migration
https://app.bto.org/mmt/avian_influenza_map/avian_influenza_map.jsp
Pandemic risk characterisation of zoonotic influenza A viruses using the Tool for Influenza Pandemic Risk Assessment (TIPRA)
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(24)00234-9/fulltext
A(H7N9), A(H9N2), and A(H5) clade 2.3.4.4 viruses re-evaluated using TIPRA. A(H7N9) viruses had the highest relative risk. But, 2344b was evaluated in 2021, number of human cases and mammalian infections since…
Understanding the emergence of highly pathogenic avian influenza A virus H5N1 in pinnipeds: An evolutionary approach
https://www.sciencedirect.com/science/article/pii/S0168170224001655
Analysis of sequence data from marine mammals, globally. There are some issues with the trees. In alignment with expectation, pinniped sequences from different continents are in different clades. PB2 D701N in South America. PB2 E627K only found once, in a seal in Scotland.
Continuing evolution of H5N1 highly pathogenic avian influenza viruses of clade 2.3.2.1a G2 genotype in domestic poultry of Bangladesh during 2018–2021
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2403427
In 2018-21 in Bangladesh, 15.79% flocks positive for IAV, of which ~9% have HPAI H5N1. 15.8% of flocks positive for more than 1 virus. 2.3.2.1a genotype G2 viruses.
Molecular characteristics of the first human case infected with the avian influenza virus H5N1 clade 2.3.4.4b in eastern China
http://www.flu.org.cn/en/article_detail?action=ql&uid=&pd=&articleId=21796
Effects of Oral Exposure to HPAI H5N1 Pasteurized in Milk on Immune Response and Mortality in Mice
https://www.biorxiv.org/content/10.1101/2024.10.03.616493v1
In a mouse study, ingestion of inactivated HPAI H5N1 has limited potential health risk and does not prevent protective immune history-mediated responses to lethal infection.
Avian Influenza: Lessons from Past Outbreaks and an Inventory of Data Sources, Mathematical and AI Models, and Early Warning Systems for Forecasting and Hotspot Detection to Tackle Ongoing Outbreaks
https://www.mdpi.com/2227-9032/12/19/1959
Integrating technological advances, such as mathematical modeling and artificial intelligence to improve forecasting, hotspot detection, and early warning systems into a OneHealth approach for avian influenza.
Serological and Molecular Detection of Low Pathogenic Avian Influenza Subtype H9N2 in Commercial Poultry Flock in Kaduna and Kano State Nigeria
https://www.ajol.info/index.php/nvj/article/view/279540
Diversity of “pathogens” co-circulating in poultry in Nigeria. H9N2, E. coli & Klebsiella all detected. H9N2 anitbodies present. Clinical indications & lesions of Newcastle disease & E. coli septicemia. Results highlight co-infection with other pathogens could exacerbate infection.
Jamaican fruit bats mount a stable and highly neutralizing antibody response after bat influenza virus infection
https://www.pnas.org/doi/10.1073/pnas.2413619121
Infection of Jamaican fruit bats with H18N11 elicits rapid & stable humoral immune response with a strong neutralizing capacity. No detectable viral shedding after repeat challenge infection. Neutralizing antibody response of bats might play an important role in bat immunity.
A case study of highly pathogenic avian influenza (HPAI) H5N1 at Bird Island, South Georgia: the first documented outbreak in the subantarctic region
https://www.tandfonline.com/doi/full/10.1080/00063657.2024.2396563#abstract
High resolution outbreak tracking and management of HPAI on S. Georgia. Confirmed: 77 Brown Skuas, 38 Gentoo penguins, 58 Snowy Albatrosses, 5 dead Antarctic Fur Seals. Dramatic impacts on numerous populations of wild birds and mammals
Outbreaks of highly pathogenic avian influenza (HPAI) epidemics in Baltic Great Cormorant Phalacrocorax carbo colonies in 2021 and 2022
https://www.tandfonline.com/doi/full/10.1080/00063657.2024.2399168
Another HPAI “lowlight” – >1500 Great Cormorants died in Baltic/North Sea in 2021/22. Transmission of HPAI virus between neighbouring ground-nesting colonies. Affected scavenging White-tailed Eagles. No big pop level impact.
Microwave irradiation as a novel strategy for mitigating airborne transmission of highly pathogenic avian influenza A(H5N1) virus: an optimization study
https://www.researchsquare.com/article/rs-4929197/v1
Irradiation via microwave: efficacy is frequency and time dependent.
Broadly cross-reactive immune responses in chickens immunized with chimeric virus-like particles of nodavirus displaying the M2e originated from avian and human influenza A viruses
https://doi.org/10.1016/j.dci.2024.105275
Vacc of chickens with chimeric virus-like particles = robust induction of broadly reactive immune responses against both the M2e of avian & human IAVs. Chickens: IgY against the M2e of avian & human IAV, activated innate & adaptive immune responses.
Out of the blue: Detection of a unique highly pathogenic avian influenza virus of subtype H7N5 in Germany: Data sets on phylogenetic analyses
https://zenodo.org/records/13133875
Not just Australia – HPAI H7 outbreak in a single laying hen farm in Germany. Closest relatives of the eight viral genome segments were among recent LPAI viruses from Asia & Europe. Dataset fully available.
Avian influenza A H5N1 hemagglutinin protein models have distinct structural patterns re-occurring across the 1959–2023 strains
https://doi.org/10.1016/j.biosystems.2024.105347
How has the HA structure of H5 changed since 1997? 7 distinct structural patterns occurring, with differences in RBD. All 7 different patterns detected since emergence of gs/gd.
The Haemagglutinin Gene of Bovine Origin H5N1 Influenza Viruses Currently Retains an Avian Influenza Virus phenotype.
https://www.biorxiv.org/content/10.1101/2024.09.27.615407v1.abstract
HA of 2344b in UK birds and USA cattle: binding to avian-like receptors, pH fusion of 5.9 (outside range associated w efficient human airborne transmissibility). Little phenotypic difference of HA subs in cattle viruses = do not pose an enhanced threat
Molecular Characterization of a Clade 2.3.4.4b H5N1 High Pathogenicity Avian Influenza Virus from a 2022 Outbreak in Layer Chickens in the Philippines
https://www.mdpi.com/2076-0817/13/10/844
First full genome of 2344b from outbreaks in Phillipines. High mortality in layer chickens (96-100%). Most similar to other 2344b sequences in Asia.
Asymptomatic infection and antibody prevalence to co-occurring avian influenza viruses vary substantially between sympatric seabird species following H5N1 outbreaks
https://www.biorxiv.org/content/10.1101/2024.09.26.614314v1.abstract
Serology gives us a window into past exposure of birds against influenza. General anti-influenza A antibody levels high, particularly in kittiwakes (H13 and H16 at play here). But, antibodies against H5 are low in comparison (and almost absent against 2344b). Lots more opportunity for serology studies I think, to understand whether remaining seabirds have antibodies or not.
Novel Epidemiologic Features of High Pathogenicity Avian Influenza Virus A H5N1 2.3.3.4b Panzootic: A Review
https://onlinelibrary.wiley.com/doi/10.1155/2024/5322378
Changing epidemiology of gs/gd HPAI H5N1 – review
The practical longevity of stockpiled A(H5N1) influenza vaccine
https://www.nature.com/articles/s41591-024-03256-4
Summary/response to https://www.nature.com/articles/s41591-024-03189-y
WHO producing 3 candidate vaccine viruses (for human vaccines) against 2344b. Even if 2.3.4.4b viruses do not turn pandemic; such vaccines may be useful for the next waves in the coming decades. “Stockpiling A(H5) vaccines just makes sense.”
Detection of airborne wild waterbird-derived DNA demonstrates potential for transmission of avian influenza virus via air inlets into poultry houses, the Netherlands, 2021 to 2022
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.40.2400350
Airsampling of air-intakes in poultry barns shows this may be a source of introduction of HPAI through detection of avian DNA in poultry barns. Major issue here is that they didnt test for virus.. Just looked for bird eDNA, so unclear how to interpret this one.
First detection of influenza A virus subtypes H1N1 and H3N8 in the Antarctic region: King George Island, 2023
https://virusjour.crie.ru/jour/article/view/16674
“New” avian influenza subtypes in the antarctic – both H1 and H3 reported to be most similar to sequences from Eurasia, including Russia.
Avian influenza virus surveillance across New Zealand and its subantarctic islands detects H1N9 in migratory shorebirds, but not 2.3.4.4b HPAI H5N1
https://www.biorxiv.org/content/10.1101/2024.09.29.615640v1
Sampled wild birds of NZ outer islands & its subantarctic territories. Metatranscriptomic analysis of 700 individuals spanning 33 species revealed no detection of HPAI during annual 2023-2024 migration. A single detection of H1N9 in red knots (Calidris canutus).
Hemagglutinin and neuraminidase of a non-pathogenic H7N7 avian influenza virus coevolved during the acquisition of intranasal pathogenicity in chickens
https://link.springer.com/article/10.1007/s00705-024-06118-z
Study addressing how viruses go from LPAI to HPAI, and exactly what is needed. Just adding a MBCS into a LPAI virus doesn’t automatically make it HPAI. HA and NA undergo functional coevolution while acquiring high intranasal pathogenicity in chickens
Financial impact of low pathogenic avian influenza virus subtype H9N2 on commercial broiler chicken and egg layer production systems in Pakistan
https://doi.org/10.1016/j.prevetmed.2024.106346
Avian influenza has a considerable impact on poultry production, but what is that impact? Here, financial impact of LPAI H9N2 on commercial broiler chicken and egg layer production systems in Pakistan interrogated. Despite being LPAI, still considerable.
Exposure Practices to Animal-Origin Influenza A Virus at the Animal-Human Interface in Poultry and Swine Backyard Farms
https://pubmed.ncbi.nlm.nih.gov/39304348/
Interviews of people with backyard poultry in Chile: ????risk of influenza exposure if older, less educated, consumption of backyard poultry, higher backyard production. High influenza prevalence and seroprevalence in backyard animals.
Evidence of an emerging triple-reassortant H3N3 avian influenza virus in China
https://www.researchsquare.com/article/rs-4943745/v1
Sequenced an H3N3 from poultry in China, related to the H3N8 poultry sequences that have caused human infections in China.
Utilising citizen science data to rapidly assess changing associations between wild birds and avian influenza outbreaks in poultry
https://royalsocietypublishing.org/doi/10.1098/rspb.2024.1713
Intersting relationship between locations and timing of eBird sightings of some bird groups and HPAI outbreaks in poultry in the UK. == citizen science of bird observations may be useful in places with limited surveillance
The global H5N1 influenza panzootic in mammals
https://www.nature.com/articles/s41586-024-08054-z
Review of HPAI, w focus on molecular and ecological factors driving HPAIs sudden expansion in host range + assess likelihood of different zoonotic pathways leading to an potential pandemic. Also, really like this figure highlighting all the reassortment!
Challenges and Opportunities for Wastewater Monitoring of Influenza Viruses During the Multistate Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Virus in Dairy Cattle and Poultry
https://pubmed.ncbi.nlm.nih.gov/39298698/
Editorial from CDC folks. IAV testing on samples from >700 wastewater sites across 48 states, with testing occurring 1-3 x per week. No evidence of H5N1 in people in the states with high IAV levels in wastewater. 8/9 states with HPAI detections in wastewater in states with infected dairy herds.
Candidate Genes Associated with Survival Following Highly Pathogenic Avian Influenza Infection in Chickens
https://www.mdpi.com/1422-0067/25/18/10056
Difference in disease outcomes from the US H5N2 outbreak in 2015 (99% mortality). Number of host candidate genes that may be important in reduced disease severity identified, including ANP32A which has been featured in a number of recent Nature papers.
Highly Pathogenic Avian Influenza A (H5N1) Virus Outbreak in Ecuador in 2022–2024
https://link.springer.com/article/10.1007/s11908-024-00849-5
Reviewed of current situation of H5N1 epidemic in Ecuador HPAI detected in Ecuador as early as 24 Nov 2022. 10 poultry outbreaks, 17 backyard poultry outbreaks, 10 wild bird outbreaks across 6 species. 1 human case in Dec 2022. 8 sequences, total!.
Harnessing PROTACs to combat H5N1 influenza: A new frontier in viral destruction
https://onlinelibrary.wiley.com/doi/10.1002/jmv.29926
Review of antiviral therapeutics for influenza and proposal to consider use of PROTACs as a way forward.
On the avian influenza A (H7N5) outbreak: let’s not underestimate the less famous subtypes
https://www.tandfonline.com/eprint/R35RBCTVB4PJBSRS559W/full?target=10.1080/23744235.2024.2403707
Evolutionary characterization of the establishment of H6 influenza viruses in domestic geese in China: implications for the position of the host in the ecosystem
https://doi.org/10.1093/ve/veae075
109 H6 viruses isolated from domestic geese during 2001-2018 in southern China: related to viruses in ducks, 3 HA lineages, long-term persistence in geese, not often transmitted back to ducks or poultry
Epidemiology and evolution of human-origin H10N5 influenza virus
https://www.sciencedirect.com/science/article/pii/S2352771424002192
Another paper on the human H10 case. Wild bird-origin H10N5 influenza viruses from China did not cluster together with recent human H10N5 case. Human H10N5 : novel reassortant with wild bird viruses, spillover into humans.
Identification of key antigenic sites in hemagglutinin of H10N3 avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0032579124009222
3 monoclonal antibodies against the HA of H10N3 strain generated. Four novel antigenic sites identified = novel insights into the molecular markers for monitoring the antigenic variation
Molecular and phylogenic study of H9N2 avian Influenza virus in 2020 in six provinces of Iran
https://jpsad.com/index.php/jpsad/article/view/74
Broadening the aims of avian influenza surveillance according to the One Health approach
https://journals.asm.org/doi/full/10.1128/mbio.02111-24
“Canada’s AIV surveillance program in wild birds is an example for other countries. It is in line with FAO’s Global Consultation on Avian Influenza (14), where it was recommended to recognize HPAI not only as a concern for poultry production and public health but also as a concern for wildlife conservation; and to change goals, methods, and implementation of AIV surveillance, research, and response in wild birds accordingly at national, regional, and global levels”
Estimating adequate contact rates and time of Highly Pathogenic Avian Influenza virus introduction into individual United States commercial poultry flocks during the 2022/24 epizootic
https://www.biorxiv.org/content/10.1101/2024.09.08.611909v1.abstract
Modelling of HPAI in poultry: median most likely time to first positive sample = 6 days. median most likely adequate contact rate = 6.8 newly infected birds per infectious bird per day. R0 = 13.
Experimental Infection of Clades 2.2.1.2 (H5N1) and 2.3.4.4b (H5N8) of Highly Pathogenic Avian Influenza Virus Infection in Commercial broilers
https://www.sciencedirect.com/science/article/pii/S0147957124001061
Pathogenesis of H5N1 2.2.1.2 & H5N8 HPAI 2.3.4.4b in commercial broilers with maternal immunity: 100% mortality, neurological affinity with immune suppression. H5N8‐infected birds higher shedding.
Evolution and mutational landscape of highly pathogenic avian influenza strain A(H5N1) in the current outbreak in the USA and global landscape
https://www.sciencedirect.com/science/article/pii/S0042682224002678?via%3Dihub
An Update on Highly Pathogenic Avian Influenza A(H5N1) Virus, Clade 2.3.4.4b
https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiae379/7758741?login=true
Update touches on emergence of HPAI A (H5N1) Clade 2.3.4.4b, spread to mammals, epidemiology, diagnostic testing, antivirals, non-pharmaceutical interventions, vaccines and new developments.
Avian influenza H5N1 threatens imperiled krill-dependent predators in Antarctica
https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1453737/full
Considers risk of H5N1 to Antarctic pinnipeds extremely high. Commission for the Conservation of Antarctic Marine Living Resources considering time-area closures to limit interactions between krill fishery, Antarctic seabirds & marine mammals during biologically critical periods. Recommends adoption of conservation measures to reduce non-H5N1 mortality.
Influenza in feral cat populations: insights from a study in North-East Italy
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1439354/full
HPAI uncommon in feral cats in Italy – no qPCR positive cats, and only 1/279 serum samples positive for H5 antibodies. Cats sporadically infected elsewhere through consumption of “contaminated” food..
Genomic characterization of highly pathogenic H5 avian influenza viruses from Alaska during 2022 provides evidence for genotype-specific trends of spatiotemporal and interspecies dissemination
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2406291
Multiple introductions of HPAI into Alaska between November 2021 and August or September 2022. Genotypes differed in their spatiotemporal spread, likely influenced by timing of introductions relative to population immunity.
Recent Occurrence, Diversity, and Candidate Vaccine Virus Selection for Pandemic H5N1: Alert Is in the Air
https://www.mdpi.com/2076-393X/12/9/1044
VIRULENCE OF H5N1 INFLUENZA VIRUS IN CATTLE EGRETS (BUBULCUS IBIS)
https://meridian.allenpress.com/jwd/article/47/2/314/124162/VIRULENCE-OF-H5N1-INFLUENZA-VIRUS-IN-CATTLE-EGRETS
Cattle Egrets are not significant reservoir hosts for H5N1: experimentally infected birds highly susceptible -either died or had to be euthanized. Virus not transmitted to contacts.
Kleptoparasitism in seabirds—A potential pathway for global avian influenza virus spread
https://conbio.onlinelibrary.wiley.com/doi/full/10.1111/conl.13052
In addition to other established transmission routes, authors propose consideration of kleptoparasitism (stealing of food from other species) as a potential transmission route.
Vaccine optimization for highly pathogenic avian influenza: Assessment of antibody responses and protection for virus-like particle vaccines in chickens
https://www.sciencedirect.com/science/article/pii/S2590136224001256
Recombinant H5N2 vaccine based on virus-like particles against clade 2.3.4.4c (common in Taiwan) in chickens – all chickens survived lethal challenge. Reduced, but did not prevent viral shedding. .
M2e nanovaccines supplemented with recombinant hemagglutinin protect chickens against heterologous HPAI H5N1 challenge
https://www.nature.com/articles/s41541-024-00944-7
Combining nanoparticles with HA1 subunit vaccine in chickens: fully protected from clinical disease and mortality, and showed no histopathological lesions or virus shedding = complete cross-protection against HPAI H5N1 virus.
Acute and persistent responses after H5N1 vaccination in humans
https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01057-X
Comparative analysis of human responses to H5N1 vaccination with or without AS03 adjuvant. Independent of the adjuvant, vaccine-induced transcriptional patterns persist to at least 100 days after initial vaccination. Results suggest antigen-agnostic baseline immune states can be modulated by vaccine antigens alone to enhance future responses.
Human cases of avian influenza A(H5) in the USA
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(24)00239-8/fulltext
Letter discussing human cases of H5N1 in the US
Susceptibilities and viral shedding of peridomestic wildlife infected with clade 2.3.4.4b highly pathogenic avian influenza virus (H5N1)
https://www.sciencedirect.com/science/article/pii/S0042682224002526
Certain peridomestic species could pose a biosecurity threat to poultry operations. Following challenge HPAI found in starlings, sparrows, pigeons, skunks, opossums, cottontails.
Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b Infections in Seals, Russia, 2023
https://wwwnc.cdc.gov/eid/article/30/10/23-1728_article
In 2023, northern fur seals (3,500) and Steller sea lions died in the Far East region of Russia (Tyuleniy Island). 2344b similar to Asian viruses in Japan/Russia at that time. Not just American lineages resposible.
Pathogenicity of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Cats in Mice and Ferrets, South Korea, 2023
https://wwwnc.cdc.gov/eid/article/30/10/24-0583_article
In 2023, unusual deaths of cats at 2 animal shelters in Seoul, South Korea = due to 2344b. E627K or D701N PB2 mutation found. Cats infected by eating raw duck feed. Viruses lethal to mice and ferrets.
Wild bird mass mortalities in eastern Canada associated with the Highly Pathogenic Avian Influenza A(H5N1) virus, 2022
https://esajournals.onlinelibrary.wiley.com/doi/10.1002/ecs2.4980
Already reviewed as a preprint.
Sequencing-Based Detection of Avian Influenza A(H5N1) Virus in Wastewater in Ten Cities
https://www.nejm.org/doi/full/10.1056/NEJMc2405937
Between 4 March -15 July 2024, H5N1 detected in 10/10 cities and 100/399 samples in Texas waste water surveillance. Core challenge of OneHealth waste water surveillance: origin of the signal is currently unknown! Doesn’t correlate to human infections. Does water in cities reflect animal sources? Discarded infected milk? Infected birds in ponds? Wastewater is not a stand alone tool, and these results are not actionable (Although interesting).
Evaluation of Commercial RNA Extraction Protocols for Avian Influenza Virus Using Nanopore Metagenomic Sequencing
https://www.mdpi.com/1999-4915/16/9/1429
Comparison of RNA extraction approaches, paired with nanopore sequencing as a surveillance approach. Highlights how different RNA extraction protocols influence ONT sequencing performance.
Assessing the spatial risk of wild birds in avian influenza transmission using poly-species risk score
https://www.researchsquare.com/article/rs-4845265/v1
Investigation of risk of poultry farm outbreaks due to HPAIV from wild birds using Taiwan citizen scientist dataset. significant associations in 11 wild bird species, with shorebirds, raptors, ducks implicated.
Comparison of Extraction Methods for the Detection of Avian Influenza Virus RNA in Cattle Milk
https://www.mdpi.com/1999-4915/16/9/1442
Extracting RNA from cow’s milk is hard. Sample dilution 1:3 in molecular transport medium prior to RNA extraction provided the best results for dilution of inhibitory substances and a good recovery rate of influenza RNA.
Susceptibilities and viral shedding of peridomestic wildlife infected with clade 2.3.4.4b highly pathogenic avian influenza virus (H5N1)
https://doi.org/10.1016/j.virol.2024.110231
Susceptibility of Synanthropic Rodents (Mus musculus, Rattus norvegicus and Rattus rattus) to H5N1 Subtype High Pathogenicity Avian Influenza Viruses
https://www.mdpi.com/2076-0817/13/9/764
Rodents challenged with older clade H5N1 seem to get infected. No clinical signs, viruses isolated from oral swabs with virus found in respiratory tract tissues such as the nasal turbinates, trachea, and lungs. Wonder about impact of 2344b?
Proteomics Analysis of Duck Lung Tissues in Response to Highly Pathogenic Avian Influenza Virus
https://www.mdpi.com/2076-2607/12/7/1288
Previously reviewed preprint. Domestic ducks challenged with older clade H5N1. Activation of the RIG-I-like receptor and Jak-STAT signaling pathways -> protective anti-viral immune response in duck lung tissue
Understanding Ecological Systems Using Knowledge Graphs: An Application to Highly Pathogenic Avian Influenza
https://www.biorxiv.org/content/10.1101/2024.09.05.611483v1
Targets of influenza Human T cell response are mostly conserved in H5N1
https://www.biorxiv.org/content/10.1101/2024.09.09.612060v1
T cell epitopes conserved enough for cross-reactivity between avian H5N1 and human flu = N1 subtype encoded in both HPAI and seasonal H1N1 influenza virus as well as cross-reactive group 1 HA stalk-reactive antibodies
Characterization of Avian Influenza Viruses Detected in Kenyan Live Bird Markets and Wild Bird Habitats Reveal Genetically Diverse Subtypes and High Proportion of A(H9N2), 2018–2020
https://www.mdpi.com/1999-4915/16/9/1417
AIV Surveillance at Lake Victoria: poultry + poultry workers in LBMs. 3.9% pos, with 93% H9, similar to E. Africa viruses, Also H11 & LPAI H5N2. No poultry workers positive for AIV.
Characterization of Conserved Evolution in H7N9 Avian Influenza Virus Prior Mass Vaccination
https://www.tandfonline.com/doi/full/10.1080/21505594.2024.2395837
H7N9 vaccine in poultry decreased human and avian infections. Analysis of Yangtze River Delta clade E: emergence and peak in wave 5, effective control via vacination, conservation of genetic and antigenic variation
Establishment of a Real-Time Fluorescence Isothermal Recombinase-Aided Amplification Method for the Detection of H9 Avian Influenza Virus
https://www.mdpi.com/2306-7381/11/9/411
Rapid & visual RT–RAA method for detection of H9. No cross-reaction with other influenza viruses. Detection accuracy consistent with RT–qPCR. Results visible to naked eye through a portable blue light instrument.
A Rapid Detection Method for H3 Avian Influenza Viruses Based on RT–RAA
https://www.mdpi.com/2076-2615/14/17/2601
Same approaches and authors as study above.
A case report of human infection with avian influenza H10N3 with a complex respiratory disease history
https://link.springer.com/article/10.1186/s12879-024-09830-y
Another description of the H10N3 human case in China. See: https://www.researchsquare.com/article/rs-4181286/v1
Recombinant Marek’s disease virus type 1 provides full protection against H9N2 influenza A virus in chickens
https://doi.org/10.1016/j.vetmic.2024.110242
Maerks Disease Virus used for recombinant vaccine using H9 influenza. One group showed complete protection against the H9N2 AIV challenge, and also offered complete protection against challenge with MDV = promising bivalent vaccine.
Preparation and Antigenic Site Identification of Monoclonal Antibodies against PB1 Protein of H9N2 Subtype AIV
https://www.mdpi.com/2306-7381/11/9/412
Antigenic determinants in PB1 protein from H9N2. 2 conserved antigenic sites on PB1 protein identified via construction of truncated overlapping fragments. Theoretical reference for H9N2 vaccines?
Contrasting dynamics of two incursions of low pathogenicity avian influenza virus into Australia
https://doi.org/10.1093/ve/veae076
We described the incursion of novel H4 and H10 lineages into Australia, albeit with different patterns. Eurasian lineage H4 found in shorebirds in two subsequent years, but never detected in ducks, which only had Asian lineage H4. H10, in contrast, popped up all over, in waterbirds, but also chickens and implicated in diseases zoo birds.
Evolution and biological characteristics of H11 avian influenza viruses isolated from migratory birds and pigeons
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2398641
Global review of H11 viruses. H11 rare across 35,749 faecal samples collected in China. H11s from ducks replicated better in ducks, those from pigeons replicated better in chickens. some H11 isolates replicated efficiently in mice
First laboratory-confirmed human case of infection with influenza A(H5N2) virus reported in Mexico
https://www.medrxiv.org/content/10.1101/2024.08.15.24311897v1
Potential biosecurity breaches in poultry farms: presence of free-ranging mammals near laying-hen houses assessed through a camera-trap study
https://www.sciencedirect.com/science/article/pii/S2451943X24000607
Camera traps deployed around commercial poultry farms. 7 species of wild mammals (mice, rats, beech marten, red fox, european hare, european hedgehog, coypu) + pets . Coypu + cats = most frequent.
Infection of South American coatis (Nasua nasua) with highly pathogenic avian influenza H5N1 virus displaying mammalian adaptive mutations
https://doi.org/10.1016/j.micpath.2024.106895
HPAI detected in population of 23 coatis in an ecological park, Uruguay, 2023. 5 survived, 4 developed antibodies. Genomes closely associated w backyard poultry. 2 genomes show mammalian adaptation.
Detection and spread of high pathogenicity avian influenza virus H5N1 in the Antarctic Region
https://www.nature.com/articles/s41467-024-51490-8
Detailed analysis of first HPAI sequences from Falklands and S. Georgia. Two seperate introductions from S. America, followed by local transmission. South American Marine mammal clade D701N only found in 1 Kelp Gull and index Fulmars from S. America – not widespread.
Opportunities and challenges for the U.S. laboratory response to highly pathogenic avian influenza A(H5N1)
https://www.sciencedirect.com/science/article/pii/S1386653224000854
Summary of HPAI testing in human labs in the USA – no commercially available test does H5 subtyping. Antigen tests only about 60% sensitive. Potentially some interesting tit bits in here.
Complex Evolutionary Dynamics of H5N8 Influenza A Viruses Revealed by Comprehensive Reassortment Analysis
https://www.mdpi.com/1999-4915/16/9/1405
Reassortment prolific in HPAI H5N8.
Experimental infection of Chickens, Pekin ducks, Eurasian wigeons and Barnacle geese with two recent highly pathogenic avian influenza H5N1 clade 2.3.4.4b viruses
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2399970
Comparison of two 2344 genotypes in 3 different species. Differences in pathogenicity, neurotropism, mortality, but similar shedding. Subclinical infections in Pekin ducks + Eurasian wigeons. All key traits for efficient spread in wild bird population.
Introducing a framework for within-host dynamics and mutations modelling of H5N1 influenza infection in humans
https://www.medrxiv.org/content/10.1101/2024.09.01.24312235v1
Model for within human spread of HPAI. Fitted based on human pharangeal swab. Probability of 3 mutations required for human droplet spread to occur within humans: 10−3. Includes consideration of both lower and upper resp tract.
Highly pathogenic avian influenza H5 virus exposure in goats and sheep
https://www.biorxiv.org/content/10.1101/2024.08.31.610397v1
Serosurvey of goats and sheep in Pakistan: H5 (23.9–34.0%), H7 (13.9– 37.1%), and H9 (17.0–34.7%) > Spill over of avian influenza from poultry into other livestock.
Farmed fur animals harbour viruses with zoonotic spillover potential
https://www.nature.com/articles/s41586-024-07901-3
Three subtypes of influenza A virus—H1N2, H5N6 and H6N2—were detected in the lungs of guinea pig, mink and muskrat, respectively
Phylodynamics of high pathogenicity avian influenza virus in Bangladesh identifying domestic ducks as the amplifying host reservoir
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2399268#d1e365
Domestic ducks are the key reservoir for HPAI in Bangladesh, with occasional spill over into other hosts, including House Crows, which have annual epidemics.
Avian Influenza H5N1 threatens imperiled krill-dependent predators in Antarctica
https://www.frontiersin.org/journals/marine-science/articles/10.3389/fmars.2024.1453737/abstract
No article yet. Provisionally accepted.
Location, Age, and Antibodies Predict Avian Influenza Virus Shedding in Ring-Billed and Franklin’s Gulls in Minnesota
https://www.preprints.org/manuscript/202408.1524/v1
Franklins and Ring-billed Gulls clearly important hosts for influenza viruses, with high prevalence and seroprevalence in these species. Aligns with Black-headed Gulls in Europe. Should not be overlooked in surveillance programs.
Decoding non-human mammalian adaptive signatures of 2.3.4.4b H5N1 to assess its human adaptive potential
https://www.biorxiv.org/content/10.1101/2024.08.26.609722v1
Exposure and Survival of Wild Raptors During the 2022-2023 Highly Pathogenic Influenza A Virus Outbreak
https://www.researchsquare.com/article/rs-4759859/v1
Do raptors survive HPAI infection? Do raptors survive HPAI infection? – Yes! Serology from rehab centres: Bald eagles: IAV seroprev = 69.1% (67/97), and 77.6% (52/67) positive for antibodies to both H5 and N1! Antibodies found in 6 species. Obvious bias here to survived birds, but encouraging news.
Clade 2.3.4.4b but not historical clade 1 HA replicating RNA vaccine protects against bovine H5N1 challenge
https://www.researchsquare.com/article/rs-4946897/v1
Pandemic vaccine stockpiles generally use old antigen (Vietnam/2004), which confer only partial protection against 2344. Replicating RNA vaccine expressing HA of an H5N1 isolated from a US dairy cow confers complete protection against homologous lethal challenge in mice. = Nucleic acid vaccines useful for rapid updates.
Promising effects of duck vaccination against highly pathogenic avian influenza, France 2023-24
https://www.biorxiv.org/content/10.1101/2024.08.28.609837v1
France vaccinating domestic ducks against HPAI. Predicted number of outbreaks (without vaccination) was significantly higher than the observed cases following vaccination, = 95.9% reduction in outbreaks attributable to vaccination. (Ducks much harder to vaccinate than poultry – need more doses, so incredible that this worked so well)
Highly Pathogenic Avian Influenza A Virus in Wild Migratory Birds, Qinghai Lake, China, 2022
https://wwwnc.cdc.gov/eid/article/30/10/24-0460_article
First outbreak of HPAI H5N1 in wild birds occured in Lake Qinghai in 2005. Here, outbreak with 2344b in 2022 – segs most similar to H5N1 in birds in Asia. 2344b was not detected in 2023 – no local maintenance in L. Qinghai.
Highly Pathogenic Avian Influenza Virus H5N1 clade 2.3.4.4b in Wild Rats in Egypt during 2023
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2396874#d1e261
Not just mice in dairy barns: HPAI H5N1 in wild rats collected from a rural area in Giza, Egypt, near poultry farms, markets, and backyard flocks.
Intranasal administration of octavalent next-generation influenza vaccine elicits protective immune responses against seasonal and pre-pandemic viruses
https://journals.asm.org/doi/10.1128/jvi.00354-24
Development of an octavalent (with seasonal + pre-pandemic subtypes) computationally optimised human influenza vaccine: antibodies elicited in ferrets, ferrets survived lethal H5N1 Viet/04 challenge infection.
Déjà Vu All Over Again — Refusing to Learn the Lessons of Covid-19
https://www.nejm.org/doi/full/10.1056/NEJMp2406427
Feature of this article in the Guardian:
US repeating Covid mistakes with bird flu as spread raises alarm, experts say
https://www.theguardian.com/us-news/article/2024/aug/30/bird-flu-covid?utm_source=dlvr.it&utm_medium=twitter
Surveillance of avian influenza viruses in Hebei Province of China from 2021 to 2023: identification of a novel reassortant H3N3
https://www.sciencedirect.com/science/article/pii/S0163445324001749
Screened 6,000+ environmental samples (mostly faeces) in Northern China. 10 isolates, mostly H5N, H7, H9. But an interesting H3N3 isolate. AIVs closely related to previously identified viruses in Yellow River Basin.
Bats from the Colombian Caribbean Reveal a new subtype of Influenza A (H18N12)
https://www.biorxiv.org/content/10.1101/2024.08.25.609627v1
Bat influenza viruses are wild, and here the authors propose a new NA subtype. However, looking at the phylogeny, I’m not sure the node is deep enough to call it a new subtype. 81% similar to N11 at the nt level. No doubt there is undiscovered diversity out there.
PB1-F2 of low pathogenicity H7N7 restricts apoptosis in avian cells
https://www.sciencedirect.com/science/article/pii/S0168170224001370
What is the role of PB1-F2 in AIV? Avian is full length, truncated in mammals. Here, full-length PB1-F2 of LPAIV prolonged survival of infected cells by limiting apoptotic cell death = prolonged infections without severely harming the avian host
Avian influenza viruses in wild birds in Canada following incursions of highly pathogenic H5N1 virus from Eurasia in 2021-2022
https://journals.asm.org/doi/10.1128/mbio.03203-23
Great overview of Canada’s interagency surveillance program. 6,246 sick/dead wild birds tested, 27.4% HPAIV +ve, across 12 avian orders/80 species! 11,295 “healthy” wild birds, 5.2% HPAIV positive, 3 avian orders/19 species.
“highlights a need for sustained investment in wild bird surveillance and collaboration across interagency partners”
Highly pathogenic avian influenza A virus subtype H5N1 (clade 2.3.4.4b) isolated from a natural protected area in Peru
https://journals.asm.org/doi/full/10.1128/mra.00417-24
HPAI H5N1 in Sanderling in Peru. Sanderlings are often not positive for LPAI, and we still have little understanding of impact of HPAI on shorebirds, globally.
High pathogenicity avian influenza in Australia and beyond: could avian influenza cause the next human pandemic?
https://doi.org/10.1071/MA24040
Overview of avian influenza situation, with a focus on Australia
A broad-spectrum vaccine candidate against H5 viruses bearing different sub-clade 2.3.4.4 HA genes
https://www.nature.com/articles/s41541-024-00947-4
Characterization of antigenicity of 2344 HPAI = identification of promising vaccine candidate positioned centrally in the antigenic map. Vaccine = 100% protection of chickens against antigenically drifted H5 viruses from various 2.3.4.4 antigenic groups. = broad spectrum + future proof vaccines
Enhanced Diversifying Selection on Polymerase Genes in H5N1 Clade 2.3.4.4b: A Key Driver of Altered Species Tropism and Host Range Expansion
https://www.biorxiv.org/content/10.1101/2024.08.19.606826v1
Interrogation of select on HPAI H5N1. PB2, PB1, PA = ???? selection pressures in 2.3.4.4b than earlier H5N1 clades. Selection facilitated expanded host tropism + potential for adaptation to mammalian hosts. Paper also has list of interesting mutations found in mammals to date (Fig 3)
Large-Scale Computational Modeling of H5 Influenza Variants Against HA1-Neutralizing Antibodies
https://www.biorxiv.org/content/10.1101/2024.07.14.603367v3
Association between highly pathogenic avian influenza outbreaks and weather conditions in Japan
https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_23-0521/_article/-char/ja/
In Japan, HPAI outbreaks particularly common in autumn + winter, w 2022/23 winter worst. = specific weather conditions associated w ???? outbreaks on poultry farms. Higher average air temperatures 2-3 weeks prior, lower average wind speeds 4 weeks prior, & longer sunlight hours 2 & 4 weeks prior to outbreaks were significantly associated.
Development of a dual-component biosensor for rapid and sensitive detection of influenza H7 and H5 subtypes
https://doi.org/10.1016/j.talanta.2024.126704
Development a novel dual-component biosensor assembly, each component of which incorporates an antibody fused with a nano-luciferase subunit. applied the biosensor in paper-based assay and lateral flow assay formats = field application.
Enhancing wastewater testing for H5N1 surveillance
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00503-6/fulltext
Response to editorial: https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00439-0/fulltext
Overall – utilising wastewater systems can be useful in a scenario where farmers dont come forward for voluntary testing of animals.
Pre-existing H1N1 immunity reduces severe disease with cattle H5N1 influenza virus
https://www.researchsquare.com/article/rs-4935162/v1
Pre-existing immunity from the 2009 H1N1 pandemic provided protection from mortality and severe clinical disease to ferrets intranasally infected with bovine H5N1. Different tissue tropism in H1N1 immune ferrets.
Surveillance for respiratory viruses in freshwater bodies visited by migratory birds, the Philippines
https://ojs.wpro.who.int/ojs/index.php/wpsar/article/view/1123
Environmental water from bird sanctuaries, riverbanks, creeks, marshlands, irrigation canals and rice fields in Philippines + screened for select human viruses. Detection of 1 H9.
Sequence analysis and molecular characterization of low pathogenic avian influenza H9N2 virus isolated from chickens in Sabah
https://msptm.org/files/Vol41No2/tb-41-2-008-Shohaimi-S-A.pdf
H9N2 endemic in Malaysia, but outbreak w high mortality observed. Analysis shows clade h9.4.2.5 in the Y280 lineage, but different clade compared to what is usually found in Malaysia = recent introduction.
Next-generation sequencing technology reveals the viruses carried by poultry in the live poultry market of Guangdong, China
https://www.sciencedirect.com/science/article/pii/S0378113524001585?via%3Dihub
Metatranscriptomic study of samples collected from live poultry markets in China. Picornaviridae, Retroviridae, Coronaviridae, and Othomyxoviridae -> H9N2 detected..
Unsustainable production patterns and disease emergence: The paradigmatic case of Highly Pathogenic Avian Influenza H5N1
https://www.sciencedirect.com/science/article/pii/S0048969724055396
Review of how intensive food production systems are contributing to emergence of pathogens like HPAI H5N1. Transformative actions are required to reduce the emergence and impact of dangerous pathogens
Viral shedding and environmental dispersion of two clade 2.3.4.4b H5 high pathogenicity avian influenza viruses in experimentally infected mule ducks: implications for environmental sampling
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-024-01357-z
Detailed outcomes of experimental infections of 2016/17 and 2020/21 HPAI viruses in Mule ducks. Shedding started 1dpi, before clinical signs. viral RNA in aerosols, dust, and water samples mirrored viral shedding dynamics. Important for duck farms
The panzootic spread of highly pathogenic avian influenza H5N1 sublineage 2.3.4.4b: a critical appraisal of One Health preparedness and prevention
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00438-9/abstract
Overview of HPAI in a OneHealth context, with commentary on weaknesses in pandemic preparedness and prevention as well as steps forward. Authored by the One Health High-Level Expert Panel
Descriptive epidemiology and phylogenetic analysis of highly pathogenic avian influenza H5N1 clade 2.3.4.4b in British Columbia (B.C.) and the Yukon, Canada, September 2022 to June 2023
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2392667
Comparing HPAI between 1st and 2nd wave in W Canada. Wave 2: more outbreaks, but tightly clustered, different species affected, 7 genotypes + 2 incursions. Many spillovers from wildbirds to poultry + mammals.
Genome sequences of haemagglutinin cleavage site predict the pathogenicity phenotype of avian influenza virus: statistically validated data for facilitating rapid declarations and reducing reliance on in vivo testing
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2317430
Old paper I seem to have missed..Data provides statistical support to continued use of molecular determination of pathotype for AI viruses based on HA cleavage site sequence in absence of an in vivo study determination. Expedites declaration process of HPAIV & reduces need for experimental in vivo testing of H5 & H7 viruses.
Comparative examination of a rapid immunocytochemical test for the detection of highly pathogenic avian influenza virus in domestic birds in field outbreaks
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2320699
AIV antigen detection examined in field outbreaks. Bird brain smears tested using immunocytochemistry (IC). IC results strongly correlated with real-time RT-PCR results. IC method rapid, specific, sensitive, & cost-effective in AIV field outbreaks.
Characterization of Avian Influenza Viruses Detected in Kenyan Live Bird Markets and Wild Bird Habitats Reveal Genetically Diverse Subtypes and High Proportion of A(H9N2), 2018-2020
https://www.preprints.org/manuscript/202408.0600/v1
Surveillance for AIV in Kenya amongst poultry, wild birds. H9 found in poultry and similar to viruses in E. Africa. In wild birds, LPAI H5 Eurasian lineage and H11. Poultry workers with ARI all negative.
Serological survey of high and low pathogenic avian influenza viruses in migratory waterbirds of Neor Lake, Ardabil, northwest of Iran
https://jzd.tabrizu.ac.ir/article_18310.html
Tested blood samples tested H5, H7 and H9 subtypes using HI. Sera negative for H5N1, H5N2, H7N1 & H7N7 viruses. 11.89% birds seropositive for H9N2 virus. Mallards = highest seroprevalence (25 %) & whooper swan = lowest (10.5 %).
A Review of the Stability of Avian Influenza Virus in Materials from Poultry Farms
https://doi.org/10.1637/aviandiseases-D-23-00027
Review on the stability of AIV in materials from poultry farms that cannot be disinfected with chemicals or fumigants: water, litter/bedding, soil, feed, feathers, carcasses/meat, manure/feces, and eggs. Could not access pdf.
Viral Shedding Evaluation is Critical for Determining Efficacy of Avian Influenza (H9) Vaccines in Broiler Chickens
https://researcherslinks.com/current-issues/Viral-Shedding-Evaluation-is-Critical/20/1/8402/html
Evaluated comparative efficacy of commercial & self-prepared AIV H9 vaccines for potential to stop or reduce viral shedding post challenge infection & to develop humoral immunity. All vaccines found effective reducing virus shedding & induction of humoral immunity. None stopped shedding. .
Global antigenic landscape and vaccine recommendation strategy for low pathogenic avian influenza A (H9N2) viruses
https://www.journalofinfection.com/article/S0163-4453(24)00133-6/fulltext
Developed “PREDAC-H9” method to map global antigenic landscape of H9N2 AIVs and predict antigenic relationship between any two viruses with high accuracy (>80%). 10 major antigenic clusters identified in H9N2 AIVs from 1966-2022. 4 novel clusters generated in China in past decade.
Passive immunisation of mice with IgY anti-H5N1 protects against experimental influenza virus infection and allows development of protective immunity
https://www.sciencedirect.com/science/article/pii/S0264410X24007965
Giving chicken IgY anti-H5N1 to mice protects them from disease and mice developed influenza virus-specific memory T cells similar to control-treated mice. = Passive immunization used as prophylactic in combo w immunization to prevent disease in mice.
Exploring surface water as a transmission medium of avian influenza viruses – systematic infection studies in mallards
https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2065937
Water is important for transmission of AIV. Here, shown experimentally by the FLI crew through three different experiments: surface water in small shallow water bodies may play an important role as a mediator of LPAI and HPAI.
Natural and Experimental Persistence of Highly Pathogenic H5 Influenza Viruses in Slurry of Domestic Ducks, with or without Lime Treatment
https://journals.asm.org/doi/10.1128/aem.02288-20
Evaluation of HPAI survival in effluent from duck farms in France. Without lime treatment, virus survived for 4 weeks in slurry from Muscovy or Pekin duck breeders & for 2 weeks in slurry from ducks for foie gras production during assisted-feeding period. = Experimental support for 60-day storage period w/out treatment or 7-day interval after lime treatment defined in French regulations for slurry sanitization.
High pathogenicity avian influenza (HPAI) in Great Britain and Europe
https://assets.publishing.service.gov.uk/media/66a3c05bab418ab055592e37/HPAI_Europe__1_17_July_2024.pdf
Situation assessment of HPAI in Great Britain and Europe. Since last outbreak assessment on 1 April 2024, no reports of HPAI H5 clade 2.3.3.4b in domestic poultry in Great Britain but 2 HPAI H5 clade 2.3.3.4b events involving “found-dead” wild birds, although one was retrospectively tested from a sample collected last year.
Emergence of a human co-infected with seasonal influenza A (H3N2) virus and avian influenza A (H10N5) virus, China, December 2023
https://www.sciencedirect.com/science/article/pii/S1684118224001191
Journal publication of a WHO and China CDC report from before.
https://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2024.106
Death of 63-year-old woman in Anhui Province, China, due to co-infection with seasonal IAV (H3N2) subtype virus & AIV A (H10N5) subtype virus on January 27, 2024. First reported human infection with H10N5. Clinical presentation = flu like symptoms. Exposure to duck meat on 26 November 2023 = 7 samples found to be + for H10N5 & 2 samples + for N5.
Recombinant Hemagglutinin Protein from H9N2 Avian Influenza Virus Exerts Good Immune Effects in Mice
https://www.mdpi.com/2076-2607/12/8/1552
Mice given recombinant HA protein. In response, mice produced IgG antibodies, promoted secretion of cytokines & reduced accumulation of some inflammatory factors, which may involve different methods of protecting host. No challenge studies.
An overview of avian influenza surveillance strategies and modes
https://www.sciencedirect.com/science/article/pii/S2949704323000379
Collates & examines features & experiences of global, regional, & national AIV surveillance efforts in context of One Health.
Machine learning approaches for influenza A virus risk assessment identifies predictive correlates using ferret model in vivo data
https://www.nature.com/articles/s42003-024-06629-0
Never need to do ferret trials again? Data from 125 contemporary IAV (H1, H2, H3, H5, H7, & H9 subtypes) in ferrets fed into machine learning to show models can summarise lethality, morbidity, transmissibility.
Waterfowl abundance during the 2022-2023 high pathogenicity avian influenza epidemic in Iowa
https://ehp.niehs.nih.gov/doi/abs/10.1289/isee.2024.1556
Conference abstract only. Seems like there is an association between Canada Goose abundance and HPAI outbreaks in Iowa – Mallards perhaps not to blame?.
Impacts and lessons learned from the first highly pathogenic avian influenza (H5N1) outbreak in South American pinnipeds along the southern Brazilian coast
https://onlinelibrary.wiley.com/doi/full/10.1111/mms.13163
Detailed outline of Brasilian response to HPAI in marine mammals. Surveys found 271 sea lions + 217 fur seals b/w sept – dec 2024 w 82.6% dead, 10.9% alive w HPAI disease, 6.6% w no apparent symptoms while alive. Lessons can be learned. .
Effects of H9N2 avian influenza virus infection on metabolite content and gene expression in chick DF1 cells
https://www.sciencedirect.com/science/article/pii/S0032579124007041
Impact of AIV on metabolites & gene expression in poultry cells previously unclear. Infected chicken embryo fibroblasts DF1 cells with H9N2 AIV. Infection activated glutathione metabolic pathway to enhance cell’s self-defence mechanism..
Prevalence of Avian Influenza Virus in Atypical Wild Birds Host Groups during an Outbreak of Highly Pathogenic Strain EA/AM H5N1
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/4009552
Results from passive surveillance system in USA. Columbiformes & Passeriformes appear to hold less risk of AIV infection compared to wild predatory & scavenging orders, in which the most prevalent AIV detections were found. Consumption of infected tissues = key pathway for transmission of AIV in predatory & scavenging birds.
Characterization and Pathogenicity of Novel Reassortment H6N6 Avian Influenza Viruses in Southern China
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/4005909
2 different genotypes of H6N6 in ducks in China. Receptor-binding preference & pathogenicity evlauted in poultry & mice. One viruscan bind to α-2,6 & α-2,3 receptors, the other only avian-origin α-2,3 receptors.
Investigating whether H5N1 is a risk to human populations in Brazil
https://www.scielo.br/j/rsbmt/a/tbq4CMVzyRZDhHPxT4V4cGr/?lang=en
Raises concerns about H5N1 risk to human population in Brazil & proposes strategies to mitigate associated public health risks.
Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
https://www.biorxiv.org/content/10.1101/2024.05.23.595634v2
Has already been reviewed, but I’ve included it again because it has been updated since its first release.
Use pseudovirus deep mutational scanning to measure how all mutations to a clade 2.3.4.4b H5 HA affect each phenotype. Identify mutations that allow HA to better bind α2-6-linked sialic acids & show that some viruses already carry mutations that stabilise HA. Identify recent viral strains with reduced neutralisation to sera elicited by candidate vaccine virus.
Tracking the spread of avian influenza A(H5N1) with alternative surveillance methods: the example of wastewater data
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00498-5/fulltext
Integration of wastewater into the Branda dataset..
Ecology and evolution of avian influenza viruses
https://www.sciencedirect.com/science/article/pii/S0960982224006961
Overview of ecology and evolution of AIV by Geroge Gao et al
Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza
https://www.nature.com/articles/s41541-024-00932-x
Report on immunogenicity & biodistribution of four H5 HA-based self-amplifying (sa) mRNA vaccines in mice. All vaccines elicited adaptive immune response. Full-length HA sa-RNA vaccines demonstrated superior performance compared to head & stalk domain vaccines. Antibody titers positively correlated with vaccine dose.
Mass vaccination with reassortment-impaired live H9N2 avian influenza vaccine
https://www.nature.com/articles/s41541-024-00923-y
Live virus vaccines not often used for AIV >> risk of reassortment. Here, reassortment-impaired, non-transmissible live virus H9N2 vaccine: rearrangement of internal segments +modifications to HA and NA. Not 100% protective.
Amplification of avian influenza virus circulation along poultry marketing chains in Bangladesh: a controlled field experiment
https://doi.org/10.1016/j.prevetmed.2024.106302
Previously reviewed as a preprint. Live bird markets are a hotspot for HPAI. New study investigates AIV infections during marketing chains, and with testing during transport/trade, intervention group had lower shedding once arrived at LBM.
Avian Influenza Virus A(H5Nx) and Prepandemic Candidate Vaccines: State of the Art
https://www.mdpi.com/1422-0067/25/15/8550
Review with good summary of current status of enzootics, & challenges for H5 vaccine manufacturing & delivery.
H5N1 influenza: Urgent questions and directions
https://www.cell.com/cell/abstract/S0092-8674(24)00784-0
Cocirculation of Genetically Distinct Highly Pathogenic Avian Influenza H5N5 and H5N1 Viruses in Crows, Hokkaido, Japan
https://wwwnc.cdc.gov/eid/article/30/9/24-0356_article
HPAI H5N5 +H5N1 from crows in Hokkaido, Japan, during winter 2023–24 = shared genetic similarity with HPAI H5N5 from northern Europe but differed from those in Asia. = introduced into Japan through a step-by-step bird migration through northern Eurasia
Effects of adding antibiotics to an inactivated oil-adjuvant avian influenza vaccine on vaccine characteristics and chick health
https://www.sciencedirect.com/science/article/pii/S0032579124007144
During poultry vaccination, antibiotics are typically added to inactivated oil-adjuvant avian influenza vaccines. Adding ceftiofur = ????chick growth and gut microbiota modulation, disrupt vaccine structure, ???? vaccine safety + efficacy
A vaccine antigen central in influenza A(H5) virus antigenic space confers subtype-wide immunity
https://www.biorxiv.org/content/10.1101/2024.08.06.606696v1
How to make good H5 vaccines w broad coverage? Here, antigen map developed, used to make immunogenic + antigenically central vaccine HA antigens, eliciting antibody responses that broadly cover the H5 antigenic space. Vaccines = good coverage in ferrets.
Recent global outbreaks of highly pathogenic and low-pathogenicity avian influenza A virus infections
https://www.tandfonline.com/doi/full/10.1080/21505594.2024.2383478
Review on recent cases of human HPAI A(H5N1), HPAI A(H7N9), LPAI A(H9N2), LPAI A(H10N3) & A(H5N2) virus infections and One Health approach to monitor and control HPAI and LPAI.
High and low pathogenicity avian influenza virus discrimination and prediction based on volatile organic compounds signature by SIFT-MS: a proof-of-concept study
https://www.nature.com/articles/s41598-024-67219-y
They used volatile organic compounds signatures to discriminate HPAIV & LPAIV infected cells from control cells. Fed all this into a complex model to predict whether cells infected with what virus. Unclear why this would be useful at this stage – perhaps the start of a novel detection method?
Unusual A(H1N7) influenza A virus isolated from free-range domestic ducks in Bangladesh, 2023
https://journals.asm.org/doi/full/10.1128/mra.00218-24
Diversity of H1N7 viruses from domestic ducks in Bangladesh with genomes comprising segments closely related to both European and Asian viruses..
Mortality of H5N1 human infections might be due to H5N1 virus pneumonia and could decrease by switching receptor
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00460-2/fulltext
Suggest pneumonia cause of high CFR for H5N. Hypothesis: if H5N1 switches to upper airway receptor (SA α2,6), mortality would be lower because infections would be restricted to upper respiratory tract infections.
Modelling molecular differences in the innate immune system responses of chickens and ducks to highly pathogenic avian influenza virus
https://www.biorxiv.org/content/10.1101/2024.07.26.605270v1.abstract
Against HPAI: chickens produce a pro-inflammatory response; mallards produce an anti-viral response. an avian innate immune response agent-based model developed, confirming key role of RIG-I.
Effectively Evaluating a Novel Consensus Subunit Vaccine Candidate to Prevent the H9N2 Avian Influenza Virus
https://www.mdpi.com/2076-393X/12/8/849
Bioinformatically derived broad-spectrum H9 protein, cross-reactivity against sera of several subbranch H9, and vaccine candidate provided complete clinical protection but did not prevent shedding in chickens
It’s time to apply outbreak response best practices to avian influenza: A national call to action
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11232087/
Focussed on Canada, recommend veterinarians align with national stakeholders to apply Outbreak Response Best Practices to AHPAI using a formal Quality Management System.
Antibodies to Influenza A Virus in Lesser (Aytha affinis) and Greater Scaup (Aytha marila) in the USA
https://meridian.allenpress.com/jwd/article-abstract/doi/10.7589/JWD-D-24-00021/501872/Antibodies-to-Influenza-A-Virus-in-Lesser-Aytha?redirectedFrom=fulltext
Ducks is not a duck is not a duck. In literature, no virology data, scaup are are seropositive with decent seroprevalence. So what’s up with diving ducks? In USA, H1N1 isolated, and antibodies against H1–H12, H14, and H15 via serology..
Histopathologic Features and Viral Antigen Distribution of H5N1 Highly Pathogenic Avian Influenza Virus Clade 2.3.4.4b from the 2022–2023 Outbreak in Iowa Wild Birds
https://meridian.allenpress.com/avian-diseases/article-abstract/doi/10.1637/aviandiseases-D-23-00085/501918/Histopathologic-Features-and-Viral-Antigen?redirectedFrom=fulltext
Histology of dead raptors + waterfowl infected w HPAI. NP detected commonly in the lungs (77.8%), kidney (75%), heart (66.7%) & liver (55.6%). Pancreas, spleen, intestines, gonads, & adrenals occasionally exhibited + viral protein signals.
The amino acid variation at hemagglutinin sites 145, 153, 164 and 200 modulate antigenicity and replication of H9N2 avian influenza virus
https://doi.org/10.1016/j.vetmic.2024.110188
In H9 viruses, mutation at site 164 significantly modified antigenic characteristics. Amino acid variations at sites 145, 153, 164 and 200 affected virus’s hemagglutination and the growth kinetics in mammalian cells.
A Rationally Designed H5 Hemagglutinin Subunit Vaccine Provides Broad-Spectrum Protection against Various H5Nx highly Pathogenic Avian Influenza Viruses in Chickens
https://www.preprints.org/manuscript/202407.1019/v1
When chickens vaccinated with novel baculovirus-based vaccine at 1 day old, 100% protection against 2.3.4.4b challenge at 3 weeks of age. 100% protection from mortality and clinical signs, and no shedding.
Immunisation of chickens with inactivated and/or infectious H9N2 avian influenza virus leads to differential immune B cell repertoire development
https://www.biorxiv.org/content/10.1101/2024.07.09.602583v1.abstract
Changes in H9N2-specific IgM and IgY in chickens vaccinated or challenged: . ^ proportion of IgM & IgY clones shared across multiple individuals, but dependent on immunisation status & specific tissue examined. Specific clonal expansions restricted to particular H9N2 immunisation regimes. == nature & number of immunisations important drivers of antibody responses & repertoire profiles in chickens following H9N2 antigenic stimulation.
Efficacy of commercial recombinant HVT vaccines against a North American clade 2.3.4.4b H5N1 highly pathogenic avian influenza virus in chickens
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0307100
Tested 2 recombinant herpes virus vaccines (COBRA-HVT and 2.2-HVT vaccine) against HPAI in chickens. High survival in vaccinated birds, and lower cloacal viral shedding from COBRA-HVT. But shedding non-the-less.
Ecology and environment predict spatially stratified risk of highly pathogenic avian influenza in wild birds across Europe
https://www.biorxiv.org/content/10.1101/2024.07.17.603912v1
Model identifying factors driving geospatial distribution of HPAI + project the distribution of risk across Europe, explicitly considering impact of wild bird ecology. == shift in risk towards cold, low-lying regions of coastal northwest Europe. Predict persistence of ^ risk in coastal northwest Europe throughout the year. Majority of variation in risk explained by climate & aspects of physical geography. Addition of ecological covariates represents valuable refinement to species distribution models of HPAI.
Cytomegalovirus vaccine vector-induced effector memory CD4 + T cells protect cynomolgus macaques from lethal aerosolized heterologous avian influenza challenge
https://www.nature.com/articles/s41467-024-50345-6
Immunised Mauritian cynomolgus macaques with cynomolgus CMV vaccines w H1N1 1918 influenza M1, NP, & PB1 antigens and challenged with heterologous, aerosolized avian H5N1 influenza. All six unvaccinated macaques died with acute respiratory distress. 54.5% vaccinated macaques survived. CD4 + T cells targeting conserved internal influenza proteins can protect against HPAI.
Mitigating Risk: Predicting H5N1 Avian Influenza Spread with an Empirical Model of Bird Movement
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/5525298
Model w sat tagged waterfowl + HPAI in poultry: projects exposure & spread of HPAIv among waterfowl, but predictions of HPAIv detections in poultry lagged
Risk of invasive waterfowl interaction with poultry production: Understanding potential for avian pathogen transmission via species distribution models
https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.11647
In Arkansas, Assessed risk of invasive waterfowl-poultry interaction w focus on Egyptian Goose & Mute Swan. % urban land cover most important habitat characteristic. Densities of poultry in waterfowl areas3-5 x times higher than those others. Tip: don’t build poultry production in places that waterfowl like to hangout.
Coastal connectivity of marine predators over the Patagonian Shelf during the highly pathogenic avian influenza outbreak
https://nsojournals.onlinelibrary.wiley.com/doi/10.1111/ecog.07415
Previous reviewed as a preprint, now out.
How did HPAI go from S. Am to Falklands? Extensive connectivity of Black-browed albatrosses, South American fur seals & Magellanic penguins over Patagonian Shelf: Falklands <->S.Am coast w transit times 0.2–70 days, with 84% of animals transiting within 4 days which is the conservative estimate for HPAI infectious period.
Proposal for a Global Classification and Nomenclature System for A/H9 Influenza Viruses
https://wwwnc.cdc.gov/eid/article/30/8/23-1176_article
Practical lineage classification & nomenclature system based on analysis of 10,638 HA sequences. Incorporates phylogenetic relationships & epidemiologic characteristics designed to trace emerging & circulating lineages & clades. Online tool: https://nmdc.cn/influvar/tools/H9aiv
Detection and genomic characterization of an avian influenza virus A/mute swan/Mangystau/1-S24R-2/2024 (H5N1; clade 2.3.4.4b) strain isolated from the lung of a dead swan in Kazakhstan
https://journals.asm.org/doi/10.1128/mra.00260-24
H5N1 clade 2.3.4.4b strain A/mute swan/Mangystau/1-S24R-2/2024 from lung of dead swan found around Lake Karakol (Kazakhstan) during HPAI outbreak in 2024.
Update on Highly Pathogenic Avian Influenza A(H5N1) Virus for Clinicians and Healthcare Centers
https://emergency.cdc.gov/coca/calls/2024/callinfo_071624.asp
Update on current outbreak in U.S & current CDC surveillance & monitoring efforts. Information for clinicians on testing, using antivirals, & infection prevention/ control recommendations.
Australia’s first human case of H5N1 and the current H7 poultry outbreaks: implications for public health and biosecurity measures
https://www.thelancet.com/journals/lanwpc/article/PIIS2666-6065(24)00135-4/fulltext#:~:text=Australia’s%20first%20human%20HPAI%2DH5N1,and%20proactive%20public%20health%20measures.
Strange paper by indian authors with no involvement.
First detection of HPAI in a mammal on the Antarctic peninsula
https://www-ciencia-gob-es.translate.goog/Noticias/2024/Julio/gripe-aviar-antartida.html?_x_tr_sl=es&_x_tr_tl=en&_x_tr_hl=en&_x_tr_pto=wapp
Multiple transatlantic incursions of highly pathogenic avian influenza clade 2.3.4.4b A(H5N5) virus into North America and spillover to mammals
https://www.sciencedirect.com/science/article/pii/S2211124724008088
Important not to forget the other subtypes. In 2023, transatlantic of HPAI H5N5 to N. Am w seabirds. Canadian birds +mammals w PB2-E627K. Ferret: some evidence of spread by direct contact.
Viral metagenomic survey of caspian seals
https://www.biorxiv.org/content/10.1101/2024.07.14.603418v1
Metagenomics of Caspian sealsOrthomyxoviridae least common viral family recovered. Recovered Influenza A (H3) & partial contigs for Influenza B, representing only the second such molecular identification in marine mammals.
Licensed H5N1 vaccines generate cross-neutralizing antibodies against highly pathogenic H5N1 clade 2.3.4.4b influenza virus
https://www.nature.com/articles/s41591-024-03189-y
Stockpiled H5N1 vaccines developed using older strains do generate cross-neutralizing antibodies against circulating HPAI H5N1 clade 2.3.4.4b in humans & may be useful as bridging vaccines until updated H5N1 vaccines available.
Evaluating the Impact of Low-Pathogenicity Avian Influenza H6N1 Outbreaks in United Kingdom and Republic of Ireland Poultry Farms during 2020
https://www.mdpi.com/1999-4915/16/7/1147
While HPAI H5N1 is having the largest impact, when other subypes enter poultry they may cause morbidity and mortlaity. Here, H6N1 in UK in 2020, against backdrop of H5N1. 2million birds, 15 IP Northern Ireland, 13 in Ireland, 5 in Scotland, & 1 in England.
MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses
https://www.nature.com/articles/s41564-024-01771-1
Human H2N2 and avian H2N2 possess dual receptor specificity in cell lines and primary human airway cultures = entry via MHC class II is independent of sialic acid. MHC class II from humans, pigs, ducks, swans, chickens but not bats mediate H2 IAV entry.
The Haemagglutinin Genes of the UK Clade 2.3.4.4b H5N1 Avian Influenza Viruses from 2020 to 2022 Retain Strong Avian Phenotype
https://www.biorxiv.org/content/10.1101/2024.07.09.602706v1
Evaluated comprehensive panel of H5 viruses representing prevalent genotypes from UK outbreaks spanning 2020-2022 for HA functionality. Viruses only bound to avian receptors & exhibited fusion at pH 5.8, above pH range (pH 5.0-5.5) associated with efficient human-to-human transmission. Concludes H5 viruses have low immediate zoonotic threat. Contemporary H5 viruses more thermostable & showed antigenic drift. N236D in HA significant antigenic epitope.
In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system
https://www.biorxiv.org/content/10.1101/2024.07.12.603202v1
Established rapid (8 hour) in vitro one-pot plasmid construction (IVOC) based virus synthesis. Infectious viruses could be synthesized with similar yield to conventional E. coli cloning-based method with high accuracy.
Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) Virus
https://wwwnc.cdc.gov/eid/article/30/8/24-0785_article
Yet another dual-target reverse transcription PCR for H5 subtyping in human clinical samples. Continuous sequence surveillance & updating of primer–probe sets still required to ensure assay accounts for ongoing viral evolution.
Modified transport medium for improving influenza virus detection
https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1399782/full
Developed modified transport medium (MTM) for clinical sample transportation to increase viral detection sensitivity.Some limitations e.g. temperatures <37°C affect cultivation & long incubation time.
Role of miRNA in Highly Pathogenic H5 Avian Influenza Virus Infection: An Emphasis on Cellular and Chicken Models
https://www.mdpi.com/1999-4915/16/7/1102
miRNAs play a significant role in AIV infections, influencing various aspects of the disease process. Review synthesises recent findings on impact of different miRNAs on immune function, viral cytopathogenicity & respiratory viral replication
Exploring Potential Intermediates in the Cross-Species Transmission of Influenza A Virus to Humans
https://www.mdpi.com/1999-4915/16/7/1129
Review investigates potential intermediate hosts in cross-species transmission of IAV to humans, focusing on factors that facilitate zoonotic events. Evaluates roles of various animal hosts (pigs, galliformes, companion animals, minks, marine mammals & others) in spread of IAV to humans.
Air sampling and simultaneous detection of airborne influenza virus via gold nanorod-based plasmonic PCR
https://www.sciencedirect.com/science/article/abs/pii/S030438942401759X
Crazy new tech – air sampling and simultaneous detection of airborne influenza virus via plasmonic PCR -> plasmonic thermocycling using laser pulse and duration to rapidly heat and cool a solution. Lets see the reality.
Construction of a monoclonal molecular imprinted sensor with high affinity for specific recognition of influenza a virus subtype
https://www.sciencedirect.com/science/article/abs/pii/S0039914024009470
Another out of the box approach. monoclonal molecular imprinted polymers (MIPs) sensor for recognition of H5N1 to permit the accurate distinguishing of H5N1 from other influenza A virus subtype. Can apparently distinguish H5N1 from H1N1, H7N9 and H9N2
Optical transmission for precise discrimination of influenza A virus subtypes: a comprehensive study on light interaction and collimated transmittance
https://link.springer.com/article/10.1007/s12596-024-02013-7
Another out of the box approach. Optical experiment designed specifically to differentiate various influenza virus subtypes. Successfully identified H1N1, H5N1 & H9N2. Each subtype exhibited unique collimated transmission pattern at same incident laser wavelength. Sensitivity could reach 100% at low viral concentrations, at certain wavelengths. Beware – done by non-experts – “breeds of avian influenza”
Modeling transmission of avian influenza viruses at the human-animal-environment interface in Cuba
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1415559/full
Integrated One Health model to estimate likelihood of AIV introduction & transmission in Cuba. South-western & eastern regions of Cuba = ^ risk of transmission & should be targeted to strengthen biosecurity & early warning surveillance.
Biological lags and market dynamics in vertically coordinated food supply chains: HPAI impacts on U.S. egg prices
https://www.sciencedirect.com/science/article/pii/S0306919224000666
Hedonic model of retail egg prices that controls for quality, regional, and temporal factors to reveal role of HPAI. HPAI caused weekly retail egg prices ????5.3 %. importance of understanding context-specific outcomes for agri-food supply chain.
Avian influenza overview March–June 2024
https://www.ecdc.europa.eu/sites/default/files/documents/AI-Report-XXVIX.pdf
Identification of sialic acid receptors for influenza A virus in snakes
https://pubmed.ncbi.nlm.nih.gov/38968671/
Snakes on a plane! Turns out that both SA α2,3-Gal & SA α2,6-Gal receptors can be found in respiratory and digestive tracts of snakes (via lectin immunohistochemistry, not AI prediction). = susceptibility to IAV?.
Epidemiology, biosafety, and biosecurity of Avian Influenza: Insights from the East Mediterranean region
https://pubmed.ncbi.nlm.nih.gov/38886173/
Literature review of AIV focusing on impact in Middle East including virus structure & subtypes, mechanism of infection, disease symptoms & global outbreak history & status.
Financial impacts of a housing order on commercial free range egg layers in response to highly pathogenic avian influenza
https://pubmed.ncbi.nlm.nih.gov/38714017/
Housing orders are an important tool in avian influenza control in poultry. In commercial free-range egg layers: feed use + feed costs per bird increased. But, ???? revenue maybe due to a higher proportion of large eggs produced..
miR-214-PTEN pathway is a potential mechanism for stress-induced immunosuppression affecting chicken immune response to avian influenza virus vaccine
https://pubmed.ncbi.nlm.nih.gov/38692133/
Stress-induced immunosuppression = common problems in intensive poultry industry affecting effect of vaccine = ???? incidence of disease. MiR-214 identified as molecular marker, MiR-214-PTEN = key regulatory mechanism.
High Prevalence of Highly Pathogenic Avian Influenza A Virus in Vietnam’s Live Bird Markets
https://academic.oup.com/ofid/advance-article/doi/10.1093/ofid/ofae355/7700791?login=false
From Jan 2019 – April 2021 OneHealth influenza surveillance @ live bird markets + swine farms in N. Vietnam. In LBMs, lots of influenza everywhere, including human case w H9N2 PB1 gene. Prev low on swine farms. LBMs high risk environments.
Spatiotemporal and Species-Crossing Transmission Dynamics of Subclade 2.3.4.4b H5Nx HPAIVs
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/2862053
Another iteration of the Xie et al Science paper. Nothing substantially new. Phylodynamics of 2344b. HA gene diverged into 2 dominant clusters around 2015 and 2016. Wild Anseriformes primary species contributing to the spatial expansion and rapid diffusion globally
Novel Genotypes of Highly Pathogenic Avian Influenza H5N1 Clade 2.3.4.4b Viruses, Germany, November 2023
https://wwwnc.cdc.gov/eid/article/30/8/24-0103_article
In Germany, in 2023, genotype BB [reassortant w gull-H13], dominated HPAI outbreaks in colony breeding birds. Uptick in cases in late 2023 – 4 new genotypes with new segments donated from LPAI. Surveillance for LPAI and HPAI needed. Changing HPAI dynamics underfoot…
Spatiotemporal patterns of low and highly pathogenic avian influenza virus prevalence in murres in Canada from 2007 to 2022—a case study for wildlife viral monitoring
https://www.facetsjournal.com/doi/full/10.1139/facets-2023-0185
Murres/Guillemots hosts for LPAI in our early work. Here, review shows HPAIV in 46% live/harvested + dead murres in NW Atlantic in 2022, w prevalence at 63% among live birds in the summer! In eastern Canadian Arctic = 21%. Huge impacts.
CRISPR/Cas13a-based genome editing for establishing the detection method of H9N2 subtype avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0032579124006473
H9N2 endemic in poultry. New method for detection of H9N2 based on fluorescence intensity using CRISPR/Cas13a
Genotypic and phenotypic susceptibility of emerging avian influenza A viruses to neuraminidase and cap-dependent endonuclease inhibitors
https://www.sciencedirect.com/science/article/pii/S0032579124006473
Resistance to antiviral drugs low in >20,000 avian influenza tested; all tested subtypes were susceptible to NAIs and baloxavir at sub-nanomolar concentrations.
Quantitative Risk Assessment of Wind-Supported Transmission of Highly Pathogenic Avian Influenza Virus to Dutch Poultry Farms via Fecal Particles from Infected Wild Birds in the Environment
https://www.mdpi.com/2076-0817/13/7/571
Model to determine probability that aerosolization of fecal droppings from wild birds in the vicinity of poultry farms = infection of indoor-housed poultry w HPAI. Happens once ever 455 years = unlikely event (or model is no good?)
Changing epidemiological patterns in human avian influenza virus infections
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(24)00158-7/fulltext
The story of human infections with HPAI continues to change. Lots for us to learn about human infections, better diagnostics (e.g. physician education to recognise potential cases), vaccinating high risk people can be considered. Lots of interesting points raised.
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination
https://www.sciencedirect.com/science/article/pii/S104659282400113X?via%3Dihub
Full length HA protein from H5N1 strain successfully cloned, expressed, & purified in E. coli. Potential target for future vaccine development.
Identification of broad-spectrum B-cell and T-cell epitopes of H9 subtype avian influenza virus HA protein using polypeptide scanning
https://www.sciencedirect.com/science/article/pii/S209531192400248X
Based on the phylogenetic and serological analyses, 2 antigenic groups of H9N2. Identified 5 novel cell epitopes that could be targeted for vaccine design or detection approaches against H9N2 AIVs.
An emerging PB2-627 polymorphism increases the pandemic potential of avian influenza virus by breaking through ANP32 host restriction in mammalian and avian hosts
https://www.biorxiv.org/content/10.1101/2024.07.03.601996v2.abstract
Common mammalian mutation PB2-E627K previously not maintained in AIV in poultry. New PB2-627V mutation facilitates AIVs to efficiently infect & replicate in chickens & mice utilizing avian & human origin ANP32A proteins. Promotes efficient transmission between ferrets through respiratory droplets & remains stable across distinct hosts. ^ potential for long-term prevalence in avian species. Important to monitor AIV carrying PB2-627V to prevent pandemic.
Clustering broiler farmers based on their behavioural differences towards biosecurity to prevent highly pathogenic avian influenza
https://www.sciencedirect.com/science/article/pii/S2352771424001782
Compared relationships among farmers’ biosecurity behaviours, risk of HPAI infection & features of commercial broiler farmers across 303 farms. Found distinct features based on social backgrounds and production types.
Mortality in sea lions is associated with the introduction of the H5N1 clade 2.3.4.4b virus in Brazil October 2023: whole genome sequencing and phylogenetic analysis
https://link.springer.com/article/10.1186/s12917-024-04137-1
SeaLion story continues, now in Brazil. Lots more dead sea lions. Genotype B3.2 of clade 2.3.4.4b HPAI H5N1 first detected in South American sea lions in Brazil October 2023. Acquired new amino acid substitutions related to mammalian host affinity.
Simultaneous differential detection of H5, H7 and H9 subtypes of avian influenza viruses by a triplex fluorescence loop-mediated isothermal amplification assay
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1419312/full
Novel triplex LAMP assay developed to simultaneously detect H5, H7, H9 AIV subtypes. Detection limit = 205 copies per reaction for H5, 360 copies for H7, & 545 copies for H9. Good specificity, no cross-reactivity with related avian viruses & 100% consistency with previously published qPCR assay.
CD8+T Cell Epitope Conservation in Emerging H5N1 Viruses Suggests Global Protection
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4890883
>64% of CD8+ T cell epitopes highly conserved in H5N1 viruses, with 60% of most prevalent HLA-I w at least one immunogenic CD8+ T cell epitope conserved in H5N1 viruses. T cell cross-recognition against H5N1 should provide some protection in humans
Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) Virus
https://wwwnc.cdc.gov/eid/article/30/8/24-0785_article
Updated H5 qPCR primers/probes. Could be used to detect influenza A(H5) in clinical samples. Continuous sequence surveillance & updating of primer–probe sets still required to address ongoing viral evolution.
Evaluating the epizootic and zoonotic threat of an H7N9 low-pathogenicity avian influenza virus (LPAIV) variant associated with enhanced pathogenicity in turkeys
https://doi.org/10.1099/jgv.0.002008
Another banger from Joe James. From 2013-2017 >1000 human cases of H7N9, halted due to vaccination. New Q217 mutation in turkey H7N9 viruses. H7N9 infection in turkeys = novel variants w ????risk via pathogenicity & HA antigenic escape. Turkey virus replicated in ferrets.
Preventive, safety and control measures against Avian Influenza A(H5N1) in occupationally exposed groups: A scoping review
https://www.sciencedirect.com/science/article/pii/S2352771424000922
How to better protect occupationally exposed workers to HPAI? Biosecurity (PPE, handwashing) = crucial prevention measure for exposed workers. Workers ^ receptive to information from media (TV, radio) & expert opinion.
First sighting of human H5N1 in Australia: A detailed account and public health implication
https://www.sciencedirect.com/science/article/pii/S2052297524002312
1st human H5N1 case in Australia highlights need for vigilant surveillance, rapid response systems, & international cooperation to mitigate public health risks. Dont think any of the coauthors were involved as they seem to have affiliations in India.
Amino acid mutations PB1-V719M and PA-N444D combined with PB2-627K contribute to the pathogenicity of H7N9 in mice
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-024-01342-6
PB2-E627K mutation alone not sufficient to ???? virulence of H7N9 in mice. Combinations with PB1-V719M and/or PA-N444D mutations significantly enhanced H7N9 virulence + ???? polymerase activity. virulence in H7N9 is a polygenic trait.
Strong and consistent effects of waterbird composition on HPAI H5 occurrences across Europe
https://esajournals.onlinelibrary.wiley.com/doi/10.1002/eap.3010
Interesting to see a study from some years ago (old HPAI strains) reappear (with current panzootic data) with similar conclusions. The community composition of wild birds has an impact on HPAI in Europe.
2019 study: https://besjournals.onlinelibrary.wiley.com/doi/full/10.1111/1365-2656.12997
H5N1 avian influenza: tracking outbreaks with real-time epidemiological data
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00414-6/fulltext
Activation of Antiviral Host Responses against Avian Influenza Virus and Remodeling of Gut Microbiota by rLAB Vector Expressing rIL-17A in Chickens
https://pubs.acs.org/doi/10.1021/acsinfecdis.4c00377
Alternative approach to control avian influenza: cytokine-based approaches to augment antiviral host defense. Chicken cytokine IL-17A mediated selective expansion of beneficial gut microbiota > healthy microbiome > ???? AIV immune protection in chicken
Global changes in the epidemiology of Highly Pathogenic Avian Influenza Viruses
https://jpsad.com/index.php/jpsad/article/view/73
Short piece. Outlines concerns regarding reassortment & mutation of clade 2.3.4.4b H5N1 HPAIV’s. Suggests several mitigation & management measures to reduce human pandemic potential.
H9N2 Influenza A Viruses Found to be Enzootic in Punjab Pakistan’s Bird Markets with Evidence of Human H9N2 Nasal Colonization
https://www.sciencedirect.com/science/article/pii/S1201971224002170
H9N2 continues to circulate endemically in Asian poultry. 6.3% of poultry samples +ve, of which 73.9% H9N2 in Punjab Pakistan. Concerningly, 2 human cases identified. Scope for surveillance, prevention, control.
Transmission dynamics of avian influenza viruses in Egyptian poultry markets
https://www.nature.com/articles/s44298-024-00035-3
3,971 samples from poultry, wild birds, & environment across 4 Egyptian live bird markets. 17.4% +ve for AIV H5N1, H9N2, H5N8. Poultry w ????prevalence (42.2%) than wild birds (34.4%). Environmental samples +ve efor AIV. Fluctuating IAV +ve rates over time..
Mapping the risk of introduction of highly pathogenic avian influenza to Swedish poultry
https://www.sciencedirect.com/science/article/pii/S0167587724001466
Assessed spatiotemporal risk of introduction of HPAIV to Swedish poultry from wild birds using data from 2016-2021. High variation between national & local risk for HPAIV introduction to poultry.
R229I substitution from oseltamivir induction in HA1 region significantly increased the fitness of a H7N9 virus bearing NA 292K
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2373314
Oseltamivir-resistant H7N9 contain NA 292K, and HA R229I. HA mutation has substantial impact on ????replication ability, neuraminidase enzyme activity, binding ability to α2,3 + α2,6, pathogenicity to mice, transcriptome response =fine-tuning HA–NA balance
Molecular Markers and Mechanisms of Influenza A Virus Cross-Species Transmission and New Host Adaptation
https://www.mdpi.com/1999-4915/16/6/883
Summary of genetic changes & mechanisms influencing interspecific adaptation, cross-species transmission, & pandemic potential of IAV. Includes discussion of phenotypic traits associated with airborne transmission of IAV.
A(H2N2) and A(H3N2) influenza pandemics elicited durable cross-reactive and protective antibodies against avian N2 neuraminidases
https://www.nature.com/articles/s41467-024-49884-9
1.37-11.2% seropositivity against H9N2 in occupationally exposed adults in China – can human influenza (H2N2, H3N2) infection protect against avian H9N2? Highest rate of AIV N2 antibodies in individuals aged ≥65 years. 1968 pandemic N2 but not recent N2 protected against avian H9N2..
Avian flu: cases on the decline in Europe, surveillance recommended in view of upcoming season
https://www.efsa.europa.eu/en/news/avian-flu-cases-decline-europe-surveillance-recommended-view-upcoming-season
Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA
https://www.mdpi.com/1420-3049/29/12/2801
Dual Reverse Transcription Recombinase Polymerase Amplification for simultaneous detection for HA and M2 genes of H5N1.
Pinnipeds and avian influenza: a global timeline and review of research on the impact of highly pathogenic avian influenza on pinniped populations with particular reference to the endangered Caspian seal (Pusa caspica)
https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1325977/full
HPAI is posing a major conservation concern for pinnipeds in S. America. Here a review on impacts of HPAI on pinnipeds, focus on Caspian seal, currently under threat from HPAI H5N1 transmitted from infected seabirds/waterbrids which share haul-outs.
Safety, Tolerability, and Immunogenicity of aH5N1 Vaccine in Adults with and without Underlying Medical Conditions
https://www.mdpi.com/2076-393X/12/5/481
Phase-III trial for ajuvanted H5N1 human vaccine. Adjuvanted H5N1 vaccine (aH5N1) approved for prophylaxis against the H5N1. Evaluated safety & immunogenicity of aH5N1 in 4 groups of adults of different age groups. All groups had ^ antibody response to aH5N1 regardless of age or health status. Clinically acceptable safety & tolerability profile.
Influenza A virus antibodies in dogs, hunting dogs, and backyard pigs in Campeche, Mexico.
https://pubmed.ncbi.nlm.nih.gov/38196021/
Dogs, pigs, tested for swine, human, avian influenza viruses via serology. No avian influenza detected.
Using an adaptive modeling framework to identify avian influenza spillover risk at the wild-domestic interface
https://www.nature.com/articles/s41598-024-64912-w
High resolution spatial & temporal transmission risk models for US inderpined by: weekly species-level waterfowl abundance, LPAI prevalence, number of poultry farms, relative biosecurity risks.
Molecular epidemiology and genetic evolution of avian influenza H5N1 subtype in Nigeria, 2006 to 2021
https://link.springer.com/article/10.1007/s11262-024-02080-9
HPAI in Nigeria 2006 – 2021: Clade 2.2 in 2006, 2.3.2, 2.3.2.1f afterwards, 2.3.4.4b in 2021.Related to other viruses in W. Africa, and Egypt. Widespread distribution. Interplay bw LBMs, free range poultry, wetlands
Phylodynamics of avian influenza A(H5N1) viruses from outbreaks in Brazil
https://www.sciencedirect.com/science/article/pii/S0168170224001084
Interrogation of 2344b sequences from Brasil poultry, wild birds, and mammals. Similar to viruses from Chile, Uruguay, and Argentina. PB2 D701N and Q591K detected, no resistance to NAIs.
An avian-origin internal backbone effectively increases the H5 subtype avian influenza vaccine candidate yield in both chicken embryonated eggs and MDCK cells
https://www.sciencedirect.com/science/article/pii/S0032579124005674
Developed inactivated vaccine candidate by recombining clade 2.3.4.4d and clade 2.3.4.4b strain.= complete protection against wild-type strain challenge. High-yield, easy-to-cultivate candidate donor as an internal gene backbone for vaccine development.
Genetic diversity of H5N1 and H5N2 high pathogenicity avian influenza viruses isolated from poultry in Japan during the winter of 2022-2023
https://doi.org/10.1016/j.virusres.2024.199425
Multiple incursion events, genotypes, and HA sublineages of 2344b in Japan. HA subclade G2c caused the largest number of outbreaks – from Asia & Russia. Many genotypes from reassortment w wild bird viruses from N. America + Eurasia
What You Should Know About Avian Influenza A (H5N1)
https://asm.org/Articles/2024/June/What-You-Should-Know-About-Avian-Influenza-A-H5N1
From emergence to endemicity: highly pathogenic H5 avian influenza viruses in Taiwan
https://www.medrxiv.org/content/10.1101/2024.06.19.24309176v1
Clade 2.3.4.4c in Taiwan, initial wave 2015/16. Yunlin county key source for outbreaks: shift to chicken-dominant circulation from initial bidirectional spread between chicken and domestic waterfowl central to endemicity
Highly Pathogenic Avian Influenza Virus A(H5N1) Clade 2.3.4.4b Infection in Free-Ranging Polar Bear, Alaska, USA
https://wwwnc.cdc.gov/eid/article/30/8/24-0481_article
H5N1 in bears: species, including American +Asia black bears, grizzly bears, Kodiak brown bears, and recent Polar Bear in Alaska. Dead H5N1 short-tailed shearwaters (which breed in Australia) found in location of polar bear, murres previously. Ingestion of infected seabirds?
Co-infection of H9N2 subtype avian influenza virus and QX genotype live attenuated infectious bronchitis virus increase the pathogenicity in SPF chickens
https://www.sciencedirect.com/science/article/pii/S0378113524001858
Synergistic effect of H9N2 and infectious bronchitis virus in poultry. (I found similar effects between LPAI and gammacoronaviruses in wild birds via qPCR studies and metagenomics). Live attenuated IBV vaccines may ???? IBV-associated clinical lesions due to co-infections + immunosuppressive factors?
Genetic and molecular characterization of H9N2 avian influenza viruses in Yunnan Province, Southwestern China
https://www.sciencedirect.com/science/article/pii/S0032579124006199
Very high prevalence of H9N2 in poultry markets in Yunnan Province – 42% of tissues, 4% of swabs. No H9N2 in poultry farms. Lots of genetic diversity. A558V common, 1 with E627V in PB2.
Whole genome sequencing of low pathogenicity avian influenza virus (H6N2) detected from a Brazilian teal (Amazonnetta brasiliensis) in Brazil, 2023
https://journals.asm.org/doi/full/10.1128/mra.00158-24
Great to see more data from S. America. H6N2 from a Brazilian Teal, with distinct S. American genome, indicating extensive circulation among South American wild birds
Continued Evolution of H10N3 Influenza Virus with Adaptive Mutations Poses an Increased Threat to Mammals
https://www.sciencedirect.com/science/article/pii/S1995820X24000841
In China, recent human cases of H10N3 and H10N8. In 2022, H10N3 in diseased chickens in China, with genomes associated with human cases, and internal genes from H9N2. HA Q222R and G228S double mutations in receptor-binding domain.
Insights into avian influenza A(H5N1) events: epidemiological patterns and genetic analysis
https://www.tandfonline.com/doi/full/10.1080/23744235.2024.2369152
Another paper by the italian group who are capitalising on the hard work of others… Not sure there is much new here, just an overview and perspective.
Climate change is helping the H5N1 bird flu virus spread and evolve
https://theconversation.com/climate-change-is-helping-the-h5n1-bird-flu-virus-spread-and-evolve-230361
I’m not sure I agree that the panzootic is driven by climate change as much as poultry practices, vaccination, silent spread, and ample spillover opportunities.
H19 influenza A virus exhibits species-specific MHC class II receptor usage
https://www.cell.com/cell-host-microbe/abstract/S1931-3128(24)00190-2
The 19th HA subtype… found in scaups in 2010 and 2013 in the USA. Rather than sialic acid, H19 binds to the MHC class II. Absolutely wild.
FOUR-WEEK ORAL ADMINISTRATION OF BALOXAVIR MARBOXIL AS AN ANTI-INFLUENZA VIRUS DRUG SHOWS NO TOXICITY IN CHICKENS
https://doi.org/10.1638/2023-0103
Baloxivir is a newer generation antiviral for human influenza, targeting RdRp. Reduces mortality and virus secretion from HPAIV-infected chickens w/o long term toxicity. Treatment option for use in HPAIV-infected endangered birds?
Epidemiological dominance of the most virulent HPAIV H5N1 clade 2.3.4.4b strains: insights from experimental infections of Pekin ducks (Anas platyrynchos)
https://www.researchsquare.com/article/rs-4486200/v1
Contrary to the “avirulence hypothesis”, the most virulent genotypes in ducklings showed epidemiological dominance in the field. Rather, “virulence-transmission trade-off’ model for HPAI panzootic in Germany = pop size of susceptible hosts not limiting factor for spread. ???? host reservoirs due to fatal losses or gradually increasing population immunity in wild birds required.
Highly pathogenic avian influenza A(H5N1) virus infections on fur farms connected to mass mortalities of black-headed gulls, Finland, July to October 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.25.2400063
In 2023, 162 animals on 27 fox farms positive for HPAI. Many had ecrosuppurative bronchointerstitial pneumonia. Foxes and gulls in same area all had EA-2022-BB genotype and clustered together. Some mammalian adaptive mutations detected.
One health, many interpretations: vaccinating risk groups against H5 avian influenza in Finland
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2024.29.25.2400383#html_fulltext
In response to the large outbreaks of HPAI in foxes in Finland, the Finnish National PH institute is recommending HPAI vaccination for certain risk groups.
- Persons in contact with farmed fur animals;
- Persons in contact with poultry;
- Persons handling sick or dead animals or cleaning the related facilities;
- Persons in charge of ringing birds;
- Person taking care of birds in animal care facilities;
- Persons working with birds in bird or livestock farms;
- Veterinarians working in the public sector;
- Laboratory personnel working with testing of avian influenza;
- Close contacts of confirmed or suspected human avian influenza cases.
Global Antigenic Landscape and Vaccine Recommendation Strategy for Low Pathogenic Avian Influenza A(H9N2) Viruses
https://www.journalofinfection.com/article/S0163-4453(24)00133-6/fulltext
New approach for predicting H9 antigenic clusters. Antigenic relationship for H9N2, 1966-2022 =10 major antigenic clusters, 4 novel clusters were generated in China in the past decade
Bird flu: First person with confirmed H5N2 infection dies
https://www.bmj.com/content/385/bmj.q1260.full
Documents first confirmed human case of H5N2 IAV in a person who has since died in Mexico City.
Linked to: https://www.who.int/emergencies/disease-outbreak-news/item/2024-DON520
Zoonotic infections by avian influenza virus: changing global epidemiology, investigation, and control
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(24)00234-2/abstract
Changing epidemiology of human AIV infections. 2050 human cases since 2013 across diversity of subtypes, including H5Nx, H7N9, etc. With insights into human infections in China
Natural Infection with Highly Pathogenic Avian Influenza A/H5N1 Virus in Pet Ferrets
https://www.mdpi.com/1999-4915/16/6/931
5 ferrets in one household in Poland positive for HPAI. First natural infection in pet ferrets, concurrent w outbreaks of HPAI in Polish cats in 2023. Liver, lungs, kidneys were congested, change in brain tissue consistency.
To respond to the threat of avian influenza, look back at lessons learned from COVID-19
https://www.nature.com/articles/s41591-024-03106-3
Five communication + public health lessons from COVID-19 pandemic that could apply to tackling an AIV strain with pandemic potential. Lessons could compensate for a near-term shortage of vaccines and targeted therapeutics.
Zoonotic Animal Influenza Virus and Potential Mixing Vessel Hosts
https://www.mdpi.com/1999-4915/15/4/980
Comprehensive summary of potential mixing vessel or intermediate hosts for zoonotic influenza viruses. Found avian and swine influenza viruses are of high zoonotic potential, while influenza viruses of bovine, equine, canine, and bat origin are of low zoonotic risk.
H5N1: international failures and uncomfortable truths
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(24)01184-X/fulltext
Evaluates international response to US H5N1 outbreak and associated challenges. Suggests that One Health, though often acknowledged, is rarely prioritised and operationalised. Consider this a missed opportunity to respond to and prevent pandemic threats.
Building global preparedness for avian influenza
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(24)00934-6/abstract
Preparedness measures for AIV = adequate antiviral stockpiles, strengthening supply chains for PPE, enhancing detection of epidemiological, clinical, and laboratory signals via vets, health-care workers, and laboratory testing.
Phylogenetic and mutational analysis of H10N3 avian influenza A virus in China: Potential Threats to Human Health
https://www.frontiersin.org/articles/10.3389/fcimb.2024.1433661/abstract
*Full article yet to be published..
Large-Scale Serological Survey of Influenza A Virus in South Korean Wild Boar (Sus scrofa)
https://link.springer.com/article/10.1007/s10393-024-01685-8
7,209 wild boars in South Korea (2015-19) for IAV. 3.5% sero positive. 23 pdmH1N1, 6 human seasonal H3N2, 3 classical swine H1N1, 30 triple reassortants, 7 swine-origin H3N2 variant. Not just domestic pigs.
Hemagglutinin Glycosylation Pattern-Specific Effects: Implications for The Fitness of H9.4.2.5-branched H9N2 Avian Influenza Viruses
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2364736
Compared adaptive phenotypes H9N2 AIV mutants with different HA glycosylation patterns. Results indicate variations in glycosylation level impact antigenic drift which suggests that changes in number of glycans on HA can modulate receptor affinity and antigenicity of H9N2 AIVs, but also affect their stability and multiplication. Important findings for glycosylation-dependent vaccine design.
N-glycosylation on hemagglutinin head reveals inter-branch antigenic variability of avian influenza virus H5-subtypes
https://www.sciencedirect.com/science/article/pii/S0141813024037061
Assessment of primary glycosylation sites, incl 140 N, 156 N, 170 N in antigenic epitopes of H5N1. Inactivated recombinant strains = closer antigenicity compared to those w identical N-glycosylation patterns = inter-branch antigenic diversity of H5.
Genome sequences of H7N9 avian influenza virus in poultry-related environment in Henan Province in 2023
https://rs.yiigle.com/cmaid/1504205
In Chinese. Isolated three high avian pathogenicity H7N9 AIV strains from a live poultry market in Xuchang city, China in February 2023. No significant increase in mutations related to the binding ability to human receptors, mammalian pathogenicity, viral transmissibility, or drug resistance as compared with previous representative strains causing human or avian infection.
Isolation and identification of three strains of H5N6 avian influenza virus in Yunnan province in 2022 and analysis of the hemagglutinin and neuraminidase genes characteristics
https://rs.yiigle.com/cmaid/1500491
In Chinese. Novel high pathogenic H5N6 AIV subtype identified in three environment samples from a live poultry market in Yunnan province, China in 2022. Lacked receptor binding characteristics feasible to infect humans.
An investigation on avian influenza virus distribution in poultry-related environment in Nanping city
https://rs.yiigle.com/cmaid/1500534
In Chinese. Profiled distribution of AIV in poultry-related environments in Nanping city, China between December 2021 and December 2023. Positive rate of FluA in Nanping city was higher in autumn-winter season and in drinking water and faeces samples. Places where multiple types of poultry clustered, such as live poultry markets and slaughterhouses had a higher diversity of AIV’s.
Outbreak Reports: A Retrospective Investigation of a Case of Dual Infection by Avian-Origin Influenza A (H10N5) and Seasonal Influenza A (H3N2) Viruses — Anhui Province, China, December 2023–January 2024
https://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2024.106
Documents first human case of co-infection with H10N5 and seasonal H3N2 influenza viruses. Epidemiological investigations identified H10N5 in environmental samples linked to patient, but no transmission to close contacts occurred. Should correspond to: https://www.who.int/emergencies/disease-outbreak-news/item/2024-DON504
Fatal Infection in Ferrets after Ocular Inoculation with Highly Pathogenic Avian Influenza A(H5N1)
https://wwwnc.cdc.gov/eid/article/30/7/24-0520_article
Ocular inoculation of a clade 2.3.4.4b highly pathogenic H5N1 AIV caused severe and fatal infection in ferrets. Virus was transmitted to ferrets in direct contact. Highlights potential capacity of these viruses to cause human disease after either respiratory or ocular exposure.
The evolution of H5N1 influenza viruses in Indonesia to mammalian hosts: a review of molecular markers
https://www.tandfonline.com/doi/full/10.1080/00439339.2023.2294268
Found H5N1 viruses from various species in Indonesia have developed several important mammalian adaptation markers that enhance viral attachment to the α2,6 receptor, polymerase activity and pathogenicity in mammals, including humans.
A Chymotrypsin-Dependent Live-Attenuated Influenza Vaccine Provides Protective Immunity against Homologous and Heterologous Viruses
https://www.mdpi.com/2076-393X/12/5/512
Live-attenuated vaccine via mutating cleavage site of IAV. Effective protected mice against lethal doses of H1N1 and H5N1 post mucosal administration = highly effective broad-spectrum protective activity
Cross-protective efficacy and safety of an adenovirus-based universal influenza vaccine expressing nucleoprotein, hemagglutinin, and the ectodomain of matrix protein 2
https://www.sciencedirect.com/science/article/pii/S0264410X2400481X?via%3Dihub
Adenovirus-based universal influenza vaccine has efficacy and safety. Provide cross-protection in mice against various IAV subtypes, including H5N1, even at doses lower than those previously known to be effective
Avian influenza and gut microbiome in poultry and humans: A “One Health” perspective
https://www.sciencedirect.com/science/article/pii/S2667325823003643
Interaction between the poultry + human gut microbiome and AIV infection = surveillance program for the poultry or human gut microbiome might serve as a sentinel for monitoring the overall risk of AIV infection.
Effects of the Glycosylation of the HA Protein of H9N2 Subtype Avian Influenza Virus on the Pathogenicity in Mice and Antigenicity
https://www.researchgate.net/publication/380696332_Effects_of_the_Glycosylation_of_the_HA_Protein_of_H9N2_Subtype_Avian_Influenza_Virus_on_the_Pathogenicity_in_Mice_and_Antigenicity
Combinations of mutations and glycosylation modification site significantly affect the antigenicity, pathogenicity in mice, of H9N2. Sites which ???? pathogenicity ????expression proinflammatory factors in mice.
Mapping African Swine Fever and Highly Pathogenic Avian Influenza Outbreaks along the Demilitarized Zone in the Korean Peninsula
https://onlinelibrary.wiley.com/doi/full/10.1155/2024/8824971
HPAI risks in Korean DMZ shaped by precipitation and mean temperature from winter to spring and land use. cross-border collaboration + environmental and epidemiological insights to control animal disease in DMZ.
Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04040-9
Rapid tests, with quick response, simple operation, strong specificity, high sensitivity, good repeatability, and stability. Found suitable for early and rapid diagnosis of AIV.
Chicken UFL1 Restricts Avian Influenza Virus Replication by Disrupting the Viral Polymerase Complex and Facilitating Type I IFN Production
https://doi.org/10.4049/jimmunol.2300613
Chicken UFM1-specific ligase 1 restricts AIV replication by disrupting the viral polymerase complex and facilitating type I IFN production, which provides new insights into the regulation of AIV replication in chickens.
Avian influenza viruses in New Zealand wild birds, with an emphasis on subtypes H5 and H7: Their distinctive epidemiology and genomic properties
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0303756
Great to see this study from NZ colleagues on LPAI H5/H7. Of interest is that for neither H5 nor H7 do sequences from AUS/NZ fall into the same lineage. Likely 2 separate introductions, and little connectivity between.
Massive outbreak of Influenza A H5N1 in elephant seals at Península Valdés, Argentina: increased evidence for mammal-to-mammal transmission
https://www.biorxiv.org/content/10.1101/2024.05.31.596774v1
Illuminating results from marine mammal HPAI in S. Am. (1) Marine mammal clade = mammal<->mammal transmission (2) different evolutionary rate in mammal clade compared to birds (3) specific mutations for virulence, mammal adaptation? Concerning situation
In discussions with the authors, all samples were collected into inactivating media, so no chance of an isolate for ferret experiments. But the human case in Chile, which was part of the same clade, has been put in ferrets, and does transmit between ferrets via direct contact, but not respiratory/droplet transmission.
Highly pathogenic avian influenza A(H5N1) virus of clade 2.3.4.4b isolated from a human case in Chile causes fatal disease and transmits between co-housed ferrets
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2332667
Assessment of Survival Kinetics for Emergent Highly Pathogenic Clade 2.3.4.4 H5Nx Avian Influenza Viruses
https://www.mdpi.com/1999-4915/16/6/889
How long can HPAI last? Incubated virus at temps rep N. European winter (4C), summer (20C), S. European summer (30C). Lower temperature prolonged virus survival
IAVCP (Influenza A Virus Consensus and Phylogeny): Automatic Identification of the Genomic Sequence of the Influenza A Virus from High-Throughput Sequencing Data
https://www.mdpi.com/1999-4915/16/6/873
Bioinformatic pipeline implementing flexible analysis of the segmented IAV genome, obtaining a representative consensus from raw reads, and a straightforward method for detecting possible reassortment through phylogenetic tree construction. Most have their own pipelines and/or use IRMA, so not sure how this will compete with established approaches.
Specificity of the interaction between Neuraminidase N1 of the avian influenza A virus H1N1 1918 and a2-3 or a2-6 glycan receptors of avian and human cell targets
https://re.public.polimi.it/handle/11311/1266522
## No access
Outbreaks of H5N1 High Pathogenicity Avian Influenza in South Africa in 2023 Were Caused by Two Distinct Sub-Genotypes of Clade 2.3.4.4b Viruses
https://www.mdpi.com/1999-4915/16/6/896
In S. Africa: H5Nx, H7Nx, H9Nx, H11Nx, H6N2, and H12N2 in wild birds and ostriches in 2023, but H5Nx predominant. SA13 in coastal seabirds,SA15 caused chicken outbreaks. Great overview of the situation from the S. Africans
The H5 subtype of avian influenza virus jumped across species to humans – a view from China
https://www.journalofinfection.com/article/S0163-4453(24)00127-0/fulltext
Thoughts on the American dairy cow situation from chinese authors. Not that useful.
Highly pathogenic Avian Influenza H5N8 and H5N1 outbreaks in Algerian avian livestock production.
https://www.sciencedirect.com/science/article/pii/S0147957124000791
2 HPAI H5 outbreaks in Algerian poultry: 2020-2021 and 2022-2023 – 70% mortality due to H5N8, 40% due to H5N1. Systemic congestive-hemorrhagic syndrome in poultry.
The H4 subtype of avian influenza virus: a review of its historical evolution, global distribution, adaptive mutations and receptor binding properties
https://www.sciencedirect.com/science/article/pii/S0032579124004929
Review of the historical evolution, global distribution, adaptive mutations, receptor-binding preferences, and host range of H4 AIV.
Avian Influenza outbreaks: Human infection risks for beach users – One health concern and environmental surveillance implications
https://www.sciencedirect.com/science/article/pii/S0048969724038397
Highly Pathogenic Avian Influenza A(H5N1) in Animals: A Systematic Review and Meta-Analysis
https://www.sciencedirect.com/science/article/pii/S2052297524002233
RNF216 Inhibits the Replication of H5N1 Avian Influenza Virus and Regulates the RIG-I Signaling Pathway in Ducks
https://journals.aai.org/jimmunol/article-abstract/doi/10.4049/jimmunol.2300540/266925/RNF216-Inhibits-the-Replication-of-H5N1-Avian
#cant seem to access
Biological Characteristics of H6N1 Subtype Avian Influenza Virus from 2019 to 2022 in China
https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2024.09.015
English abstract. Think the text is in Chinese – cant get pdf to load. They found some H6 viruses.
COBRA HA and NA vaccination elicits long-live protective immune responses against pre-pandemic H2, H5, and H7 influenza virus subtypes
https://www.sciencedirect.com/science/article/pii/S0042682224001405
Mice vaccinated with COBRA H2, H5, H7, N1, N2, fully protected against lethal challenge with H5N6 influenza virus. Cross-reactive IgG antibodies against wild-type H2, H5, H7, N1 N2 proteins. Protective antibodies up to 4 months.
A geospatial perspective towards the role of migratory birds and poultry in the spread of Avian Influenza
https://www.researchsquare.com/article/rs-4451337/v1
Review integrating bird migration, poultry trade, and HPAI movement 2020-2023. Typically uninformed – no recent literature included. I mena, there have been a number of phylogenetic studies working this out very carefully – none cited. Just trying to jump on the bandwagon.
Pandemic preparedness through vaccine development for avian influenza viruses
https://www.tandfonline.com/doi/full/10.1080/21645515.2024.2347019
Review on pandemic preparedness for avian influenza viruses – vaccines in animal models and clinical trials on H5N1, H7N9, and H9N2 vaccines in humans
Proximal Origin of Epidemic Highly Pathogenic Avian Influenza H5N1 Clade 2.3.4.4b and Spread by Migratory Waterfowl
https://www.preprints.org/manuscript/202406.0060/v1
Absolutely total deranged bs.
“The proximal origins of HPAI H5N1 Clade 2.3.4.4b may be the USDA Southeast Poultry Research Laboratory (SEPRL) in Athens, Georgia and the Erasmus Medical Center in Rotterdam, the Netherlands.”
Reducing the risk of highly pathogenic avian influenza A virus H5N1 transmission during the Hajj
https://www.nature.com/articles/d41591-024-00042-0
The Hajj provides a unique opportunity to fully implement a ‘One Health’ approach to mass-gathering preparedness that can improve H5N1 surveillance and assist with other zoonosis. Risks and recommendations provided.
Diversity of Genotypes and Pathogenicity of H9N2 Avian Influenza Virus Derived from Wild Bird and Domestic Poultry
https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1402235/abstract
# no access yet.
11 H9N2 viruses from overwintering wild birds and their proximate domestic poultry in Yunnan Provence, China
First Detection of Highly Pathogenic Avian Influenza Virus in a Crested Caracara
https://meridian.allenpress.com/rapt/article-abstract/58/2/266/498089/First-Detection-of-Highly-Pathogenic-Avian?redirectedFrom=fulltext
# no access.
Re-evaluating efficacy of vaccines against highly pathogenic avian influenza virus in poultry: A systematic review and meta-analysis
https://www.sciencedirect.com/science/article/pii/S2352771424000405?via%3Dihub
Meta-analysis of experimental trials to assess efficacy of HPAI vaccines. Vaccines prevent mortality (78% to 97%) – vaccine platforms and match (or mismatch) at play. What about transmission?
Evaluation of the immune effect of a triple vaccine composed of fowl adenovirus serotype 4 fiber-2 recombinant subunit, inactivated avian influenza (H9N2) vaccine, and Newcastle disease vaccine against respective pathogenic virus challenge in chickens
https://www.sciencedirect.com/science/article/pii/S1056617124000096
Triple vaccine (Adeno, NDV, H9N2) could provide up to 100% immune protection against 3 viruses in chickens without interference. No viral shedding detected in larynx and cloaca on the fifth day after challenge.
A two-strain avian–human influenza model with environmental transmission: Stability analysis and optimal control strategies
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4151711
Developed mathematical model to determine optimal control strategies if AIV mutates to sustain human-human transmission and found quarantining infected humans is the most cost-effective strategy.
Parallel evolution in the emergence of highly pathogenic avian influenza A viruses
https://www.nature.com/articles/s41467-020-19364-x
Old paper, from before the updates. Parallel emergence of HP AIV may be facilitated by permissive or compensatory mutations occurring across the viral genome. mutational panel = reveal new links bw virulence evolution and other traits = prediction of future HP events?
Risk assessment of influenza transmission between workers and pigs on US indoor hog growing units
https://www.sciencedirect.com/science/article/pii/S0167587724001181
Not avian influenza, but perhaps useful regardless. Influenza transmission between workers and pigs on US indoor hog farms: Very low and Extremely low for H1N1, H1N2, H3N2. Control methods needed to ????risks of inter-species influenza transmission. Important given 2009 pandemic was pigs to humans.
Severe Avian Influenza A H5N1 Clade 2.3.4.4b Virus Infection in a Human with Continuation of SARS-CoV-2 Viral RNAs
https://www.hindawi.com/journals/tbed/2024/8819622/
Co-infections certainly happen – here HPAI H5N1 and COVID in a farmer in China with exposure to sick poultry. Patient recovered, and discharged after 44 days, after sportive and symptomatic treatment and use of antiviral drugs.
Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance
https://www.biorxiv.org/content/10.1101/2024.05.23.595634v1
Really exciting stuff. In the past, ferret experiments to try to reveal mutations important for human transmission. Lead to mortatorim on gain of function research. Here, they make all possible mutations in HA and then assess the phenotypes of all the mutations. So now we know what SNPs potentially mean. Data useful for tracking mutations in 2344b!
Development of a Fully Protective Pandemic Avian Influenza Subunit Vaccine in Insect Pupae
https://www.mdpi.com/1999-4915/16/6/829
Here, alternative technology for manufacturing subunit influenza HA-based vaccines by using insect pupae w baculovirus vectors. Vaccinated birds had no clinical disease, but shedding still present.
Pathological and phylogenetic characteristics of fowl AOAV-1 and H5 isolated from naturally infected Meleagris Gallopavo
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04029-4
Detection of avian paramyxovirus 1 and HPAI H5N1 2344b in turkeys in Egypt with respiratory signs and mortality. congestion and hemorrhage in the lungs, liver, and intestines with leukocytic infiltration. Endemic viruses in egyptian poultry is a real issue.
Characterizing the domestic-wild bird interface through camera traps in an area at risk for avian influenza introduction in Northern Italy
https://www.sciencedirect.com/science/article/pii/S0032579124004711
Published version of a preprint already reviewed. Important to understand the wild bird: poultry interface. Here, identification of wild bird species in poultry house surroundings and characterize the spatiotemporal patterns of visits: 27 different species – magpies, pheasants, doves most common.
Red knots in Europe – a dead end host species or a new niche for highly pathogenic avian influenza?
https://www.biorxiv.org/content/10.1101/2024.05.21.593879v1.abstract
Red Knot is a species we target in our spring enhanced surveillance, and from our work on LPAI has “higher” prevalence within the waders. Here, great overview of discrete genotype of HPAI in Knots in German Wadden Sea in 2020 – HPAI H5N3 reassortant in the knots, which was not detected elsewhere.
Pathogenicity and Transmission of Novel Highly Pathogenic H7N2 Variants Originating from H7N9 Avian Influenza Viruses in Chickens
https://www.sciencedirect.com/science/article/pii/S0042682224001429
Four HPAI H7N2 isolated in China during 2019 = reassortants with H7N9-derived HA genes and H9N2-derived NA genes. HA genes may be a critical virulence contributor to novel H7 avian influenza viruses.
Human neutralizing antibodies target a conserved lateral patch on H7N9 hemagglutinin head
https://www.nature.com/articles/s41467-024-48758-4
H7N9 caused thousands of human cases, with 30% CFR. Here, isolation of 4 HA-reactive mAbs: 3 directed to globular head + 1 to stalk. Description of mAbs and experiments to reveal effects. Overall, antibodies to a conserved lateral HA1 supersite combined with a HA2-directed non-neutralizing mAb augment protection.
Global Dynamics Analysis of Non-Local Delayed Reaction-Diffusion Avian Influenza Model with Vaccination and Multiple Transmission Routes in the Spatial Heterogeneous Environment
https://link.springer.com/article/10.1007/s12346-024-01057-1
Modelling study shows prolonging the incubation period, controlling the movement of infected poultry, and regular disinfecting the environment are all effective ways to prevent avian influenza outbreaks. Its maths heavy and it wasn’t immediately obvious what the input data was.
Highly pathogenic avian influenza A (H5N1) virus outbreak in Peru in 2022–2023
https://www.sciencedirect.com/science/article/pii/S2772431X24000224
More HPAI H5N1 genomes from Peru. In sequence from sea lion: I352K and 1368C>T mutations in the HA gene and in the PB2 D701N and Q591K
Panzootic HPAIV H5 and risks to novel mammalian hosts
https://www.nature.com/articles/s44298-024-00039-z
In order to mitigate the zoonotic risk of HPAI H5, essential to understand + monitor at avian-mammal interface.
Detection of a reassortant swine- and human-origin H3N2 influenza A virus in farmed mink in British Columbia, Canada
https://www.biorxiv.org/content/10.1101/2024.05.27.596080v1
Not avian influenza, but I have included it as I think its an important study to be aware of. Reassortant H3N2 from reassortment of swine H3N2 (clade 1990.4h), human seasonal H1N1 (pdm09), and swine H1N2 (clade 1A.1.1.3) found in a mink farm in Canada. been subsequently observed in swine and poultry in N. Am. closer surveillance in mink needed!
Deciphering bat influenza H18N11 infection dynamics in male Jamaican fruit bats on a single-cell level
https://www.nature.com/articles/s41467-024-48934-6
Not avian influenza, but a really exciting study of bat H18N11 in Jamaican Fruit Bats. We know that bats seem to tolerate infections with viruses really well. H18N11 infection = moderate induction of interferon-stimulated genes and transcriptional activation of immune cells. human leukocytes, particularly macrophages, were also susceptible to H18N11.
Effect of Enteromorpha polysaccharides on gut-lung axis in mice infected with H5N1 influenza virus
https://www.sciencedirect.com/science/article/pii/S0042682224000527?via%3Dihub
Demonstrated enteromorpha polysaccharides (sugar) potential in protecting host from HPAI H5N1. Found body weight of mice recovered and pathological damage to the lung and intestine was reduced after EPP inclusion.
Infection dynamics of subtype H9N2 low pathogenic avian influenza A virus in turkeys
https://www.sciencedirect.com/science/article/pii/S0042682224001454
Turkeys infected w H9N2 in a target-cell limited model. Turkeys had a different set of infection characteristics, compared with humans and ponies. Clearance rate similar bw turkeys and ponies, cell death and tramission similar bw turkeys and humans. Paper is weird. Where does ponies even come from?
Assessing avian influenza surveillance intensity in wild birds using a One Health lens
https://www.sciencedirect.com/science/article/pii/S2352771424000867
AIV surveillance in Ontario, Canada. 2562 samples. Identify spatial variations in surveillance intensity relative to human population density, poultry facility density, and wild mallard abundance. Data to improve Onehealth response.
Prevalence and risk factor for H9N2 avian influenza virus in poultry retail shops of Madhya Pradesh
https://link.springer.com/article/10.1007/s13337-024-00865-y
H9N2 endemic in poultry in Asia. 500 poultry tissue and 700 environmental samples in India. 9% prevalence (by egg isolation), 50% districts positive. Risk factors for H9N2 identified – e.g. procuring birds from wholesaler.
Proactive surveillance for avian influenza H5N1 and other priority pathogens at mass gathering events
https://www.thelancet.com/journals/lanpub/article/PIIS2468-2667(24)00103-8/fulltext
Public health preparedness and careful planning and surveillance before and during mass gathering events remain important for preventing major outbreaks. Call for proactive surveillance for H5N1 in humans.
To vaccinate or not against highly pathogenic avian influenza?
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(24)00107-1/fulltext
To vaccinate or not vaccinate against HPAI H5N1. Lots of factors, and of course contexts to consider. Can be a useful tool to control HPAI if done properly, but important to avoid further evolution and silent spread.
Avian ‘Bird’ Flu – undue media panic or genuine concern for pandemic potential requiring global preparedness action?
https://www.sciencedirect.com/science/article/pii/S1201971224001334
“Striking the right balance between the existing pandemic in birds with the pandemic potential for humans is the essence.” OneHeath framework critical.
A highly sensitive and accurate dual-channel fluorescent immunochromatographic assay for simultaneous quantitative detection of influenza A virus and adenovirus antigens
https://www.sciencedirect.com/science/article/pii/S0026265X23011864
Rapid diagnostics is the future for animal disease emergencies. Here, a dual dual-channel immunochromatographic assay for both influenza and adenovirus in poultry from pharyngeal swab samples.
Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 Avian Influenza Virus (AIV)
https://www.jbc.org/article/S0021-9258(24)01896-9/fulltext
H9N2 is the main epidemic subtype in Chinese poultry despite vaccination programs. Here, identify CD8+ T cell epitopes targeting H9N2 to lay the foundation for the potential development of T-cell epitope vaccines.
Guanylate-binding protein 1 inhibits inflammatory factors produced by H5N1 virus through Its GTPase activity
https://www.sciencedirect.com/science/article/pii/S0032579124003791
Guinea pig guanosine monophosphate binding protein 1 (gGBP1) downregulates cytokine production induced by influenza. Here shown to be important for H5N1 in guinea pig cell lines.
Sequence-based epitope mapping of high pathogenicity avian influenza H5 clade 2.3.4.4b in Latin America
https://www.frontiersin.org/articles/10.3389/fvets.2024.1347509/full
Lots of suggestion that HPAI in S.America is different (due to widespread marine mammal outbreaks). 3 major subtypes and eight sub-genotypes identified, with 3 potential antigenic variants, indicating the HA-C group as the dominant variant.
The complete coding sequence of Influenza A/Unknown/Chelyabinsk/206/H7N4
https://journals.asm.org/doi/10.1128/mra.00312-24
Characterisation of an H7N4 virus found in Russia. Potentially first H7 genome from Russia.
Nucleic acid detection and genomic sequence analysis of one H5N1 avian influenza virus from wide birds around Qinghai Lake.
http://www.flu.org.cn/en/article_detail?action=ql&uid=&pd=&articleId=21046
Link seems problematic?
US Public Health Preparedness and Response to Highly Pathogenic Avian Influenza A(H5N1) Viruses
https://jamanetwork.com/journals/jama/article-abstract/2819159
Survey of US state epidemiologists in 55 jurisdictions for H5N1 virus ID, monitoring, antiviral and vccines. In 50, human exposure to animals from backyard flocks (88%), wild birds (54%), and sick/dead mammals (18%)
Spatial and Temporal Characteristic Analysis and Risk Assessment of Global Highly Pathogenic Avian Influenza H5N8 Subtype
https://www.hindawi.com/journals/tbed/2024/5571668/
Found midlatitude areas (30-60 degrees) at higher risk of HPAI H5N8 occurrence. Key variables influencing occurrence are chicken density, duck density, population density, annual mean temperature and land cover. Note wild bird migration data not used in modelling.
Simultaneous construction strategy using two types of fluorescent markers for HVT vector vaccine against infectious bursal disease and H9N2 avian influenza virus by NHEJ-CRISPR/Cas9
https://www.frontiersin.org/articles/10.3389/fvets.2024.1385958/full
Demonstrated recombinant virus rHVT-VP2-HA provided 100% simultaneous protection against G2d lineage of Infectious Bursal Disease Virus and Y280 lineage of HPAI H9N2 (stops replication) in chickens.
Detection of clade 2.3.4.4b highly pathogenic H5N1 influenza virus in New York City
https://pubmed.ncbi.nlm.nih.gov/38747601/
Publication of a previously reviewed preprint. Multiple HPAI H5N1 genotypes detected in four different avian species in New York City highlighting risk of zoonotic infections extends into urban centres.
Development of a nucleoside-modified mRNA vaccine against clade 2.3.4.4b H5 highly pathogenic avian influenza virus
https://www.nature.com/articles/s41467-024-48555-z
mRNA lipid nanoparticle vaccine encoding HA of 2.3.4.4b H5N1 = strong T cell + antibody responses in mice, w neutralizing antibodies + broadly-reactive anti-HA stalk antibodies. Prevents morbidity and mortality of ferrets in challenge.
Avian influenza virus neuraminidase stalk length and haemagglutinin glycosylation patterns reveal molecularly directed reassortment promoting the emergence of highly pathogenic clade 2.3.4.4b A (H5N1) viruses
https://www.biorxiv.org/content/10.1101/2024.05.22.595329v1
What makes the 2.3.4.4b a panzootic virus? Here, revealed that the seven glycosylation sites in HA was critical in driving its pairing with long stalk N1 = increased fitness and pathogenicity. HA-NA pairing not stoichastic.
Risk assessment of a highly pathogenic H5N1 influenza virus from mink
https://www.nature.com/articles/s41467-024-48475-y
Important study investigating mammalian infection + transmission of HPAI isolated from the 2022 mink outbreak in spain. Direction transmission 75% contacts, airborne transmission 37.5% contacts. PB2 T271A important for mortality + airborne transmission. But sequence analysis found no known mutations associated with mammalian transmission
Pop Sci Summary: https://afludiary.blogspot.com/2024/05/nature-dispatch-risk-assessment-on-hpai.html
On-Site and Visual Detection of the H5 Subtype Avian Influenza Virus Based on RT-RPA and CRISPR/Cas12a
https://www.mdpi.com/1999-4915/16/5/753
New RT-RPA/CRISPR-based detection method allows for rapid detection of HPAI, and easy to use test strips. 80.70% positive detection rate across 81 clinical samples tested.
Efficacy of live and inactivated recombinant Newcastle disease virus vaccines expressing clade 2.3.4.4b H5 hemagglutinin against H5N1 highly pathogenic avian influenza in SPF chickens, Broilers, and domestic ducks
https://www.sciencedirect.com/science/article/pii/S0264410X24005279
NDV-vectored vaccine w 2.3.4.4b H5 HA developed and assessed. Vaxx achieved complete survival against HPAI and NDV challenges and significantly reduced viral shedding in chickens, decreased shedding in ducks. Doesn’t stop infection/transmission. Aligns with the strategy of Differentiating Infected from Vaccinated Animals (DIVA)
Building global preparedness for avian influenza
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(24)00934-6/abstract
Commentary on how to prepare for human infection with HPAI, suggestions for improved surveillance and detection.
H6N2 reassortant avian influenza virus isolate in wild birds in Jiangxi Province, China
https://link.springer.com/article/10.1007/s11262-024-02068-5
Uncommon for Asian LPAI viruses to have gene segments from Americas in my experience. Here the N2 of an H6N2 virus in China closely related to California bufflehead virus. More wild bird sampling +sequencing in Asia could resolve this.
Dual N-linked glycosylation at residues 133 and 158 in the hemagglutinin are essential for the efficacy of H7N9 avian influenza virus like particle vaccine in chickens and mice
https://www.sciencedirect.com/science/article/pii/S0378113524001305
N-linked glycosylation-engineered H7N9 virus like particle vaccines conferred complete protection against H7N9 viruses and and significantly suppressed virus replication and lung pathology in chickens and mice
Duration of Highly Pathogenic Avian Influenza Virus and Newcastle Disease Virus Infectivity in Dried Ornithologic Study Skins
https://meridian.allenpress.com/jwd/article/doi/10.7589/JWD-D-24-00010/500638
Can HPAI survive on ornithological study skins? At 4 weeks viable virus could not be detected on the prepared study skins (of poultry, which were infected)
Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses: Call for a change in H9N2 control policy
https://www.sciencedirect.com/science/article/pii/S2095311924001928
H9N2 is endemic in poultry in Asia, and causes human cases each year. Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses – time for an update? Vaccine pressure on virus evolution?
Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Virus in Seals, St. Lawrence Estuary, Quebec, Canada
https://wwwnc.cdc.gov/eid/article/30/6/23-1033_article
Previously summarised preprint, now published. In 2022, 209 dead/sick seals reported in Quebec (Harbor, Gray, Harp, Hooded seals). Many sick birds, so expected bird-> seal transmission. meningoencephalitis (100%), fibrinosuppurative alveolitis, multiorgan acute necrotizing inflammation.
A systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild avifauna of North America
https://www.biorxiv.org/content/10.1101/2024.05.06.592734v1
First comprehensive database following compliation of available literature reporting pathobiology of AIV’s in all wild birds in over a decade. = tool for researchers, providing generalized estimates of pathobiology for wild avian families, knowledge gap?
Epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift
https://academic.oup.com/femsre/advance-article/doi/10.1093/femsre/fuae014/7670612
Epic review of the epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift
Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0300862
Great to see more data on LPAI in shorebirds from South America. Looong branch lengths in the trees demonstrate clear lack of sampling/sequencing. Similar to both N. and S. American segments – long distance movement?
DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge
https://www.mdpi.com/2076-393X/12/5/538
pVAX-H5 DNA vaccine encoding a modified trimer of AIV H5N8 hemagglutinin. In BALB/c mice. high level of neutralizing antibodies and T-cell response. Both liquid and lyophilized forms provided 100% protection of immunized mice.
Infectivity of Wild-Bird Origin Influenza A Viruses in Minnesota Wetlands across Seasons
https://www.mdpi.com/2076-0817/13/5/406
How long to avian influenza viruses survive in the environment? limited evidence for the extended persistence of IAVs held in mesocosms in the field (in contrast to temperature controlled water in the lab). Context important for environmental studies
Structural and functional characterization of avian influenza H9N2 virus neuraminidase with a combination of five novel mutations
https://www.sciencedirect.com/science/article/pii/S0003986124001607
Combo of 5 novel amino acid substitutions in the NA, near the sialic acid binding site = ????substrate binding and altered surface characteristics to LPAI H9N2.
Nucleotide sequence as key determinant driving insertions at influenza A virus hemagglutinin cleavage sites
https://www.nature.com/articles/s44298-024-00029-1
Another banger from the Erasmus crew and Im fairly sure this is the final published version of a preprint I shared in the past. How are aa’s acquired when LPAI->HPAI. Nucleotide sequence is a key determinant of insertions in the HA CS. Indels readily detected in a consensus H5 LPAI CS at low frequency (but not for other LPAI). Great mechanism paper.
Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation
https://www.nature.com/articles/s41467-024-48470-3
Antibodies to Influenza A(H5N1) Virus in Hunting Dogs Retrieving Wild Fowl, Washington, USA
https://wwwnc.cdc.gov/eid/article/30/6/23-1459_article
Antibodies to H5N1 in 4/194 (2%) dogs from Washington, USA, that hunted wild birds. Historical data provided by dog owners showed seropositive dogs had high levels of exposure to waterfowl.
Iceland: an underestimated hub for the spread of high-pathogenicity avian influenza viruses in the North Atlantic
https://doi.org/10.1099/jgv.0.001985
Given 2 jumps of HPAI over Atlantic Ocean, role of Iceland important to be clarified. Diversity of HPAI in 2022 & 2023, incld novel introduction in 2023. Bridge for intercontinental introduction.
Pigs are highly susceptible to but do not transmit mink-derived highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b
https://www.biorxiv.org/content/10.1101/2023.12.13.571575v2
Nice follow-up to better understand transmission potential of HPAI H5N1 detected in mink farm, speculated to have been transmitting mammal-to-mammal. PIgs highly susceptible. Mammalian adaptive mutations PB2-E627K + HA-Q222L emerged at low frequencies. No transmission between pigs.
A multi-species, multi-pathogen avian viral disease outbreak event: Investigating potential for virus transmission at the wild bird – poultry interface
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2348521
Nice case study demonstrating how HPAI can go from infected poultry property into wild birds (here predators and scavenging birds). Also compound an HPAI outbreak with detection of paramyxovirus wild birds.
Update of the target list of wild bird species for passive surveillance of H5 HPAI viruses in the EU
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/sp.efsa.2024.EN-8807
Report from Sovon outlining target list of bird species for passive surveillance in EU. It would be good for us to develop something like this actually – ensure that jurisdictions are focussing on waterbirds/waterfowl/seabirds/shorebirds and raptors.
Novel Genotype of HA Clade 2.3.4.4b H5N8 Subtype High Pathogenicity Avian Influenza Virus Emerged at a Wintering Site of Migratory Birds in Japan, 2021/22 Winter
https://www.mdpi.com/2076-0817/13/5/380
Detection of HPAI H5N8 in Japan 2021/22 winter. 6/8 genes closely related to 2.3.4.4b H5N8 in G2a group = was responsible for outbreaks in poultry farms in November 2021 in Japan. 2 segments from LPAI. Infectivity of this virus different from G2a H5N8.
Spatio-temporal dynamics and drivers of highly pathogenic avian influenza H5N1 in Chile
https://www.frontiersin.org/articles/10.3389/fvets.2024.1387040/full
Using data from only 1 database in Chile, 7 significant clusters. H5N1 correlated w bird richness, human pop, distance to SAG’s office, and mean diurnal range. Negative correlation to a few metrics (isothermality, temperature annual range, precipitation of the warmest quarter, and coldest quarter). Take with grain of salt due to use of only 1 database, and not sure this is super appropriate in an active outbreak setting.
Avian Influenza Virus Infections in Felines: A Systematic Review of Two Decades of Literature
https://www.medrxiv.org/content/10.1101/2024.04.30.24306585v1
Rather timely review of HPAI in cats. cat-to-cat transmission has been demonstrated experimentally, and real-world outbreaks have been reported. Detections in cats have occurred for as long as HPAI H5 has been circulating.
Novel Avian Influenza A(H5N6) in Wild Birds, South Korea, 2023
https://wwwnc.cdc.gov/eid/article/30/6/24-0192_article
Novel 2.3.4.4b H5N6 viruses emeged in Korea. all 8 genes shared highest nucleotide identity (99.77%–100%) w 2.3.4.4b H5N6 in peregrine falcon in Japan. N6 similar to H5N6 found in humans and poultry in China.
Concurrent Infection with Clade 2.3.4.4b Highly Pathogenic Avian Influenza H5N6 and H5N1 Viruses, South Korea, 2023
https://wwwnc.cdc.gov/eid/article/30/6/24-0194_article
2.3.4.4b H5N6 and H5N1 simultaneously introduced into S. Korea at end of 2023, w broiler farm co-infected. Emerged H5N6 viruses spread coincidently throughout winter in East Asia
Mapping Genetic Markers Associated with Antigenicity and Host Range in H9N2 Influenza A Viruses Infecting Poultry in Pakistan
https://doi.org/10.1637/aviandiseases-D-23-00029
Can’t access full article.
Blowflies are potential vector for avian influenza virus at enzootic area in Japan
https://www.nature.com/articles/s41598-024-61026-1
Blowflies are attracted to decaying animals and feces, and migrate to lowland areas of Japan from northern regions in early winter, coinciding with HPAI season. 2.2% prevalence, w highest occurrence near a crane colony (14.9%). Indicator, or transmitter?
Viral Pathogen Detection in U.S. Game-Farm Mallard (Anas platyrhynchos) Flags Spillover Risk to Wild Birds
https://www.frontiersin.org/articles/10.3389/fvets.2024.1396552/abstract
Accepted, but no pdf available yet.
Seabird and sea duck mortalities were lower during the second breeding season in eastern Canada following the introduction of Highly Pathogenic Avian Influenza A H5Nx viruses
https://www.biorxiv.org/content/10.1101/2024.05.03.591923v1.abstract
In Canada, mortalities due to HPAI in 2023 were 93% lower vs 2022 (v positive news!) but encompassed a more taxonomically diverse array of species. Winter = waterfowl, summer = seabirds. 3 notable mortality events in 2023 (eg 1,646 Greater Snow Geese)
Joint FAO/WHO/WOAH preliminary assessment of recent influenza A(H5N1) viruses
https://www.who.int/publications/m/item/joint-fao-who-woah-preliminary-assessment-of-recent-influenza-a(h5n1)-viruses
“Individuals with activities that involve exposure to infected animals and/or contaminated environments are at higher risk and should take necessary precautions to prevent infection.18 At the present time, based on available information, WHO assesses the overall public health risk posed by A(H5N1) to be low, and for those with exposure to infected birds or animals or contaminated environments, the risk of infection is considered low-to-moderate”
Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus Infection in Domestic Dairy Cattle and Cats, United States, 2024
https://wwwnc.cdc.gov/eid/article/30/7/24-0508_article
Infected cattle experienced nonspecific illness, reduced feed intake and rumination, and an abrupt drop in milk production, but fatal systemic influenza infection developed in domestic cats fed raw (unpasteurized) colostrum and milk from affected cows.
Emergence and interstate spread of highly pathogenic avian influenza A(H5N1) in dairy cattle
https://www.biorxiv.org/content/10.1101/2024.05.01.591751v1
Great to see the analysis by the USDA and folks involved in the cattle outbreaks. Genomic analysis and epidemiological investigation showed a reassortment event in wild bird populations preceded a single wild bird-to-cattle transmission episode..
What is the pandemic potential of avian influenza A(H5N1)?
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099%2824%2900238-X/fulltext
“Although sequencing of viruses from the patient and cattle in Texas did not ring alarm bells regarding the potential of HPAI A(H5N1) clade 2.3.4.4b for sustained human transmission, the next flu pandemic—whether caused by an avian influenza A(H5N1) virus or otherwise—seems inevitable. The threat of a pandemic remains high, and we urge international leaders to reach an agreement on a pandemic accord before it is too late”
Evolutionary dynamics and comparative pathogenicity of clade 2.3.4.4b H5 subtype avian influenza viruses, China, 2021–2022
https://www.sciencedirect.com/science/article/pii/S1995820X24000609
Mostly 2.3.4.4b in China, include N1, N6, N8, but all likely evolved from H5N8. H5N1 have higher evolutionary rate 2021-22, more positive selected sites 2015-22. H5N1 antigenically distinct from H5N8/N6. Heterogeneous virulence in mammals.
Pathogen Surveillance in Swallows (family Hirundinidae): Investigation into Role as Avian Influenza Vector in Eastern Canada Agricultural Landscapes
https://meridian.allenpress.com/jwd/article/doi/10.7589/JWD-D-23-00167/500389
Swallows, abundant in agricultural ecosystems, have been proposed as possible (bridge) species for HPAI transmission between wild and domestic birds. All tested swallows negative for both HPAI and LPAI.
Phylogeography and gene pool analysis of highly pathogenic avian influenza H5N1 viruses reported in India from 2006 to 2021
https://link.springer.com/article/10.1007/s00705-024-06032-4
Co-circulation of many clades of HPAI H5N1 in India. 5 separate introductions of HPAI: via Indonesia or Korea (2002), Bangladesh (2009), Bhutan (2010), and China (2013, 2018), with 8 reassortant genotypes.
Cross-species spill-over potential of the H9N2 bat influenza A virus
https://www.nature.com/articles/s41467-024-47635-4
Some issue on the page, so no summary
Surveillance for highly pathogenic avian influenza A (H5N1) in a raptor rehabilitation center—2022
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0299330
At a raptor centre in 2022, 996 birds acros 20 different species were tested for HPAI, and 213 birds were confirmed HPAI positive. contributed 75% of the HPAI positive raptor detections within the state of Minnesota.
Detection of hemagglutinin H5 influenza A virus sequence in municipal wastewater solids at wastewater treatment plants with increases in influenza A in spring, 2024
https://www.medrxiv.org/content/10.1101/2024.04.26.24306409v1
Wastewater monitoring may be useful for HPAI monitoring. In USA, substantial increase in H5 detections. At 2 wastewater plants industrial discharges containing animal waste, including milk byproducts, were permitted to discharge into sewers.
Factors affecting highly pathogenic avian influenza vaccination practices at poultry farms in Tra Vinh, Vietnam
https://www.ejmanager.com/mnstemps/100/100-1698640057.pdf?t=1714391009
166 poultry farms with 14894 poultry in VietNam to determine socioeconomic and production characteristics to understand effect on HPAI vaccination practices. <50% of farms vaccinate.
Highly pathogenic avian influenza H5N1 virus infections in pinnipeds and seabirds in Uruguay: implications for bird-mammal transmission in South America
https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veae031/7645834
Good to see this published. HPAI viruses from Uruaguay suggest pinnipeds ancestral to birds. Viruses in South America may have spread from mammals to mammals and seabirds, revealing a new transmission route. Big implications!
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses
https://www.sciencedirect.com/science/article/abs/pii/S0166093424000661
Multiplex H5/7/9 rRT-PCR assay optimized to simultaneously detect H5, H7, and H9.
Epidemiological characteristics of human infections with avian influenza A(H5N6) virus, China and Laos: A multiple case descriptive analysis, February 2014–June 2023
https://www.canada.ca/content/dam/phac-aspc/documents/services/reports-publications/canada-communicable-disease-report-ccdr/monthly-issue/2024-50/issue-1-2-january-february-2024/ccdrv50i12a09-eng.pdf
From 2014-2023, 85 human cases with HPAI H5N6. Case fatality rate: 39%. increased frequency from 2021 to present day. 84% reported contact with birds prior to illness onset.
Modeling the transmission dynamics of H9N2 avian influenza viruses in a live bird market
https://www.nature.com/articles/s41467-024-47703-9
Great to see this modelling study of H9N2 in LBM. Many supplied birds arrive already exposed to H9N2, indigenous backyard chickens entering with pre-existing immunity. Susceptible chickens infected within one day at the market.
Repeatability and reproducibility of hunter-harvest sampling for avian influenza virus surveillance in Great Britain
https://www.sciencedirect.com/science/article/pii/S0034528824001450
Application of rapid diagnostics for samples collected from hunter-harvested waterfowl offers potential as an early warning system for HPAI.
Mapping the interaction sites of human and avian influenza A viruses and complement factor H
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1352022/full
human FH can bind directly to IAVs of both human and avian origin, and the interaction is mediated via the HA. Interaction overlapped with the receptor binding site. Important for understanding mechanisms of infection and protection.
Sequence-based epitope mapping of High Pathogenicity Avian Influenza H5 clade 2.3.4.4b in Latin America Unraveling Molecular and Antigenic Profile of Highly Pathogenic Avian Influenza H5 2.3.4.4b in Latin America
https://www.frontiersin.org/articles/10.3389/fvets.2024.1347509/abstract
No access yet.
Highly Pathogenic Avian Influenza A(H5N1) Virus : Identification of Human Infection and Recommendations for Investigations and Response
https://stacks.cdc.gov/view/cdc/153423
First human case of avian influenza A (H10N3) in Southwest China
https://www.researchsquare.com/article/rs-4181286/v1
Case report of first human case of H10N3. H10 seems to infect a broad host range. Patient w severe pneumonia, type I respiratory failure, complications w fungi & bacteria. four mutations potentially hazardous to human health.
Human infection caused by avian influenza A (H10N5) virus
https://www.sciencedirect.com/science/article/pii/S1684118224000744
Case report of human infection with H10N5. Patient co-infected with human H3N2. female farmer aged over 60, with underlying comorbidities, who experienced symptoms of cough, sore throat. The patient had a history of exposure to live poultry
Genetic drift and purifying selection shape within-host influenza A virus populations during natural swine infections
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1012131
Important not to forget pigs are important hosts for influenza (implicated in H1N1 pandemic). Similarities in patterns of genetic diversity and evolution b/w humans and pigs, including the role of stochastic processes in shaping within-host IAV dynamics.
Timor Leste has reported HPAI. It seems the diagnostics was done by ACDP 01/09/2022. 4 domestic birds. No lineage information.
https://wahis.woah.org/#/in-review/5644
Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation
https://www.researchsquare.com/article/rs-3716220/v1
Replication of avian influenza in mammalian cells is hindered by variation in ANP32. Cryo-electron microscopy of avian H5N1 FluPolA with human ANP32B =enhance understanding of molecular processes underpinning mammalian adaptations to avian influenza
Reply to León et al. (2024): Interpretation and Use of In-Field Diagnostics for High Pathogenicity Avian Influenza (HPAI) in Antarctica– A Cautionary Tale
https://www.preprints.org/manuscript/202404.1498/v1
We wrote a response to a recent preprint which claimed HPAI in apparently healthy penguins in Antarctica. Turns out they used an H5 assay which detects both LPAI and HPAI, so unclear what they found exactly.
Molecular characterization of the whole genome of H9N2 avian influenza virus isolated from Egyptian poultry farms
https://link.springer.com/article/10.1007/s00705-024-06018-2
H9N2 endemic in poultry, cause sporradic human cases. Common in Egypt. Virus from 2021: G1 sublineage of the Eurasian lineage, internals similar to Egyptian viruses. Subs in HA (191H and 234L) = predilection for attaching to human-like receptors
Evolution and Antigenic Differentiation of Avian Influenza A(H7N9) Virus, China
https://pubmed.ncbi.nlm.nih.gov/38640498/
Vaccination of birds against HPAI can help us tremendously, but if done improperly, may drive virus evolution. Here a detailed look at H7N9, and the conclusion that vaccination may be preventing reassortment. Understanding vaccine effects critical.
Highly pathogenic avian influenza A(H5N1) virus in a common bottlenose dolphin (Tursiops truncatus) in Florida
https://www.nature.com/articles/s42003-024-06173-x
In 2022, a stranded bottle-nosed dolphin in Florida tested positive for HPAI. Largest amount of virus in the brain (qPCR), neuronal necrosis and inflammation of the brain and meninges. S246N NA sub = ???? inhibition by oseltamivir.
Isolation and genetic characterization of multiple genotypes of both H5 and H7 avian influenza viruses from environmental water in the Izumi plain, Kagoshima prefecture, Japan during the 2021/22 winter season
https://www.sciencedirect.com/science/article/abs/pii/S0147957124000596
While samples from birds are critical for HPAI, environmental samples can provide additional data. Both H5Nx and H7 detected in environment water in Japan during the 2021/22 winter season when HPAI prev high.
Molecular Characterization of Non-H5 and Non-H7 Avian Influenza Viruses from Non-Mallard Migratory Waterbirds of the North American Flyways, 2006–2011
https://www.mdpi.com/2076-0817/13/4/333
Most avian influenza surveillnce systems focussed on Mallard. What is going on in other species? 1158 samples, 2006 & 2011 in USA, 87 LPAI. blue-winged teal, American wigeon, and American black duck species key players.
Contrasting dynamics of two incursions of low pathogenicity avian influenza virus into Australia
https://www.biorxiv.org/content/10.1101/2024.04.23.590662v1
Our study looking at recent virus incursions into Australia – H4 viruses found in shorebirds, seemed to stay in shorebirds and H10 viruses which appeared across australia pretty rapidly, and infected a large diversity of different hosts. Hopefully shed a bit more light on the process of virus entry, relevant for HPAI planning…
Bat-borne H9N2 influenza virus evades MxA restriction and exhibits efficient replication and transmission in ferrets
https://www.nature.com/articles/s41467-024-47455-6
A highly divergent bat H9 influenza (similar to avian H9) was described in 2017. Bat H9N2: high replication + transmission potential in ferrets, infects human lung explant cultures, is able to evade antiviral inhibition by MxA in transgenic B6 mice
Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2024.8755
And related is Vaccination of poultry against highly pathogenic avian influenza – part 1. Available vaccines and vaccination strategies
https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2023.8271
Immunogenicity and protective efficacy of a multivalent herpesvirus vectored vaccine against H9N2 low pathogenic avian influenza in chicken
https://www.sciencedirect.com/science/article/pii/S0264410X24004523
Building better vaccines for avian influenza: herpes vectored H9 & NDV vaccine. Vaccine elicited systemic NDV F- and AIV H9-specific antibodies but also local antibodies in eye wash fluid and oropharyngeal swabs. ???? viral load, doesn’t block infection
Highly Pathogenic Avian Influenza (HPAI) H5 Clade 2.3.4.4b Virus Infection in Birds and Mammals
https://www.preprints.org/manuscript/202404.1123/v1
Review of HPAI. Includes details on outcomes of many experimental studies which is useful. If comprehensive, demonstrates how few experimental studies on panzootic strain are available (although probably in the works).
Rapid mortality in captive bush dogs (Speothos venaticus) caused by influenza A of avian origin (H5N1) at a wildlife collection in the United Kingdom
https://www.biorxiv.org/content/10.1101/2024.04.18.590032v1.abstract
In 2022, an unusual mortality event of captive bush dogs with HPAI. Enclosure of 15 bush dogs, 10 died in 9 days w some dogs exhibiting neurological disease. Ingestion of infected meat.
Evaluating the epizootic and zoonotic threat of an H7N9 low pathogenicity avian influenza virus (LPAIV) variant associated with enhanced pathogenicity in turkeys
https://www.biorxiv.org/content/10.1101/2024.04.16.589776v1.abstract
Despite poultry vaccination, H7N9 has not been eradicated. HA Q217 is now dominant in China following vaccination, has affected antigenicitiy = can generate novel variants with increased risk via altered pathogenicity and potential HA antigenic escape.
Highly pathogenic avian influenza H5N1 virus infections of dairy cattle and livestock handlers in the United States of America
https://www.tandfonline.com/doi/full/10.1080/21505594.2024.2343931
Editorial summary of events.
The infectious disease trap of animal agriculture
https://www.science.org/doi/10.1126/sciadv.add6681
Its really critical for us to reflect upon the role of animal agriculture in emerging infection diseases and zoonotic diseases. Current HPAI panzootic result of endemicity of disease in poultry systems for decades.
Confirmation of Highly Pathogenic Avian Influenza (HPAI) H5N1 Associated with an Unexpected Mortality Event in South Polar Skuas (Stercorarius maccormicki) during 2023-2024 Surveillance Activities in Antarctica
https://www.biorxiv.org/content/10.1101/2024.04.10.588951v1
First mortality event in Antarctica (south of 60°S) by HPAI H5N1 was South polar skuas, 28 Feb 2024, James Ross Island. Would be great to see future sequencing results from this.
Sero-epidemiology of Highly Pathogenic Avian Influenza viruses among wild birds in subarctic intercontinental transition zones
https://www.researchsquare.com/article/rs-4233804/v1
Seroprevalence study for HPAI in Alaska, Iceland – important stepping stones to North America. Samples from 2010–2019, wild migratory seabirds & waterfowl. seroprev of HPAI = 7.3%, variability per year, more in Alaska vs Iceland.
Proteomics analysis of duck lung tissues in response to highly pathogenic avian influenza virus
https://www.preprints.org/manuscript/202404.0768/v1
In duck lungs, infection by HPAI (old 2011 strain), 2028 proteins differentially expressed. Activation of RIG-I-like receptor and Jak-STAT signaling pathways = induction of interferon stimulated gene expression = protective antiviral immune response
High-pathogenicity avian influenza in wildlife: a changing disease dynamic that is expanding in wild birds and having an increasing impact on a growing number of mammals
https://avmajournals-avma-org.eu1.proxy.openathens.net/view/journals/javma/aop/javma.24.01.0053/javma.24.01.0053.xml
Review of HPAI with a nice overview of what is happening mammals, including diversity affected, and disease signs outlined.
The virus is out of the barn: the emergence of HPAI as a pathogen of avian and mammalian wildlife around the globe
https://avmajournals.avma.org/view/journals/ajvr/aop/ajvr.24.01.0018/ajvr.24.01.0018.xml
Review on emergence of HPAI affecting avian and mammalian wildlife around the globe.
Genetic and virological characteristics of a reassortant avian influenza A H6N1 virus isolated from wild birds at a live-bird market in Egypt
https://link.springer.com/article/10.1007/s00705-024-06022-6
H6N1 caused human infection in Taiwan in 2013. Surveillance in Egyptian live bird markets, detected H6N1= replicated efficiently in mice w/o prior adaptation, + grew faster w higher titers tvs the ancestral strain.
Abundant Intra-Subtype Reassortment Revealed in H13N8 Influenza Viruses
https://www.mdpi.com/1999-4915/16/4/568
Lovely to see more H13 data, here from Republic of Buryatia. Viruses have no host data (but likely to be gulls), and have extensive reassortment, which aligns with work I did in 2011.
Influenza at the human-animal interface
https://cdn.who.int/media/docs/default-source/influenza/human-animal-interface-risk-assessments/influenza_summary_ira_ha_interface_march_2024.pdf
Avian Influenza Virus and Avian Paramyxoviruses in Wild Waterfowl of the Western Coast of the Caspian Sea (2017–2020)
https://www.mdpi.com/1999-4915/16/4/598
Outcome of influenza and paramyxo surveillance from western coast of Caspian Sea, 2017 to 2020. 1438 individuals of 26 species collected. 21 AIV strains, but no HPAI. 12 avian paramyxoviruses incl AMPV-1, AMPV-4, APMV-6.
Isolation and genetic characteristics of Novel H4N1 Avian Influenza viruses in ChongQing, China
https://virologyj.biomedcentral.com/articles/10.1186/s12985-024-02352-8
Hgh AIV prevalence in poultry market in China, with isolation of H4N1. Genome signs of reassortment between wild and domesticated waterfowl, multiple mutations and demonstrates potential for host transfer.
Stranding and Mass Mortality in Humboldt Penguins (Spheniscus humboldti), Associated to HPAIV H5N1 Outbreak in Chile.
https://www.sciencedirect.com/science/article/pii/S0167587724000928
January and August 2023, 2,788 Humbolt Penguins stranded/died = increase in mortality coinciding w introduction of HPAIV H5N1 in Chile. 2 sequences generated, do not belong to the same subcluster = evidencing independent introductions
Emerging Threats: Is Highly Pathogenic Avian Influenza A(H5N1) in Dairy Herds a Prelude to a New Pandemic?
https://www.sciencedirect.com/science/article/pii/S1477893924000358
This paper was written entirely independently from the people doing the work. Its always disappointing to see things like this in the literature.
Detection of clade 2.3.4.4b highly pathogenic H5N1 influenza virus in New York City
https://www.biorxiv.org/content/10.1101/2024.04.04.588061v1.abstract
HPAI is truly affecting animals everywhere. Surveillance in the urban environs of NY city, over 1700 samples collected and virus detected in 4 different bird species. Viruses w different genotypes. Important for risk, response, biosecurity
In Ovo Models to Predict Virulence of Highly Pathogenic Avian Influenza H5-Viruses for Chickens and Ducks
https://www.mdpi.com/1999-4915/16/4/563
The development of in ovo models to understand virulence of HPAI decreases time requried and cost (compared to IVPI). Remarkable differences in virulence observed bw poultry species, differences in replication rate, systemic virus dissemination
Drivers for a pandemic due to avian influenza and options for One Health mitigation measures
https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2024.8735
New from EFSA: guidance for public health authorities in EU/EEA countries on how to interpret the current situation of HPAI outbreaks in animals, includes mitigation measures. Useful guidance document.
Hemagglutinin and neuraminidase of an H7N7 non-pathogenic avian influenza virus coevolved during the acquisition of intranasal pathogenicity in chickens
https://www.researchsquare.com/article/rs-4161114/v1
To better be able to predict the emergence of HPAI, we need to clarify the steps in the process of LPAI to HPAI. Here, a low path virus with a polybasic cleavage site passaged to reveal required adaptations
Long-term co-circulation of multiple influenza A viruses in pigs, Guangxi, China
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2337673
Surveillance of pigs in 12 cities in China = 192 positive samples, 19 genomes. Eurasian avian-like H1N1 swIAVs (G4) still remained predominant in pig populations, and multiple novel H3N2 genotypes from N. A. triple reassotant present w matrix of H9N2. Somewhat concerning.
High pathogenic avian influenza A(H5) viruses of clade 2.3.4.4b in Europe – why trends of virus evolution are more difficult to predict
https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veae027/7641822
Incredible overview of HPAI in Europe. 1956 genomes, spatial temporal patterns of diffusion, reassortment galore, emergence of gull genotype, adaptive mutations. Tour de force!
Avian Influenza A (H5N1) Outbreak 2024 in Cambodia: Worries Over the Possible Spread of the Virus to Other Asian Nations and the Strategic Outlook for its Control
https://journals.sagepub.com/doi/full/10.1177/11786302241246453
Another paper wherein no one from Cambodia who is doing the work has been cited.
Evolution of H6N6 viruses in China between 2014 and 2019 involves multiple reassortment events
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2341142
H6 one of the most common LPAI subtypes in birds. From 2014-2019 168 viruses, 98 genotypes H6N6 in China, and isolates reassorted with six subtype viruses: H6N2, H5N6, H7N9, H5N2, H4N2, and H6N8, resulting in nine novel H6N6 reassortment events.
Avian influenza outbreaks in domestic cats: another reason to consider slaughter-free cell-cultured poultry?
https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1283361/full
This article debates that technology allowing the production of slaughter-free meat, including poultry, from cell and tissue cultures could be considered as a part of a mitigation strategy to decrease the overall burden and threat of adaptation of avian influenza viruses to human hosts
Naturally occurring highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b infection in three domestic cats in North America during 2023
https://www.sciencedirect.com/science/article/pii/S0021997523002499
Cats, like most predators/scavengers, have been repeatedly affected by HPAI. Here, three cats with neurological abnormalities. Pulmonary congestion + oedema, and cerebrocortical malacia w haemorrhage. 1 cat survived 10 days after onset of encephalitis.
Mammalian Adaptation Risk in HPAI H5N8: A Comprehensive Model Bridging Experimental Data with Mathematical Insights
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2339949
Here, mathematical models used to evaluate the growth, selection, and RNA load of eight recombinant viruses with mammalian adaptive markers + interplay. Revealed risk from adaptive markers of HPAI H5N8 in mammals.
Field and laboratory investigation of highly pathogenic avian influenza H5N6 and H5N8 in Quang Ninh province, Vietnam, 2020 to 2021
https://vetsci.org/DOIx.php?id=10.4142/jvs.23184
In Viet Nam, 38 cases H5N6 clade 2.3.4.4h viruses and 3H5N8 clade 2.3.4.4b viruses. Raising poultry in uncovered ponds, poultry traders visiting the farm, farms with 50 – >2000 birds associated with HPAI outbreaks.
Surveillance and Genetic Analysis of Low-Pathogenicity Avian Influenza Viruses Isolated from Feces of Wild Birds in Mongolia, 2021 to 2023
https://www.mdpi.com/2076-2615/14/7/1105
Intro of HPAI in Korea linked to reassortment in bird breeding areas. Here, 10,149 samples from wild birds in Mongolia, = 1.01% prevalence, 77 isolated strains. No HPAI, but link bw Mongolia and Korea. Great collab and big picture thinking!
Generation and characterization of a nanobody against the avian influenza virus H7 subtype
https://www.sciencedirect.com/science/article/abs/pii/S0141813024022633
A neutralising antibody with high HAI activity was enhanced via antiviral activity through oligomerization, = potential for developing effective agents for the prevention, diagnosis, and treatment of H7.
Evolutional dynamics of highly pathogenic avian influenza H5N8 genotypes in wintering bird habitats: Insights from South Korea’s 2020–2021 season
https://www.sciencedirect.com/science/article/pii/S2352771424000454
>7000 samples collected from S. Korea 2020-21, of which 5% positive for H5N8, predominantly from bird carcasses. Reassortment at play, with emergence of “G2” genotype. S. Korea a high density wintering area, a hot spot for potentially virus evolution.
HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS RESULTED IN UNPRECEDENTED REPRODUCTIVE FAILURE AND MOVEMENT BEHAVIOUR BY NORTHERN GANNETS
http://www.marineornithology.org/PDF/52_1/52_1_121-128.pdf
Following outbreak of HPAI in Gannet colonies in Newfoundland: reproductive success 17% = adults abandoning nests, many dying. Birds also had extremely long foraging trips, and inter-colony movement. In addition to HPAI, marine heatwave also occuring.
Wildlife under threat as avian influenza reaches Antarctica
https://www.woah.org/en/wildlife-under-threat-as-avian-influenza-reaches-antarctica/
Evolutionary Events Promoted Polymerase Activity of H13N8 Avian Influenza Virus
https://www.mdpi.com/1999-4915/16/3/329
H13’s are pretty weird, and almost exclusively found in gulls. Experimental reassortment of H13N8 with H9N2 viruses results in viruses with enhanced capacity for mammalian adaptations. Paper is a bit sensationalist, and I wonder about the ethics of the experiments performed.
Association of biosecurity and hygiene practices with avian influenza A/H5 and A/H9 virus infections in turkey farms
https://www.frontiersin.org/articles/10.3389/fvets.2024.1319618/full
24.68% of turkey samples in Bangladesh positive for AIV (5.95% H5, 6.81% H9). Presence of footbaths, absence of nearby poultry farms, concrete flooring, and avoidance of mixing newly purchased turkeys with existing stock reduce the risk.
TRIM21 Promotes Oxidative Stress and Ferroptosis through the SQSTM1-NRF2-KEAP1 Axis to Increase the Titers of H5N1 Highly Pathogenic Avian Influenza Virus
https://www.mdpi.com/1422-0067/25/6/3315
TRIM21 is involved in signal transduction and antiviral responses, and is upregulated during H5N1 infection in A549 cells, which alleviates oxidative stress and ferroptosis.
Avian influenza virus cross-infections as test case for pandemic preparedness: From epidemiological hazard models to sequence-based early viral warning systems
https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.14389
What can we learn from human infections with avian influenza for pandemic preparedness? CDC IRAT and WHO TIPRA risk models for risk prediction, and genomic sequence data useful. Mink farms and animal markets concerning. I actually struggled to find the point of this paper
Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9
https://www.cell.com/heliyon/fulltext/S2405-8440(24)04249-X
Twelve host proteins have intricate interactions with viral proteins to modulate HPAI H7N9, including 6 which appear unique to H7N9 (relative to other influenzas). Important in understand infections and outcomes.
Seroprevalence of Avian Influenza A(H5N6) Virus Infection, Guangdong Province, China, 2022
https://wwwnc.cdc.gov/eid/article/30/4/23-1226_article
While HPAI H5N1 is problematic globally, China struggled with H5N6: 86 human cases. Serology study in humans Jan-March 2022: 1 of >6000 humans positive, BUT poultry workers excluded. Low risk of human infection in general population, in alignment w WHO.
Upper Respiratory Tract Disease in a Dog Infected by a Highly Pathogenic Avian A/H5N1 Virus
https://www.mdpi.com/2076-2607/12/4/689
Remember the HPAI outbreak in cats in Poland in 2023? Well, a dog was also infected. Respiratory disease signs consistent with “kennel cough” – dog did not respond to initial treatment with antibiotics, later fluA test result positive.
Genetic Diversity of Avian Influenza Viruses Detected in Waterbirds in Northeast Italy Using Two Different Sampling Strategies
https://www.mdpi.com/2076-2615/14/7/1018
In Italy, across 2 sites, Aug 2021-April 2022: LPAI H5N3, H1N1, H9N2 detected, related to viruses in Black Sea/Mediterranean migratory flyway. No 2.3.4.4b HPAI detected
A Phase 2 Clinical Trial to Evaluate the Safety, Reactogenicity, and Immunogenicity of Different Prime-Boost Vaccination Schedules of 2013 and 2017 A(H7N9) Inactivated Influenza Virus Vaccines Administered with and without AS03 Adjuvant in Healthy US Adults
https://academic.oup.com/cid/advance-article-abstract/doi/10.1093/cid/ciae173/7636247
Lack of Highly Pathogenic Avian Influenza H5N1 in the South Shetland Islands in Antarctica, Early 2023
https://www.mdpi.com/2076-2615/14/7/1008
Great to see the publication of more HPAI surveillance in the Antarctic. Surveillance efforts in the South Shetland Islands in January 2023 all negative, consistent with observations that HPAI likely didnt reach the peninsula until later in the season.
Index case of H5N1 clade 2.3.4.4b highly pathogenic avian influenza virus in wild birds, South Korea, November 2023
https://www.frontiersin.org/articles/10.3389/fvets.2024.1366082/full
No pdf yet.
Comparison of the Clinical Manifestation of HPAI H5Nx in Different Poultry Types in the Netherlands, 2014–2022
https://www.mdpi.com/2076-0817/13/4/280
Important to remember that HPAI has different impacts on different poultry types, and that different clades have also had different impacts. Here, a careful comparison of H5N1, H5N8, H5N6, b/w 2014, 2018, 2020, 2022 for different poultry types and ages
Experimental Evaluation for the Dual Infection of Low Pathogenic Avian Influenza virus H9N2 and Escherichia Coli in H9N2 Immunized and Non-immunized Broiler Chickens
https://ejvs.journals.ekb.eg/article_347297.html
Significant mortality in chickens co-infected w H9N2 and E.coli (50-90% deaths) and severe clinical illness compared to influenza alone. Use of H9N2 vaccines can protect broilers from mortality + minimize shedding post-challenge.
Long-Distance Avian Migrants Fail to Bring 2.3.4.4b HPAI H5N1 Into Australia for a Second Year in a Row
https://onlinelibrary.wiley.com/doi/10.1111/irv.13281
Summary of our “enhanced surveillance” activities in 2023.
Avian influenza overview December 2023 – March 2024
https://www.efsa.europa.eu/en/efsajournal/pub/8754
Updated EFSA
Highly pathogenic avian influenza A(H5N1) virus of clade 2.3.4.4b isolated from a human case in Chile causes fatal disease and transmits between co-housed ferrets
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2332667
Human case of HPAI in Chile tested in ferrets to understand infection in mammals, and possibility of transmission. Virus transmitted to naïve contacts via direct contact but NOT via respiratory droplets or fomite transmission models. So, virus can transmit in “unnatural” setting, but cant transmit via airbourne transmission which is needed for effective mammal-to-mammal transmission.
Divergent Pathogenesis and Transmission of Highly Pathogenic Avian Influenza A(H5N1) in Swine
https://pubmed.ncbi.nlm.nih.gov/38478379/
Assessment of susceptibility of pigs to avian and mammalian HPAI H5N1 2.3.4.4b. All strains replicated in the lung of pigs w lesions consistent with influenza. But, viral replication in the nose + transmission only observed when using mammalian viruses.
Amino acids in the polymerase complex of shorebird-isolated H1N1 influenza virus impact replication and host-virus interactions in mammalian models
https://www.tandfonline.com/doi/abs/10.1080/22221751.2024.2332652
Diversity of LPAI in wild birds, zoonotic risk mostly uknown. H1N1 viruses in birds in US assessed. Viruses with key subs in the PB2 ????replication kinetics, ???? infectivity, ????polymerase complexes, ????replication kinetics. Pathogenicity in the mouse????.
Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9
https://www.cell.com/heliyon/pdf/S2405-8440(24)04249-X.pdf
Avian Influenza in Low and Middle-Income Countries (LMICs): Outbreaks, Vaccination Challenges and Economic Impact
http://www.pvj.com.pk/pdf-files/23-496.pdf
Role of the World Organisation for Animal Health in global wildlife disease surveillance
https://www.frontiersin.org/articles/10.3389/fvets.2024.1269530/
Overview of the role of WOAH, and surveillance data being shared. E.g. b/w 2019–2023, 154 countries reported 68862973 cases for 84 diseases. 150 countries reported 68672115 cases in domestic animals and 95 countries reported 190858 cases in wild animals.
Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022
https://www.sciencedirect.com/science/article/pii/S2095311924001278
H9N2 is most common subtype in poultry in China; is zoonotic. In Shanxi Province, across 14 viruses, 7 genotypes, 2 antigenic clusters? High transmission efficiency & diverse replication ability in chickens. Viruses replicate efficiently in mice lungs.
Natural Infection with H5N1 Highly Pathogenic Influenza (HPAI) Virus in 5- and 10-Day-Old Commercial Pekin Ducklings (Anas platyrhynchos domesticus)
https://doi.org/10.1637/aviandiseases-D-23-00067
No access
Assessment of Knowledge and Biosecurity Practices Related to Avian Influenza Among Poultry Workers in a District of South India
https://journals.lww.com/jphmp/abstract/9900/assessment_of_knowledge_and_biosecurity_practices.223.aspx
No access
Complex N-glycans are important for interspecies transmission of H7 influenza A viruses
https://journals.asm.org/doi/abs/10.1128/jvi.01941-23
HAs bind to glycan receptors with terminal sialic acids, which are either NeuAc or NeuGc (mainly found in horses and pigs but not in birds and humans). NeuGc-adapting mutations in avian H7 IAVs in vitro and in vivo. Affect viral replication in chicken cells, not duck cells, positively affect replication in horse cells. Mutations reduce virus virulence and mortality in chickens.
Control of highly pathogenic avian influenza through vaccination
https://www.sciencedirect.com/science/article/pii/S2095311924001163
China been using vaccines to control HPAI H5 since 2004, with more than 300 billion doses used by 2022. Paper outlines suggestions regarding requirements for vaccine selection and effectiveness. Should be taken with consideration, and some studies have conflicting conclusions with regards to effect of vaccines on virus evolution.
Recent H9N2 avian influenza virus lost hemagglutination activity due to a K141N substitution in hemagglutinin
https://journals.asm.org/doi/abs/10.1128/jvi.00248-24
Increasingly, H9N2 strains have a loss of hemagglutination activity at 37°C, = challenges for detection & monitoring = K141N in HA. N141K mutation impedes H9N2 ability to bind to receptors, enhances viral thermostability, reduces plaque size on MDCK
MDCK-Adaptive Mutation of A169S Changes Glycosylation Pattern of Hemagglutinin and Enhances MDCK-Based H7N9 Vaccine Virus Production without Loss of Antigenicity and Immunogenicity
https://www.mdpi.com/2076-393X/12/3/291
Adaptation of egg-derived H7N9 CVV in mammalian cell line is an approach to make high-growth virus for mass production of vaccine manufacturing. Investigation of mutations that arise in the process and impact on replication and vaccine responses.
Surveillance for highly pathogenic avian influenza A (H5N1) in a raptor rehabilitation center — 2022
https://conservancy.umn.edu/handle/11299/261581
No full text access
H5N1 high pathogenicity avian influenza virus in migratory birds exhibiting low pathogenicity in mallards increases its risk of transmission and spread in poultry
https://www.sciencedirect.com/science/article/abs/pii/S0378113524000609
HPAI virus from crane in china: highly pathogenic to chickens, moderately pathogenic to BALB/c mice, highly infectious but not lethal to mallards. Minor antigenic drift compared with the H5-Re14 vaccine strain (used in China).
Pacific and Atlantic sea lion mortality caused by highly pathogenic Avian Influenza A(H5N1) in South America
https://www.sciencedirect.com/science/article/pii/S1477893924000267
Good to see this pre-print now published. Excellent description of HPAI spread in sea lions of south america – down the pacific coast, and back up the Atlantic coast. Huge impact on these animals.
Genomic epidemiology of highly pathogenic avian influenza A (H5N1) virus in wild birds in South Korea during 2021–2022: Changes in viral epidemic patterns
https://academic.oup.com/ve/article/10/1/veae014/7606456
H5N1 high pathogenicity avian influenza virus in migratory birds exhibiting low pathogenicity in mallards increases its risk of transmission and spread in poultry
https://www.sciencedirect.com/science/article/abs/pii/S0378113524000609
2021–2022, 1 H5N8 + 43 H5N1 2.3.4.4b HPAI viruses in wild birds in South Korea. 5 genotypes. Wild birds play a vital role in viral transmission and long-term maintenance
Rapid adaptive substitution of L226Q in HA protein increases the pathogenicity of H9N2 viruses in mice
https://www.sciencedirect.com/science/article/pii/S2772431X24000042
More than 100 human cases due to H9N2 – all cases from China assayed in mice: low or no pathogenicity in mice, L226Q in HA rapidly emerged which was responsible for severe infections/fatalities. 226Q = a competitive advantage in mice.
Highly Pathogenic Avian Influenza A (H5N1) Suspected in penguins and shags on the Antarctic Peninsula and West Antarctic Coast
https://www.biorxiv.org/content/10.1101/2024.03.16.585360v1.full.pdf
Suspected HPAI in Adélie penguins and Antarctic shags. Detections at 2/13 sites; limited to Antarctic Peninsula.
Analysis of H5N8 influenza virus infection in chicken with mApple reporter genes in vivo and in vitro
https://www.sciencedirect.com/science/article/abs/pii/S0378113524000749
Labelled H5N8 viruses used to infect monocytes/macrophages in PBMCs – detected the mApple in 55.1%-80.4%, = chicken primary monocytic/macrophages are important target cells for avian influenza. Found in chicken lung CD45+ cells, CD4 + CD8 T cells.
Phylogeographic Dynamics of H9N2 Avian Influenza Viruses in Tunisia
https://pubmed.ncbi.nlm.nih.gov/38467378/
No access beyond pubmed?
Long distance avian migrants fail to bring 2.3.4.4b HPAI H5N1 into Australia for a second year in a row
https://www.biorxiv.org/content/10.1101/2024.03.06.583767v1
The summary of our enhanced surveillance program, targeted to incoming migratory birds to Australia is now out. We sampled ~ 1000 birds, with no indication of HPAI. Oceania last continent free from HPAI
Molecular characterization of avian influenza viruses (H5N2, H5N8, H5Nx and H9N2) isolated from chickens and ducks in the South of Egypt 2020 – 2021
https://www.advetresearch.com/index.php/AVR/article/view/1562
143 samples from chicken and duck farms in Egypt, 2020 w H5N2, H5N8, H5Nx, H9N2. 2.3.4.4b clade with close relation to H5N8 isolates from Egypt in 2021 and Kazakhstan in 2020.
Molecular detection of highly pathogenic avian influenza A (H5N8) virus isolated from domestic ducks and chickens in Egypt across 2018-2021
https://advetresearch.com/index.php/AVR/article/view/1670
Vaccinated duck and chicken flocks in 2018-2021 from different provinces, Egypt. Flocks with respiratory, nervous signs, with 90% mortality. Egyptian H5N8 viruses clustered in group B Russian like reassortant H5N8 viruses of clade 2.3.4.4
H5N1 high pathogenicity avian influenza virus in migratory birds exhibiting low pathogenicity in mallards increases its risk of transmission and spread in poultry
https://www.sciencedirect.com/science/article/abs/pii/S0378113524000609
2.3.4.4b in Grey Crane in China, w some segments similar to human cases. Substitutions for enhanced replication & mammalian pathogenicity. Highly pathogenic to chickens, moderately pathogenic to BALB/c mice, and infectious but not lethal to mallards. Some drift from current vaccine
Wild Bird-Origin H6N2 Influenza Virus Acquires Enhanced Pathogenicity after Single Passage in Mice
https://doi.org/10.3390/v16030357
H6 is very common in wild birds. Here a virus from a wild bird in China passaged in mice. Initially replication poor, but upon second passage 2 mutations. Retained the α-2, 3-linked sialic acid binding property and failed to transmit in guinea pigs. PB2 E627K enhanced polymerase activity and HA A110V decreased the pH of HA activation
Genetics of H5N1 and H5N8 High-Pathogenicity Avian Influenza Viruses Isolated in Japan in Winter 2021–2022
https://www.mdpi.com/1999-4915/16/3/358
In 2021–2022, H5N1 + H5N8 HPAI caused serious outbreaks in Japan: 25 at poultry farms + 107 in wild birds/environment. Three genetic groups, but at different locations/time. Strong links bw viruses in Japan and Siberia.
Monitoring avian influenza in mammals with real-time data
https://www.tandfonline.com/doi/full/10.1080/20477724.2024.2323843
Open access database https://github.com/fbranda/avian-mammals and a corresponding visualization https://tinyurl.com/avianflu-mammals-map designed to more easily monitor reported cases in mammals across various countries. Essentially just WAHIS data, but they are trying to make it easier to access.
The H9N2 avian influenza virus increases APEC adhesion to oviduct epithelia by viral NS1 protein-mediated activation of the TGF-β pathway
https://journals.asm.org/doi/full/10.1128/jvi.01512-23
H9N2 viruses enhance secondary APEC [E.coli] infection in chickens via enhancing fibronectin, which promotes bacterial adhesion. Viral NS1 transforms growth factor beta (TGF-β) signalling pathway.
Avian H6 Influenza Viruses in Vietnamese Live Bird Markets during 2018–2021
https://www.mdpi.com/1999-4915/16/3/367
Plenty of H6 viruses found in Vietnamese live bird markets. Most viruses highly similar, but reassortment present. Amino acid motif in HA confers binding to both avian- and human-type receptors on host cells
Genetic evolution analysis of hemagglutinin and neuraminidase genes of H9N2 avian influenza virus in external environment of some areas of Yunnan Province, China from 2020 to 2023
https://www.biorxiv.org/content/10.1101/2024.02.24.581849v1.abstract
HA and NA of H9N2 in Yunnan Province evolved continuously, but still Y280 clade. Ability to bind to the mammalian sialic acid α-2,6 sialic acid receptor. Small and descriptive study.
Highly Pathogenic Avian Influenza A(H5N1) Viruses from Multispecies Outbreak, Argentina, August 2023.
https://wwwnc.cdc.gov/eid/article/30/4/23-1725_article
HPAI from birds and marine mammals in Argentina: not related to first cases in Argentina, seperate introduction? 9 mutations in marine mammals viruses w Q591K and D701N in PB2 = mammalian adaptation mutations. pinniped-to-pinniped transmission?
Descriptive Epidemiology and Phylodynamics of the “First Wave” of an Outbreak of Highly Pathogenic Avian Influenza (H5N1 Clade 2.3.4.4b) in British Columbia and the Yukon, Canada, April to September 2022
https://www.hindawi.com/journals/tbed/2024/2327939/
Since November 2021, Canada experienced its longest and largest outbreak of HPAI in history. 21 wild bird species, 2 mammalian species, 4 commercial + 12 domestic small flocks. 5 genetic clusters, Eurasian+NAmerican segments
PB2 residue 473 contributes to the mammalian virulence of H7N9 avian influenza virus by modulating viral polymerase activity via ANP32A
https://journals.asm.org/doi/10.1128/jvi.01944-23
H7N9 caused >1000 human infections since 2013. Mutation at 473 and 627 in PB2 critical, but species-specific usage of ANP32A host factor affected mammalian adaption of AIV polymerase. PB2 473 novel viral host range determinant.
H7N6 highly pathogenic avian influenza in Mozambique, 2023
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2321993
H7N6 avian influenza outbreak in commercial layers in Mozambique. HPAI cleavage site PEPPKGPRFRR/GLF. Similar to viruses from South Africa in May 2023. Spread of HPAI viruses from South Africa, or an independant event in Mozambique?
Transmission restriction and genomic evolution co-shape the genetic diversity patterns of influenza A virus
https://www.sciencedirect.com/science/article/pii/S1995820X24000257
New tool to describe genotypes of influenza A viruses (all influenza A’s). Interpretation of results show lack of domain expertise, so wouldnt read into it too much.
Avian influenza virus circulation and immunity in a wild urban duck population prior to and during a highly pathogenic H5N1 outbreak
https://www.biorxiv.org/content/10.1101/2024.02.22.581693v1?rss=1
Sero-study of ducks in Newfoundland, demonstrating infection events across population in 2021 & 2022. Demonstrates clear power of serology in studying outbreaks, and that HPAI clearly spread through 100% of the ducks, twice = limited long term immunity?
Species-specific emergence of H7 highly pathogenic avian influenza virus is driven by intrahost selection differences between chickens and ducks
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011942
Co-innoculating HPAI and LPAI H7 in chickens and ducks leads to differential outcomes. HPAI selected for in chickens, LPAI selected in ducks. =HPAI selected intrahost, w species specific differences in tropism. Why HPAI H5 still selected in wildbirds?
N-Glycan Profiles of Neuraminidase from Avian Influenza Viruses
https://www.mdpi.com/1999-4915/16/2/190
Characterisation of N-glycans.
An algorithm for the characterization of influenza A viruses from various host species and environments
https://onlinelibrary.wiley.com/doi/10.1111/irv.13258
You have an influenza outbreak. Now what? Herein, a framework for influenza testing across environment, wildlife, livestock, humans.
Comparative examination of a rapid immunocytochemical test for the detection of highly pathogenic avian influenza virus in domestic birds, in field outbreaks.
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2320699
Diagnosing avian influenza using smears analysed with immunohistochemistry can be a useful, rapid approach when PCR diagnostics not available, but basic veterinary labs are.
Enhanced Downstream Processing for a Cell-Based Avian Influenza (H5N1) Vaccine
https://www.mdpi.com/2076-393X/12/2/138
There are still few approved HPAI vaccines on the market. Here, a two step downstream process as an efficient and cost-effective platform technology for cell-based H5N1 vaccines
Development of Virus-like Particle Plant-Based Vaccines against Avian H5 and H9 Influenza A Viruses
https://www.mdpi.com/2306-7381/11/2/93
Virus like particle vaccine for H5 and H9 developed. In mice, H5 VLP elicit robust antibody and Tcell response. Single dose of H5 VLP in chickens stimulated antibody response to neutralise virus infectivity.
Association of biosecurity and hygiene practices with Avian Influenza A/H5 and A/H9 virus infections in turkey farms
https://www.frontiersin.org/articles/10.3389/fvets.2024.1319618/abstract
No full text available yet.
Genetic insights of H9N2 avian influenza viruses circulating in Mali and phylogeographic patterns in Northern and Western Africa
https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veae011/7610485
H9N2 in Mali – G1 lineage similar to viruses in W & N Africa. Multiple molecular markers associated with an increased potential for zoonotic transmission and virulence, RSNR cleavage site. Likely arrived in Africa in 2015 via single introduction.
Pathological investigation of high pathogenicity avian influenza H5N8 in captive houbara bustards (Chlamydotis undulata), the United Arab Emirates 2020
https://www.nature.com/articles/s41598-024-54884-2
Outbreak of H5N8 in captive Houbara Bustards in UAE in 2020. Detailed pathology reveals hyperacute/acute forms exhibiting marked pantropism, endotheliotropism and neurotropism
Cross-Species Transmission Potential of H4 Avian Influenza Viruses in China: Epidemiological and Evolutionary Study
https://www.mdpi.com/1999-4915/16/3/353
H4s are one of the most common subtypes in wild birds. Over ~13 years, 31 isolates in Chinese poultry markets. Lots of genetic diversity , and some mutation of interest. Important not to lose sight of subtypes other than H5/H7/H9
Rapid loss of maternal immunity and increase in environmentally mediated antibody generation in urban gulls
https://www.nature.com/articles/s41598-024-54796-1
Antibodies across life stages: avian influenza antibodies present widely across all live stages of gulls, but maternal antibodies declined exponentially after hatching, but differences in nestling antibody levels due to parental effects.
Evaluation of different transport media for survival of H5N1 highly pathogenic avian influenza virus.
https://www.researchsquare.com/article/rs-3908312/v1
Sample collection methods have big impacts on laboratory analysis. Herein, various media tested for HPAI collection. They find that VTM not that great, but dont specify which VTM they used and how it was stored in the field. Also, stored at 37C before testing, which probably explains why VTM didnt do so well.
Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b in Domestic Ducks, Indonesia, 2022
https://wwwnc.cdc.gov/eid/article/30/3/23-0973_article
preprint now published – first report of 2.3.4.4b in Indonesian domestic ducks. 4430/5770 (76.8%) ducks died. From 2022-23, molecular surveillance didnt detect it further. As our nearest neighbour, high relevance to Australia.
Characterization of a human H3N8 influenza virus
https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(24)00069-0/fulltext
Characterisating of 2022 strain of H3N8 found in humans. Minor weight loss in ferrets, replicated efficiently in upper resp tract. Transmission via droplets occurred. SNPs in HA affecting receptor specificity. Antibodies found in some human sera
Caught Right on the Spot: Isolation and Characterization of Clade 2.3.4.4b H5N8 High Pathogenicity Avian Influenza Virus from a Common Pochard (Aythya ferina) Being Attacked by a Peregrine Falcon (Falco peregrinus)
https://meridian.allenpress.com/avian-diseases/article-abstract/doi/10.1637/aviandiseases-D-23-00062/499167/Caught-Right-on-the-Spot-Isolation-and?redirectedFrom=fulltext
No access
Spatio-temporal Dynamics and Risk Cluster Analysis of Highly Pathogenic Avian Influenza HPAI (H5N1) in Poultry: Advancing Outbreak Management through Customized Regional Strategies in Egypt
https://www.researchsquare.com/article/rs-3361650/v1
H5N1 is endemic in Egypt, detailed studies show shifting epidemiology. Menofia, important in early poultry impacts, but initial outbreaks didn’t originate there. Predominant hot spot region in rural villages, w fewer outbreaks in urbanized areas
An overlooked poultry trade network of the smallholder farms in the border provinces of Thailand, 2021: implications for avian influenza surveillance
https://www.frontiersin.org/articles/10.3389/fvets.2024.1301513/full
To understand virus transmission, we need to understand poultry movements. Here >300 poultry farmers and traders interviewed in Thailand. 99 subdistricts and 181 trade links with different in-degree and out-degree centralities
A new chromosome-scale duck genome shows a major histocompatibility complex with several expanded multigene families
https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-024-01817-0
We still have a long way to go to understand the duck immune response. Here, a chromosome scale duck genome assembly, with a complete genomic map of MHC. Gene arrangement primordial, but some expansions. Useful resource!
First confirmed cases of HPAI on the Antarctic Peninsula
https://ciencia.gob.es/Noticias/2024/febrero/gripe-aviar-antartida.html
A pan-influenza monoclonal antibody neutralizes H5 strains and prophylactically protects through intranasal administration
https://www.nature.com/articles/s41598-024-53049-5
Human therapuetics in case widespread HPAI in humans. An epitope on stem domain of H5 HA is highly conserved and the human monoclonal antibody CR9114 potently neutralizes all H5 viruses, even in the rare case of substitutions in its epitope.
Spatiotemporal genotype replacement of H5N8 avian influenza viruses contributed to H5N1 emergence in 2021/2022 panzootic
https://journals.asm.org/doi/abs/10.1128/jvi.01401-23
The dominant genotype replacement of the H5N8 viruses in 2020 contributed to the H5N1 outbreak in the 2021/2022 wave. Temporal–spatial coincidence bw the outbreak of H5N8 G1 virus and autumn migration may have expanded the H5 viral spread
Applied Research Note: Development and Validation of a Highly Specific Polyclonal Antibody Targeting Neuraminidase of Novel H3N8 Avian Influenza Virus
https://www.sciencedirect.com/science/article/pii/S1056617124000187
Are all avian influenza outbreaks in poultry the same? The predicted impact of poultry species and virus subtype
https://onlinelibrary.wiley.com/doi/full/10.1111/zph.13116
Are all influenza outbreaks on poultry farms the same?Simulations predicted large differences in the duration and severity of outbreaks, depending on the virus subtypes (only H7 and H7), outbreaks of HPAI shorter duration than LPAI
High pathogenicity avian influenza (HPAI) in the UK and Europe
https://assets.publishing.service.gov.uk/media/65d5fade2197b2001d7fa79e/highly-pathogenic-avian-influenza-europe-240216.pdf
Highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b from Peru forms a monophyletic group with Chilean isolates in South America
https://www.nature.com/articles/s41598-024-54072-2
More work to understand HPAI introduction and spread in South America. Critically, more evidence that events in Chile and Peru were due to same virus, falling to a monophyletic clade.
Protection conferred by an H5 DNA vaccine against highly pathogenic avian influenza in chickens: The effect of vaccination schedules
https://www.sciencedirect.com/science/article/pii/S0264410X23014226
DNA vaccine with A/gyrfalcon(2.3.4.4c) showed good protection in chickens infected with 2.3.4.4b with or without adjuvant, but with 2 doses. Also, good humoral immunity in birds 18 – 25 weeks old.
Genetic insertion of mouse Myxovirus-resistance gene 1 increases innate resistance against both high and low pathogenic avian influenza virus by significantly decreasing replication in chicken DF1 cell line
https://www.biorxiv.org/content/10.1101/2024.02.12.579928v1.abstract
Introduction of mouse Mx (which has strong antiviral acivity) to chicken cells efficiently reduced avian influenza infection load and less CPE. Foundation for gene editing of chickens to help resist HPAI?
Recent Changes in Patterns of Mammal Infection with Highly Pathogenic Avian Influenza A(H5N1) Virus Worldwide
https://wwwnc.cdc.gov/eid/article/30/3/23-1098_article
Excellent overview of impacts of HPAI on mammals. Big skew towards carnivores & scavengers (not surprising if we imagine lots of bird carcasses in the environment). SeaLions and Elephant Seals really raise questions of mammal-to-mammal transmission.
Genetic Characterization and Phylogeographic Analysis of the First H13N6 Avian Influenza Virus Isolated from Vega Gull in South Korea
https://www.mdpi.com/1999-4915/16/2/285
Another H13 genome, this time from a gull in South Korea. Sequences largely fall into gull-associated lineages, and phylogeographic origin is largely Eurasian, but with some exceptions. Certainly more work to do in gulls.
Genome sequences of hemagglutinin cleavage site predict the pathogenicity phenotype of avian influenza virus: statistically validated data for facilitating rapid declarations and reducing reliance on in vivo testing
https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2317430
Whether an avian influenza virus is HPAI or LPAI is usually confirmed using IVPI ($$, ethics, time). New study shows statistically robust association with cleavage site sequence and pathogenicity. Great resource!
Avian Influenza: a major threat to our struggling seabirds
https://www.rspb.org.uk/birds-and-wildlife/seabird-surveys-project-report
The data is horrific. 25% decline in Gannets (at least half of all NOrthern Gannets are found in the UK). 76% decline of breeding numbers of Great Skua in Scotland (60% of all great skuas are found in scotland)
Synchrony of Bird Migration with Global Dispersal of Avian Influenza Reveals Exposed Bird Orders
https://www.nature.com/articles/s41467-024-45462-1
Great to see this published. How 2.3.4.4b has spread amongst different region and species (in Eurasia): synchrony b/w bird migration and virus lineage movement, and differing bird orders at origins and destinations, including accipitriformes.
Novel H10N3 avian influenza viruses: a potential threat to public health
https://www.thelancet.com/journals/lanmic/article/PIIS2666-5247(23)00409-3/fulltext
H10 viruses, particularly in Asia, remain concerning. H10N3 found in 41 year old man in China. These H10 viruses also found in poultry markets (2020-22). Human & poultry viruses bind sialic acid-α-2,6-galactose receptors, poultry viruses lethal to mice
HPAIV outbreak triggers short-term colony connectivity in a seabird metapopulation
https://www.nature.com/articles/s41598-024-53550-x
Now published. During HPAI outbreaks in gannet colonies, GPS tracked individuals nstigated long-distance movements beyond well-documented previous ranges and prospected other colonies. Facilitated spread?
A systematic review of influenza virus in water environments across human, poultry, and wild bird habitats
https://www.sciencedirect.com/science/article/pii/S2589914723000464
Meta-analysis assess influenza in the environment including waste water. Features influenza A and B, and LPAI and HPAI. Really lacking any details that would make it useful.
Detection of clade 2.3.4.4 highly pathogenic avian influenza H5 viruses in healthy wild birds in the Hadeji-Nguru wetland, Nigeria 2022
https://onlinelibrary.wiley.com/doi/10.1111/irv.13254
Diversity of influenza viruses in wild birds in Nigeria in 2022, including in clinically healthy wild birds (from Jacanas to nighjars to ducks). E627K is present in some. 2.3.4.4b, but different from HPAI in Nigerian poultry in 2021.
Recombinant parainfluenza virus 5 expressing clade 2.3.4.4b H5 hemagglutinin protein confers broad protection against H5Ny influenza viruses
https://journals.asm.org/doi/10.1128/jvi.01129-23
Interrogation of potential HPAI vaccine – parainfluenza virus 5 -based vaccine candidate expressing 2.3.4.4b H5 HA. Intranasal immunization in ferrets stimulated antibody responses in mice, provide sterile immunity in mice and ferrets
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022
https://journals.asm.org/doi/10.1128/spectrum.03736-23
November 2022, HPAI caused an outbreak in a zoological park in the south of France, with the death of a Tibetan black bear and several bird species. PB2 E627K mutation in minute quantities in the gull, whereas it predominated in the bear
Incursion of Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus, Brazil, 2023
https://wwwnc.cdc.gov/eid/article/30/3/23-1157_article
HPAI in Royal terns and Cabot’s terns in Brazil, June 2023. Phylodynamics suggests incursion from Chile, although noting long branch lengths (missings sequences)
Comparative analysis and prediction of avian influenza in Shangrao city, China from 2016 to 2022
https://www.sciencedirect.com/science/article/abs/pii/S0042682224000163
Relationship between vaccination, COVID-19 pandemic, and bird migration on avian influenza in Shangrao City. Of concern is highly prevant H9, and increase of H5. Migratory birds and the COVID-19 pandemic have led to an increase in H9 subtype positivity. Take with a grain of salt.
Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus
https://www.mdpi.com/2076-393X/12/2/143
Recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against HPAI with suitable immunogenicity and protective efficacy.
A highly pathogenic avian influenza virus H5N1 clade 2.3.4.4 detected in Samara Oblast, Russian Federation
https://www.frontiersin.org/articles/10.3389/fvets.2024.1244430/full
Good to see some data from Russia, an important breeding area for migratory birds across Eurasia and Africa. A 2.3.4.4b virus found in a teal in Samara Oblast was related to field isolates from Russia, Nigeria, Bangladesh, and Benin
Bivalent Hemagglutinin Cleavage-Site Peptide Vaccines Protect Chickens from Lethal Infections with Highly Pathogenic H5N1 and H5N6 Avian Influenza Viruses
https://www.imrpress.com/journal/FBL/29/2/10.31083/j.fbl2902061/htm
Bivalent peptide vaccines containing H5 cleavage sites of viruses from both 2.3.4.4 H5N6 and clade 1 H5N1 designed to protect chickens from both H5N1 and H5N6 avian influenza viruses. immunised chickens were protected with no shedding in OP or C.
Guidance for reporting 2023 laboratory data on avian influenza
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/sp.efsa.2024.EN-8629
Enzootic Circulation, Massive Gull Mortality and Poultry Outbreaks during the 2022/2023 High-Pathogenicity Avian Influenza H5N1 Season in the Czech Republic
https://www.mdpi.com/1999-4915/16/2/221
Massive gull mortality and poultry outbreaks in Czech Republic 2022/23. Almost 1 million birds culled. Four HPAI genotypes, with BB mainly in gulls, and in turkey outbreak.
Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals
https://www.science.org/doi/full/10.1126/sciadv.adj4163
Species-specific differences in the host factor ANP32A determine the restriction of avian-signature polymerase in mammalian cells. ANP32 proteins incorporated into viral particles through combo w polymerase>transferred to cells> support replication. vian ANP32A (avANP32A) delivered by avian influenza A virions primes early viral replication in mammalian cells, thereby favoring the downstream interspecies transmission event by increasing the total amount of virus carrying adaptive mutations.
Genetic and Biological Characteristics of Duck-Origin H4N6 Avian Influenza Virus Isolated in China in 2022
https://www.mdpi.com/1999-4915/16/2/207
In 2022, samples from duck farms at Poyang Lake, China, with 3 H4N6 viruses isolated. Dual receptor binding properties and replicate efficiently not only in avian cells but also in mammalian cells. Viruses could infect mice without prior adaptation.
Emergence of a Novel Reassortant H5N6 Highly Pathogenic Avian Influenza Virus of Clade 2.3.2.1c from domestic poultry in China
https://essopenarchive.org/doi/full/10.22541/au.170665082.26537579
Detection of clade 2.3.2.1 H5N6 virus in China, against expectation of 2.3.4.4. Result of complex pattern of reassortment among clade 2.3.2.1 and clade 2.3.4.4 H5 and H6N6.
In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus
https://www.biorxiv.org/content/10.1101/2024.01.29.577768v1.abstract
NS1 does not play a role in the virulence or replication of HPAIV H7N1 in turkeys, illustrating genetic determinants of HPAIV between turkeys and chickens.
Host determination role of some amino acid sequences in the receptor-binding site and phylogenetic analysis of a high pathogenic Avian Influenza (H5N1) viruses isolated from Northern Turkey
https://www.authorea.com/users/470420/articles/712817-host-determination-role-of-some-amino-acid-sequences-in-the-receptor-binding-site-and-phylogenetic-analysis-of-a-high-pathogenic-avian-influenza-h5n1-viruses-isolated-from-northern-turkey
Retrospective study of HPAI in Turkey in 2006. Clade 2.2 and clade 2.2.1 and were closely related to European and Asian isolates
Detection of highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b in great skuas in Great Britain
https://www.techrxiv.org/doi/full/10.22541/au.170670066.67459333
Great summary of pathology of Great Skuas which died due to HPAI in Scotland.
Considerations for emergency vaccination of wild birds against high pathogenicity avian influenza in specific situations
https://www.woah.org/en/document/considerations-for-emergency-vaccination-of-wild-birds-against-high-pathogenicity-avian-influenza-in-specific-situations/
Transient RNA structures underlie highly pathogenic avian influenza virus genesis
https://www.biorxiv.org/content/10.1101/2024.01.11.574333v1
The contribution of individual characteristics of Anas and Aythya individuals to their susceptibility to low‐pathogenic avian influenza viruses in the south of Western Siberia
https://www.researchgate.net/publication/377366536_The_contribution_of_individual_characteristics_of_Anas_and_Aythya_individuals_to_their_susceptibility_to_low-pathogenic_avian_influenza_viruses_in_the_south_of_Western_Siberia/references
“Smart markets”: harnessing the potential of new technologies for endemic and emerging infectious disease surveillance in traditional food markets
https://journals.asm.org/doi/epub/10.1128/jvi.01683-23
Veterinarians’ knowledge and experience of avian influenza and perspectives on control measures in the UK
https://bvajournals.onlinelibrary.wiley.com/doi/10.1002/vetr.3713
Genetic properties and pathogenicity of a novel reassortant H10N5 influenza virus from wild birds
https://link.springer.com/article/10.1007/s00705-017-3234-3
Transmission dynamics and pathogenesis differ between pheasants and partridges infected with clade 2.3.4.4b H5N8 and H5N1 high-pathogenicity avian influenza viruses
https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001946
Complete Genome Sequence of an H10N5 Avian Influenza Virus Isolated from Pigs in Central China
https://journals.asm.org/doi/10.1128/jvi.02687-12
New Patterns for Highly Pathogenic Avian Influenza and Adjustment of Prevention, Control and Surveillance Strategies: The Example of France
https://www.mdpi.com/1999-4915/16/1/101
Targeted genomic sequencing of avian influenza viruses in wetland sediment from wild bird habitats
https://journals.asm.org/doi/10.1128/aem.00842-23
Markets as drivers of selection for highly virulent poultry pathogens
https://www.nature.com/articles/s41467-024-44777-3
Molecular evolution of avian influenza A (H9N2) virus in external environment in Anhui, 2019-2021
http://www.jbjc.org/cn/article/doi/10.3784/jbjc.202303150100
Public Health Implications of Antimicrobial Resistance in Wildlife at the One Health Interface
https://www.mdpi.com/2673-9992/25/1/1
Differing Expression and Potential Immunological Role of C-Type Lectin Receptors of Two Different Chicken Breeds against Low Pathogenic H9N2 Avian Influenza Virus
https://www.mdpi.com/2076-0817/13/1/95
Detection of Influenza A viruses and Avian H5 Subtype using a triplex qRT-PCR assay on the ABI Quantstudio 7 PCR system
https://www.protocols.io/view/detection-of-influenza-a-viruses-and-avian-h5-subt-crdjv24n.html
Multifaceted analysis of temporal and spatial distribution and risk factors of global poultry HPAI-H5N1, 2005-2023
https://www.sciencedirect.com/science/article/pii/S1751731124000168
Farm biosecurity practices affecting avian influenza virus circulation in commercial chicken farms in Bangladesh
https://www.sciencedirect.com/science/article/pii/S2352771424000077
Genetic Analysis of H5N1 High-Pathogenicity Avian Influenza Virus following a Mass Mortality Event in Wild Geese on the Solway Firth
https://www.mdpi.com/2076-0817/13/1/83
Avian influenza virus cross-infections as test case for pandemic preparedness: From epidemiological hazard models to sequence-based early viral warning systems
https://ami-journals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.14389
Emergence of a triple reassortment avian influenza virus (A/H5N6) from wild birds
https://www.journalofinfection.com/article/S0163-4453(24)00024-0/fulltext
Differential Protection of Chickens against Highly Pathogenic H5 Avian Influenza Virus Using Polybasic Amino Acids with H5 Cleavage Peptide
https://www.imrpress.com/journal/FBL/29/1/10.31083/j.fbl2901011/htm
Geographic, ecological, and temporal patterns of seabird mortality during the 2022 HPAI H5N1 outbreak on the island of Newfoundland
https://www.biorxiv.org/content/10.1101/2024.01.17.575746v1.abstract
Transboundary Determinants of Avian Zoonotic Infectious Diseases: Challenges for Strengthening Research Capacity and Connecting Surveillance Networks
https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341842/abstract
The risks and consequences of a high pathogenicity avian influenza outbreak in Aotearoa New Zealand
https://www.tandfonline.com/doi/full/10.1080/00480169.2023.2294915
A Comprehensive Analysis of H5N1 Evolution: Phylogenetic Insights and Emerging Mutations in Turkey’s Avian Influenza Landscape
https://www.researchsquare.com/article/rs-3831007/v1
Running the gauntlet; flyway-wide patterns of pollutant exposure in blood of migratory shorebirds
https://www.sciencedirect.com/science/article/abs/pii/S0013935124000276
Lesions and viral antigen distribution in bald eagles, red-tailed hawks, and great horned owls naturally infected with H5N1 clade 2.3.4.4b highly pathogenic avian influenza virus
https://journals.sagepub.com/doi/abs/10.1177/03009858231222227
A case-control study of the infection risk of H5N8 highly pathogenic avian influenza in Japan during the winter of 2020–2021
https://www.sciencedirect.com/science/article/abs/pii/S0034528824000158
Wild bird mass mortalities in eastern Canada associated with the Highly Pathogenic Avian Influenza A(H5N1) virus, 2022
https://www.biorxiv.org/content/10.1101/2024.01.05.574233v1.abstract
Efficacy of an inactivated influenza vaccine adjuvanted with Toll-like receptor ligands against transmission of H9N2 avian influenza virus in chickens
https://www.sciencedirect.com/science/article/abs/pii/S0165242724000011
Simultaneous Differential Detection of H5, H7, H9 and Nine NA Subtypes of Avian Influenza Viruses via a GeXP Assay
https://www.mdpi.com/2076-2607/12/1/143
Evolution and Spread of Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Wild Birds, South Korea, 2022-2023.
https://europepmc.org/article/med/38215495
HA N193D substitution in the HPAI H5N1 virus alters receptor binding affinity and enhances virulence in mammalian hosts
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2302854
Catastrophic mortality of southern elephant seals caused by H5N1 avian influenza
https://doi.org/10.1111/mms
Wild bird mass mortalities in eastern Canada associated with the Highly Pathogenic Avian Influenza A(H5N1) virus, 2022
https://www.biorxiv.org/content/10.1101/2024.01.05.574233v1
Evolution and biological characteristics of the circulated H8N4 avian influenza viruses
https://www.sciencedirect.com/science/article/pii/S209531192300480X
Meta-analysis of RNA Seq Datasets in Duck Lungs Infected with Highly Pathogenic Avian Influenza Viruses
https://www.indianjournals.com/ijor.aspx?target=ijor:jar&volume=13&issue=4&article=004
Comprehensive genome‑wide analysis of the chicken heat shock protein family: identification, genomic organization, and expression profiles in indigenous chicken with highly pathogenic avian influenza infection
https://link.springer.com/article/10.1186/s12864-023-09908-y
Expression of influenza A virus glycan receptor candidates in mallard, chicken, and tufted duck
https://academic.oup.com/glycob/advance-article/doi/10.1093/glycob/cwad098/7486526
Mortality in Sea Lions is associated with the introduction of the H5N1 clade 2.3.4.4b virus in Brazil, October 2023: Whole genome sequencing and phylogenetic analysis
https://www.researchsquare.com/article/rs-3793926/v1
Avian Influenza A(H5N1) Neuraminidase Inhibition Antibodies in Healthy Adults after Exposure to Influenza A(H1N1)pdm09
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10756388/
Neu5Gc binding loss of subtype H7 influenza A virus facilitates adaptation to gallinaceous poultry following transmission from waterbirds but restricts spillback
https://www.biorxiv.org/content/10.1101/2024.01.02.573990v1.abstract
H5N1 avian influenza virus PB2 antagonizes duck IFN-β signaling pathway by targeting mitochondrial antiviral signaling protein
https://www.sciencedirect.com/science/article/pii/S2095311923004872
The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-023-01257-8
Identification of specific neutralising antibodies for highly pathogenic avian influenza H5 2.3.4.4b clades to facilitate vaccine design and therapeutics
https://www.tandfonline.com/doi/full/10.1080/22221751.2024.2302106
Human behaviors driving disease emergence
https://onlinelibrary.wiley.com/doi/epdf/10.1002/evan.22015
A recent sovon report indicates that high path. avian #influenza has killed a high percentage of some bird populations in the #Netherlands. Sandwich tern, summer 2022: up to 56% estimated mortality. Peregrine falcon, winter 2021/2022: up to 56% estimated mortality.https://x.com/thijskuiken/status/1742922644864000491?s=20
Expression of influenza A virus glycan receptor candidates in mallard, chicken, and tufted duck
https://academic.oup.com/glycob/advance-article/doi/10.1093/glycob/cwad098/7486526
Highly pathogenic H5n1 avian influenza in free-living griffon vultures
https://digital.csic.es/handle/10261/341041
Trends and Spatiotemporal Patterns of Avian Influenza Outbreaks in Italy: A Data-Driven Approach
https://www.mdpi.com/2036-7449/16/1/1
Association of poultry vaccination with the interspecies transmission and molecular evolution of H5 subtype avian influenza virus
https://www.biorxiv.org/content/10.1101/2023.12.20.572711v1
Avian influenza overview September–December 2023
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/j.efsa.2023.8539
Antigenic Architecture of the H7N2 Influenza Virus Hemagglutinin Belonging to the North American Lineage
https://www.researchgate.net/publication/376802173_Antigenic_Architecture_of_the_H7N2_Influenza_Virus_Hemagglutinin_Belonging_to_the_North_American_Lineage/references
Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022
https://journals.sagepub.com/doi/10.1177/03009858231217224
The risk of highly pathogenic avian influenza in the Southern Ocean: a practical guide for operators and scientists interacting with wildlife
https://www.cambridge.org/core/journals/antarctic-science/article/risk-of-highly-pathogenic-avian-influenza-in-the-southern-ocean-a-practical-guide-for-operators-and-scientists-interacting-with-wildlife/6AC82C37924D61552C96D3BF61510F5A
Continued expansion of high pathogenicity avian influenza H5 in wildlife in South America and incursion into the Antarctic region
https://www.offlu.org/wp-content/uploads/2023/12/OFFLU-wildlife-statement-no.-II.pdf
Active surveillance for influenza virus and coronavirus infection in Antarctic birds and mammals in environmental fecal samples, South Shetland Islands
https://www.scielo.br/j/aabc/a/SBM8z7V9VhkrrCtV3tQpt4F/?lang=en
Detection and Phylogenetic Analysis of Contemporary H14N2 Avian Influenza A Virus in Domestic Ducks in Southeast Asia (Cambodia)
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2297552
Environmental Surveillance and Detection of Infectious Highly Pathogenic Avian Influenza Virus in Iowa Wetlands
https://pubs.acs.org/doi/10.1021/acs.estlett.3c00668
A naturally occurring HA-stabilizing amino acid (HA1-Y17) in an A(H9N2) low-pathogenic influenza virus contributes to airborne transmission
https://journals.asm.org/doi/10.1128/mbio.02957-23
Development and application of a triplex real-time PCR assay for the detection of H3, H4, and H5 subtypes of avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0032579123008532
Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice
https://www.sciencedirect.com/science/article/pii/S1995820X23001554
Efficacy of recombinant H5 vaccines delivered in ovo or day of age in commercial broilers against the 2015 U.S. H5N2 clade 2.3.4.4c highly pathogenic avian Influenza virus
https://virologyj.biomedcentral.com/articles/10.1186/s12985-023-02254-1
Highly pathogenic avian influenza H5N1 virus infection of companion animals
https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2289780
Virulence and transmission characteristics of clade 2.3.4.4b H5N6 subtype avian influenza viruses possessing different internal gene constellations
https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2250065
Characterization of Highly Pathogenic Avian Influenza A (H5N1) Viruses isolated from Cats in South Korea, 2023
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2290835
Low Pathogenic Avian Influenza H9N2 Viruses in Morocco: Antigenic and Molecular Evolution from 2021 to 2023
https://www.mdpi.com/1999-4915/15/12/2355
Genetically Related Avian Influenza H7N9 Viruses Exhibit Different Pathogenicity in Mice
https://www.mdpi.com/2076-2615/13/23/3680
First Report of Low Pathogenic Avian Influenza Subtype H9N2 in African Houbara Bustards (Chlamydotis undulata undulata) and Gamebirds in Morocco: Clinico-Pathological Findings, Molecular Characterization, and Associated Coinfections
https://www.mdpi.com/1999-4915/15/12/2374
Highly pathogenic avian influenza: Unprecedented outbreaks in Canadian wildlife and domestic poultry
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697099/
Highly Pathogenic Avian Influenza and its Complex Patterns of Reassortment
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4655071
Responding to avian influenza A H5N1 detection on a hospital property in Maine—An interdisciplinary approach
https://onlinelibrary.wiley.com/doi/full/10.1111/zph.13097
Author response: Global mapping of highly pathogenic avian influenza H5N1 and H5Nx clade 2.3.4.4 viruses with spatial cross-validation
https://folia.unifr.ch/global/documents/282060
Molecular modeling and phylogenetic analyses highlight the role of amino acid 347 of the N1 subtype neuraminidase in influenza virus host range and interspecies adaptation
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1309156/abstract
Mapping genetic markers associated with antigenicity and host range in H9N2 Influenza A viruses infecting poultry in Pakistan
https://doi.org/10.1637/aviandiseases-D-23-00029
Avian influenza viruses in wild birds in Canada following incursions of highly pathogenic H5N1 virus from Eurasia in 2021/2022
https://www.biorxiv.org/content/10.1101/2023.11.23.565566v1.abstract
Detection of Clade 2.3.4.4 Highly Pathogenic Avian Influenza H5 Viruses in Healthy Wild Birds in the Hadeji-Nguru Wetland, Nigeria, 2022
https://www.authorea.com/doi/full/10.22541/au.170055188.85169956
Comparative evolution of influenza A virus H1 and H3 head and stalk domains across host species
https://journals.asm.org/doi/10.1128/mbio.02649-23
Baloxavir marboxil use for critical human infection of avian influenza A H5N6 virus
https://www.cell.com/med/fulltext/S2666-6340(23)00361-6
H7 influenza A viruses bind sialyl-LewisX, a potential intermediate receptor between species
https://www.biorxiv.org/content/10.1101/2023.12.15.571923v1
Connectivity of marine predators over the Patagonian Shelf during the highly pathogenic avian influenza (HPAI) outbreak
https://www.biorxiv.org/content/10.1101/2023.12.12.570574v1
A broad antibody class engages the influenza virus hemagglutinin head at its stem interface
https://www.biorxiv.org/content/10.1101/2023.12.13.571543v1
Rapid detection of H5 subtype avian influenza virus using CRISPR Cas13a based-lateral flow dipstick
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1283210/full
Recombinant A(H6N1)-H274Y avian influenza virus with dual drug resistance does not require permissive mutations to retain the replicative fitness in vitro and in ovo
https://www.sciencedirect.com/science/article/pii/S0042682223002738
Low Pathogenic Avian Influenza H9N2 Viruses in Morocco: Antigenic and Molecular Evolution from 2021 to 2023
https://www.mdpi.com/1999-4915/15/12/2355
Effect of 2020–21 and 2021–22 Highly Pathogenic Avian Influenza H5 Epidemics on Wild Birds, the Netherlands
https://wwwnc.cdc.gov/eid/article/30/1/23-0970_article
Native and invasive bird interactions increase the spread of Newcastle disease in urban environments
https://link.springer.com/article/10.1007/s10530-023-03213-1
Epidemiological Disclosing and Molecular Subtyping for the Highly Pathogenic Avian Influenza Viruses H5N8 in Commercial Broilers and Layer Chickens in some Egyptian Governorates
https://ejvs.journals.ekb.eg/article_329440.html
Utility of Feathers for Avian Influenza Virus Detection in Commercial Poultry
https://www.mdpi.com/2076-0817/12/12/1425
Effect of avian influenza scare on transmission of zoonotic avian influenza: A case study of influenza A (H7N9)
https://www.sciencedirect.com/science/article/abs/pii/S0025556423001657
Highly pathogenic avian influenza H5N1 virus infection of companion animals
https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2289780
Metagenomic and Molecular Detection of Novel Fecal Viruses in Free-Ranging Agile Wallabies
https://link.springer.com/article/10.1007/s10393-023-01659-2
Different routes of infection of H5N1 lead to changes in infecting time
https://www.sciencedirect.com/science/article/abs/pii/S0025556423001694
A multiplex qRT-PCR assay for detection of Influenza A and H5 subtype targeting new SNPs present in high pathogenicity avian influenza Canadian 2022 outbreak strains
https://www.medrxiv.org/content/10.1101/2023.12.13.23298992v1
Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice
https://www.sciencedirect.com/science/article/pii/S1995820X23001554
Pigs are highly susceptible to but do not transmit mink-derived highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b
https://www.biorxiv.org/content/10.1101/2023.12.13.571575v1.abstract
Efficacy of recombinant H5 vaccines delivered in ovo or day of age in commercial broilers against the 2015 U.S. H5N2 clade 2.3.4.4c highly pathogenic avian Influenza virus
https://virologyj.biomedcentral.com/articles/10.1186/s12985-023-02254-1
Highly pathogenic avian influenza H5N1 virus infections in pinnipeds and seabirds in Uruguay: a paradigm shift to virus transmission in South America
https://www.biorxiv.org/content/10.1101/2023.12.14.571746v1.abstract
Annual report on surveillance for avian influenza in poultry and wild birds in Member States of the European Union in 2022
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10719745/
Has avian influenza virus H9 originated from a bat source?
https://www.frontiersin.org/articles/10.3389/fvets.2023.1332886/abstract
Unraveling molecular basis for reduced neuraminidase inhibitors susceptibility in highly pathogenic avian influenza A (H5N1) viruses isolated from chickens in India
https://www.biorxiv.org/content/10.1101/2023.12.15.571865v1.abstract
Survey of exposure to stranded dolphins in Japan to investigate an outbreak of suspected infection with highly pathogenic avian influenza (H5N1) clade 2.3.4.4(b) in humans
https://www.sciencedirect.com/science/article/pii/S2052297523001336
Identification of Pre-Emptive Biosecurity Zone Areas for Highly Pathogenic Avian Influenza Based on Machine Learning-Driven Risk Analysis
https://www.mdpi.com/2076-2615/13/23/3728
How do we reduce unnecessary culling of poultry after HPAI incursion into farms? Here a data-driven method to generate rule tables and risk scores for individual farms, with an accuracy of 84%.
Genetically Related Avian Influenza H7N9 Viruses Exhibit Different Pathogenicity in Mice
https://www.mdpi.com/2076-2615/13/23/3680
Revisiting the surge of human cases of H7N9. Viruses found in poultry had ability to bind mammalian receptors, had PB2-627K marker, and were pathogenic in mice. Monitoring of viruses in poultry is critical. Not particularly novel, I think this was pretty well established.
Influenza virus immune imprinting dictates the clinical outcomes in ferrets challenged with highly pathogenic avian influenza virus H5N1
https://www.frontiersin.org/articles/10.3389/fvets.2023.1286758/abstract
Full version not yet available. “Ferrets were imprinted following H1N1 and H2N3 virus infections were completely protected against lethal H5N1 influenza virus challenge (100% survival) with little to no clinical symptoms” suggesting human influenza infection with H1N1 could be protective for humans (but not H3N2)
Effect of 2020–21 and 2021–22 Highly Pathogenic Avian Influenza H5 Epidemics on Wild Birds, the Netherlands
https://wwwnc.cdc.gov/eid/article/30/1/23-0970_article
HPAI detected in 51 bird species in the Netherlands, alone. In 2020/21 mostly in Anatidae, and in 2021/22 mostly seabirds. Challenge in predicting future trends, so monitoring critical.
Analysis of miRNA expression in the trachea of Ri chicken infected with the highly pathogenic avian influenza H5N1 virus
https://vetsci.org/pdf/10.4142/jvs.23141
miRNA expression patterns of tracheal tissues from H5N1-infected Ri chickens showed differential expression in transforming growth factor-beta, mitogen-activated protein kinase, and Toll-like receptor signaling pathways.
Detection and spread of high pathogenicity avian influenza virus H5N1 in the Antarctic Region
https://www.biorxiv.org/content/10.1101/2023.11.23.568045v1
Analysis of genomes from South Georgia and the Falklands. They are unlinked, so 2 independent incursions into the region.
Pathogenicity in Chickens and Turkeys of a 2021 United States H5N1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b Wild Bird Virus Compared to Two Previous H5N8 Clade 2.3.4.4 Viruses
https://www.mdpi.com/1999-4915/15/11/2273
In comparing 2.3.4.4b H5N1 to previous 2.3.4.4 HPAI viruses, there are key differences in clinical signs, mean death times, and virus transmissibility bw chickens and turkeys
The role of vaccination and environmental factors on outbreaks of high pathogenicity avian influenza H5N1 in Bangladesh
https://www.sciencedirect.com/science/article/pii/S2352771423001751
Vaccination against HPAI in Bangladesh resulted in a ten-fold ⬇️ in outbreak risk. Increase in outbreak rate were low ambient temperatures, literacy rate, chicken density, crop density, and presence of highways.
Outbreak of Highly Pathogenic Avian Influenza Virus H5N1 in Seals in the St. Lawrence Estuary, Quebec, Canada
https://www.biorxiv.org/content/10.1101/2023.11.16.567398v1.abstract
Outbreak of HPAI in in Harbour and Grey Seals in the St. Lawrence estuary. Infection likely due to presence of large numbers of bird carcasses infected with H5N1 at haul-out sites.
Avian influenza viruses in wild birds in Canada following incursions of highly pathogenic H5N1 virus from Eurasia in 2021/2022
https://www.biorxiv.org/content/10.1101/2023.11.23.565566v1.full.pdf
In Canada, 6,246 sick/dead wild birds across 12 taxonomic orders and 80 species tested for HPAI = 27.4% HPAI positive. A further 11,295 asymptomatic harvested/live captured wild birds tested = 5.2% HPAI +ve. Huge and comprehensive effort!
Challenges for Precise Subtyping and Sequencing of a H5N1 Clade 2.3.4.4b Highly Pathogenic Avian Influenza Virus Isolated in Japan in the 2022–2023 Season Using Classical Serological and Molecular Methods
https://www.mdpi.com/1999-4915/15/11/2274
The continuous evolution of HPAI means that diagnostics need to be constantly evaluated for sensitivity against new strains. Comprehensive overview using case study in Japan.
Risk for waterborne transmission and environmental persistence of avian influenza virus in a wildlife/domestic interface in Mexico
https://www.researchsquare.com/article/rs-3606932/v1
Many questions still unanswered around survival/transmissibility of AIV in the environment. Water a key factor. e.g all poultry farms evidence a moderate or high risk of waterborne transmission especially farms close to water bodies
Genotype Diversity, Wild Bird-to-Poultry Transmissions, and Farm-to-Farm Carryover during the Spread of the Highly Pathogenic Avian Influenza H5N1 in the Czech Republic in 2021/2022
https://www.preprints.org/manuscript/202211.0545/v1
Close relationships between H5N1 genomes from poultry and wild birds and secondary transmission in commercial geese in Czech Republic, and six different HPAI genotypes and reassortment with LPAI viruses.
Optimizing environmental viral surveillance: bovine serum albumin increases RT-qPCR sensitivity for high pathogenicity avian influenza H5Nx virus detection from dust samples
https://journals.asm.org/doi/10.1128/spectrum.03055-23
Environmental sampling for HPAI, especially dust a useful sample type: cheap, non-invasive for animals, simpler, and quicker to carry out. BUT, high amounts of organic substances that can inhibit RT-qPCR reactions. Bovine serum albumin may be useful.
Factors influencing highly pathogenic avian influenza preventive behavior among live poultry market vendors
https://www.sciencedirect.com/science/article/pii/S0032579123007496
Preventive behaviour of live bird market vendors is essential in blocking the transmission of HPAI and reducing occupational exposure. Perceived severity and perceived benefits positively influenced the vendors’ ability to adopt preventive behavior
Identification of key residues of B cell epitopes in hemagglutinin of H6 influenza A virus
https://journals.asm.org/doi/10.1128/spectrum.02059-23
Antigenic sites of some HA subtypes (H1, H3, H5, H9), characterized, but H6, one of the most common in birds, is poorly understood. Here, key residues of antigenic epitopes in H6 mapped through escape mutants using a panel of MAbs.
Weathering the Storm of High Pathogenicity Avian Influenza in Waterbirds
https://doi.org/10.1675/063.046.0113
Review of HPAI
A single immunization with H5N1 virus-like particle vaccine protects chickens against divergent H5N1 influenza viruses and vaccine efficacy is determined by adjuvant and dosage
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2287682
H5N1 virus-like particle vaccine based using insect cell-baculovirus expression system induced high levels of HI antibody titers and provided effective protection against homologous virus hallenge comparable to the commercial inactivated vaccine
Emergence of novel reassortant H3N3 avian influenza viruses with increased pathogenicity in chickens in 2023
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2287683
H3 is a pretty common subtype of influenza in wild birds, generally causing no disease in waterfowl. Morbidity issues in several large-scale egg farms in Jiangsu province, due to H3 infections. HA sequences similar to human H3N8 cases.
Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b Infections in Wild Terrestrial Mammals, United States, 2022
https://wwwnc.cdc.gov/eid/article/29/12/23-0464_article
HPAI in 67 wild terrestrial mammals in the USA. Infected mammals showed primarily neurologic signs – Necrotizing meningoencephalitis, interstitial pneumonia, and myocardial necrosis were the most common lesions. Viruses genetically similar to birds.
Neurotropic Highly Pathogenic Avian Influenza A(H5N1) Virus in Red Foxes, Northern Germany
https://wwwnc.cdc.gov/eid/article/29/12/23-0938_article
A 1-year survey in northern Germany, found 5/110 foxes were infected with HPAI, w a cluster from Jan‒March 2023. Encephalitis and strong cerebral virus replication. Pb2 E627K mutations sporadic.
Highly Pathogenic Avian Influenza A(H5N1) from Wild Birds, Poultry, and Mammals, Peru
https://wwwnc.cdc.gov/eid/article/29/12/23-0505_article
HPAI in wild birds, poultry, and a lion in Peru from November 2022–February 2023. Markers associated with transmission adaptation and antiviral drug resistance detected.
Recombinant duck enteritis virus bearing the hemagglutinin genes of H5 and H7 influenza viruses is an ideal multivalent live vaccine in ducks
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2284301
Farmed ducks play a key role in HPAI transmission. Here a recombinant duck enteritis virus H5/H7 vaccine designed for ducks, specifically. Induced long-lasting HI antibodies against H5 & H7 viruses and provided complete protection against challenge
Environmental Surveillance and Detection of Infectious Highly Pathogenic Avian Influenza Virus in Iowa Wetlands
https://pubs.acs.org/doi/full/10.1021/acs.estlett.3c00668
How long does HPAI last in the environment? Virus isolated from wetlands near HPAI outbreaks. One month later, no detection: increased water temperatures, precipitation, biotic and abiotic factors may have played a role.
Spillover of an endemic avian Influenza H6N2 chicken lineage to ostriches and reassortment with clade 2.3.4.4b H5N1 high pathogenicity viruses in chickens
https://link.springer.com/article/10.1007/s11259-023-10258-z
First detection of reassortant viruses: H6N2 chicken-adapted viruses and HPAI H5N1. These reassortant viruses caused an outbreak in ostriches in South Africa
Evolution and biological characterization of H5N1 influenza viruses bearing the clade 2.3.2.1 hemagglutinin gene
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2284294
HPAI 2.3.2.1 viruses in China, 2017-2020. Subclades 2.3.2.1a to 2.3.2.1f detected, with 58 reassortant genotypes. Viruses were antigenically well-matched with the H5-Re12 vaccine strain used in China
Amplification of avian influenza viruses along poultry marketing chains in Bangladesh: a controlled field experiment
https://www.biorxiv.org/content/10.1101/2023.11.10.566573v1.abstract
Live bird markets are a hotspot for HPAI. New study investigates AIV infections during marketing chains, and with testing during transport/trade, intervention group had lower shedding once arrived at LBM.
Pathogen-prey-predator relations of avian raptors during epizootics of highly pathogenic avian influenza virus HPAIV H5N1 (clade 2.3.4.4b) in Germany
https://www.biorxiv.org/content/10.1101/2023.11.19.567176v1
Raptors are important indicators of HPAIV and its genetic diversity, but sadly are victims in this panzootic. Serosurvey found of 5.0-7.9% HPAI antibodies among White tailed sea eagle nestlings. However, breeding success seems stable. Long term effects?
Mutations in HA and PA affect the transmissibility of H7N9 avian influenza virus in chickens
https://www.sciencedirect.com/science/article/pii/S037811352300264X
Detailed interrogation of H7N9 viruses show that mutations in the HA and PA protein reduced the viral transmissibility in chickens, decreasing the threat for poultry.
Molecular characterization and phylogenetic analysis of highly pathogenic H5N1 clade 2.3.4.4b virus in Bosnia and Herzegovina
https://www.frontiersin.org/articles/10.3389/fvets.2023.1255213/full
Detailed interrogation of HPAI in Mute Swan in Bosnia and Herzegovina. Similar to other European sequences. Mutations in HA (N110S and T139P) and NA genes (H155Y) facilitate host specificity shift and resistance to some antiviral drugs.
Mutational antigenic landscape of prevailing H9N2 influenza virus hemagglutinin spectrum
https://www.cell.com/cell-reports/fulltext/S2211-1247(23)01421-3
Sharp increase in human cases of H9N2 in 2021/22. R164Q, I220T mutations increase viral replication in avian and mammalian cells. T150A, I220T mutations enhance viral replication in mice
Key Amino Acid Residues That Determine the Antigenic Properties of Highly Pathogenic H5 Influenza Viruses Bearing the Clade 2.3.4.4 Hemagglutinin Gene
https://www.mdpi.com/1999-4915/15/11/2249
Amino acid changes at position 120, 126, 141, 156, 185, or 189 (H5 numbering) may be important in antigenic changes within 2.3.4.4. Amino acids at 126, 156, and 189 acted as immunodominant epitopes of H5 viruses.
Hooded Vultures Necrosyrtes monachus scavenge on a mass wreckage of large terns in a major HPAI outbreak in The Gambia: a photo report of scraper-feeder type damage to carcasses
https://journals.uct.ac.za/index.php/ABB/article/view/v3_7/v3_7
Hooded Vultures scavenging on tern carcasses following HPAI outbreak in The Gambia. 10,000 dead terns along entire coastline, with vultures focussing on the cervical vertebrae and tempero-mandibular areas of dead terns.
Global Prevalence and Hemagglutinin Evolution of H7N9 Avian Influenza Viruses from 2013 to 2022
https://www.mdpi.com/1999-4915/15/11/2214
Summary of H7N9 sequences since 2013, which includes pre and post vaccination periods in China.
Hemagglutinin affects replication, stability and airborne transmission of the H9N2 subtype avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0042682223002453
The “internal genes” of H9N2 played a key role in zoonotic spillover events of other avian influenzas. This study shows the key role of PA, and importantly HA, for replication, stability, and airborne transmission of H9N2 viruses between poultry.
Genetic and Biological Properties of H10Nx influenza viruses in China
https://www.sciencedirect.com/science/article/pii/S2095311923003702
H10 avian influenzas have a broad host range, infecting not only all kinds of birds, but also mammals and humans. Detailed analysis of H10N3 isolate from poultry in China replicated efficiently in mice lungs and nasal turbinates without prior adaptation
Clade 2.3.4.4 H5 chimeric cold-adapted attenuated influenza vaccines induced cross-reactive protection in mice and ferrets
https://journals.asm.org/doi/epub/10.1128/jvi.01101-23
Human cases of H5N6 continue to tick along in China. Here, a cold-adapted attenuated influenza vaccine induces humoral antibody response, mucosal immune response, and cellular immune response in mice models. Also good protective immunity in ferrets.
Highly Pathogenic Avian Influenza (H5N1) in humans after the emergence of clade 2.3.4.4b in 2020.
https://jglobalbiosecurity.com/articles/10.31646/gbio.218
Summary of human cases.
Molecular detection and characterization of highly pathogenic H5N1 clade 2.3.4.4b avian influenza viruses among hunter-harvested wild birds provides evidence for three independent introductions into Alaska
https://www.sciencedirect.com/science/article/pii/S004268222300257X
Two independant incursions over the Atlantic, now a new study shows 3 independant incursions via Alaska. Lots of HPAI moving around the globe. More evidence that if it arrives here, its unlikely to arrive just once…
Antibodies elicited by Newcastle disease virus-vectored H7N9 avian influenza vaccine are functional in activating the complement system
https://www.sciencedirect.com/science/article/pii/S2095311923003908
NDV vectored avian influenza vaccines result in undetectable H7N9-specific HI, but high IgG antibodies in chickens. Study clarifies role of complement in protection.
Prevalence of Avian Influenza Virus in Synanthropic Birds Associated with an Outbreak of Highly Pathogenic Strain EA/AM H5N1
https://www.biorxiv.org/content/10.1101/2023.11.08.565892v1.full.pdf
Prevalence of HPAI in synanthropic birds (Columbiformes, Galliformes, and Passeriformes), but higher in raptors. Consumption of infected carcasses is a key pathway. Small study of cases in North America.
Molecular diagnosis and identification of avian influenza H5N8 in Pekin ducks
https://www.researchgate.net/profile/Mohamed-Amer-49/publication/375520037_Molecular_diagnosis_and_identification_of_avian_influenza_H5N8_in_Pekin_ducks_Molekulare_Diagnose_und_Identifizierung_der_Geflugelpest_H5N8_bei_Peking-Enten/links/654d4ac7b1398a779d747411/Molecular-diagnosis-and-identification-of-avian-influenza-H5N8-in-Pekin-ducks-Molekulare-Diagnose-und-Identifizierung-der-Gefluegelpest-H5N8-bei-Peking-Enten.pdf
A six-weeks-old flock of Pekin ducks showed sudden mortality without any signs, and birds that survived had depression, repository and/or nervous signs. HPAI detected, and similar to sequences from Egypt and Asia.
Pacific and Atlantic Sea Lion Mortality Caused by Highly Pathogenic Avian Influenza A(H5n1) in South America
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4611782
Incredible overview of sealion outbreaks due to HPAI in South America. More than 24,000 died in Peru, Chile, Argentina, and Uruguay bw Jan- Sept 2023. Route of infection likely eating infected birds.
Mass Mortality of Sea Lions Caused by Highly Pathogenic Avian Influenza A(H5N1) Virus
https://wwwnc.cdc.gov/eid/article/29/12/23-0192_article
Detailed summary of sea lion outbreaks in Peru. 5224 animals died, coinciding with breeding aggregation. Clinical signs of agonal individuals were mainly neurologic (tremors, convulsions, paralysis) and respiratory. Article includes some videos showing disease signs.
Factors influencing highly pathogenic avian influenza preventive behavior among live poultry market vendors
https://www.sciencedirect.com/science/article/pii/S0032579123007496
Perceived severity and perceived benefits positively influenced the bird market vendors ability to adopt preventive behavior while perceived barriers negatively affected self-efficacy. Timely HPAI information really important!
Highly Pathogenic Avian Influenza A(H5N1) Virus-Induced Mass Death of Wild Birds, Caspian Sea, Russia, 2022
https://wwwnc.cdc.gov/eid/article/29/12/23-0330_article#:~:text=In%20May%202022%2C%20we%20observed,b%20virus.
In May 2022, 25,157 Great black-headed gulls, 3,507 Caspian gulls, 5,641 Caspian terns, and 14 Dalmatian pelicans died due to HPAI in the Caspian Sea ?. Nearly all chicks died.
Rapid Detection of H5 Subtype Avian Influenza Virus Using CRISPR Cas13a Based-Lateral Flow Dipstick
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1283210/abstract
full article isn’t available yet.
Serological exposure to influenza A in cats from an area with wild birds positive for avian influenza
https://onlinelibrary.wiley.com/doi/abs/10.1111/zph.13085
Seroprevalence of HPAI in stray cats in Spain is ~2.19%. Not all mammals munching infected birds are dying. Sadly, they left out the Polish cat outbreaks in their overview.
Isolation and Identification of Novel Highly Pathogenic Avian Influenza Virus (H5N8) Subclade 2.3.4.4b from Geese in Northeastern China
https://journals.asm.org/doi/10.1128/aem.01572-22
An old paper I think I missed. In January 2021, a novel HPAI strain A/goose/China/1/2021(H5N8) was detected. This is prior to the start of the panzootic, and it was a 2.3.4.4b virus.
High pathogenicity avian influenza (H5N1) in Northern Gannets (Morus bassanus): Global spread, clinical signs and demographic consequences
https://onlinelibrary.wiley.com/doi/10.1111/ibi.13275
A previously featured preprint now published in Ibis – amazing summary of HPAI in Northern Gannets in 2022. Affected almost all colonies in the north Atlantic, devastating effects on Bass Rocks, and that some birds survive (with changed eye colour)
Identification of Pre-emptive Biosecurity Zone Areas for Highly Pathogenic Avian Influenza Based on Machine Learning-Driven Risk Analysis
https://www.preprints.org/manuscript/202310.1557/v1
Mass culling for HPAI has welfare and food security implications. Here a data driven model to enhance preventative measures and should help to select farms for monitoring and management of HPAI.
Lessons for cross-species viral transmission surveillance from highly pathogenic avian influenza Korean cat shelter outbreaks
https://www.nature.com/articles/s41467-023-42738-w
High number of HPAI H5 virus infections and antibodies in wild carnivores in the Netherlands, 2020–2022
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2270068
Seroprevalence of HPAI is strikingly high in carnivores – serological evidence for infection was 20% ? in the Netherlands from 2020-2022. Virology high too, with 9.9% infection in 2022.
Highly pathogenic avian influenza A(H5N1) virus infection in foxes with PB2-M535I identified as a novel mammalian adaptation, Northern Ireland, July 202
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.42.2300526
Two foxes and two gulls found in Ireland positive for HPAI. Viruses closely related (=common infection source), but mutations at three locations were unique to the fox sequences including PB2 mutations.
Characterization of high pathogenicity avian influenza H5Nx viruses from a wild harbor seal and red foxes in Denmark, 2021 and 2022
https://onlinelibrary.wiley.com/doi/10.1111/irv.13208
Five mammals tested positive for clade 2.3.4.4b H5Nx HPAIVs in Denmark in 2021-22. Virus from the cubs and the adult fox belonged to two different genotypes, and virus from seal similar to virus from German seal sequence. PB2-E627K in seal & 1 fox cub
The episodic resurgence of highly pathogenic avian influenza H5 virus
https://www.nature.com/articles/s41586-023-06631-2
The story of HPAI H5 since the beginning, leading us to how we got here. Multiple waves, genetic changes, and importantly, a big shift in the epicentre of activity.
Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells
https://journals.asm.org/doi/10.1128/jvi.00906-23
Avian influenza virus strains utilize a broader repertoire and can use less prevalent glycoconjugates, for host cell infection vs human influenza A strains. Both infect human lung via N-glycans, O-glycans, and glycolipids.
Analysis of avian influenza A (H3N8) viruses in poultry and their zoonotic potential, China, September 2021 to May 2022
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.41.2200871
H3N8 viruses in Chinese duck farms, poultry markets, 2021-22. Internal genes shared with H9N2 viruses. Viruses have residues that may favour binding to human-type receptors + replication in mammals. Viruses replicate in mice, but are not lethal.
Dissection of key factors correlating with H5N1 avian influenza virus driven inflammatory lung injury of chicken identified by single-cell analysis
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011685
Transcriptome of 16 cell types in lung tissue of chickens infected with HPAIV and H9N2 LPAI. Infiltrating inflammatory macrophages w massive viral replication, pro-inflammatory cytokines and interaction of various cell populations = poor outcomes
Creating resistance to avian influenza infection through genome editing of the ANP32 gene family
https://www.nature.com/articles/s41467-023-41476-3
Gene edited chickens may lead to influenza resistant chickens via host protein ANP32A. After influenza infection challenge, 9/10 edited chickens remain uninfected. But, virus was quick to evolve to instead use ANP32 proteins, chicken ANP32B and ANP32E.
Establishment of two assays based on reverse transcription recombinase-aided amplification technology for rapid detection of H5 subtype avian influenza virus
https://journals.asm.org/doi/10.1128/spectrum.02186-23
New tools for rapid detection of HPAI: real-time fluorescence and reverse transcription recombinase-aided amplification (RF-RT-RAA) and reverse transcription recombinase-aided amplification combined lateral flow dipstick (RT-RAA-LFD). Results in 30min.
Avian influenza overview June–September 2023
https://efsa.onlinelibrary.wiley.com/doi/abs/10.2903/j.efsa.2023.8328
Another update from EFSA: June-Sept 2023. High activity continues, particularly in colony breeding seabirds. Fewer detections in poultry. Be sure to read thoroughly for a comprehensive update!
Antigenic mapping of the hemagglutinin of the H9 subtype influenza A viruses using sera from Japanese quail (Coturnix c. japonica)
https://journals.asm.org/doi/10.1128/jvi.00743-23
Antigenic mapping is a really useful tool to ensure vaccines appropriately match circulating strains. Here, extensive work done on H9 viruses, useing quail sera. Substitutions at 149, 150, and 180 were impactful, with E180A, R131K/E180A critical.
Genetic characteristics of the first human infection with the G4 genotype eurasian avian-like H1N1 swine influenza virus in Shaanxi Province,China
https://rs.yiigle.com/cmaid/1474489
Not avian influenza, but perhaps of interest. Couldn’t seem to manage to access the paper itself.
Predominance of low pathogenic avian influenza virus H9N2 in the respiratory co-infections in broilers in Tunisia: a longitudinal field study, 2018–2020
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-023-01204-7
Predominance of low pathogenic avian influenza virus H9N2, Northern and Western African GI lineage strains in particular, in respiratory co-infections in broilers in Tunisia.
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022
https://www.biorxiv.org/content/10.1101/2023.10.19.563114v1?rss=1&s=03
In November 2022, HPAI caused an outbreak in a zoological park in S France = dead Tibetan black bear, captive and wild birds. Virus recovered from formalin fixed tissues. Bear and gull sequences shared 99.998% and PB2 E627K mutation.
A systematic review of mechanistic models used to study avian influenza virus transmission and control
https://www.biomedcentral.com/epdf/10.1186/s13567-023-01219-0
What can mechanistic models tell us about avian influenza transmission and control? Optimal control strategies varied between subtypes and local conditions, and depended on the overall objective
Influenza from a One Health Perspective: Infection by a Highly Versatile Virus
https://link.springer.com/referenceworkentry/10.1007/978-3-031-27164-9_18
hefty book chapter.
Prevention of zoonotic spillover: From relying on response to reducing the risk at source
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011504
No evidence for highly pathogenic avian influenza virus H5N1 (clade 2.3.4.4b) in the Antarctic region during the austral summer 2022/23
https://www.biorxiv.org/content/10.1101/2023.10.24.563692v1.full.pdf
We pulled together all testing and observational survey data for HPAI in Antarctica during the austral summer 2022/23. No evidence of any HPAI last year – the virus has, however, arrived this year.
Discovery of Influenza A (H7N2) in a Cat After Admission to an Animal Shelter : A Case Report
https://jsmcah.org/index.php/jasv/article/view/61/59
Discovery of Influenza A (H7N2) in a Cat After Admission to an Animal Shelter. Cat was found 1 block from a live bird market (in the USA), however H7 has not been detected in the market since 2006.
Investigation of H9N2 avian influenza immune escape mutant that lacks haemagglutination activity
https://www.biorxiv.org/content/10.1101/2023.10.03.558847v1.abstract
An immune H9N2 escape mutate, with a G149E mutation in the HA, lost hte ability to agglutinate chicken erythrocytes, while still maintaining replication comparable to the wild-type virus in chicken embryos and cells.
Vaccination of poultry against highly pathogenic avian influenza – part 1. Available vaccines and vaccination strategies
https://efsa.onlinelibrary.wiley.com/doi/full/10.2903/j.efsa.2023.8271
Comprehensive review of avian influenza vaccines available, efficacy and strategies in this EFSA report. An incredible resource worth a detailed read.
Determinants for the presence of avian influenza virus in live bird markets in Bangladesh: Towards an easy fix of a looming one health issue
https://www.sciencedirect.com/science/article/pii/S2352771423001635
Avian influenza is extremely common in live bird markets in Bangladesh, with 49% of stalls selling infected poultry. Biosecurity practices, however, heavily influence the circulation of these viruses in markets.
Genetic characterization of a novel H5N6 subtype highly pathogenic avian influenza virus from goose in China
https://www.sciencedirect.com/science/article/abs/pii/S0163445323005339
HPAI H5N6 virus from a goose similar to that reported from a farmed dog in China. Many mutations that could enhance virus replication or increase virulence in mammals were also identified in the goose virus.
Evolutionary history of human infections with highly pathogenic H5 avian influenza a virus: a new front-line global health threat established in South America
https://academic.oup.com/jtm/advance-article/doi/10.1093/jtm/taad130/7295310
More information pertaining to the human case of HPAI in Ecuador. Genetically, virus similar to what was circulating in poultry.
Characterization of highly pathogenic clade 2.3.4.4b H5N1 mink influenza viruses
https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(23)00393-6/fulltext
HPAI from spanish mink outbreak tested for pathogenicity & transmission potential. Viruses highly virulent in mice. High titres in resp organs, brain, elsewhere. In mink, exposed animals infected but did not transmit (contact animals did not seroconvert)
Low Level of Concern Among European Society About Zoonotic Diseases
https://link.springer.com/article/10.1007/s10393-023-01649-4
Recent survey of public opinion in six European countries (n = 2415 participants) suggests a low concern among Europeans about the risk associated with zoonotic emerging diseases.
Exploring the alternative virulence determinants PB2 S155N and PA S49Y/D347G that promote mammalian adaptation of the H9N2 avian influenza virus in mice
https://link.springer.com/article/10.1186/s13567-023-01221-6
Are mutations other than PB2 627K, 701N important in mammalian adaptation? PB2 + PA mutations ⬆️ pol activity, viral transcription and replication in mammalian cells, severe interstitial pneumonia, excessive inflammatory cellular infiltration in mice
Study of the Interface between Wild Bird Populations and Poultry and Their Potential Role in the Spread of Avian Influenza
https://www.mdpi.com/2076-2607/11/10/2601
A census of wild birds around poultry farms (2019, Italy) show that waterfowl arent often near/on poultry farms, but species like magpies, blackbirds, egrets, doves are. More data on bridge hosts required!
Sequence Analysis of the Malaysian Low Pathogenic Avian Influenza Virus Strain H5N2 from Duck
https://www.mdpi.com/2073-4425/14/10/1973
Distinct characteristics of the Malaysian LPAI H5N2, compared to HPAI H5N2 or H5N1 from ducks or chickens
Many potential pathways to future pandemic influenza
https://www.science.org/doi/10.1126/scitranslmed.adj2379
Concern about an H5N1 avian influenza pandemic has caused alarm since 1997, but there are many other possible routes to pandemic influenza
Vaccination and Antiviral Treatment against Avian Influenza H5Nx Viruses: A Harbinger of Virus Control or Evolution
https://www.mdpi.com/2076-393X/11/11/1628
Review of avian influenza in Egypt, including discussion on implementation of prophylactic and therapeutic control strategies, leading to continuous flock outbreaks with remarkable virus evolution scenarios
Improved Resolution of Highly Pathogenic Avian Influenza Virus Haemagglutinin Cleavage Site Using Oxford Nanopore R10 Sequencing Chemistry
https://www.biorxiv.org/content/10.1101/2023.09.30.560331v1
Nanopore R9 had limitations in resolving low-complexity regions e.g. hemagglutinin cleavage site. R10.4.1 = increased data output, higher average quality ,lower minor pop insertion & deletion freq. For the cleavage site, R10.4.1 90% resolved.
Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Circulating Globally in 2022–2023
https://doi.org/10.1093/infdis/jiad418
Phenotypic testing of 2.3.2.1a and 2.3.4.4b HPAI viruses revealed broad susceptibility to NAIs and baloxavir = viruses remain susceptible to key human antivirals. Novel NA mutations caused reduced zanamivir and peramivir inhibition.
Avian Sarcoma/Leukosis Virus (RCAS)-mediated Over-expression of IFITM3 Protects Chicks from Highly Pathogenic Avian Influenza Virus Subtype H5N1
https://www.sciencedirect.com/science/article/pii/S128645792300134X
Over-expression of the interferon-stimulated gene IFITM3 protects chicks from HPAI clade 2.2.1.2. Is the future virus-resistant chickens?
Modeling long-distance airborne transmission of highly pathogenic avian influenza carried by dust particles
https://www.nature.com/articles/s41598-023-42897-2
What is the role of long distance airbourne transmission of HPAI? Overall, via modelling, concentrations of airborne AI, deposited AI, and combined AI transmitted to other farms were lower than the minimal infective dose for poultry. Validation?
Limited Outbreak of Highly Pathogenic Influenza A(H5N1) in Herring Gull Colony, Canada, 2022
https://wwwnc.cdc.gov/eid/article/29/10/23-0536_article
Limited outbreak of HPAI in Herring Gulls in Canada, summer 2022 – less than 10% of the colony affected.
FLIGHT RISKS – Migratory birds efficiently ferry pathogens around the world. As a warming climate reshapes their journeys, infectious disease experts are on guard for new threats to humans
https://www.science.org/content/article/changing-bird-migrations-threaten-bring-new-infectious-diseases-humans
HPAI reports in waders from IWSG2023
https://x.com/ScottoftheMarsh/status/1708072283137298761?s=20
Genetic and antigenic analyses of H5N8 and H5N1 subtypes high pathogenicity avian influenza viruses isolated from wild birds and poultry farms in Japan in the winter of 2021–2022
https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_23-0121/_article/-char/ja/
Three HPAI genome constellations circulating in Japan 2021/22 (H5N8 and H5N1). Multiple H5 HPAI and LPAIVs disseminate to Japan via transboundary winter migration of wild birds
Environmental transmission of influenza A virus in mallards
https://journals.asm.org/doi/10.1128/mbio.00862-23
Detailed studies of LPAI transmission indicate that viral load in water was the strongest predictor of transmission. Also, highly dependant on point of infection course in individual (before or after peak levels) whether it is able to transmit.
Mass Mortality Event in South American Sea Lions (Otaria flavescens) Correlated to Highly Pathogenic Avian Influenza (HPAI) H5N1 Outbreak in Chile.
https://www.tandfonline.com/doi/full/10.1080/01652176.2023.2265173
Overview of sea lion strandings in Chile since 2009, featuring the current HPAI mass mortalities. Strong correlations between widespread mortality of South Americans sea lions and the occurrences of HPAI in wild birds.
Ecological characterization of 175 low-pathogenicity avian influenza viruses isolated from wild birds in Mongolia, 2009–2013 and 2016–2018
https://onlinelibrary.wiley.com/doi/10.1002/vms3.1281
Surveillance in Mongolia [2009–2013, 2016–2018] demonstrates substantial diversity of avian influenzas. Mongolia is situated as a crossroad of multiple migratory flyways
Deadly avian flu reaches Galápagos Islands Concerns rise for boobies, finches, and other endemic species
https://www.science.org/content/article/deadly-avian-flu-reaches-galapagos-islands
Influenza A(H5N1) Virus Infections in 2 Free-Ranging Black Bears (Ursus americanus), Quebec, Canada
https://wwwnc.cdc.gov/eid/article/29/10/23-0548-f1
HPAI in black bear mother and cub in Canada. Virus detected by immunohistochemistry in brain and liver. Both had PB2 D701N mutation. Viruses similar to Newfoundland H5N1 (2021).
Transmission dynamics and pathogenesis differ between pheasants and partridges infected with clade 2.3.4.4b H5N8 and H5N1 high-pathogenicity avian influenza viruses
https://www.biorxiv.org/content/10.1101/2023.09.22.558959v1.abstract
Outbreak of HPAI in pheasant farms, but partridges unaffected. High does of H5N1 required for transmission, and partridges infected with H5N8 failed to shed or transmit. Fewer mortalities when experimentally infected
Emergence of Highly Pathogenic Avian Influenza A (H5N8) Clade 2.3.4.4b Viruses in Grebes in Inner Mongolia and Ningxia, China, 2021
https://www.sciencedirect.com/science/article/pii/S209531192300326X
Outbreak of HPAI in black-necked grebes in 2021, Inner Mongolia, China. Fecal environmental samples from Eurasian spoonbills. HPAI H5N8 detected, and share a common ancestor with sequences in China in 2020
Evolution and Current Status of Influenza A Virus in Chile: A Review
https://www.preprints.org/manuscript/202309.1224/v1
Substantial overview of avian influenza virus in Chile, since ~2002 and featuring HPAI. Would note that the authors currently publishing on HPAI in Chile seem to be absent from this review.
Highly Pathogenic Avian Influenza (HPAI) strongly impacts wild birds in Peru
https://www.sciencedirect.com/science/article/pii/S0006320723003737
In only a few months H5N1 killed >100,000 birds of 24 different species in protected areas of Peru, with huge impacts on boobies, cormorants and pelicans. Virus has severely affected bird populations and the ecosystem services they provide. Peer-reviewed paper of pre-print already shared
Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Wild Birds, Chile
https://wwwnc.cdc.gov/eid/article/29/9/23-0067_article
Detection and genome sequencing of HPAI from Chile show that sequences from Chile and Peru were closely related to a recent ancestor from North America that was detected during October–November 2022. Suggest multiple incursions. Peer-reviewed paper of pre-print already shared
Can Citizen Science Contribute to Avian Influenza Surveillance?
https://www.mdpi.com/2076-0817/12/9/1183
Mortality databases allowing for the public to report sick and dead birds is extremely useful to the HPAI response. Here, results show that HPAI outbreaks officially reported by WOAH overlapped with sudden increases in records of sick/dead birds. I would want highlight the mortality reporting database they are using: Observation.org
High pathogenicity avian influenza (H5N1) in Northern Gannets: Global spread, clinical signs, and demographic consequences
https://onlinelibrary.wiley.com/doi/epdf/10.1111/ibi.13275
Great summary of HPAI outbreaks in Northern Gannets in 2022: Unusually high mortality was recorded at 75% of global total colonies. Adult survival substantially lower than the preceding 10-year average. Description of black irises in survivors. Peer-reviewed paper of pre-print already shared
Looking beyond the H5 avian influenza viruses
https://doi.org/10.1016/j.cell.2023.08.014
Great overview of avian influenza H3, which is being overshadowed by the H5N1 panzootic. Importantly, avian H3’s have caused 3 human cases, and transmits between ferrets (measurement of mammal-to-mammal transmission ability).
Comparative analysis of PB2 residue 627E/K/V in H5 subtypes of avian influenza viruses isolated from birds and mammals
https://www.frontiersin.org/articles/10.3389/fvets.2023.1250952/full
Few PB2 mutations found in LPAI H5, but an increased prevalence of E627K in avian HPAI H5 sequences, and also more mammalian cases. ~40% conversion of E -> K in human sequences of H5
Using surveillance data for early warning modelling of highly pathogenic avian influenza in Europe reveals a seasonal shift in transmission, 2016–2022
https://www.nature.com/articles/s41598-023-42660-7
Analysis of publicly available surveillance data for HPAI integrated into time-series models may help to predict HPAI in different countries . Also shows substantial shift in seasonality in 2021-22
Distribution and risks of the infections of humans and other mammals with H5 subtype highly pathogenic avian influenza viruses in 2020–2023
https://www.sciencedirect.com/science/article/pii/S0163445323005054
A small anaysis of mammalian cases of HPAI reported to WOAH. 285 outbreaks, most of which occurred in Europe/Americas (sampling bias?). Sadly shows limitations of WOAH data as 20,000 dead S. american sealions not highlighted here.
Detection of H5N1 High Pathogenicity Avian Influenza Viruses in Four Raptors and Two Geese in Japan in the Fall of 2022
https://www.mdpi.com/1999-4915/15/9/1865
In 2022, HPAI arrived early in Japan, with outbreaks in geese and raptors. Genetically, viruses were similar to those found in Japan in 2021, but likely reintroduction from Asia/Siberia via early waterfowl migration
Investigation of risk factors for introduction of highly pathogenic avian influenza H5N1 virus onto table egg farms in the United States, 2022: a case–control study
https://www.frontiersin.org/articles/10.3389/fvets.2023.1229008/full
Factors relevant to HPAI outbreaks in egg farms: wild waterfowl presence, wild bird access to feed, existing control zones, moving vegetation less than 4 times per month, off side method of daily mortality disposal. Lots of protective effects as well.
A Fatal A/H5N1 Avian Influenza Virus Infection in a Cat in Poland
https://www.mdpi.com/2076-2607/11/9/2263
A fatal HPAI infection in a cat in Poland: outdoor cat, fed a diet of raw chicken meat. Respiratory distress and neurological signs. Perivascular infiltration of lymphocytes and histiocytes into brain, with neuronal necrosis
Different Outcomes of Chicken Infection with UK-Origin H5N1-2020 and H5N8-2020 High-Pathogenicity Avian Influenza Viruses (Clade 2.3.4.4b)
https://www.mdpi.com/1999-4915/15/9/1909
Direct inoculation of layer chickens with HPAI showed that H5N8-2020 was more infectious than H5N1-2020, death time was longer for H5N8. Tranmission to contact chickens inefficient. More abundant histological lesions and viral antigens for H5N1.
Strong breeding colony fidelity in northern gannets following high pathogenicity avian influenza virus (HPAIV) outbreak
https://www.sciencedirect.com/science/article/pii/S0006320723003701?dgcid=coauthor#f0005
HPAIV killed at least 50 % of northern gannets, with presence antibodies in juveniles. GPS-tracked adults remained faithful to their breeding sites despite outbreak – no prospecting other colonies
Spreading of the High-Pathogenicity Avian Influenza (H5N1) Virus of Clade 2.3.4.4b into Uruguay
https://www.mdpi.com/1999-4915/15/9/1906
Great to see more data from South America -this time from Uruguay! The viruses shared a common ancestor with viruses detected in Chile/Peru and likely introduced from Argentina
HVT-vectored H7 vaccine protects chickens from lethal infection with the highly pathogenic H7N9 Avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0378113523002043
Novel H7 vaccine, using recombinant turkey herpesvirus vector, rHVT-H7HA, that expresses the HA glycoprotein of HPAIV H7N9. Immunization of chickens with the rHVT-H7HA significantly reduces viral load.
Highly pathogenic avian influenza A virus (HPAIV) H5N1 infection in two European grey seals (Halichoerus grypus) with encephalitis
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2257810
HPAI in grey seals from coastal waters of Netherlands and Germany in Dec 2022 / Feb 2023. Brain and lung tested positive = encephalitis in the absence of a systemic infection. PB2-E627K mutation
Spatio-temporal distribution & seasonality of highly pathogenic avian influenza H5N1 & H5N8 outbreaks in India, 2006-2021
https://journals.lww.com/ijmr/abstract/9900/spatio_temporal_distribution___seasonality_of.40.aspx
Analysis of HPAI data from FAO (EMPRES?), shows 284 outbreaks since 2006, with large surge in 2021. Outbreaks occuring post monsoon until pre summer (Oct – March), with peak in January.
Highly pathogenic avian influenza (H5N1) infection in crows through ingestion of infected crow carcasses
https://www.sciencedirect.com/science/article/pii/S0882401023003637
Experimental infection of House Crows with HPAI shows neurological signs. Infected carcasses were eaten by other crows = transmission confirmed
Detection of H5N1 High Pathogenicity Avian Influenza Viruses in Four Raptors and Two Geese in Japan in the Fall of 2022
https://www.mdpi.com/1999-4915/15/9/1865
In 2022, HPAI arrived early in Japan, with outbreaks in geese and raptors. Genetically, viruses were similar to those found in Japan in 2021, but likely reintroduction from Asia/Siberia via early waterfowl migration
Distribution and risks of the infections of humans and other mammals with H5 subtype highly pathogenic avian influenza viruses in 2020–2023
https://www.journalofinfection.com/article/S0163-4453(23)00505-4/fulltext
A small anaysis of mammalian cases of HPAI reported to WOAH. 285 outbreaks, most of which occured in Europe/Americas (sampling bias?). Sadly shows limitations of WOAH data as 20,000 dead S. american sealions not highlighted here
Highly pathogenic avian influenza A (H5N1) in marine mammals and seabirds in Peru
https://www.nature.com/articles/s41467-023-41182-0
Comprehensive overview of HPAI in Peru, including overview of clinical signs. Genetic analysis confirms viruses are Eurasian-N.American reassortants, & Peru-Chile clade descend from single viral introduction ~ October 2022. PB2 D701N in sea lions.
The neuropathogenesis of highly pathogenic avian influenza H5Nx viruses in mammalian species including humans
https://www.sciencedirect.com/science/article/pii/S016622362300190X
Comprehensive review outlining neuropathogenic features in humans and mammals, due to neuroinvasive and neurotropic potential of HPAI, with viruses able to replicate in various CNS cell types.
Refined semi-lethal aerosol H5N1 influenza model in cynomolgus macaques for evaluation of medical countermeasures
https://www.sciencedirect.com/science/article/pii/S2589004223019077
Model for humans.
Baloxavir marboxil use for critical human infection of avian influenza A H5N6 virus
https://www.medrxiv.org/content/10.1101/2023.09.03.23294799v1
When delayed oseltamivir showed poor effects on high respiratory viral load, baloxavir was prescribed and viral load had a rapid reduction
Asymptomatic infection with clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) in carnivore pets, Italy, April 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.35.2300441
HPAI in 1 domestic dog and 5 cats living on a rural backyard poultry farm. Poultry infected with BB genotype that was characterised by the presence of a PB2 mutation related to mammalian adaptation
Virulence and transmission characteristics of clade 2.3.4.4b H5N6 subtype avian influenza viruses possessing different internal gene constellations
https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2250065
Genome constellations have differential outcomes of HPAI. 2.3.4.4b H5N6 in China, with two genome constellations: constellation w H9 PB2 & PB1 genes on H5 backbone better infect mice, mammalian cells but worse in avian cells, bird transmission.
Highly pathogenic avian influenza affects vultures’ movements and breeding output
https://doi.org/10.1016/j.cub.2023.07.061
Satellite tracked Griffon Vultures in Europe display inferred sickness behaviour via immobility during HPAI infection. Most chicks died but most adults recovered. Great to see integration of movement data to HPAI studies.
Recurring Trans-Atlantic Incursion of Clade 2.3.4.4b H5N1 Viruses by Long Distance Migratory Birds from Northern Europe to Canada in 2022/2023
https://doi.org/10.3390/v15091836
Evidence for yet another viral incursion into North America, likely via the Atlantic between Dec2022-Jan2023. Brings us to 3 incursion events into North America
Epidemiological and clinical characteristics of human infections with avian influenza A (H7N9) and A (H5N6) viruses in Guangdong province, 2013-2018
https://rs.yiigle.com/cmaid/1175653
Summary of epidemiological and clinical characteristics of human infections with avian influenza H7N9 (n=259, CFR 38%)) and H5N6 (n=8, CFR 62%) viruses in Guangdong province, 2013-2018. (In Chinese, unsure how to access)
Surveillance of environmental avian influenza virus in Fujian province, 2017-2021
https://rs.yiigle.com/cmaid/1447498
4214 samples collected from Fujian province (2017-2021). 2.5% H5, 1.16% H7, 23.16 H9. AIV prevalence highest n urban and rural live poultry markets – cage srufaces, cleaning poultry sewage and chopping boards (In Chinese, unsure how to access)
Epidemiological investigation of the first confirmed human case of avian influenza A(H5N6) virus infection in Beijing
https://rs.yiigle.com/cmaid/1257603
Human infection of H5N6 – patient cooked frozen chicken from a market. Sample from patient bronchoalveolar lavage fluid and frozen poultry samples were highly similar. (In Chinese, unsure how to access)
Interpretation of molecular detection of avian influenza A virus in respiratory specimens collected from live bird market workers in Dhaka, Bangladesh: Infection or contamination?
https://www.sciencedirect.com/science/article/pii/S1201971223007063
In 1,273 influenza-like illness cases in Banglaesh, 34 (2.6%) had H5, 56 (4%) had H9 by qPCR. Of 192 asymptomatic workers, H5 was detected in 8 (4%). Of 28 ILI cases with H5 or H9 detected, no seroconversion. Infection or environmental contamination?
Advocating a watch-and-prepare approach with avian influenza
https://www.nature.com/articles/s41564-023-01457-0
short note, with a strong focus on human infections and barriers to human infections. Key messages: (1) “H5 HPAIV remains unlikely to acquire the ability to infect and stably circulate among the human population” (2) “The impact of the current outbreak on livestock industries and wild animal populations is immense and demands intervention”
Will climate change amplify epidemics and give rise to pandemics?
https://www.science.org/doi/full/10.1126/science.adk4500
Key amino acid position 272 in neuraminidase determines the replication and virulence of H5N6 avian influenza virus in mammals
https://doi.org/10.1016/j.isci.2022.105693
Most human HPAI cases are due to H5N6. Since 2015, an increase in the NA-D272N mutation in wild birds, which is associated with increased replication and virulence in mice and induced higher levels of inflammatory cytokines in human cells.
Severe pigeon paramyxovirus 1 infection in a human case with probable post-COVID-19 condition
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2251600
Not influenza, but probably of importance. Recent case of pigeon paramyxovirus (a genotype of avian paramyxovirus 1) in humans in China. The infected person had long COVID – so likely immunocompromised.
Chicken miR-26a-5p modulates MDA5 during highly pathogenic avian influenza virus infection
https://www.sciencedirect.com/science/article/pii/S0145305X23010741
Downregulation in gga-miR-26a [chicken microRNA] in lung of chickens during HPAI infection. Findings suggest that this microRNA serves as an important regulator in the MDA5 signaling pathway and therefore in antiviral response
Immunogenicity and Cross-Protective Efficacy Induced by an Inactivated Recombinant Avian Influenza A/H5N1 (Clade 2.3.4.4b) Vaccine against Co-Circulating Influenza A/H5Nx Viruses
https://www.mdpi.com/2076-393X/11/9/1397
Multiple strains of HPAI circulating in Egypt and vaccine mismatch reported. Here, 3 reverse-genetics H5Nx vaccines generated. Superior immunogenicity and cross-protective efficacy of the rgH5N1_2.3.4.4 in comparison to rgH5N8_2.3.4.4 and rgH5N1_2.2.1.2
Emergence and Persistent Circulation of Highly Pathogenic Avian Influenza Virus A(H5N8) in Kosovo, May 2021–May 2022
https://www.preprints.org/manuscript/202308.1579/v1
Three outbreaks of HPAI in Kosovo: May–June 2021, September–November 2021, and January–May 2022 with 32 backyard+10 commercial poutry houses affected = 179,198 poultry. H5N8 clade 2.3.4.4.b viruses implicated.
Praemonitus praemunitus: can we forecast and prepare for future viral disease outbreaks?
https://doi.org/10.1093/femsre/fuad048
“future viral epidemics are unavoidable, but that their societal impacts can be minimized by strategic investment into basic virology research, epidemiological studies of neglected viral diseases, and antiviral drug discovery”. Would add surveillance!
Emergence of a new genotype of clade 2.3.4.4b H5N1 highly pathogenic avian influenza A viruses in Bangladesh
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2252510
Novel clade 2.3.4.4b H5N1 virus from ducks in free-range farms in Bangladesh. Similar to viruses first detected in October 2020 in The Netherlands but with a different PB2
Pilot of asymptomatic swabbing of humans following exposures to confirmed avian influenza A(H5) in avian species in England, 2021/2022
https://onlinelibrary.wiley.com/doi/full/10.1111/irv.13187
1617 human exposures to HPAI in England. Asymptomatic swabbing of humans revealed 1 detection. Human surveillance (at human: poultry interface) critical for ongoing HPAI monitoring!
Innate immune control of influenza virus interspecies adaptation
https://www.biorxiv.org/content/10.1101/2023.08.23.554491v1.full.pdf
IFITM3 [host antiviral factor] deficiency in humans due to SNPs, with 20% of some human pops homozyg for deficient gene. IFITM3 facilitates zoonotic influenza infects & adaptation. IFITM3 deficiencies = vulnerability for emergence of new pandemic viruses
Exploring the responses of smallscale poultry keepers to avian influenza regulations and guidance in the United Kingdom, with recommendations for improved biosecurity messaging
https://www.cell.com/heliyon/pdf/S2405-8440(23)06419-8.pdf
Regulations only work if understood and accepted. Survey of small scale poultry keepers in UK in 2021/22. Need for guidance tailored to smallscale poultry keepers including clear action points w simple, practical, affordable and sustainable suggestions
Functional traits explain waterbirds’ host status, subtype richness, and community-level infection risk for avian influenza
https://onlinelibrary.wiley.com/doi/epdf/10.1111/ele.14294?saml_referrer
The host range of HPAI has expanded dramatically, although many species likely just dead-end spill over hosts. New study shows the key role of functional traits in explaining HPAI infection risk: functional diversity can reduce infection risk
BAITING AND BANDING: EXPERT OPINION ON HOW BAIT TRAPPING MAY INFLUENCE THE OCCURRENCE OF HIGHLY PATHOGENIC AVIAN INFLUENZA (HPAI) AMONG DABBLING DUCKS
https://doi.org/10.7589/JWD-D-22-00163
no access ☹
Infection and tissue distribution of Highly Pathogenic Avian Influenza A type H5N1 (clade 2.3.4.4b) in Red Fox kits (Vulpes vulpes)
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2249554
Fox kits infected with HPAI have severe neurological signs and brain (and lung) tissue lesions. Labelled showed infection was clustered and overlapped the brain lesions, neurons, and grey matter. V292I mutation in PB2.
Human infection with avian-origin H5N6 influenza a virus after exposure to slaughtered poultry
https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2048971
Human case of HPAI H5N6 virus in China after exposure to freshly slaughtered chicken (not live birds). Q226 & G228 mutation in HA – affect binding affinity of α-2,6-linked sialic acid receptor. No PB2 mutation. Viruses related to those in poultry market.
Clinical features of the first critical case of acute encephalitis caused by the avian influenza A (H5N6) virus
https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2122584
Acute encephalitis with mild pneumonia in a child in China caused by the H5N6 virus. HPAI found in patient’s serum, CSF, and tracheal aspirate specimens. Virus similar virus in human infection and in ducks in China.
Emergence of a new designated clade 16 with significant antigenic drift in hemagglutinin gene of H9N2 subtype avian influenza virus in eastern China
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2249558
Major switches to antigenic properties in novel H9N2 lineage, potentially having ramifications for H9 vaccination in China. There is a history of vaccine effectiveness against H9 being persistently challenged with the evolution of new lineages.
Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus
https://www.tandfonline.com/doi/abs/10.1080/03079457.2023.2239191
Rather than focus on only the HA, the authors argue that the NA should also be considered in vaccine design for H9N2. Slower antigenic drift in NA compared to HA, and identified epitopes are highly conserved.
Evolution of prevalent H9N2 subtype of avian influenza virus during 2019 to 2022 for the development of a control strategy in China
https://www.sciencedirect.com/science/article/pii/S0032579123004765
Current circling H9N2 viruses in China have diversified into h9.4.2.5 subclade, which is genetically distant from commonly used commercial vaccine strains. Development of novel recombinant vaccine with new strain outperformed existing vaccine in trials.
Avian influenza A(H5N1) and the continuing outbreak
https://ncceh.ca/resources/evidence-briefs/avian-influenza-ah5n1-and-continuing-outbreak
From the National Collaborating Centre for Environmental Health (Canada)
Assessment of contaminants, health and survival of migratory shorebirds in natural versus artificial wetlands – The potential of wastewater treatment plants as alternative habitats
https://www.sciencedirect.com/science/article/pii/S0048969723049343
New paper by Marcel’s PhD student Toby, investigating pollutants and links to waste water treatment sites and disease burden in wild birds.
Scottish wild bird highly pathogenic avian influenza response plan
https://www.gov.scot/publications/scottish-wild-bird-highly-pathogenic-avian-influenza-response-plan/
Compliments sections in the UK response plan and also provides sections on “HPAI in wild birds – research and monitoring” and Advice for Rehab organisations”
The plight and role of wild birds in the current bird flu panzootic
https://www.nature.com/articles/s41559-023-02182-x
We outline the plight of wild birds in the HPAI panzootic, and our concerns around the severe discrepancy in reporting of mortality data.
‘One Health’ Genomic Surveillance of Avian and Human Influenza A Viruses Through Environmental Wastewater Monitoring
https://www.medrxiv.org/content/10.1101/2023.08.08.23293833v1
Wastewater has been useful for virus detection from humans. Water treatment plants are extremely important environments for birds, and so an intersting environmental sample type for detection of HPAI
Research Note: A Recombinant Duck-derived H6N2 Subtype Avian Influenza Virus can Replicate and Shed in Young Chickens and Cause Disease
https://www.sciencedirect.com/science/article/pii/S003257912300531X
H6 avian influenza causing high morbidity in poultry in China. In challenge, caused morbidity in chickens but not ducks. PB1 most similar to that of an H5N6 virus.
The first known human death after infection with the avian influenza (A/H3N8) virus: Guangdong Province, China, March 2023
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciad462/7239858
Case report of human cause of H3N8 in an immunocompromised patient causing mortality in China. No mammalian adaptations. Testing of local market environment had evidence of H3 in cutting tools, feeding trough and sinks
Alteration of the chicken upper respiratory microbiota, following H9N2 avian influenza virus infection
https://www.biorxiv.org/content/10.1101/2023.08.08.549695v1.abstract
AIV infection has ramifications for the microbiomes of birds. H9 lowers alpha diversity in the upper resp tract in chickens, with enrichment of Lactobacillis. Also, microbial community didn’t return to normal after infection.
Comparative analysis of PB2 residue 627E/K/V in H5 subtypes of avian influenza viruses isolated in birds and mammals
https://www.frontiersin.org/articles/10.3389/fvets.2023.1250952/abstract
ahead of print, no details yet.
Diagnostic Detection of H7N3 Low Pathogenicity Avian Influenza in a Commercial Game Bird Flock
https://doi.org/10.1637/aviandiseases-D-22-00055
no access ?
The emergence of new antigen branches of H9N2 avian influenza virus in China due to antigenic drift on hemagglutinin through antibody escape at immunodominant sites
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2246582
Detailed mapping of resides important for antigenic drift (away from vaccine strains) in china. Nice to see structural mapping in addition to sequence analysis.
Enhanced Thermostability and Provides Effective Immune Protection against Lethal H7N9 Virus Challenge in Chickens
https://www.mdpi.com/2076-393X/11/8/1318
Adding a T169A mutation to the HA of H7 increased therostability, and vaccinating chickens with this strain also increases cross reactivity and cytokine secretion. In challenge trials, 90% of chickens had no viral shedding.
Pathogenicity of H5N8 avian influenza virus in chickens and in duck breeds and the role of MX1 and IFN-α in infection outcome and transmission to contact birds
https://doi.org/10.1016/j.cimid.2023.102039
A duck is not a duck is not a duck. Differential disease outcomes when comparing Muscovy, Pekin and Mallard ducks infected with HPAI 2.3.4.4b H5N8. No Mallards died, and had highest Mx and INF expression and highest shedding.
Host gene expression is associated with viral shedding magnitude in blue-winged teals (Spatula discors) infected with low-path avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0147957122001667?via%3Dihub
Old paper that I had missed. Nice to see infection experience of species other than mallards for once. And they used RNASeq as well. Outcomes are prtty much as expected. Lots happening in the illum. Lots of the innate immne genes upregulated.
Novel Reassortant Avian Influenza A(H5N6) Virus, China, 2021
https://wwwnc.cdc.gov/eid/article/28/8/21-2241_article
Description of clade 2.3.4.4b HPAI H5N6 in China. These viruses havestrong immune-escape capacity and complex genetic reassortment, suggesting further transmission risk
H5N1 highly pathogenic avian influenza clade 2.3.4.4b in wild and domestic birds: Introductions into the United States and reassortments, December 2021–April 2022
https://www.sciencedirect.com/science/article/pii/S0042682223001733?via%3Dihub
Huge study of HPAI genetics in the USA. THREE!! distinct HPAI viruses (3 introductions?), local reassortment with LP viruses, and complex patterns of spatial diffusion
Prediction of highly pathogenic avian influenza vaccine efficacy in chickens by comparison of in vitro and in vivo data: A meta-analysis and systematic review
https://www.sciencedirect.com/science/article/pii/S0264410X23009222
Huge evaluation of vaccines for HPAI. HI titers when using the challenge virus as antigen = good predictor of vaccine efficacy. HA1 relatedness has limitations when predicting efficacy for rHVT-vector and RP vaccines. Lots of good stuff
Influenza A Virus in Pigs in Senegal and Risk Assessment of Avian Influenza Virus (AIV) Emergence and Transmission to Human
https://www.mdpi.com/2076-2607/11/8/1961
In a survey of pigs in Senegal, avian influenza H5, H7, H9 antibodies found in pigs (although more typical H1 and H3 swine and human lineages found via virology).
Outbreak of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b virus in cats, Poland, June to July 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.31.2300366
Detailed report on 25 of the Polish cats, interrogation of 19 full HPAI genomes. No clustering based on geography. To date, are the only sequences with dual PB2 526R/627K mutations for mammalian infection
Emergence and potential transmission route of avian influenza A (H5N1) virus in domestic cats in Poland, June 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.31.2300390
A nice summary of the cluster of HPAI cases in Polish cats (at least 89!), including indoor cats. Genomes from all cats identical, including 2 PB2 mutations (PB2-E627K and PB2-K526R). HPAI detected in frozen chicken meat – infected via food.
Highly pathogenic avian influenza A(H5N1) virus infection on multiple fur farms in the South and Central Ostrobothnia regions of Finland, July 2023
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.31.2300400
From 14-27 July, 20 fur farms affected by HPAI, Finland. (There are 500 in Finland). Animal in cages, with a roof, but no walls. Genome sequence similar to gulls and birds have access to the houses. PB2 mutations detected. Animals will be culled.
Characterization of the haemagglutinin properties of the H5N1 avian influenza virus that caused human infections in Cambodia
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2244091
Detailed work on the HPAI viruses [2.3.2.1c] that infected people in Cambodia shows similar profiles to old strains with no evidence of improved binding to humans but improved acid and thermal stability. Virus poses limited zoonotic risk.
Waterfowl show spatiotemporal trends in influenza A H5 and H7 infections but limited taxonomic variation
https://esajournals.onlinelibrary.wiley.com/doi/10.1002/eap.2906
Detailed interrogation of the drivers affecting prevalence of (low path) H5 and H7 in the US. H5 viruses in late autumn and H7 viruses in spring. Large differences in trends over all influenzas, but few differences for these subtypes.
Evolution and Reassortment of H6 Subtype Avian Influenza Viruses
https://www.mdpi.com/1999-4915/15/7/1547
Report of H6 viruses from poultry and wild birds in China, collected since 2001. Viruses common in ducks – reflecting results from elsewhere.
Continued Circulation of Highly Pathogenic H5 Influenza Viruses in Vietnamese Live Bird Markets in 2018–2021
https://www.mdpi.com/1999-4915/15/7/1596
Between 2018-2021 (prior to the start of the panzootic), 2.3.4.4g and 2.3.4.4h H5N6 dominated in live bird markets of Vietnam. In 2016-17, 2.3.2.1c also present.
Evolution of Prevalent H9N2 Subtype of Avian Influenza Virus during 2019-2022 for the Development of a Control Strategy in China
https://www.sciencedirect.com/science/article/pii/S0032579123004765
H9N2 viruses in China diversified into h9.4.2.5, which was genetically distant from commonly used commercial vaccine strains. A new vaccine generated showed improved outcomes (due to a better match).
The Feather Epithelium Contributes to the Dissemination and Ecology of clade 2.3.4.4b H5 High Pathogenicity Avian Influenza Virus in Ducks
https://www.biorxiv.org/content/10.1101/2023.07.26.550633v1.abstract
We know that you can detect AI on bird feathers, and this may play a role in transmission. In HPAI outbreaks, viruses exhibited persistent and marked feather epitheliotropism in infected commercial ducks = source of environmental infectious material.
Emergence of a novel reassortant H5N6 subtype highly pathogenic avian influenza virus in farmed dogs in China
https://www.sciencedirect.com/science/article/pii/S0163445323003869
2.3.4.4.b HPAI H5N6 found in the spleen of a farmed dog in China.
Characterization of avian influenza A (H4N2) viruses isolated from wild birds in Shanghai during 2019-2021
https://www.sciencedirect.com/science/article/pii/S0032579123004674
H4 LPAI viruses are some of the most common in duck surveillance systems. Additional sequences from China, although H4N2 rather than H4N6. Most internal genes were LPAI, but the PB1 of one virus most similar to a HPAI H5N8 virus
Susceptibility of common dabbling and diving duck species to clade 2.3.2.1 H5N1 high pathogenicity avian influenza virus: an experimental infection study
https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_23-0122/_pdf/-char/en
Observations from previous outbreaks have suggested that diving ducks are hard hit when infected with HPAI. Experimental infections (with 2.3.2.1) confirm this, with higher titres and mortality in Tufted ducks compared to wigeons mallards and pintails.
Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Wild Birds, Chile
https://pubmed.ncbi.nlm.nih.gov/37487166/
Ahead of print, so can’t get to the pdf to read it yet.
Mortality in Wild Turkey (Meleagris gallopavo) Associated with Natural Infection with H5N1 Highly Pathogenic Avian Influenza Virus (HPAIV) Subclade 2.3.4.4
https://meridian.allenpress.com/jwd/article-abstract/doi/10.7589/JWD-D-22-00161/494554/Mortality-in-Wild-Turkey-Meleagris-gallopavo?redirectedFrom=PDF
No access ☹
Epidemiology and phylodynamics of multiple clades of H5N1 circulating in domestic duck farms in different production systems in Bangladesh
https://www.frontiersin.org/articles/10.3389/fpubh.2023.1168613/full
More data from Bangladesh, wherein there is co-circulating lineages of HPAI H5, as well as H9 and others. ducks from nomadic farms, juvenile, and sick ducks had a higher risk of AIV
Genetic and Biological Characterization of H3N2 Avian Influenza Viruses Isolated from Poultry Farms in China between 2019 and 2021
https://www.hindawi.com/journals/tbed/2023/8834913/
Large diversity of AIVs, but also isolated H3N2 in poultry in China. Since some of the H3N2 viruses replicated without preadaptation and caused body weight loss in mice (due to a PB2 E627K) Note. They also detected H14!!
Early-life prophylactic antibiotic treatment disturbs the stability of the gut microbiota and increases susceptibility to H9N2 AIV in chicks
https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-023-01609-8
Using antibiotic to promote poultry growth makes chicks more susceptible to H9N2! More studies linking the gut microbiome with influenza virus susceptibility!
Antiviral susceptibility of clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) viruses isolated from birds and mammals in the United States, 2022
https://doi.org/10.1016/j.antiviral.2023.105679
Sporadic drug resistance in HPAI viruses in the USA: three adamantane-resistant (M2-V27A), four oseltamivir-resistant (NA-H275Y), and one baloxavir-resistant (PA-I38T)
The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals
https://www.science.org/doi/10.1126/sciadv.adg5175
HPAI is still an avian virus, and often requires adaptive mutations to allow for infection in mammals. In H9N2, they found that the NS2 protein can also help overcome mammalian restriction to the avian polymerase. Good explanation twitter thread here: https://twitter.com/ejustin46/status/1684198237547245569?s=20
Phylogenetic and Pathogenicity Comparison of Three H5N6 Avian Influenza Viruses in Chickens, Ducks and Mice
https://europepmc.org/article/ppr/ppr694871
HPAI H5N6 causes numerous human cases in China. Comparison of 3 strains from birds shows 2.3.4.4h. Mutations with cross-species transmission and enhanced pathogenicity found. Differences in infection outcomes in chickens, ducks and mice
Connect to Protect: Dynamics and Genetic Connections of Highly Pathogenic Avian Influenza Outbreaks in Poultry from 2016 to 2021 in Germany
https://www.mdpi.com/1999-4915/14/9/1849
Old one, but shows how poultry clusters are connected. Wild bird-mediated entries into backyard holdings, several clusters of poultry holdings, local virus circulation, farm-to-farm transmission and spill-over into the wild bird populations
Loss of amino acids 67-76 in the neuraminidase protein under antibody selection pressure alters the tropism, transmissibility and innate immune response of H9N2 avian influenza virus in chickens
https://www.sciencedirect.com/science/article/pii/S0378113523001840
Minor changes to the neuraminidase alters the tropism and host immune response against H9N2 in poultry. These changes have been identified in nature, and antibody selection plays role in evolution of H9N2
Highly pathogenic avian influenza A(H5N1) virus in a common bottlenose dolphin (Tursiops truncatus) in Florida
https://www.researchsquare.com/article/rs-3065313/v1
Case report of HPAI in a bottle nosed dolphin in Florida. Neuronal necrosis, inflammation of the brain, highest viral load in brain. S246N neuraminidase substitution = reduced inhibition by the neuraminidase inhibitor oseltamivir
Quail Rearing Practices and Potential for Avian Influenza Virus Transmission, Bangladesh
https://link.springer.com/article/10.1007/s10393-023-01643-w
Survey of people who have Quail, all respondents (67) reported keeping quail with other birds in cages, feeding quail, cleaning feeding pots, removing quail faeces, slaughtering sick quail, and discarding dead quail. Children played with quail and assisted in slaughtering of quail. Most respondents (94%) reported rinsing hands with water only after slaughtering and disposing of wastes and dead quail. No personal protective equipment was used during any activities.
Impact of palmiped farm density on the resilience of the poultry sector to highly pathogenic avian influenza H5N8 in France
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-023-01183-9
Palmiped = web footed
Decreasing density of ducks/geese in the areas of highest density substantially decreases Ro in HPAI outbreaks. Much higher benefit compared to reducing chicken densities. Great study from France.
Investigation of risk factors for introduction of highly pathogenic avian influenza H5N1 virus onto table egg farms in the United States, 2022: a case-control study
https://www.frontiersin.org/articles/10.3389/fvets.2023.1229008/abstract
Final version not yet available.
Safety and Immunogenicity of a Delayed Heterologous Avian Influenza A(H7N9) Vaccine Boost Following Different Priming Regimens: A Randomized Clinical Trial
https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiad276/7226356
In a randomised controlled study, humans that received an H7N9 vaccine with an adjuvant, and a prime and boost regime had the best serological response.
Art of the Kill: Designing and Testing Viral Inactivation Procedures for Highly Pathogenic Negative Sense RNA Viruses
https://www.mdpi.com/2076-0817/12/7/952
Chemical inactivation works well. Heat does so, when done properly. Some SOPs provided also.
Avian Influenza: A Potential Threat to Human Health
https://link.springer.com/chapter/10.1007/978-981-99-2820-0_3
book chapter
Mink farming poses risks for future viral pandemics
https://www.pnas.org/doi/abs/10.1073/pnas.2303408120
Great summary from @PeacockFlu and Wendy Barclay on risk of minks (and other fur farmed species) play in viral pandemics, including HPAI. And see this great Twitter thread by Tom Peacock: https://twitter.com/PeacockFlu/status/1682696057744916480?s=20
Evaluation of the Effect of Pb Pollution on Avian Influenza Virus-Specific Antibody Production in Black-Headed Gulls (Chroicocephalus ridibundus)
https://www.mdpi.com/2076-2615/13/14/2338
Increased blood lead (Pb) levels in Black-headed gulls associated with a significant decrease in avian influenza antibody titre. Wonder if its a factor in the current black-headed gull HPAI outbreaks?
Annual trading patterns and risk factors of avian influenza A/H5 and A/H9 virus circulation in turkey birds (Meleagris gallopavo) at live bird markets in Dhaka city, Bangladesh
https://www.frontiersin.org/articles/10.3389/fvets.2023.1148615/full
H5 and H9 found in turkeys in Bangladesh. Turkeys traded ~180km. AIV increases in retail vendor business and the bird’s health status is sick or dead. Seasonality also at play.
Subtype specific virus enrichement with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus
https://www.sciencedirect.com/science/article/pii/S0166093423000988
Sequencing samples co-infected with avian influenza is challenging (which segment belongs to which virus?). Here, an immunomagnetic separation method is proposed, prior to NGS sequencing.
Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2184177
H13 is host restricted to gulls with few detections in other avian taxa. Infection experiments show they can replicate in chicken (but not quail or turkey) and mammalian cells lines. Serology screening showed 4.6-10% antibody positive in farm animals!
SCIENTIFIC TASK FORCE ON AVIAN INFLUENZA AND WILD BIRDS STATEMENT – JULY 2023
https://www.cms.int/sites/default/files/publication/CMS-FAO-TF_avian_influenza_statement_July2023.pdf
A Dutch highly pathogenic H5N6 avian influenza virus showed remarkable tropism for extra-respiratory organs and caused severe disease but was not transmissible via air in the ferret model
https://journals.asm.org/doi/10.1128/msphere.00200-23
Also, the mammalian mutation D701N was positively selected for.
Highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.4b in domestic ducks, Indonesia, 2022
https://www.biorxiv.org/content/10.1101/2023.07.10.548369v1.full.pdf
HPAI 2.3.4.4b was detected in Indonesia in April 2022 for the first time (other lineages do circulate in Indonesia). In a clade of viruses in Asia, including China and Japan in 2021/2022. Results important for understanding risk to Australia
Comparative Investigation of Coincident Single Nucleotide Polymorphisms Underlying Avian Influenza Viruses in Chickens and Ducks
https://www.mdpi.com/2079-7737/12/7/969
co SNPS on AIV-related differentially expressed genes and effects that occur in both the duck and chicken genomes help reveal shared immune pathways in these species
Relationship between some H5 commercial vaccines and the highly pathogenic H5N8 avian influenza virus that is recently circulating in Egypt
https://rjab.journals.ekb.eg/article_306838_0.html
I cant figure out how to access the article yet as its ahead of print, so I will just flag this as something that is likely to be interesting to read once its out.
Development and Validation of Competitive ELISA for Detection of H5 Hemagglutinin Antibodies
https://www.mdpi.com/2674-1164/2/3/26
Development of cELISA that detects H5 antibodies (including HPAI and LPAI H5) in wild bird and poultry.
Cross-species transmission and PB2 mammalian adaptations of highly pathogenic avian influenza A/H5N1 viruses in Chile
https://www.biorxiv.org/content/10.1101/2023.06.30.547205v1.full.pdf
23 avian and 3 mammal orders were tested for HPAI in Chile, with sequences now from 77 birds and 8 mammals. Sequences cluster monophyletically with viruses from Peru = single introduction from NAmerica. 3 scenarios for spread of mammalian mutation D701N
The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011489
H5 subtype AIV PA protein strongly suppresses host antiviral defense in mammals, specifically 2 mutations inhibit the IFN-mediated immune response.
Animal Markets and Zoonotic Disease in the United States
https://animal.law.harvard.edu/wp-content/uploads/Animal-Markets-and-Zoonotic-Disease-in-the-United-States.pdf
A report commissioned to understand the zoonotic risk from mass produced animals.
Summary of Influenza Risk Assessment Tool (IRAT) Results (4 July 2023)
https://www.cdc.gov/flu/pandemic-resources/monitoring/irat-virus-summaries.htm
CDC has updated the risk assessment of HPAI, taking into consideration the viruses from the mink farm outbreak. The newer virus from the minks scored higher than the earlier duck virus on 6 of the 10 risk elements, which included antiviral treatment options, disease severity and pathogenesis, genomic analysis, human infections, infections in animals, and transmission in animal models.
Genetic characteristics of waterfowl-origin H5N6 highly pathogenic avian influenza viruses and their pathogenesis in ducks and chickens
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1211355/full
Since 2013 2.3.4.4h HPAI found in ducks in China. Experimental infections show ducks survive, and some have mild clinical infections but chickens have severe disease
Biological features of human influenza A(H3N8) viruses in China
https://onlinelibrary.wiley.com/doi/10.1002/jmv.28912
More work on those human H3N8 viruses. Human H3N8 exhibited dual receptor binding profiles (avian H3N8 viruses bound to only avian type). All H3N8 viruses were sensitive to the antiviral drug oseltamivir
Investigating the Genetic Diversity of H5 Avian Influenza Viruses in the United Kingdom from 2020–2022
https://journals.asm.org/doi/epub/10.1128/spectrum.04776-22
Virus genomic data from the UK eloquently demonstrate the shift to 2.3.4.4b H5N1 at the end of 2021 from H5Nx the previous year. Large diveresity of reassortants.
Continuous surveillance of potentially zoonotic avian pathogens detects contemporaneous occurrence of highly pathogenic avian influenza viruses (HPAIV H5) and flaviviruses (USUV, WNV) in several wild and captive birds
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2231561
Literature review to reveal overlap in host range for orthomyxos (HPAI) and flavis (West Nile, Usutu) in Europe to better understand role of wild birds in maintenance of zoonotic viruses.
Estimated mortality of the highly pathogenic avian influenza pandemic on northern gannets (Morus bassanus) in southwest Ireland
https://royalsocietypublishing.org/doi/full/10.1098/rsbl.2023.0090
Given limits in collection of wild bird mortality data during HPAI, authors attempted to estimate mortality of Northern Gannets in SW Ireland: the estimated minimum local population mortality was 3126 birds.
Vogelgriep panzoötie leidt tot massastrandingen van Jan-van-genten Morus bassanus in Nederland, april-oktober 2022 [In Dutch]
https://www.researchgate.net/publication/372051755_Vogelgriep_panzootie_leidt_tot_massastrandingen_van_Jan-van-genten_Morus_bassanus_in_Nederland_april-oktober_2022
Summary of HPAI leading to mass strandings of wild birds in the Netherlands (April – Oct 2022) [In Dutch].
A Comparison of Host Responses to Infection with Wild-Type Avian Influenza Viruses in Chickens and Tufted Ducks
https://journals.asm.org/doi/epub/10.1128/spectrum.02586-22
Differences in immune responses of tufted ducks and chicken to LPAI from Mallards – reveals immune responses in the event of cross species transmission events.
Detection of intercontinental reassortant H6 avian influenza viruses from wild birds in South Korea, 2015 and 2017
https://www.frontiersin.org/articles/10.3389/fvets.2023.1157984/full
Studies of LPAI have been key to helping us how viruses cross geographic boundaries (Antlantic/Pacific Ocean). Recent study finds H6 viruses with combination of genes from America (NP, NS) and Eurasia. Phenomenon usually limited to gulls and HPAI
The Evolution of Highly Pathogenic Avian Influenza A (H5) in Poultry in Nigeria, 2021–2022
https://www.mdpi.com/1999-4915/15/6/1387
467 outbreaks of HPAI in Nigeria from 2021-22, in backyard, semi intensive and intensive operations with mix of chickens and ducks. Two HA clades -Nigerian/European H5N1/N2 & Nigerian/Europe/Asian H5N8. Novel genome constellation in H5N2 fro H5N1+H9N2
Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Domestic Cat, France, 2022
https://wwwnc.cdc.gov/eid/article/29/8/23-0188_article
Case report of HPAI in a domestic cat in France. Cat lived with family next to duck farm, on which HPAI was confirmed and 8375 ducks culled. Neurologic and respiratory (dyspnea) symptoms in cat. Genetically same virus as ducks.
BTN3A3 evasion promotes the zoonotic potential of influenza A viruses
https://www.nature.com/articles/s41586-023-06261-8
Despite plenty of exposure, why are there so few human cases of HPAI? New study shows importance of a human gene BTN3A3 as an inhibitor of avian influenza, but not human influenza. Lots of great threads on this article, including this one: https://twitter.com/SpyrosLytras/status/1674339262894948353?s=20
Flu hits breeding rate of UK’s largest bird of prey
https://www.bbc.com/news/uk-scotland-highlands-islands-66051875
Healthy adults possess cross-reactive neuraminidase inhibition antibodies to an A(H5N1) clade 2.3.4.4b avian influenza virus A/Black Faced Spoonbill/Hong Kong/AFCD-HKU-22-21429-01012/2022
https://www.medrxiv.org/content/10.1101/2023.06.23.23291839v1
Cross reactive NA antibodies against HPAI found in 63 humans in Hong Kong, but no cross reactive HA antibodies. These NA antibodies likely from exposure to human H1N1. Partial protection from season influenza against avian influenza?
Highly Pathogenic Avian Influenza (H5N1) in Humans after the emergence of clade 2.3.4.4b in 2020
https://jglobalbiosecurity.com/articles/10.31646/gbio.218
List of 2.3.4.4b H5N1 cases. Doesn’t delineate bona fide infection from those “Environmental carriers”.
Influenza A in Shorebirds in the Tropical Landscape of Guatemala
https://doi.org/10.1675/063.045.0315
Only 1 seropositive shorebirds, not qPCR positive shorebirds – LPAI
The UK joint Human Animal Infections and Risk Surveillance (HAIRS) group have published a risk assessment
https://www.gov.uk/government/publications/hairs-risk-assessment-avian-influenza-ah5n1-in-non-avian-uk-wildlife/hairs-risk-assessment-avian-influenza-ah5n1-in-non-avian-uk-wildlife
Assessment of the risk of infection in the UK
Probability
General UK population: Very Low
The probability of infection would be considered Low for those exposed to infected live or dead non-avian wildlife.
Impact
The impact on the general UK population would be considered Very Low, while it would be considered Low for high risk groups (for example individuals with occupational exposure to infected wildlife and/or immunocompromised or paediatric cases).
Level of confidence in assessment of risk
Satisfactory.
Newly emerged genotypes of highly pathogenic H5N8 avian influenza viruses in Kagoshima prefecture, Japan during winter 2020/21
https://doi.org/10.1099/jgv.0.001870
Study from Japan addresses HPAI H5N8 in 2020/21, prior to the dominance of H5N1. Detection of G1 in the winter, and G2 only in late winter, suggestion more than one introduction. Lots of evidence for reassortment with locally circulating LPAI.
Highly Pathogenic Avian Influenza Virus (H5N1) Clade 2.3.4.4b Introduced by Wild Birds, China, 2021
https://wwwnc.cdc.gov/eid/article/29/7/22-1149_article
HPAI viruses isolated in China 2021, belonged to G07 (originated E. Asia), and G10(originated Russia) lineage. Viruses were moderately pathogenic in mice but were highly lethal in ducks. Fall in the same antigenic cluster as H5 vaccine used in China.
Genetic Characterization and Pathogenesis of H5N1 High Pathogenicity Avian Influenza Virus Isolated in South Korea during 2021–2022
https://www.mdpi.com/1999-4915/15/6/1403
HPAI caused 47 outbreaks in poultry farms, S. Korea 2021-22, phylogenetically similar to viruses in Europe. When inoculated chickens showed virulent pathogenicity & high mortality. In ducks no mortality, high transmission rates & long viral shedding
Low Susceptibility of Pigs against Experimental Infection with HPAI Virus H5N1 Clade 2.3.4.4b
https://wwwnc.cdc.gov/eid/article/29/7/23-0296_article
Despite the assertion that pigs are “mixing vessels”, and that historically avian lineages circulate in pigs, they arent very susceptible to HPAI. After experimental innoc, only 1 pig seroconverted.
The importance of rapid and robust availability of epidemiological data for real-time mapping of the risk of avian influenza a (H5N1) spread
https://doi.org/10.1080/20477724.2023.2228055
Data accessibility and sharing critical in our response to HPAI. Here a collation of HPAI data from various sources, and easily machine readable. Less comprehensive than WAHIS, but data easier to access (and likely, analyze).
Validation of an RNAscope assay for the detection of avian influenza A virus
https://journals.sagepub.com/doi/epub/10.1177/10406387231182385
New approach to detecting avian influenza from formalin-fixed, paraffin-embedded tissues: RNAScope. Substantially higher sensitivity of detection within positive tissues, but challenging to differential nucleus vs cytoplasmic positives.
Mammalian infections with highly pathogenic avian influenza viruses renew concerns of pandemic potential
https://rupress.org/jem/article/220/8/e20230447/214173/Mammalian-infections-with-highly-pathogenic-avian
Mammalian infections with HPAI renews concerns of pandemic potential. Infection of mammals increases the opportunity for the virus to acquire mutations that enhance efficient infection, replication, and spread in mammals
Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2218945
Comparative pathology of seven HPAI 2.3.4.4 lineages in chickens. Clade 2.3.4.4b viruses showed 100% mortality, but no transmission to co-housed chickens. All the infected chickens died showing systemic infection, irrespective of subgroup.
The first case of human infection with H5N1 avian influenza a virus in Chile
https://pubmed.ncbi.nlm.nih.gov/37310882/
More information about the human HPAI case in Chile. > patient’s residence is located one block from the seashore where seabirds infected with H5N1 viruses had previously been detected. Sequence similar to wild bird viruses in Chile.
Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count
https://journals.asm.org/doi/full/10.1128/spectrum.04351-22?af=R
Despite work on immune systems of chickens, our understanding of the immune response of ducks is poor. A new flow cytometry-based duck WBC differential for quantification of mallard immune cells including B cells, CD4+ T cells, CD8+ cytotoxic T cells
Genetic characterization of a new candidate hemagglutinin subtype of influenza A viruses
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2225645
A new HA sutbype found in birds, related to H9, but only ~60% similar.
Multiple infections with H5N8 subtype high pathogenicity avian influenza viruses in a feral mallard
https://www.jstage.jst.go.jp/article/jvms/advpub/0/advpub_23-0124/_article
Case report of a duck in Asia co-infected with two different genome constellations of HPAI. Of course not surprising, this is a key requirement for reassortment to occur.
Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
https://www.mdpi.com/2076-2607/11/5/1162
Majority of pigs tested, which were in contact with infected birds, were serologically positive for the hemagglutination inhibition test and microneutralization assay.
Efficient and Informative Laboratory Testing for Rapid Confirmation of H5N1 (Clade 2.3.4.4) High-Pathogenicity Avian Influenza Outbreaks in the United Kingdom
https://www.mdpi.com/1999-4915/15/6/1344
APHA have published a new qPCR based assay for confirmation of HPAI.
The Molecular Epidemiology of Clade 2.3.4.4B H5N1 High Pathogenicity Avian Influenza in Southern Africa, 2021–2022
https://www.mdpi.com/1999-4915/15/6/1383
Great summary of HPAI in Southern Africa since 2021. clade 2.3.4.4B H5N1 first found in ZA poultry in April 2021. 7 constellations in initial outbreaks, but by 2022 only 2 remained. 83% poultry outbreaks linked to wild birds
Ecogeographic Drivers of the Spatial Spread of Highly Pathogenic Avian Influenza Outbreaks in Europe and the United States, 2016–Early 2022
https://www.mdpi.com/1660-4601/20/11/6030
Once introduced North America, HPAI spread more rapidly (compared to within Europe). Geographic proximity is a key predictor of virus spread = long distance transport rare. An increase in temperature was predictive of reduced spread.
Rapid evolution of A(H5N1) influenza viruses after intercontinental spread to North America
https://www.nature.com/articles/s41467-023-38415-7
Shows the different phenotypic properties of the many HPAI reassortants in North America. Lots of work in ferrets here.
The first meeting of the Standing Group of Experts on HPAI for Europe
https://rr-europe.woah.org/en/Events/the-first-meeting-of-the-sge-hpai/
> Conclusions and recommendations (draft)
https://rr-europe.woah.org/wp-content/uploads/2023/05/09_draft-recommendations_sge-hpai-1st-meeting_v02.pdf
5. Members’ Veterinary Authorities and WOAH Reference Laboratories for avian influenza exchange information related to the development, testing and use of vaccines against HPAI and modelling activities that inform collective assessment of possible vaccination strategies and policy contributing to ensure that proper vaccination is implemented avoiding use of unreliable vaccines or wrong vaccination strategies ensuring also that surveillance in vaccinated populations is robust and capable of detecting infection with wild-type viruses.
6. Members encourage research institutions and vaccine manufacturers to invest and collaborate on research and development of effective and safe HPAI vaccines adapted to different species of poultry in accordance with the standards in the Terrestrial Manual;
US will vaccinate birds against avian flu for first time — what researchers think
https://www.nature.com/articles/d41586-023-01760-0
Vaccination has been approved for California Condors, comprising the first approval for HPAI vaccines in the US. It took 20 years to help the species recover, the 21 dead birds found has been highly concerning. First trials occurring in Black Vultures.
Avian influenza overview March – April 2023
https://www.ecdc.europa.eu/sites/default/files/documents/Avian-influenza-overview-March-April-2023_0.pdf
update from EFSA: 106 domestic and 613 wild bird outbreaks across 24 countries. On going outbreaks in Black-headed Gulls
High number of HPAI H5 Virus Infections and Antibodies in Wild Carnivores in the Netherlands, 2020-2022
https://www.biorxiv.org/content/10.1101/2023.05.12.540493v1.full.pdf
Testing of 500 dead mammals in the Netherlands: virological evidence for HPAI infection in 0.8% (2020), 1.4%(2021), 9.9% (2022). highest prevalence in foxes, polecats and stone martens. 20% seropositive. PB2 mutations found in viral genomes.
Highly pathogenic avian influenza causes mass mortality in Sandwich tern (Thalasseus sandvicensis) breeding colonies across northwestern Europe
https://www.biorxiv.org/content/10.1101/2023.05.12.540367v1.full.pdf
Sandwich Terns in Europe absolutely devastated by HPAI last year, with most colonies affected. “20,531 adult Sandwich terns were found dead, which is >17% of the total northwestern European breeding population. Inside the colonies almost all chicks died”
Multiple introductions of highly pathogenic avian influenza H5N1 clade 2.3.4.4b into South America
https://doi.org/10.1016/j.tmaid.2023.102591
More evidence of at least 4 incursions into South America.
Novel Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Wild Birds, South Korea
https://wwwnc.cdc.gov/eid/article/29/7/22-1893_article
Additional diversity in genome constellations found in wild birds in South Korea in 2022, with some evidence of similarity to the “G10” constellation [reassortant of HPAI and PB2 LPAI) found in China in 2022/23
Extensive Diversity and Evolution of Highly Pathogenic Avian Influenza A (H5) in Poultry in Nigeria, 2021-2022
https://www.preprints.org/manuscript/202305.1027/v1
Between 2021-22, 467 outbreaks of HPAI detected in Nigeria. Widespread distribution of the H5Nx clade 2.3.4.4b and similarity with the HPAI H5Nx viruses detected in Europe since late 2020. Detection of reassortant with H9N2.
Novel Avian Influenza Virus (H5N1) Clade 2.3.4.4b Reassortants in Migratory Birds, China
https://wwwnc.cdc.gov/eid/article/29/6/22-1723_article
HPAI viruses from swans in China in 2021 fall into 2.3.4.4b2 clade, and have unique genome constellations. Spread analysis suggests internal segments originally from Africa, via Europe to China.
Spatio-temporal analysis of Highly Pathogenic Avian Influenza HPAI (H5N1) in poultry in Menofia governorate, Egypt
https://www.researchsquare.com/article/rs-2948767/v1
Tracking of HPAI in Egypt from 2006-2017 demonstrates 6 waves through poultry. Includes clade 2.2.1.1a, 2.2.1.2. Rural districts and villages key hotspots. Challenging to get data due to lack of notification, effects of vaccination, compensation issues.
Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
https://repo.niv.ns.ac.rs/xmlui/handle/123456789/614
Outbreak report of HPAI H5N8 in 2016, and recently H5N1 in Serbia in 2021/22. Following outbreaks, strict control measures were implemented on farms and backyard holdings to prevent the occurrence and spread of the disease.
Surveillance of avian influenzas viruses from 2014 to 2018 in South Korea
https://www.nature.com/articles/s41598-023-35365-4
Study on LPAI in Korea between 2014-2018. Apparently not HPAI detected at all.
An amplicon-based nanopore sequencing workflow for rapid tracking of avian 2 influenza outbreaks, France, 2020-2022
https://www.biorxiv.org/content/10.1101/2023.05.15.538689v1.full.pdf
Real time, field based protocol for rapid HPAI sequencing in the field. Probably very useful for us to have in the back pocket.
Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus
https://journals.asm.org/doi/full/10.1128/spectrum.04946-22
Real time, field based protocol for rapid HPAI sequencing in the field. Looks like it can work for more than just H5N1 though.
A real-time colorimetric reverse transcription loop-mediated isothermal 2 amplification (RT-LAMP) assay for the rapid detection of highly pathogenic H5 3 clade 2.3.4.4b avian influenza viruses
https://www.biorxiv.org/content/10.1101/2023.05.14.540682v1.full.pdf
Rapid detection of HPAI with LAMP. “specific detection of HPAIV H5Nx clade 2.3.4.4b within 30 minutes with a sensitivity of 86.11%” .
Avian influenza, new aspects of an old threat
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.19.2300227?crawler=true
“To tackle the threat of avian influenza, a One Health approach is needed through rapid sharing of information about outbreaks, provision of sequence data and reference viruses, and close collaboration between the different sectors locally and globally.”
Epidemiology and molecular characterization of avian influenza A viruses H5N1 and H3N8 subtypes in poultry farms and live bird markets in Bangladesh
https://www.nature.com/articles/s41598-023-33814-8
Avian influenza prevalence in LBMs in Bangladesh comprising 40.20% – 52.38% in chicken, 46.96% in waterfowl, 31.1% in turkey. H5 comprised the highest prevalence: clade 2.3.2.1.a (circulating since 2015). H3N8 viruses similar to those in China.
Epidemiological Distribution of respiratory viral pathogens in marketable vaccinated broiler chickens in six governorates in the Nile Delta, Egypt, January to October 2022
https://www.researchsquare.com/article/rs-2944417/v1
In 2022, 293/359 poultry flocks tested positive for respiratory viruses in Egypt. NDV found to be the most common, followed by IBV, H9 and H5. Lots of co-infections.
High proportion of H3 avian influenza virus circulating in chickens-an increasing threat to public health
https://www.sciencedirect.com/science/article/pii/S0163445323002967
Reanalysis of H3N8 show human and poultry cases in China form a monophyletic clade. Surveillance data for the past three years: H3 widely distributed in many provinces of China, with prevalence of ~60% in chickens, 31% in ducks, 7% in pigeons.
Synchrony of Bird Migration with Avian Influenza Global Spread; 2 Implications for Vulnerable Bird Orders
https://www.biorxiv.org/content/10.1101/2023.05.22.541648v1.full.pdf
Seasonal bird migration can explain salient features of the global dispersal of 2.3.4.4. – differing vulnerable bird orders at geographical origins and destinations of HPAIV H5 lineage movements – role of Suliformes and Ciconiformes
Mixed selling of different poultry species facilitates emergence of public-health-threating avian influenza viruses
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2214255
“mixed poultry selling at retail live poultry markets has increased the genetic diversity of AIVs, which might facilitate the emergence of novel viruses that threaten public health”
Antigenic mapping of the hemagglutinin of the H9 subtype influenza A viruses using sera 2 from Japanese quail (Coturnix c. japonica).
https://www.biorxiv.org/content/10.1101/2023.05.18.541344v1.full.pdf
Vaccination against H9 is widely employed in the MiddleEast/Asia. New antigenic maps have now been produced, with important implications for understanding antigenic drift and improving vaccine development and use.
The bat-borne influenza A virus H9N2 exhibits a set of unexpected pre-pandemic features
https://www.researchsquare.com/article/rs-2937503/v1
H9N2 influenza A virus in Egyptian bat exhibits high replication and transmission potential in ferrets, efficient infection of human lung cultures and escape from the antiviral activity of MxA. = criteria for pre-pandemic virus.
High pathogenicity avian influenza (H5N1) in Northern Gannets: Global spread, clinical signs, and demographic consequences
https://www.biorxiv.org/content/10.1101/2023.05.01.538918v1
In 2022, unusually high mortality was detected in 75% of Northern Gannet colonies, globally, with HPAI confirmed in 58% of cases. Decreased adult survival and breeding success. Some birds survived – with black irises.
Remote Sensing and Ecological Variables Related to Influenza a Prevalence and Subtype Diversity in Wild Birds in the Lluta Wetland of Northern Chile
https://www.preprints.org/manuscript/202305.0199/v1
AIV prevalence is dictacted by numerous factors. New study from Chile show diversity of LPAI in the Lluta River wetland, and that prevalence correspond to Normalized Difference Vegetation Index, abundance of migratory birds
Circulation of highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b in highly diverse wild bird species from Peru
https://www.researchsquare.com/article/rs-2814674/v1
More HPAI data from Peru. Viral isolates and sequences from pelicans, gulls, cormorants, penguins, and an array of raptors. Viruses similar to those from Chile.
Development of a nucleoside-modified mRNA vaccine against clade 2.3.4.4b H5 highly pathogenic avian influenza virus
https://www.biorxiv.org/content/10.1101/2023.04.30.538854v1.full.pdf
Development of an mRNA vaccine against HPAI, although targetted towards mammals, with the vaccine immunogenic in mice and ferrets and prevents morbidity and mortality of ferrets following challenge.
Wild birds’ plight and role in the current bird flu panzootic
https://www.biorxiv.org/content/10.1101/2023.05.02.539182v1
Influenza A viruses in gulls in landfills and freshwater habitats in Minnesota, United States
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10203411/pdf/fgene-14-1172048.pdf
Nice study on H13, including AIV work and tracking and migration stuff.
HPAIV outbreak triggers long-distance movements in breeding Northern gannets — implications for disease spread
https://www.authorea.com/users/607294/articles/636040-hpaiv-outbreak-triggers-long-distance-movements-in-breeding-northern-gannets-implications-for-disease-spread?commit=554c9f0238d40f35cddaddcf16dce1ed97f98543
Environmental Samples Test Negative for Avian Influenza Virus H5N1 Four Months after Mass Mortality at A Seabird Colony
https://www.mdpi.com/2076-0817/12/4/584
At Foula, Shetland, 1500 breeding adult great skuas Stercorarius skua, totalling about two tonnes of decomposing virus-laden material, died at the colony in May−July 2022… No viral genetic material was detected four months after the mortality, suggesting a low risk of seabird infection from the local environment when the seabirds would return the next breeding season
This is in contrast to studies of LPAI that found infectious virus for almost a year!
For example: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542776/
Viruses: How avian influenza viruses spill over to mammals
https://elifesciences.org/articles/86051
Lovely summary of the recent canine influenza paper, relevant in demonstrating that avian viruses may emerge and adapt to mammals.
Detection and Genomic Characterization of an Avian Influenza Virus Subtype H5N1 (Clade 2.3.4.4b) Strain Isolated from a Pelican in Peru
https://journals.asm.org/doi/10.1128/mra.00199-23
Short note about the sequencing of a single viral isolate using nanopore.
Comprehensive analysis of the key amino acid substitutions in the polymerase and NP of avian influenza virus that enhance polymerase activity and affect adaptation to mammalian hosts
https://www.sciencedirect.com/science/article/pii/S0378113523001128
More efforts to map mutations required for avian influenza viruses to infect mammals, focussing on genetic screens of internal segments and experiments with H7. Mutations other than PB2 E627K at play.
Highly Pathogenic H5 Influenza Viruses Isolated between 2016 and 2017 in Vietnamese Live Bird Markets
https://www.mdpi.com/1999-4915/15/5/1093
# 2344f. 2344g and 2321c
Potential Effects of Habitat Change on Migratory Bird Movements and Avian Influenza Transmission in the East Asian-Australasian Flyway
https://www.mdpi.com/1424-2818/15/5/601
Modelling and telemetry shows that minor changes in land-use in China may have large ramifications for stopover sites, and thus HPAI risk.
Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus
https://www.mdpi.com/2076-2607/11/5/1162
Pigs reared on same property as infected birds showed extensive seroconversion to 2.3.4.4b: 61% positive using HI and of 9 tested using MN, all positive?. Raises questions about whether all pigs exposed to infected birds/dust/particles or transmission?
Transmission of lethal H5N1 clade 2.3.4.4b avian influenza in ferrets
https://www.researchsquare.com/article/rs-2842567/v1
Here we show that multiple naturally circulating H5N1 viruses can replicate in primary human airway epithelial cells and cause lethal disease in multiple mammalian species. One isolate, A/Red Tailed Hawk/ON/FAV-0473-4/2022, efficiently transmitted by direct contact between ferrets, resulting in lethal outcomes…. uncharacterized, genetic signatures may be important determinants of mammalian adaptation and pathogenicity of these viruses
Replication of Novel Zoonotic-Like Influenza A(H3N8) Virus in Ex Vivo Human Bronchus and Lung
https://wwwnc.cdc.gov/eid/article/29/6/22-1680_article
Virus has affinity for both α-2,3 and α-2,6, but inefficient replication in human bronchial tissues and has limited efficiency for human-to-human transmission
Risk for Infection in Humans after Exposure to Birds Infected with Highly Pathogenic Avian Influenza A(H5N1) Virus, United States, 2022
https://wwwnc.cdc.gov/eid/article/29/6/23-0103_article
Of 4,000 persons exposed to HPAI H5N1–infected birds, only 1 has ever had qPCR confirmed case. This person was unlikely actually infected, but rather was a case of putative environmental contamination.
Identification of a duck H9N2 influenza virus possessing tri-basic hemagglutinin cleavage sites genetically close to the human H9N2 isolates in China, 2022
https://doi.org/10.1016/j.jinf.2023.04.007
Detection of an H9N2 virus in ducks in China, linked to ongoing human cases, and with a tribasic PSRSRR/GLF motif and shows a preference for binding to α-2,6 human-like receptors
Spatio-temporal dynamics and drivers of Highly Pathogenic Avian Influenza H5N1 in Chile
https://www.biorxiv.org/content/10.1101/2023.04.24.538139v1.full.pdf
Since 7 Dec 2022, Chile has reported 197 HPAI outbreaks, with 478 individual cases in birds, and 14 statistically significant clusters including a poultry production centre, and Tocopilla (human case here). Wave-like spread from north to south.
Phylogenetic analysis reveals that the H5N1 avian influenza A outbreak in poultry in Ecuador in November 2022 is associated to the highly pathogenic clade 2.3.4.4b
https://doi.org/10.1016/j.ijid.2023.04.403
In Nov 2022, HPAI was first detected in Ecuador. By Feb 2023 1.1 million poultry were culled. Virus similar to those from Peru and Chile and closely related to sequences from Venezuelan pelicans.
Interventions to Reduce Risk for Pathogen Spillover and Early Disease Spread to Prevent Outbreaks, Epidemics, and Pandemics
https://wwwnc.cdc.gov/eid/article/29/3/22-1079_article
PAHO seeks to strengthen regional avian influenza surveillance and response [16 Mar 2023] PAHO = The Pan American Health Organization
HPAI in Great Britain: evaluation and future actions | Gov.uk 30 March 2023
“The HPAIG [Scientific Advisory Group in highly pathogenic avian influenza] was tasked with addressing four key issues with regards to the current epidemic of HPAI in Great Britain (England, Scotland and Wales)
– the host range of the current virus and their potential roles
– the possibility of interventions to reduce impact on wild birds
– the potential to supplement current approaches to control with vaccination
– the potential to model the expected future trajectory of the outbreak
Evolution of Avian Influenza Virus (H3) with Spillover into Humans, China
https://wwwnc.cdc.gov/eid/article/29/6/22-1786_article
Human Infection with highly pathogenic avian influenza A(H5N1) virus in Chile
https://www.cdc.gov/flu/avianflu/spotlights/2022-2023/chile-first-case-h5n1-addendum.htm
## this is an addendum to a technical report: https://www.cdc.gov/flu/avianflu/spotlights/2022-2023/h5n1-technical-report.htm
NatureScot Scientific Advisory Committee Sub-Group on Avian Influenza Report on the H5N1 outbreak in wild birds 2020-2023
https://www.nature.scot/doc/naturescot-scientific-advisory-committee-sub-group-avian-influenza-report-h5n1-outbreak-wild-birds
Bayesian phylodynamics reveals the transmission dynamics of avian influenza A(H7N9) virus at the human–live bird market interface in China
https://www.pnas.org/doi/10.1073/pnas.2215610120
Efficacy of multivalent recombinant herpesvirus of turkey vaccines against high pathogenicity avian influenza, infectious bursal disease, and Newcastle disease viruses
https://www.sciencedirect.com/science/article/pii/S0264410X23003493
Emergence and rapid dissemination of highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b in wild birds, Chile
https://www.biorxiv.org/content/10.1101/2023.04.07.535949v1
Mass mortality among colony-breeding seabirds in the German Wadden Sea in 2022 due to distinct genotypes of HPAIV H5N1 clade 2.3.4.4b
https://pubmed.ncbi.nlm.nih.gov/37014781/
Targeted genomic sequencing of avian influenza viruses in wetlands sediment from wild bird habitats
https://www.biorxiv.org/content/10.1101/2023.03.30.534984v1.abstract
The pathogenesis of a 2022 North American highly pathogenic clade 2.3.4.4b H5N1 avian influenza virus in mallards (Anas platyrhynchos)
https://www.tandfonline.com/doi/abs/10.1080/03079457.2023.2196258?journalCode=cavp20
Using integrated wildlife monitoring to prevent future pandemics through one health approach
https://www.sciencedirect.com/science/article/pii/S2352771422001112
41% of Pelicans in Peru now dead due to HPAI
https://www.actualidadambiental.pe/el-41-de-la-poblacion-de-pelicanos-ha-muerto-desde-el-inicio-de-la-gripe-aviar-en-peru/
Prevalence, evolution, replication and transmission of H3N8 avian influenza viruses isolated from migratory birds in eastern China from 2017 to 2021
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2184178
## highly relevant given the recent human case of avian H3
H9N2 avian influenza virus dispersal along Bangladeshi poultry trading networks
https://academic.oup.com/ve/article/9/1/vead014/7057897?login=false
Human influenza A virus H1N1 in marine mammals in California, 2019
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283049
Gradual adaptation of animal influenza A viruses to human-type sialic acid receptors
https://www.sciencedirect.com/science/article/pii/S1879625723000147
The role of airborne particles in the epidemiology of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus in commercial poultry production units
https://www.biorxiv.org/content/10.1101/2023.03.16.532935v1
Avian influenza H5N1 in a great white pelican (Pelecanus onocrotalus), Mauritania 2022
https://link.springer.com/article/10.1007/s11259-023-10100-6
Welcome on EURL Avian Flu Data Portal
https://eurlaidata.izsvenezie.it/
The changing dynamics of highly pathogenic avian influenza H5N1: Next steps for management & science in North America
https://doi.org/10.1016/j.biocon.2023.110041
Active wild bird surveillance of avian influenza viruses, a report
https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/sp.efsa.2022.EN-7791
It outlines active surveillance designs, movement informed site selections, integration and analyses of ornithological data, practicalities of field sampling, virus informed epidemiology, climate and bird migration data etc. Obviously from a European lens, but Im sure there are still lots of good lessons tucked in here.
H7N9 influenza A virus transmission in a multispecies barnyard model
https://www.sciencedirect.com/science/article/abs/pii/S0042682223000764
Protective efficacy of a bivalent H5 influenza vaccine candidate against both clades 2.3.2.1 and 2.3.4.4 high pathogenic avian influenza viruses in SPF chickens
https://www.sciencedirect.com/science/article/pii/S0264410X23003110
How human ecology landscapes shape the circulation of H5N1 avian influenza: A case study in Indonesia
https://www.sciencedirect.com/science/article/pii/S2352771423000575
Highly pathogenic avian influenza (HPAI A H5N1) outbreak in Spain: its mitigation through the One Health approach – a short communication
https://journals.lww.com/annals-of-medicine-and-surgery/Abstract/9900/Highly_pathogenic_avian_influenza__HPAI_A_H5N1_.195.aspx
A protective measles virus-derived vaccine inducing long-lasting immune responses against influenza A virus H7N9
https://www.nature.com/articles/s41541-023-00643-9
Statistical Analysis of the Performance of Local Veterinary Laboratories in Molecular Detection (rRT-PCR) of Avian Influenza Virus via National Proficiency Testing Performed during 2020–2022
https://www.mdpi.com/1999-4915/15/4/823
Letter to the editor: Highly pathogenic influenza A(H5N1) viruses in farmed mink outbreak contain a disrupted second sialic acid binding site in neuraminidase, similar to human influenza A viruses
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.7.2300085;jsessionid=YScPi6NkUoXrgPSFgxKwpMTyCSlRWt6lNkuGTx7q.i-0b3d9850f4681504f-ecdclive
This is a response to the original mink paper.. there appears to be some discussion happening about what all the mutations they found mean.
Characterization of an H7N9 Influenza Virus Isolated from Camels in Inner Mongolia, China
https://journals.asm.org/doi/epub/10.1128/spectrum.01798-22
Whole-genome sequence and genesis of an avian influenza virus H5N1 isolated from a healthy chicken in a live bird market in Indonesia: accumulation of mammalian adaptation markers in avian hosts
https://peerj.com/articles/14917/
Influenza A(H5N1) detection in two asymptomatic poultry farm workers in Spain, September to October 2022: suspected environmental contamination
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.8.2300107?TRACK=RSS
Letter to the Editor: Knowledge gap in assessing the risk of a human pandemic via mammals’ infection with highly pathogenic avian influenza A(H5N1)
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.9.2300134?crawler=true
Characterization of neurotropic HPAI H5N1 viruses with novel genome constellations and mammalian adaptive mutations in free-living mesocarnivores in Canada
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2186608
Infection of wild rats with H5N6 subtype highly pathogenic avian influenza virus in China
https://www.journalofinfection.com/article/S0163-4453(23)00136-6/fulltext
First case of human infection with highly pathogenic H5 avian influenza a virus in South America: a new zoonotic pandemic threat for 2023?
https://pubmed.ncbi.nlm.nih.gov/36881656/
Being ready for the next influenza pandemic?
https://www.thelancet.com/journals/laninf/article/PIIS1473-3099(23)00117-2/fulltext
Virological and Genetic Characterization of the Unusual Avian Influenza H14Nx Viruses in the Northern Asia
https://www.mdpi.com/1999-4915/15/3/734
The H14 story continues, with detections in Russian waterfowl.
Reasons for low prevalence? Perhaps waterfowl detections are only spillovers from other bird groups, not included in AIV surveillance?
Evolution of highly pathogenic H5N1 influenza A virus in the central nervous system of ferrets
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011214
Given most mammals infected with HPAI are showing neurological signs, this study is timely in showing that HPAI can enter the CNS of mammals and undergo positive selection while there
Infection of wild rats with H5N6 subtype highly pathogenic avian influenza virus in China
https://www.sciencedirect.com/science/article/pii/S0163445323001366
via metagenomics HPAI H5N6 found in rats in China. Rats may have been exposed from local poultry market. SNPs for mammalian infection detected.
Bona fide infection, or detection of HPAI in diet following eating infected poultry scraps?
First case of human infection with highly pathogenic H5 avian Influenza A virus in South America: A new zoonotic pandemic threat for 2023?
https://academic.oup.com/jtm/advance-article/doi/10.1093/jtm/taad032/7070564?login=false
More details now available pertaining to human case of HPAI in Ecuador in Dec 2022. Girl was in contact with sick and dead backyard poultry. Unfortunately no genome sequences generated and there was limited surveillance in humans or birds at the time
Characterization of neurotropic HPAI H5N1 viruses with novel genome constellations and mammalian adaptive mutations in free-living mesocarnivores in Canada
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2186608
Summary of mammalian cases of HPAI in Canada.
-Mammals were infected with wholly Eurasian viruses, but also EU-NA reassorted viruses.
-17% had PB2 mutation, other mammalian adapted mutations also found.
-Some Red Foxes survived infection.
Whole-genome sequence and genesis of an avian influenza virus H5N1 isolated from a healthy chicken in a live bird market in Indonesia: accumulation of mammalian adaptation markers in avian hosts
https://peerj.com/articles/14917/
Great to see some HPAI genomes from Indonesia, albeit lineage 2.3.2.1c, and not the same as 2.3.4.4b. Reassortment between 2.3.2.1c, and H3 virus, and an H5N1 Indonesian endemic virus
Characterization of an H7N9 Influenza Virus Isolated from Camels in Inner Mongolia, China
https://journals.asm.org/doi/epub/10.1128/spectrum.01798-22
New report of H7N9 avian influenza in camels. Other than humans (2013-2017), H7N9 has never caused mammalian infections (that we know of). The virus had mammalian adaptations (e.g. PB2 mutation), higher SA-α2 binding and mammalian cell replication.
Comparative Analysis of Different Inbred Chicken Lines Highlights How a Hereditary Inflammatory State Affects Susceptibility to Avian Influenza Virus
https://www.mdpi.com/1999-4915/15/3/591
Chickens in industrial poultry farming are highly inbred.
Different chicken lines, respond differently to avian influenza infection. Useful to understand immunity. Also, we should better consider infection resistance in selecting/breeding chicken lines.
Pathology of natural infection with highly pathogenic avian influenza virus (H5N1) clade 2.3.4.4b in wild terrestrial mammals in the United States in 2022
https://www.biorxiv.org/content/10.1101/2023.03.10.532068v1
Great summary outlining pathological findings of 62 dead mammals infected with HPAI.
“Infected mammals primarily exhibited neurological signs. Necrotizing meningoencephalitis, interstitial pneumonia, and myocardial necrosis were the most common lesions”
Highly pathogenic avian influenza A (H5N1) virus infections in wild carnivores connected to mass mortalities of pheasants in Finland
https://www.sciencedirect.com/science/article/pii/S1567134823000217?via%3Dihub
Great study linking mammalian cases of HPAI in Finland to an outbreak in farmed and released pheasants.
“avian influenza cases in mammals were spatially and temporally connected with avian mass mortalities”
Highly pathogenic avian influenza in wild birds in the United Kingdom in 2022: impacts, planning for future outbreaks, and conservation and research priorities.
Report on virtual workshops held in November 2022
https://www.bto.org/sites/default/files/publications/rr752_pearce-higgins_et_al_2023_hpai_workshop_final_web_0.pdf
Great resource from the BTO on impacts, planning for future outbreaks, and conservation and research priorities in regards to HPAI
Bird flu can jump to mammals – should we worry?
https://www.sciencenews.org/article/bird-flu-mammals-influenza-pandemic
Avian influenza spread and seabird movements between colonies
https://doi.org/10.1016/j.tree.2023.02.002
How does avian influenza spread within and between seabird colonies? Seabird movements such as dispersal, prospecting, foraging and migration in addition to direct contact certainly at play
Descriptive Epidemiology of and Response to the High Pathogenicity Avian Influenza (H5N8) Epidemic in South African Coastal Seabirds, 2018
https://www.hindawi.com/journals/tbed/2023/2708458/
Unlike the Northern Hem which experienced large scale outbreaks of HPAI in seabirds in 2022 for the first time, S. African seabirds have experienced re-occuring outbreaks for many years now. Many lessons we can learn from their experiences.
Influenza A(H5N1) detection in two asymptomatic poultry farm workers in Spain, September to October 2022: suspected environmental contamination
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2023.28.8.2300107#html_fulltext
The scale of HPAI outbreaks is huge.
This figure demonstrates the number of outbreaks in Spain alone, including wild birds, poultry, mink, and most recently 2 putative human cases (although the latter may be environmental contamination…)
Blue-Winged Teals in Guatemala and Their Potential Role in the Ecology of H14 Subtype Influenza a Viruses
https://www.mdpi.com/1999-4915/15/2/483
The cool story of H14 (a relatively rare influenza subtype) in teals of Guatemala continues.
The high level of H14 in Guatemala may be due to epizootic events from a single introduction, followed by local clonal expansion followed by maintenance
Dynamic Evolution of Avian RNA Virus Sensors: Repeated Loss of RIG-I and RIPLET
https://www.mdpi.com/1999-4915/15/1/3
RIG-I is part of the first line of defense against avian influenza infection, and while present in ducks is missing in chickens. A screen of the avian tree of life indicates absence in other avian families too (penguins, shearwaters)
Pathology of naturally acquired high pathogenicity avian influenza virus H5N1 infection in seabirds
https://www.biorxiv.org/content/10.1101/2023.02.17.528990v1
Pathology studies of HPAI in seabirds. “Across the birds, epitheliotropism was evident… This was, in contrast, not observed in the 2021 summer mortality event in great skuas and may be significant for the disease epidemiology observed in 2022”
Waterfowl recently infected with low pathogenic avian influenza exhibit reduced local movement and delayed migration
https://esajournals.onlinelibrary.wiley.com/doi/10.1002/ecs2.4432
Revisiting the cost of infection of low path influenza – affect of infection on migration.
Telemetry of 165 individuals of 4 species of duck. Antibody pos NOPI = 12 days stopovers. PCR pos Canvasback delayed migration 28 days.
Wild Bird Densities and Landscape Variables Predict Spatial Patterns in HPAI Outbreak Risk across The Netherlands
https://edepot.wur.nl/569551
What predicts when and where HPAI outbreaks will occur?
Wild bird densities and landscape variables (which generally correlate with higher bird densities such as rivers) are key.
Experimental and natural infections of white-tailed sea eagles (Haliaeetus albicilla) with high pathogenicity avian influenza virus of H5 subtype
https://www.frontiersin.org/articles/10.3389/fmicb.2022.1007350/full
In addition to reservoirs (like ducks), raptors are also suffering wide-spread mortality due to HPAI (likely from eating infected ducks). This study further shows the capacity for transmission between raptors of 2.3.4.4e
Zoonotic Mutation of Highly Pathogenic Avian Influenza H5N1 Virus Identified in the Brain of Multiple Wild Carnivore Species
https://www.mdpi.com/2076-0817/12/2/168
Zoonotic mutation PB2-E627K of HPAI H5N1 identified in the brain of multiple wild carnivore species: fox, polecat, otter, and badger
Highly Pathogenic Avian Influenza H5N1 Virus Infections in Wild Red Foxes (Vulpes vulpes) Show Neurotropism and Adaptive Virus Mutations
https://journals.asm.org/doi/10.1128/spectrum.02867-22
Continued concern about mammalian adaption in HPAI (given ? mammalian cases and Spanish mink). An interrogation of viruses found in foxes in the NL – presence of E627K PB2 mutation. Genomic surveillance of HPAI in mammals continues to be very important
First Mass Mortality of Marine Mammals Caused by Highly Pathogenic Influenza Virus (H5N1) in South America
https://www.biorxiv.org/content/10.1101/2023.02.08.527769v1.full.pdf
Lots of HPAI activity in S America, with >50,000 wild birds dead, a human case, and now a mass mortality event in sea lions (634 animals)
Extremely concerning, demonstrates the potential scale of outbreaks that may occur if this virus enters Australia
Clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus (HPAIV) from the 2021/22 epizootic is highly duck adapted and poorly adapted to chickens
https://www.biorxiv.org/content/10.1101/2023.02.07.527270v1.full.pdf
Lots of questions abt why we have seen 2.3.4.4 explode in birds since 2021.
This study argues that virus is highly duck adapted (?transmission & environ contamin) and poorly chicken adapted. Evidence to the idea that wild birds are now a reservoir.
Statement on avian influenza and mammals
https://www.woah.org/en/statement-on-avian-influenza-and-mammals/
The World Organisation for Animal Health have released a statement on avian influenza.
In short: surveillance, prevention, control, protection, monitoring, reporting and SHARING genetic sequence (hopefully in a timely manner).
No evidence for HPAI H5N1 2.3.4.4b incursion into Australia in 2022
https://onlinelibrary.wiley.com/doi/10.1111/irv.13118
The highest risk period for HPAI incursion into Australia is when the migratory birds arrive in the spring. Here
we report the results of our surveillance of migratory birds from Sept-Dec 2022. No HPAI detected.
An Evaluation of Avian Influenza Virus Whole Genome Sequencing Approaches Using Nanopore Technology
https://www.preprints.org/manuscript/202301.0480/v1
Great to see further development of Nanopore sequencing for HPAI.
Unlike SARS-CoV-2, influenza is segmented, so a slightly different approach is warranted.
Consequences and global risks of highly pathogenic avian influenza outbreaks in poultry in the United Kingdom
https://www.ijidonline.com/article/S1201-9712(23)00029-2/fulltext
Consequences of HPAI infection in the UK
– higher risk of human infections
– 4.6 million birds culled 1 Oct – 13 Jan 2023
– egg shortage + ? egg $
– end of free range?
Detection and Phylogenetic Analysis of Highly Pathogenic A/H5N1 Avian Influenza Clade 2.3.4.4b Virus in Chile, 2022
https://www.biorxiv.org/content/10.1101/2023.02.01.526205v1
Increased viral prevalence of avian influenza in Chile corresponded with the arrival of migratory birds at the end of 2022, and HPAI was detected in seabirds: Pelicans, gulls, terns and skimmers. Genomes similar to poultry & wild bird viruses in S. Am.
Homo- and Heterosubtypic Immunity to Low Pathogenic Avian Influenza Virus Mitigates the Clinical Outcome of Infection with Highly Pathogenic Avian Influenza H5N8 Clade 2.3.4.4.b in Captive Mallards (Anas platyrhynchos)
https://www.mdpi.com/2076-0817/12/2/217
Why do some ducks have asymptomatic infections with HPAI H5? Pre-exposure to LPAI H5!
“The mallards pre-exposed to LPAIV H5N1 .. were asymptomatic and showed a significant reduction of viral RNA shedding, .. no disease.. antigen not detected in organs”
Bidirectional Movement of Emerging H5N8 Avian Influenza Viruses Between Europe and Asia via Migratory Birds Since Early 2020
https://academic.oup.com/mbe/article/40/2/msad019/7005671?login=false
Lovely analysis demonstrating bidirectional movement of HPAI H5N8 avian influenza viruses between Europe and Asia. Specifically that temporal estimates from virology data match bird migration timing
Avian influenza leads to mass mortality of adult Great Skuas in Foula in summer 2022
https://www.researchgate.net/publication/367268355_Avian_influenza_leads_to_mass_mortality_of_adult_Great_Skuas_in_Foula_in_summer_2022/references
Summary of mass mortality event of Great Skuas on Foula in summer 2022 due to avian influenza.
“a decline in the order of magnitude of 60–70% in occupied territories is more likely”
Migratory patterns of two major influenza virus host species on tropical islands
https://www.biorxiv.org/content/10.1101/2023.01.18.524666v1
Interrogating how avian influenza is maintained and transmitted between Indian ocean islands. Also reinforces the role of Noddies as hosts for AIV.
Combines bird tracking data and influenza data.
Avian flu threatens Neotropical birds
https://www.science.org/doi/10.1126/science.adg2271
– HPAIv killed more than 22,000 wild birds in just 4 weeks in 2022 in Peru
– December 2022, the virus had been found in birds in Ecuador, Colombia, Venezuela, and Chile
https://science.org/doi/10.1126/science.adg2271
In Vitro and In Vivo Characterization of H5N8 High-Pathogenicity Avian Influenza Virus Neurotropism in Ducks and Chickens
https://journals.asm.org/doi/10.1128/spectrum.04229-22
Why are there differences in disease between chickens and ducks infected with 2.3.4.4b HPAI?
Ducks better control replication in their lungs, so limited respiratory signs & eventual neurological signs. Chickens succumbed to initial resp infections.
Strong host phylogenetic and ecological effects on host competency for avian influenza in Australian wild birds
https://royalsocietypublishing.org/doi/10.1098/rspb.2022.2237
Our latest study showing the role of host phylogeny (and ecological effects as previously shown) on host competency for avian influenza is finally out
Culmination of MANY years of sampling to reveal avian influenza dynamics in Australia
Highly pathogenic avian influenza H5N1 virus outbreak among Cape cormorants (Phalacrocorax capensis) in Namibia, 2022
https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2167610
In January 2022, more than 6500 Cape cormorants died due to HPAI on Bird Island, Walvis Bay. Phylogenetics indicate clade 2.3.4.4b, and highly similar to H5N1 in chickens in Lesotho in May 2021 and poultry and wild birds in Botswana in June 2021
Detection of Clade 2.3.4.4b Avian Influenza A(H5N8) Virus in Cambodia, 2021
https://wwwnc.cdc.gov/eid/article/29/1/22-0934_article
Avian influenza H5 viruses have been routinely detected in Cambodia for a number of years, particularly 2.3.2.1c since 2014. Here 2.3.4.4b was first detected in late 2021.
Emergence of Highly Pathogenic Avian Influenza A Virus (H5N1) of Clade 2.3.4.4b in Egypt, 2021–2022
https://www.mdpi.com/2076-0817/12/1/90
Overview of avian influenza H5N1 in Egypt. Overall, the Egyptian strains shared genetic traits, markers associated with mammalian adaption, and virulence traits similar to those found in HPAI H5N1 strains detected in Europe and Africa
Bald eagle mortality and nest failure due to clade 2.3.4.4 highly pathogenic H5N1 influenza a virus
https://www.nature.com/articles/s41598-023-27446-1
Avian influenza having considerable effects on wild birds beyond waterfowl.
“infection manifested beyond the scale of individual eagles, and directly affected population recruitment dynamics through elevated rates of reproductive failures.”
Pathogenicity of highly pathogenic avian influenza H5N8 subtype for herring gulls (Larus argentatus): impact of homo- and heterosubtypic immunity on the outcome of infection
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-022-01125-x
Gulls are highly susceptible to 2.3.4.4b HPAI, although birds with previous exposure to LPAI H5 fared considerably better than naive birds. Birds previously exposed to H13 (gull specific influenza) faired almost as well as those pre-exposed to LPAI H5
Iceland as Stepping Stone for Spread of Highly Pathogenic Avian Influenza Virus between Europe and North America
https://wwwnc.cdc.gov/eid/article/28/12/22-1086_article
Another paper interrogating the introduction of HPAI from Europe into N.America – this one with data from Iceland.
Rapid evolution of A(H5N1) influenza viruses after intercontinental spread to North America
https://www.researchsquare.com/article/rs-2136604/v1
Different genotypes of 2.3.4.4b H5N1 isolated in North America are phenotypically diverse, with many causing severe disease with dramatic neurologic involvement, in mammals. Reassortment following introduction to N. Am a key driver of genetic change.
Great Skuas and Northern Gannets on Foula, summer 2022 – an unprecedented, H5N1 related massacre
https://dataverse.nioz.nl/file.xhtml?fileId=2735&version=4.0
1501 Great Skuas found dead on Foula (Scotland). This outbreak likely has pop & species level effects with <10% of the population of Scotland affected. This is following substantial outbreaks in 2021 in this species.
Mass Mortality Caused by Highly Pathogenic Influenza A(H5N1) Virus in Sandwich Terns, the Netherlands, 2022
https://wwwnc.cdc.gov/eid/article/28/12/22-1292_article
A summary of the tern outbreaks in the Netherlands out today.
“…out of a total of 18,151 breeding pairs, 8,001 adult Sandwich terns were found dead, and only a few chicks fledged”
Phylogenetic analysis demonstrates two different sublineages of 2.3.4.4b
https://wwwnc.cdc.gov/eid/article/28/12/22-1292_article
The impact of avian influenza 2022 on Dalmatian pelicans was the worst ever wildlife disaster in Greece
https://doi.org/10.1017/S0030605322001041
Probably one of the most poignant paper titles. 2286 pelicans died in Greece due to HPAI, comprising 40% of the SE European pop, and 10% of the global pop.
Calls grow for global avian flu jabs
https://bvajournals.onlinelibrary.wiley.com/doi/epdf/10.1002/vetr.2399
with some good quotes from Les Simms and David Swayne at the @WOAH_Global in Paris, out in Vet Record
H6N8 avian influenza virus in Antarctic seabirds demonstrates connectivity between South America and Antarctica
https://onlinelibrary.wiley.com/doi/10.1111/tbed.14728
Very cool study interrogating an H6N8 avian influenza virus found in penguins and skuas in Antarctica, and through phylogeny, demonstrating viral introduction most likely from South America
Global dissemination of influenza A virus is driven by wild bird migration through arctic and subarctic zones
https://onlinelibrary.wiley.com/doi/10.1111/mec.16738
Detailed study of influenza movement with wild birds, particularly revisiting the recent introduction into North America via the Atlantic shows the importance of bird migration via the arctic and subarctic.
Shift in HPAI infection dynamics causes significant losses in seabird populations across Great Britain
https://bvajournals.onlinelibrary.wiley.com/doi/epdf/10.1002/vetr.2311
Shift in HPAI infection dynamics causes significant losses in seabird populations, here with data from Great Britain. 1454 positive wild birds across 61 species (Although numbers of infected birds expected to be much higher in reality)
Most avian influenza news is coming out of Europe and North America, but it’s a global problem. Emergence of a Reassortant 2.3.4.4b Highly Pathogenic H5N1 Avian Influenza Virus Containing H9N2 PA Gene in Burkina Faso, West Africa, in 2021
https://www.mdpi.com/1999-4915/14/9/1901/htm
New paper from Burkina Faso, with H5N1 viruses detected in clade 2.3.4.4b, closely related to HPAI H5N1 viruses identified in Nigeria and Niger, and H9N2
Long-Term Protective Effect of Serial Infections with H5N8 Highly Pathogenic Avian Influenza Virus in Wild Ducks
https://journals.asm.org/doi/10.1128/jvi.01233-22
What is the impact of repeated waves of HPAI H5 on wild bird populations? Experimental infections show protective immunity of 2014 H5N8 virus in ducks when later exposed to 2016 H5N6. May explain how virus is maintained in wild bird populations
Has Epizootic Become Enzootic? Evidence for a Fundamental Change in the Infection Dynamics of Highly Pathogenic Avian Influenza in Europe, 2021
https://journals.asm.org/doi/10.1128/mbio.00609-22
Huge question: Has Epizootic HPAI H5 Become Enzootic? Importantly, it is likely that we now have continuous circulation in wild birds (genetic drift of circulating viruses) and novel introductions in N Europe. Implications for both prevent and control.
Host diversity and behavior determine patterns of interspecies transmission and geographic diffusion of avian influenza A subtypes among North American wild reservoir species
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009973
A more host specific analysis was recently done in another recent paper, showing lots of transitions within avian orders, but fewer transitions between them. And that transition influenced by breeding habitat range overlap, and not host genetic distance
Maintenance and dissemination of avian-origin influenza A virus within the northern Atlantic Flyway of North America
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1010605
How are avian influenza viruses maintained in multi-species wild bird populations? Complex patterns of virus dissemination between different host groups across many flyways are implicated. Important for surveillance to go beyond Anas ducks.
Disentangling the role of poultry farms and wild birds in the spread of highly pathogenic avian influenza virus in Europe
https://academic.oup.com/ve/article/8/2/veac073/6671198?login=false
Key question in HPAI is the role of wild birds vs poultry. In 2016, HPAI was likely introduced to farms from wild birds, but in many countries epidemic was dominated by farm-to-farm transmission. Key data for prevention, mitigation and control in future
Avian Influenza NS1 Proteins Inhibit Human, but Not Duck, RIG-I Ubiquitination and Interferon Signaling
https://journals.asm.org/doi/epub/10.1128/jvi.00776-22
How does influenza by-pass the host immune response? Here, NS1 from both LPAI and HPAI was shown to inhibit human RIG-I but not duck RIG-I. Differences by NS1 may contribute to the unique disease resistance by avian influenza characteristic of mallards
Avian influenza antibody prevalence increases with mercury contamination in wild waterfowl
https://royalsocietypublishing.org/doi/abs/10.1098/rspb.2022.1312
What modulates the susceptibility of birds to avian influenza infection? Heavy metals, like mercury, play play a key role and explain why we see prevalence differences in closely related species
Influenza A(H11N2) Virus Detection in Fecal Samples from Adélie (Pygoscelis adeliae) and Chinstrap (Pygoscelis antarcticus) Penguins, Penguin Island, Antarctica
https://journals.asm.org/doi/pdf/10.1128/spectrum.01427-22
H11N2 avian influenza viruses have been found again in the Antarctic Peninsula area. Repeat detections over a few years tell us these viruses are endemic. Of interest is that there has been no reassortment with other influenza viruses between years.
Evolutionary features of a prolific subtype of avian influenza A virus in European waterfowl
We wanted to understand what features of influenza allow it to reinfect a population of Mallards year after year.
Lineage replacement and reassortment are key features
https://academic.oup.com/ve/article/8/2/veac074/6700668?searchresult=1
A threat from both sides: Multiple introductions of genetically distinct H5 HPAI viruses into Canada via both East Asia-Australasia/Pacific and Atlantic flyways
https://doi.org/10.1093/ve/veac077
In more #HPAI news, it looks like H5N1 entered North America more than once in 2022. The Atlantic Route being described in Caliendo et al, but recent analysis shows that a secondary incursion also occurred, perhaps over the pacific.
Want to understand how #HPAI is being transmitted? Key paper shows the role of wild bird vs farm-farm transmission in Europe 2016-17
Disentangling the Role of Poultry Farms and Wild Birds in the Spread of Highly Pathogenic Avian Influenza Virus in Europe
https://doi.org/10.1093/ve/veac073
Highly pathogenic avian influenza is an emerging disease threat to wild birds in North America
https://wildlife.onlinelibrary.wiley.com/doi/10.1002/jwmg.22171
For wild birds, lineage 2.3.4.4 H5Nx has been devastating, with thousands dying in mortality events. For example, in early 2021, approximately 10% of Barnacle Geese that breed in Svalbard died due to this disease